Standard name | |
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Human Ortholog | |
Description | Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments; human gene CARS allows growth of the yeast haploid null mutant after sporulation of a heterozygous diploid |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 6 | 12 | 11 | 11 | 15 | 11 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 2 | 1 | 1 | 0 | 3 | 7 | 5 | 2 | 3 | 12 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 3 | 5 | 12 | 6 | 14 | 18 | 19 | 21 | 33 | 34 | 36 | 10 | 14 | 27 | 10 | 14 | 13 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 206 | 150 | 110 | 232 | 308 | 300 | 325 | 341 | 241 | 308 | 212 | 313 | 164 | 370 | 424 | 209 | 252 | 297 | 204 | 146 | 119 | 164 | 249 | 296 |
Endoplasmic Reticulum | 9 | 1 | 2 | 1 | 11 | 31 | 52 | 80 | 36 | 62 | 47 | 38 | 11 | 21 | 8 | 7 | 8 | 12 | 1 | 2 | 0 | 7 | 14 | 16 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 1 | 0 | 0 | 2 | 5 | 15 | 60 | 52 | 95 | 78 | 83 | 117 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 2 |
Nucleus | 0 | 1 | 0 | 1 | 3 | 8 | 3 | 8 | 4 | 5 | 1 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 1 | 0 | 0 | 7 | 18 | 7 | 9 | 7 | 13 | 0 | 4 | 1 | 0 | 7 | 1 | 0 | 0 | 4 | 7 | 15 | 15 |
Unique Cell Count | 212 | 152 | 116 | 240 | 326 | 347 | 430 | 494 | 374 | 455 | 328 | 471 | 175 | 392 | 440 | 212 | 262 | 304 | 208 | 156 | 132 | 186 | 289 | 343 |
Labelled Cell Count | 223 | 156 | 119 | 250 | 334 | 374 | 482 | 540 | 422 | 514 | 399 | 547 | 186 | 411 | 464 | 228 | 283 | 323 | 208 | 156 | 132 | 186 | 289 | 343 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 25.3 | 28.1 | 16.7 | 18.6 | 15.3 | 14.0 | 12.3 | 12.4 | 11.1 | 10.9 | 11.1 | 10.7 | 24.3 | 23.9 | 25.1 | 29.9 | 29.1 | 31.5 | 26.7 | 27.0 | 28.1 |
Std Deviation (1e-4) | 3.9 | 5.7 | 3.0 | 3.3 | 2.8 | 2.8 | 2.7 | 2.9 | 2.6 | 2.5 | 2.8 | 2.6 | 4.9 | 5.1 | 4.9 | 7.5 | 6.8 | 5.9 | 5.2 | 6.1 | 6.6 |
Intensity Change (Log2) | – | – | – | 0.16 | -0.13 | -0.26 | -0.44 | -0.44 | -0.59 | -0.62 | -0.6 | -0.64 | 0.54 | 0.51 | 0.58 | 0.84 | 0.8 | 0.91 | 0.67 | 0.69 | 0.75 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.3 | 0 | 0 | 0 | 0 | 0.5 | 1.1 | 2.0 | 1.3 | 0.5 | 0 | 0.8 | 0.2 | 0.4 | 0 |
Cytoplasm | 0.8 | -0.1 | -2.5 | -4.6 | -5.7 | -6.3 | -5.9 | -6.3 | -6.1 | -0.4 | -0.2 | 0.8 | 2.0 | 0.6 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 2.6 | 3.3 | 4.1 | 2.8 | 3.6 | 3.7 | 2.4 | 1.8 | 1.7 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.3 | 3.7 | 6.0 | 4.8 | 6.0 | 6.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0005 | 0.0004 | 0.0004 | 0.0005 | 0.002 | 0.0016 | 0.0062 | 0.0004 | 0.0006 | 0.0005 | 0.0005 | 0.001 | 0.0004 | 0.0005 | 0.001 | 0.0159 | 0.0019 | 0.0013 |
Bud | 0.0005 | 0.0004 | 0.0008 | 0.001 | 0.001 | 0.0013 | 0.0008 | 0.0004 | 0.0008 | 0.0015 | 0.0002 | 0.0008 | 0.0006 | 0.0005 | 0.001 | 0.0135 | 0.0001 | 0.0005 |
Bud Neck | 0.0006 | 0.0005 | 0.0009 | 0.0007 | 0.0014 | 0.002 | 0.0004 | 0.0003 | 0.0005 | 0.0007 | 0.0007 | 0.0013 | 0.0001 | 0.0002 | 0.0005 | 0.0015 | 0.0004 | 0.0006 |
Bud Periphery | 0.0007 | 0.0002 | 0.0008 | 0.0011 | 0.0013 | 0.002 | 0.0006 | 0.0002 | 0.0006 | 0.0008 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0125 | 0 | 0.0001 |
Bud Site | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0001 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0009 | 0.0073 | 0 | 0.0001 |
Cell Periphery | 0.0019 | 0.0015 | 0.0011 | 0.0006 | 0.0016 | 0.002 | 0.0006 | 0.0006 | 0.0005 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0005 | 0 |
Cytoplasm | 0.8343 | 0.9399 | 0.9075 | 0.8057 | 0.798 | 0.8121 | 0.8463 | 0.951 | 0.9335 | 0.8613 | 0.8977 | 0.8676 | 0.9251 | 0.9888 | 0.9166 | 0.5665 | 0.9704 | 0.9257 |
Cytoplasmic Foci | 0.0048 | 0.0037 | 0.0045 | 0.0082 | 0.0118 | 0.0096 | 0.0102 | 0.0047 | 0.0067 | 0.0064 | 0.0078 | 0.0109 | 0.0019 | 0.0017 | 0.0053 | 0.0256 | 0.003 | 0.0105 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0907 | 0.0293 | 0.0385 | 0.0409 | 0.076 | 0.1028 | 0.0546 | 0.016 | 0.0151 | 0.0083 | 0.0416 | 0.0585 | 0.0256 | 0.0008 | 0.0126 | 0.0087 | 0.016 | 0.0141 |
Endosome | 0.02 | 0.0081 | 0.014 | 0.0447 | 0.0499 | 0.0302 | 0.0363 | 0.0086 | 0.0195 | 0.0591 | 0.0341 | 0.0323 | 0.0135 | 0.0022 | 0.0398 | 0.1234 | 0.0033 | 0.0338 |
Golgi | 0.0018 | 0.0018 | 0.0016 | 0.0029 | 0.0072 | 0.0063 | 0.0043 | 0.0018 | 0.0028 | 0.0046 | 0.0037 | 0.0087 | 0.0005 | 0.0003 | 0.0017 | 0.0661 | 0.0006 | 0.0042 |
Lipid Particles | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0 | 0 | 0 | 0 | 0.0065 | 0.0009 | 0 |
Mitochondria | 0.0023 | 0.0009 | 0.0012 | 0.0061 | 0.0035 | 0.014 | 0.0049 | 0.0007 | 0.0041 | 0.0026 | 0.0021 | 0.0089 | 0.0023 | 0.0004 | 0.0015 | 0.0248 | 0 | 0.0028 |
None | 0.0006 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0007 | 0.0024 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0005 | 0.0007 | 0.0002 | 0.0004 | 0.0009 | 0.0001 | 0.0004 |
Nuclear Periphery | 0.0059 | 0.0014 | 0.003 | 0.0042 | 0.0052 | 0.0019 | 0.0049 | 0.0006 | 0.0013 | 0.0037 | 0.0025 | 0.0012 | 0.0132 | 0.0004 | 0.0017 | 0.007 | 0.0008 | 0.0012 |
Nucleolus | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0 |
Nucleus | 0.0027 | 0.0009 | 0.0017 | 0.0016 | 0.0015 | 0.0013 | 0.0019 | 0.0005 | 0.0008 | 0.0018 | 0.0009 | 0.0006 | 0.0041 | 0.0007 | 0.0011 | 0.0031 | 0.0003 | 0.0008 |
Peroxisomes | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0036 | 0 | 0.0001 |
Punctate Nuclear | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0006 | 0.0001 | 0 |
Vacuole | 0.0279 | 0.0085 | 0.0204 | 0.0663 | 0.0308 | 0.0089 | 0.0185 | 0.0123 | 0.0096 | 0.0362 | 0.0053 | 0.0041 | 0.0078 | 0.0023 | 0.0094 | 0.0827 | 0.0013 | 0.002 |
Vacuole Periphery | 0.004 | 0.0011 | 0.0024 | 0.0143 | 0.0078 | 0.0029 | 0.0046 | 0.001 | 0.0025 | 0.0108 | 0.002 | 0.0023 | 0.0036 | 0.0004 | 0.0061 | 0.0276 | 0.0001 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 132.897 | 142.9903 | 117.5414 | 125.2262 | 124.1851 | 116.7889 | 112.1124 | 99.9172 | 93.2555 | 119.2091 |
Translational Efficiency | 1.5144 | 1.4173 | 1.4369 | 1.1437 | 1.2741 | 1.3529 | 1.3611 | 1.1653 | 1.1839 | 1.1489 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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