Standard name | |
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Human Ortholog | |
Description | Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 0 | 2 | 5 | 8 | 6 | 8 | 7 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 0 | 1 | 3 | 5 | 8 | 10 | 5 | 4 | 2 | 9 | 5 | 6 | 3 | 11 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 72 | – | 16 | 80 | 154 | 84 | 114 | 105 | 76 | 105 | 52 | 49 | 204 | 232 | 318 | 193 | 104 | 141 | 78 | 22 | 26 | 93 | 202 | 223 |
Endoplasmic Reticulum | 0 | – | 0 | 0 | 9 | 2 | 23 | 30 | 18 | 17 | 10 | 13 | 9 | 4 | 5 | 8 | 6 | 5 | 2 | 0 | 0 | 3 | 22 | 17 |
Endosome | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 6 | 5 | 5 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 2 |
Nucleus | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | – | 0 | 1 | 1 | 0 | 1 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 4 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 1 |
Unique Cell Count | 72 | 16 | 81 | 165 | 88 | 148 | 150 | 103 | 135 | 68 | 69 | 212 | 238 | 322 | 203 | 113 | 144 | 85 | 29 | 32 | 105 | 235 | 255 | |
Labelled Cell Count | 72 | 16 | 82 | 167 | 93 | 156 | 161 | 115 | 143 | 78 | 80 | 221 | 245 | 326 | 217 | 119 | 149 | 85 | 29 | 32 | 105 | 235 | 255 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 77.3 | – | 85.9 | 80.8 | 60.1 | 48.3 | 41.8 | 40.2 | 35.3 | 36.8 | 34.4 | 36.0 | 95.4 | 90.9 | 92.4 | 141.1 | 135.8 | 132.5 | 81.4 | 84.4 | 87.7 |
Std Deviation (1e-4) | 15.8 | – | 13.8 | 21.9 | 17.1 | 11.6 | 10.6 | 11.1 | 10.9 | 9.7 | 12.6 | 8.5 | 17.7 | 20.7 | 21.7 | 52.9 | 52.0 | 49.9 | 23.6 | 21.7 | 22.7 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.52 | -0.83 | -1.04 | -1.1 | -1.28 | -1.22 | -1.32 | -1.26 | 0.15 | 0.08 | 0.1 | 0.71 | 0.66 | 0.62 | -0.08 | -0.03 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | -1.1 | -0.9 | -2.2 | -2.6 | -2.3 | -2.1 | -2.2 | -2.5 | -0.8 | -0.6 | -0.4 | -0.9 | -1.2 | -0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0059 | 0.001 | 0.0014 | 0.001 | 0.0411 | 0.0043 | – | – | – | – | – | – | 0.0009 | 0.0003 | 0.0352 | 0.0009 | 0.0038 | 0.0253 |
Bud | 0.0031 | 0.001 | 0.0044 | 0.0024 | 0.0021 | 0.0039 | – | – | – | – | – | – | 0.0008 | 0.0006 | 0.0167 | 0.0035 | 0.0178 | 0.0054 |
Bud Neck | 0.0006 | 0.0005 | 0.0006 | 0.0012 | 0.0119 | 0.0026 | – | – | – | – | – | – | 0.0006 | 0.0002 | 0.0004 | 0.0009 | 0.0021 | 0.0016 |
Bud Periphery | 0.0046 | 0.0007 | 0.0063 | 0.0016 | 0.0037 | 0.0036 | – | – | – | – | – | – | 0.0003 | 0.0001 | 0.0229 | 0.0004 | 0.0036 | 0.0035 |
Bud Site | 0.0025 | 0.003 | 0.0023 | 0.0007 | 0.0111 | 0.0012 | – | – | – | – | – | – | 0.0002 | 0.0006 | 0.0025 | 0.0001 | 0.0036 | 0.0004 |
Cell Periphery | 0.0193 | 0.0271 | 0.0364 | 0.0085 | 0.0051 | 0.0064 | – | – | – | – | – | – | 0.0013 | 0.0011 | 0.0061 | 0.0006 | 0.0039 | 0.0012 |
Cytoplasm | 0.6742 | 0.8834 | 0.7402 | 0.8417 | 0.6711 | 0.6644 | – | – | – | – | – | – | 0.7843 | 0.9857 | 0.7373 | 0.9151 | 0.9088 | 0.7248 |
Cytoplasmic Foci | 0.017 | 0.0037 | 0.0051 | 0.0035 | 0.0052 | 0.0058 | – | – | – | – | – | – | 0.0017 | 0.0007 | 0.0018 | 0.0014 | 0.0009 | 0.007 |
Eisosomes | 0.0004 | 0.0003 | 0.0003 | 0 | 0.005 | 0.0008 | – | – | – | – | – | – | 0 | 0 | 0.0015 | 0 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0296 | 0.0106 | 0.0114 | 0.0229 | 0.0132 | 0.0163 | – | – | – | – | – | – | 0.0107 | 0.0003 | 0.001 | 0.0005 | 0.0008 | 0.0256 |
Endosome | 0.02 | 0.0026 | 0.018 | 0.0153 | 0.0899 | 0.036 | – | – | – | – | – | – | 0.0085 | 0.0006 | 0.0036 | 0.0019 | 0.001 | 0.024 |
Golgi | 0.0064 | 0.0012 | 0.0073 | 0.0007 | 0.0091 | 0.0207 | – | – | – | – | – | – | 0.0019 | 0.0001 | 0.0026 | 0.0001 | 0.0001 | 0.0139 |
Lipid Particles | 0.0098 | 0.0027 | 0.0034 | 0.001 | 0.0027 | 0.0021 | – | – | – | – | – | – | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0003 |
Mitochondria | 0.0103 | 0.0058 | 0.0456 | 0.001 | 0.006 | 0.0675 | – | – | – | – | – | – | 0.0039 | 0.0001 | 0.0986 | 0.0011 | 0.0009 | 0.1136 |
None | 0.0113 | 0.0018 | 0.0071 | 0.0021 | 0.0026 | 0.0018 | – | – | – | – | – | – | 0.0036 | 0.0001 | 0.0016 | 0.0007 | 0.0003 | 0.0021 |
Nuclear Periphery | 0.04 | 0.0041 | 0.0089 | 0.0052 | 0.0068 | 0.0328 | – | – | – | – | – | – | 0.0321 | 0.0005 | 0.005 | 0.0062 | 0.0071 | 0.0125 |
Nucleolus | 0.0133 | 0.0017 | 0.0038 | 0.0005 | 0.0028 | 0.0014 | – | – | – | – | – | – | 0.001 | 0.0001 | 0.0023 | 0.0003 | 0.0005 | 0.0001 |
Nucleus | 0.0257 | 0.0033 | 0.0107 | 0.0044 | 0.0088 | 0.0145 | – | – | – | – | – | – | 0.1196 | 0.0008 | 0.0091 | 0.0076 | 0.0171 | 0.0051 |
Peroxisomes | 0.002 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0005 | – | – | – | – | – | – | 0 | 0 | 0.0008 | 0.0001 | 0.0002 | 0.0004 |
Punctate Nuclear | 0.0033 | 0.0003 | 0.0004 | 0.0001 | 0.0006 | 0.0005 | – | – | – | – | – | – | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0004 |
Vacuole | 0.0749 | 0.0411 | 0.0711 | 0.0678 | 0.0816 | 0.0691 | – | – | – | – | – | – | 0.0184 | 0.007 | 0.0353 | 0.0362 | 0.0213 | 0.0176 |
Vacuole Periphery | 0.0256 | 0.004 | 0.0151 | 0.0184 | 0.0191 | 0.0438 | – | – | – | – | – | – | 0.0097 | 0.001 | 0.0151 | 0.022 | 0.0058 | 0.015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2097.7367 | 2047.5809 | 2269.8357 | 2095.5414 | 2399.1529 | 1837.9893 | 1811.232 | 1623.2405 | 1909.8991 | 1769.0447 |
Translational Efficiency | 1.5351 | 1.6241 | 1.5303 | 1.5174 | 1.5375 | 1.7672 | 1.5523 | 1.8156 | 1.5369 | 1.776 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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