Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L42A; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42A has a paralog, RPL42B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 2 | 2 | 4 | 2 | 5 | 0 | 6 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 2 | 2 | 1 | 2 | 0 | 3 | 11 | 8 | 6 | 7 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 1 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 8 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 11 | 14 | 19 | 18 | 9 | 11 | 4 | 1 | 0 | 3 | 3 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 113 | 21 | 94 | 90 | 221 | 305 | 335 | 268 | 166 | 129 | 68 | 104 | 46 | 91 | 147 | 136 | 214 | 242 | 100 | 39 | 104 | 31 | 158 | 142 |
Endoplasmic Reticulum | 1 | 1 | 2 | 1 | 5 | 3 | 6 | 9 | 8 | 4 | 4 | 5 | 0 | 2 | 0 | 0 | 15 | 4 | 1 | 1 | 5 | 0 | 2 | 4 |
Endosome | 0 | 0 | 3 | 2 | 2 | 2 | 6 | 8 | 13 | 6 | 15 | 14 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 12 | 1 | 2 | 3 |
Golgi | 0 | 0 | 28 | 20 | 25 | 7 | 5 | 14 | 2 | 4 | 1 | 3 | 22 | 1 | 0 | 1 | 7 | 3 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 0 | 0 | 1 | 2 | 1 | 13 | 26 | 14 | 104 | 51 | 70 | 83 | 0 | 4 | 2 | 13 | 6 | 0 | 0 | 2 | 2 | 0 | 0 | 3 |
Nucleus | 0 | 0 | 2 | 11 | 3 | 4 | 10 | 26 | 49 | 42 | 41 | 49 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 62 | 16 | 13 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 10 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 23 | 2 | 1 | 3 | 7 | 14 | 5 | 12 | 13 | 9 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 12 | 0 | 18 | 16 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 12 | 4 | 5 | 5 |
Vac/Vac Membrane | 0 | 0 | 162 | 152 | 153 | 111 | 212 | 200 | 133 | 156 | 120 | 171 | 64 | 12 | 4 | 3 | 12 | 4 | 1 | 3 | 22 | 9 | 7 | 6 |
Unique Cell Count | 114 | 23 | 258 | 284 | 380 | 436 | 580 | 518 | 438 | 374 | 286 | 383 | 125 | 110 | 154 | 144 | 229 | 248 | 106 | 52 | 269 | 70 | 219 | 202 |
Labelled Cell Count | 114 | 23 | 295 | 306 | 426 | 466 | 626 | 575 | 518 | 425 | 345 | 459 | 146 | 122 | 157 | 158 | 256 | 255 | 106 | 52 | 269 | 70 | 219 | 202 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 80.8 | 92.0 | 23.7 | 25.1 | 20.7 | 16.1 | 13.7 | 13.5 | 11.1 | 11.1 | 10.0 | 10.2 | 29.0 | 23.7 | 19.5 | 61.0 | 71.1 | 90.5 | 27.4 | 23.6 | 25.7 |
Std Deviation (1e-4) | 15.5 | 21.0 | 15.3 | 17.2 | 13.9 | 11.8 | 8.7 | 8.3 | 6.9 | 6.7 | 5.6 | 5.7 | 19.2 | 15.9 | 12.4 | 23.7 | 29.8 | 31.5 | 19.1 | 13.4 | 21.0 |
Intensity Change (Log2) | – | – | – | 0.09 | -0.19 | -0.56 | -0.79 | -0.81 | -1.09 | -1.1 | -1.25 | -1.21 | 0.29 | 0.0 | -0.28 | 1.36 | 1.59 | 1.94 | 0.21 | -0.0 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 2.9 | 2.9 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.2 | 5.4 | 8.6 | 5.7 | 4.0 | 0.4 | -0.5 | -3.2 | -2.5 | 0.1 | 8.1 | 11.8 | 11.3 | 13.0 | 14.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1.5 | 0 | 2.7 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.6 | -1.9 | -5.4 | -6.9 | -4.7 | -6.5 | -5.5 | -5.4 | -5.8 | 1.8 | -3.2 | -4.2 | -3.8 | -3.3 | -4.5 |
Mitochondria | 0 | 0 | 2.3 | 3.1 | 0 | 8.3 | 6.0 | 8.3 | 7.8 | 0 | 0 | 0 | 4.5 | 0 | 0 |
Nucleus | 2.4 | 0 | 0 | 0 | 3.0 | 5.1 | 5.1 | 5.9 | 5.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4.7 | 0 | 0 | 0 | 0 | 2.9 | 0 | 3.3 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.2 | -5.6 | -9.7 | -7.1 | -6.4 | -8.4 | -5.2 | -4.9 | -4.5 | -2.2 | -9.1 | -12.1 | -11.9 | -13.2 | -14.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 34.7971 | 45.1004 | 45.8887 | 42.8744 | 33.6219 | 43.8163 | 46.1713 | 55.6768 | 59.9995 | 35.0979 | 62.7976 | 44.3867 | 37.5135 | 45.2074 | 33.7152 | 34.3297 | 42.3604 | 40.3416 |
Actin | 0.0303 | 0.0006 | 0.0025 | 0.0017 | 0.0761 | 0.0011 | 0.0873 | 0.0005 | 0.0051 | 0.0003 | 0.0102 | 0.0002 | 0.1143 | 0.0001 | 0.0194 | 0.0013 | 0.0068 | 0.0107 |
Bud | 0.0012 | 0.0005 | 0.001 | 0.0011 | 0.0032 | 0.0014 | 0.0046 | 0.0008 | 0.0026 | 0.0013 | 0.0015 | 0.0003 | 0.0015 | 0.0003 | 0.001 | 0.0006 | 0.0005 | 0.0004 |
Bud Neck | 0.0013 | 0.0008 | 0.0008 | 0.0008 | 0.029 | 0.002 | 0.0016 | 0.0008 | 0.0015 | 0.0013 | 0.0019 | 0.0004 | 0.0017 | 0.0005 | 0.0008 | 0.0066 | 0.001 | 0.0022 |
Bud Periphery | 0.002 | 0.0002 | 0.0004 | 0.0005 | 0.0049 | 0.001 | 0.0046 | 0.0002 | 0.0032 | 0.0009 | 0.0008 | 0.0001 | 0.0019 | 0.0001 | 0.0009 | 0.0004 | 0.0003 | 0.0005 |
Bud Site | 0.0089 | 0.0008 | 0.0009 | 0.0005 | 0.0068 | 0.0002 | 0.0082 | 0.001 | 0.0061 | 0.0006 | 0.002 | 0.0001 | 0.0032 | 0.0004 | 0.0076 | 0.0085 | 0.0008 | 0.0001 |
Cell Periphery | 0.0021 | 0.0016 | 0.0021 | 0.0006 | 0.004 | 0.0005 | 0.0059 | 0.0009 | 0.012 | 0.0002 | 0.0018 | 0.0002 | 0.0023 | 0.0001 | 0.0006 | 0.0007 | 0.0002 | 0.0004 |
Cytoplasm | 0.2431 | 0.4226 | 0.3322 | 0.3657 | 0.1415 | 0.2651 | 0.3984 | 0.5409 | 0.4869 | 0.3287 | 0.6193 | 0.3821 | 0.4734 | 0.3925 | 0.1545 | 0.2677 | 0.3773 | 0.314 |
Cytoplasmic Foci | 0.0926 | 0.0028 | 0.0021 | 0.0268 | 0.0083 | 0.0126 | 0.0369 | 0.0033 | 0.0045 | 0.0013 | 0.0302 | 0.009 | 0.0492 | 0.0006 | 0.0184 | 0.0024 | 0.0091 | 0.0018 |
Eisosomes | 0.0032 | 0 | 0.0001 | 0 | 0.0027 | 0 | 0.0012 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0012 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0132 | 0.0018 | 0.0015 | 0.0014 | 0.0059 | 0.0221 | 0.024 | 0.0017 | 0.0042 | 0.0015 | 0.0033 | 0.0006 | 0.0229 | 0.0003 | 0.0022 | 0.0006 | 0.0201 | 0.0075 |
Endosome | 0.0389 | 0.0089 | 0.0031 | 0.0093 | 0.0112 | 0.0069 | 0.0308 | 0.0044 | 0.0055 | 0.0336 | 0.0264 | 0.0026 | 0.0327 | 0.0004 | 0.0061 | 0.0072 | 0.0292 | 0.0033 |
Golgi | 0.0119 | 0.003 | 0.0009 | 0.0052 | 0.0071 | 0.0019 | 0.0131 | 0.0002 | 0.0009 | 0.0016 | 0.0401 | 0.0001 | 0.0293 | 0 | 0.0032 | 0.0024 | 0.0045 | 0.0008 |
Lipid Particles | 0.0411 | 0.0013 | 0.0007 | 0.0202 | 0.0178 | 0.0069 | 0.0098 | 0.0005 | 0.0008 | 0.0002 | 0.0103 | 0.0028 | 0.0441 | 0 | 0.0217 | 0.0025 | 0.0153 | 0.0004 |
Mitochondria | 0.0581 | 0.0019 | 0.0174 | 0.0219 | 0.0179 | 0.008 | 0.0083 | 0.0008 | 0.0078 | 0.0011 | 0.0136 | 0.0047 | 0.0244 | 0.0003 | 0.0148 | 0.0004 | 0.0036 | 0.0009 |
None | 0.081 | 0.0003 | 0.0008 | 0.0009 | 0.0038 | 0.0003 | 0.0343 | 0.0023 | 0.0011 | 0.0003 | 0.024 | 0.0104 | 0.0182 | 0.0002 | 0.0017 | 0.0003 | 0.001 | 0.0004 |
Nuclear Periphery | 0.0923 | 0.0344 | 0.0437 | 0.051 | 0.055 | 0.125 | 0.07 | 0.0214 | 0.0229 | 0.0191 | 0.0106 | 0.0452 | 0.0396 | 0.022 | 0.0203 | 0.0512 | 0.0524 | 0.0802 |
Nucleolus | 0.0116 | 0.0132 | 0.0646 | 0.0132 | 0.0111 | 0.0021 | 0.0179 | 0.0228 | 0.0427 | 0.0594 | 0.0363 | 0.0245 | 0.0055 | 0.0259 | 0.0316 | 0.0386 | 0.0224 | 0.0033 |
Nucleus | 0.1882 | 0.4838 | 0.4585 | 0.4355 | 0.5079 | 0.4946 | 0.1331 | 0.3681 | 0.3421 | 0.5111 | 0.1183 | 0.4916 | 0.0772 | 0.5506 | 0.6362 | 0.5932 | 0.4118 | 0.5555 |
Peroxisomes | 0.0275 | 0.0001 | 0.0006 | 0.0082 | 0.005 | 0.0002 | 0.0053 | 0.0001 | 0.0006 | 0.0001 | 0.002 | 0.0004 | 0.0183 | 0 | 0.0253 | 0.0002 | 0.0298 | 0.0013 |
Punctate Nuclear | 0.0149 | 0.0023 | 0.0238 | 0.0037 | 0.003 | 0.0053 | 0.0468 | 0.0122 | 0.0084 | 0.0028 | 0.0039 | 0.0017 | 0.0092 | 0.0015 | 0.0164 | 0.0034 | 0.0046 | 0.0021 |
Vacuole | 0.0197 | 0.015 | 0.0337 | 0.0216 | 0.0592 | 0.0236 | 0.0498 | 0.0145 | 0.0347 | 0.0261 | 0.0341 | 0.0121 | 0.0185 | 0.0032 | 0.0131 | 0.0074 | 0.0069 | 0.0104 |
Vacuole Periphery | 0.0169 | 0.0042 | 0.0086 | 0.0104 | 0.0186 | 0.0191 | 0.0082 | 0.0024 | 0.0065 | 0.0086 | 0.0093 | 0.0107 | 0.0113 | 0.001 | 0.0039 | 0.0042 | 0.0023 | 0.0038 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2729.2932 | 3106.624 | 3853.4826 | 2797.2896 | 3342.5516 | 3019.7993 | 2571.7598 | 2481.7606 | 2781.9252 | 2392.1866 |
Translational Efficiency | 1.2015 | 1.1373 | 0.9335 | 1.2059 | 1.2327 | 1.0053 | 1.0566 | 1.1924 | 0.984 | 1.1704 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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