Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 4 | 3 | 4 | 5 | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 4 | 5 | 5 | 9 | 6 | 10 | 7 | 4 | 2 | 6 | 22 | 23 |
Bud | 3 | 0 | 1 | 4 | 1 | 7 | 10 | 7 | 5 | 6 | 9 | 6 | 4 | 3 | 1 | 3 | 4 | 8 | 0 | 0 | 0 | 1 | 5 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 6 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | – | – | – | – | – | – |
Cell Periphery | 8 | 7 | 7 | 5 | 7 | 4 | 6 | 4 | 2 | 0 | 0 | 5 | 14 | 15 | 22 | 23 | 11 | 11 | 1 | 1 | 0 | 0 | 1 | 2 |
Cytoplasm | 0 | 0 | 1 | 4 | 2 | 8 | 8 | 5 | 9 | 5 | 6 | 5 | 6 | 11 | 18 | 9 | 6 | 11 | 0 | 0 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 2 | 0 | 2 | 1 | 1 | 3 | 1 | 2 | 1 | 3 | 0 | 0 | 2 | 3 | 3 | 6 | 2 | 3 | 0 | 0 | 0 | 0 | 2 | 2 |
Endosome | 2 | 4 | 6 | 0 | 10 | 3 | 7 | 4 | 0 | 0 | 0 | 3 | 20 | 21 | 16 | 0 | 4 | 3 | 12 | 7 | 2 | 7 | 23 | 19 |
Golgi | 0 | 10 | 31 | 18 | 40 | 4 | 0 | 8 | 1 | 2 | 0 | 4 | 135 | 300 | 394 | 145 | 34 | 22 | 10 | 16 | 10 | 28 | 34 | 36 |
Mitochondria | 388 | 177 | 118 | 120 | 203 | 231 | 350 | 255 | 214 | 194 | 166 | 185 | 405 | 489 | 402 | 25 | 9 | 9 | 372 | 163 | 139 | 277 | 436 | 506 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 2 | 1 | 3 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 2 |
Nuclear Periphery | 12 | 11 | 8 | 4 | 11 | 35 | 50 | 23 | 23 | 21 | 17 | 25 | 23 | 29 | 24 | 6 | 6 | 3 | 4 | 1 | 1 | 3 | 7 | 11 |
Nucleolus | 32 | 8 | 5 | 5 | 4 | 20 | 54 | 22 | 26 | 21 | 15 | 15 | 16 | 7 | 8 | 4 | 10 | 5 | 2 | 0 | 0 | 3 | 2 | 3 |
Peroxisomes | 14 | 4 | 10 | 5 | 5 | 3 | 6 | 5 | 3 | 0 | 0 | 2 | 33 | 40 | 24 | 12 | 22 | 16 | 18 | 3 | 7 | 44 | 57 | 54 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 4 | 11 | 7 | 0 | 4 | 1 | 5 | 2 | 5 | 5 | 4 | 2 | 0 | 0 | 0 | 0 | 1 | 3 | 3 |
Vac/Vac Membrane | 33 | 20 | 5 | 6 | 25 | 18 | 19 | 11 | 10 | 9 | 7 | 11 | 55 | 78 | 95 | 44 | 19 | 14 | 2 | 5 | 0 | 4 | 13 | 9 |
Unique Cell Count | 411 | 192 | 139 | 136 | 237 | 266 | 403 | 291 | 245 | 219 | 184 | 213 | 500 | 684 | 704 | 238 | 119 | 104 | 438 | 207 | 168 | 382 | 621 | 682 |
Labelled Cell Count | 497 | 245 | 198 | 176 | 315 | 342 | 530 | 360 | 302 | 269 | 227 | 268 | 719 | 1007 | 1018 | 292 | 137 | 120 | 438 | 207 | 168 | 382 | 621 | 682 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.7 | 12.2 | 10.4 | 9.6 | 10.1 | 9.1 | 8.3 | 8.7 | 7.6 | 7.9 | 7.0 | 8.1 | 11.1 | 11.4 | 11.1 | 15.6 | 15.0 | 14.9 | 11.6 | 11.4 | 11.5 |
Std Deviation (1e-4) | 2.0 | 2.6 | 2.0 | 1.9 | 1.7 | 2.0 | 1.9 | 1.9 | 2.0 | 1.9 | 1.9 | 1.9 | 1.8 | 1.7 | 1.6 | 3.6 | 3.6 | 4.1 | 2.1 | 2.1 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.05 | -0.2 | -0.32 | -0.26 | -0.45 | -0.41 | -0.57 | -0.37 | 0.09 | 0.13 | 0.09 | 0.58 | 0.52 | 0.52 | 0.15 | 0.12 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.6 | -1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 1.3 | 1.6 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.0 | -1.3 | -7.1 | -9.8 | -6.6 | -7.5 | -6.8 | -6.7 | -6.3 | 1.1 | 4.7 | 7.3 | 7.3 | 1.2 | -0.2 |
Mitochondria | 0.8 | 0.2 | 0.5 | 0.6 | 0.8 | 0.7 | 1.0 | 1.5 | 0.5 | -1.1 | -3.3 | -6.2 | -14.4 | -12.4 | -11.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.1 | -0.5 | 2.3 | 2.2 | 0.8 | 1.3 | 1.3 | 1.2 | 1.9 | -0.6 | -0.8 | -1.3 | -1.6 | -0.3 | 0 |
Nucleolus | 0 | 0 | 1.6 | 3.2 | 1.6 | 2.4 | 2.1 | 1.7 | 1.4 | 0 | 0 | 0 | 0 | 1.6 | 0 |
Peroxisomes | -1.3 | -2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | -0.6 | -2.1 | -0.9 | 2.7 | 2.0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.3 | 2.4 | 1.3 | 0.6 | 0.1 | 0.2 | 0.2 | 0.1 | 0.7 | 2.6 | 2.8 | 3.3 | 4.1 | 3.4 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.536 | 7.6311 | 7.6164 | 7.2323 | 7.0116 | 7.0055 | 10.2572 | 9.7816 | 9.8463 | 9.0494 | 8.1267 | 10.0294 | 12.2607 | 11.2072 | 11.3898 | 10.8206 | 10.788 | 11.2273 |
Actin | 0.0558 | 0.0135 | 0.0134 | 0.0001 | 0.0063 | 0.0048 | 0.0154 | 0.0109 | 0.0016 | 0.01 | 0.019 | 0.0006 | 0.0023 | 0.0128 | 0.0009 | 0.0009 | 0.0075 | 0.0009 |
Bud | 0.0007 | 0.0018 | 0.0007 | 0 | 0.001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0037 | 0.001 | 0.0008 | 0 | 0.0007 | 0.0002 | 0.0014 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Periphery | 0.0012 | 0.0031 | 0.0022 | 0.0001 | 0.0025 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0023 | 0.0013 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.001 | 0.0004 | 0.0001 |
Bud Site | 0.0044 | 0.0086 | 0.0035 | 0 | 0.0021 | 0.0002 | 0.0012 | 0.0005 | 0.0004 | 0.0009 | 0.0046 | 0 | 0.0001 | 0.0006 | 0.0017 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0006 | 0.0037 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.0039 | 0.0009 | 0.0004 | 0 | 0.0014 | 0.0002 | 0.0014 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0013 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0071 | 0.0045 | 0.0037 | 0.0001 | 0.0035 | 0.0051 | 0.0081 | 0.0016 | 0.001 | 0.0006 | 0.0066 | 0.0003 | 0.0015 | 0.0024 | 0.0005 | 0.0015 | 0.0021 | 0.0005 |
Eisosomes | 0.0007 | 0.0003 | 0.0009 | 0.0001 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0045 | 0.0003 | 0.0001 | 0 | 0.0013 | 0.0004 | 0.0003 | 0.0001 | 0.0005 | 0.0009 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0023 | 0.0001 |
Endosome | 0.0254 | 0.014 | 0.0044 | 0.0006 | 0.0157 | 0.0122 | 0.0064 | 0.0075 | 0.0061 | 0.0048 | 0.0237 | 0.0014 | 0.0085 | 0.0052 | 0.0018 | 0.0111 | 0.0183 | 0.0033 |
Golgi | 0.0238 | 0.0242 | 0.0074 | 0.0008 | 0.0043 | 0.0224 | 0.0107 | 0.02 | 0.014 | 0.0044 | 0.0303 | 0.0114 | 0.013 | 0.0165 | 0.0059 | 0.0604 | 0.0508 | 0.0153 |
Lipid Particles | 0.0054 | 0.0091 | 0.0069 | 0.0002 | 0.0114 | 0.0103 | 0.0147 | 0.0072 | 0.0036 | 0.0019 | 0.0069 | 0.0064 | 0.0187 | 0.0054 | 0.0024 | 0.0007 | 0.0111 | 0.0018 |
Mitochondria | 0.7924 | 0.8651 | 0.9412 | 0.9936 | 0.8698 | 0.9261 | 0.9074 | 0.9335 | 0.9641 | 0.933 | 0.8745 | 0.9689 | 0.9418 | 0.9314 | 0.9786 | 0.9203 | 0.8904 | 0.9717 |
None | 0.0027 | 0.0002 | 0.0001 | 0 | 0.0027 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0008 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0139 | 0.0009 | 0.0001 | 0 | 0.0134 | 0.0015 | 0.0035 | 0.0001 | 0.001 | 0.0038 | 0.0016 | 0.0018 | 0.0003 | 0.0001 | 0 | 0 | 0.0078 | 0.0001 |
Nucleolus | 0.0067 | 0.0053 | 0.0003 | 0 | 0.009 | 0.0003 | 0.0008 | 0.0002 | 0.0001 | 0.0002 | 0.0011 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0 | 0.0002 | 0 |
Nucleus | 0.0111 | 0.0016 | 0.0001 | 0 | 0.0224 | 0.0005 | 0.0099 | 0.0001 | 0.0004 | 0.0006 | 0.0014 | 0.0032 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 |
Peroxisomes | 0.0035 | 0.0095 | 0.0077 | 0.0007 | 0.0051 | 0.0051 | 0.0114 | 0.0066 | 0.0023 | 0.0007 | 0.0072 | 0.0024 | 0.0031 | 0.0072 | 0.0026 | 0.0004 | 0.0023 | 0.0006 |
Punctate Nuclear | 0.002 | 0.0011 | 0.0001 | 0 | 0.0178 | 0.0007 | 0.0008 | 0 | 0.0002 | 0.0007 | 0.0038 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 | 0 |
Vacuole | 0.0151 | 0.0143 | 0.0012 | 0.0003 | 0.0032 | 0.0043 | 0.0014 | 0.0027 | 0.0009 | 0.0048 | 0.0024 | 0.0002 | 0.0017 | 0.0083 | 0.001 | 0.0008 | 0.0012 | 0.0004 |
Vacuole Periphery | 0.0151 | 0.0172 | 0.0045 | 0.0033 | 0.0056 | 0.0046 | 0.004 | 0.0083 | 0.003 | 0.0286 | 0.0114 | 0.0012 | 0.0075 | 0.0078 | 0.0035 | 0.0021 | 0.0042 | 0.0049 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.1902 | 17.3048 | 29.4586 | 34.6806 | 34.6949 | 42.1087 | 34.2219 | 39.5957 | 40.7933 | 41.4088 |
Translational Efficiency | 1.1923 | 1.5161 | 0.9505 | 0.997 | 0.7481 | 0.8968 | 1.1925 | 1.0045 | 1.0082 | 0.9232 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|