Standard name | |
---|---|
Human Ortholog | |
Description | Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | 5 | 9 | 12 | 3 | 3 | 11 |
Bud | 1 | 3 | 13 | 8 | 5 | 8 | – | 14 | 16 | 25 | 40 | 34 | 1 | 2 | 0 | 1 | – | – | 1 | 5 | 3 | 2 | 2 | 7 |
Bud Neck | 1 | 0 | 5 | 0 | 0 | 0 | – | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | – | – | 4 | 1 | 2 | 0 | 0 | 8 |
Bud Site | 0 | 2 | 1 | 1 | 2 | 7 | – | 8 | 8 | 14 | 6 | 14 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 6 | 2 | 2 | 1 | 0 | – | 3 | 0 | 2 | 4 | 4 | 1 | 0 | 1 | 2 | – | – | 1 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 109 | 73 | 107 | 64 | 65 | 60 | – | 57 | 51 | 71 | 65 | 63 | 67 | 89 | 98 | 59 | – | – | 59 | 10 | 23 | 2 | 6 | 20 |
Endoplasmic Reticulum | 3 | 4 | 4 | 0 | 3 | 2 | – | 1 | 1 | 0 | 2 | 0 | 8 | 0 | 6 | 3 | – | – | 2 | 3 | 2 | 0 | 0 | 1 |
Endosome | 3 | 11 | 0 | 1 | 25 | 6 | – | 2 | 0 | 0 | 2 | 2 | 3 | 16 | 15 | 3 | – | – | 15 | 18 | 30 | 6 | 11 | 12 |
Golgi | 0 | 1 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | – | 0 | 4 | 2 | 1 | 2 | 3 |
Mitochondria | 41 | 67 | 191 | 189 | 144 | 277 | – | 435 | 384 | 432 | 460 | 506 | 14 | 19 | 6 | 8 | – | – | 81 | 160 | 217 | 64 | 93 | 104 |
Nucleus | 1 | 0 | 1 | 1 | 1 | 9 | – | 8 | 5 | 8 | 7 | 4 | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | – | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | 0 | 1 | 2 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 1 | 1 | 2 | 2 | – | 3 | 0 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2 | 1 | 6 | 12 | 3 | – | 4 | 0 | 2 | 8 | 5 | 0 | 2 | 5 | 0 | – | – | 0 | 4 | 10 | 3 | 4 | 1 |
SpindlePole | 5 | 10 | 12 | 4 | 12 | 10 | – | 29 | 22 | 22 | 42 | 30 | 2 | 1 | 2 | 0 | – | – | 6 | 5 | 8 | 2 | 5 | 6 |
Vac/Vac Membrane | 4 | 17 | 6 | 4 | 6 | 14 | – | 10 | 8 | 8 | 6 | 11 | 5 | 10 | 9 | 0 | – | – | 17 | 9 | 13 | 2 | 11 | 25 |
Unique Cell Count | 154 | 168 | 292 | 232 | 233 | 343 | 501 | 422 | 486 | 521 | 554 | 99 | 130 | 131 | 68 | 204 | 239 | 342 | 96 | 148 | 209 | |||
Labelled Cell Count | 170 | 197 | 346 | 282 | 279 | 400 | 580 | 496 | 590 | 646 | 675 | 103 | 141 | 143 | 79 | 204 | 239 | 342 | 96 | 148 | 209 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 4.9 | 3.3 | 3.4 | 4.1 | 3.4 | – | 3.3 | 2.9 | 3.2 | 3.0 | 3.1 | 4.7 | 5.0 | 4.7 | 5.5 | – | 4.8 | 4.4 | 5.1 | 5.0 |
Std Deviation (1e-4) | 0.4 | 1.6 | 0.9 | 1.8 | 1.7 | 1.6 | – | 1.0 | 1.1 | 1.0 | 1.3 | 1.0 | 0.9 | 1.5 | 0.9 | 2.0 | – | 1.2 | 0.5 | 1.2 | 0.9 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.3 | 0.05 | – | -0.0 | -0.19 | -0.06 | -0.13 | -0.12 | 0.5 | 0.6 | 0.5 | 0.72 | – | 0.53 | 0.39 | 0.62 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Cytoplasm | -2.2 | -2.1 | -5.5 | – | -8.5 | -7.8 | -7.1 | -8.1 | -8.7 | 5.4 | 6.1 | 7.3 | 7.5 | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -9.6 | 0 | -8.0 | – | – |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – |
Vacuole | 0 | 0.4 | 1.5 | – | -0.1 | -0.2 | -0.4 | 0 | -0.1 | 0 | 0 | 0 | 0 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1244 | 0.2657 | 0.144 | -0.1609 | -0.312 | 0.1111 | -0.5189 | 0.1228 | -0.1427 | -0.1574 | -0.2414 | -0.0512 | 2.3898 | 2.3924 | 3.0986 | 2.3592 | 2.8282 | 2.2998 |
Actin | 0.003 | 0.0102 | 0.0078 | 0.0042 | 0.0093 | 0.0045 | 0.0082 | 0.0116 | 0.0047 | 0.0088 | 0.0181 | 0.0083 | 0.0075 | 0.0093 | 0.0031 | 0.0019 | 0.0073 | 0.0036 |
Bud | 0.0037 | 0.0036 | 0.0031 | 0.0079 | 0.0024 | 0.0028 | 0.0019 | 0.0044 | 0.0031 | 0.0029 | 0.0024 | 0.0018 | 0.0014 | 0.006 | 0.0037 | 0.0014 | 0.0041 | 0.0019 |
Bud Neck | 0.0034 | 0.0018 | 0.0023 | 0.0068 | 0.0014 | 0.0123 | 0.0058 | 0.0027 | 0.0031 | 0.0029 | 0.0036 | 0.0085 | 0.0079 | 0.0016 | 0.0038 | 0.0019 | 0.002 | 0.0033 |
Bud Periphery | 0.0041 | 0.0088 | 0.0068 | 0.0226 | 0.0079 | 0.0044 | 0.0056 | 0.0079 | 0.0076 | 0.0097 | 0.0064 | 0.0043 | 0.0048 | 0.0187 | 0.011 | 0.0025 | 0.0078 | 0.0056 |
Bud Site | 0.0068 | 0.0447 | 0.0117 | 0.0247 | 0.0018 | 0.0025 | 0.0089 | 0.0455 | 0.0166 | 0.0029 | 0.0065 | 0.0021 | 0.0081 | 0.0234 | 0.0239 | 0.0014 | 0.0044 | 0.0017 |
Cell Periphery | 0.0007 | 0.0029 | 0.0012 | 0.0013 | 0.0004 | 0.0007 | 0.0011 | 0.0016 | 0.0016 | 0.0013 | 0.0006 | 0.0005 | 0.0016 | 0.0011 | 0.0011 | 0.0002 | 0.0079 | 0.0009 |
Cytoplasm | 0.0451 | 0.0529 | 0.0591 | 0.0259 | 0.0538 | 0.0502 | 0.0566 | 0.0454 | 0.0617 | 0.057 | 0.0515 | 0.0628 | 0.0636 | 0.0645 | 0.0465 | 0.0204 | 0.0393 | 0.0509 |
Cytoplasmic Foci | 0.1588 | 0.1826 | 0.1371 | 0.1554 | 0.1603 | 0.1765 | 0.1628 | 0.1305 | 0.1495 | 0.1291 | 0.1392 | 0.1402 | 0.1182 | 0.1453 | 0.1477 | 0.2042 | 0.1503 | 0.1009 |
Eisosomes | 0.0005 | 0.0007 | 0.0006 | 0.0005 | 0.0003 | 0.0003 | 0.0009 | 0.0005 | 0.001 | 0.0003 | 0.0004 | 0.0004 | 0.0011 | 0.0004 | 0.0004 | 0.0001 | 0.006 | 0.0003 |
Endoplasmic Reticulum | 0.0027 | 0.003 | 0.0027 | 0.0034 | 0.0026 | 0.0021 | 0.0037 | 0.0033 | 0.0035 | 0.002 | 0.0019 | 0.0027 | 0.0045 | 0.0021 | 0.0026 | 0.0012 | 0.0047 | 0.0014 |
Endosome | 0.1757 | 0.1416 | 0.1184 | 0.1356 | 0.1444 | 0.1278 | 0.1867 | 0.162 | 0.1142 | 0.1037 | 0.1317 | 0.1621 | 0.1759 | 0.1701 | 0.1464 | 0.177 | 0.1419 | 0.1353 |
Golgi | 0.0567 | 0.0497 | 0.0353 | 0.0329 | 0.0539 | 0.0466 | 0.0509 | 0.0726 | 0.0418 | 0.0403 | 0.0454 | 0.0538 | 0.0579 | 0.0531 | 0.0512 | 0.0525 | 0.0367 | 0.048 |
Lipid Particles | 0.0346 | 0.019 | 0.0196 | 0.0377 | 0.0486 | 0.0383 | 0.031 | 0.0115 | 0.0229 | 0.0082 | 0.0161 | 0.0318 | 0.0293 | 0.018 | 0.018 | 0.023 | 0.0138 | 0.0215 |
Mitochondria | 0.2648 | 0.253 | 0.3456 | 0.3178 | 0.2232 | 0.27 | 0.2554 | 0.2962 | 0.2914 | 0.421 | 0.3178 | 0.295 | 0.3362 | 0.3156 | 0.3053 | 0.2921 | 0.334 | 0.3874 |
None | 0.1479 | 0.1318 | 0.1667 | 0.1363 | 0.1758 | 0.133 | 0.1448 | 0.1366 | 0.171 | 0.0915 | 0.1551 | 0.1294 | 0.0993 | 0.0904 | 0.1288 | 0.0502 | 0.1327 | 0.151 |
Nuclear Periphery | 0.0019 | 0.0014 | 0.0019 | 0.0026 | 0.0043 | 0.0011 | 0.0021 | 0.001 | 0.0024 | 0.0006 | 0.0012 | 0.002 | 0.002 | 0.0011 | 0.0029 | 0.0004 | 0.0026 | 0.0005 |
Nucleolus | 0.0034 | 0.0031 | 0.0033 | 0.0044 | 0.0059 | 0.0026 | 0.0014 | 0.002 | 0.0024 | 0.0007 | 0.0016 | 0.0014 | 0.0013 | 0.0036 | 0.0056 | 0.0003 | 0.001 | 0.0007 |
Nucleus | 0.0029 | 0.003 | 0.0041 | 0.0044 | 0.0082 | 0.0028 | 0.0025 | 0.0024 | 0.003 | 0.0014 | 0.0029 | 0.0028 | 0.0022 | 0.0025 | 0.007 | 0.0005 | 0.0022 | 0.0014 |
Peroxisomes | 0.0603 | 0.0697 | 0.0545 | 0.0487 | 0.0779 | 0.1039 | 0.0501 | 0.0427 | 0.0827 | 0.0951 | 0.0798 | 0.071 | 0.0616 | 0.0547 | 0.0679 | 0.1611 | 0.0828 | 0.0739 |
Punctate Nuclear | 0.0054 | 0.0019 | 0.0046 | 0.015 | 0.0069 | 0.007 | 0.0024 | 0.0014 | 0.0035 | 0.0009 | 0.0061 | 0.0036 | 0.001 | 0.0055 | 0.0051 | 0.001 | 0.0018 | 0.0011 |
Vacuole | 0.0138 | 0.0116 | 0.0106 | 0.0084 | 0.0074 | 0.0075 | 0.0129 | 0.0149 | 0.0093 | 0.0147 | 0.0082 | 0.0109 | 0.0101 | 0.0089 | 0.0134 | 0.0042 | 0.0124 | 0.0056 |
Vacuole Periphery | 0.0039 | 0.0031 | 0.0032 | 0.0035 | 0.0035 | 0.003 | 0.0041 | 0.0033 | 0.0031 | 0.005 | 0.0035 | 0.0046 | 0.0045 | 0.0041 | 0.0045 | 0.0024 | 0.0044 | 0.0031 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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