Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.05 0.06 0.06 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.06 0.05 0.07 0.05 0 0 0.13 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.99 0.93 0.95 0.77 0.7 0.74 0.78 0.76 0.71 0.96 0.97 0.99 0.95 0.92 0.98 0.92 0.77 0.83 0.89 0.86 0.88
Endoplasmic Reticulum 0 0 0 0.05 0 0.16 0.2 0.17 0.13 0.15 0.19 0 0 0 0 0.05 0 0 0 0 0 0.09 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 5 8 6 8 7 6 1 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 3 5 8 10 5 4 2 9 5 6 3 11 6 1 0 0 0 0 0 0
Cytoplasm 72 16 80 154 84 114 105 76 105 52 49 204 232 318 193 104 141 78 22 26 93 202 223
Endoplasmic Reticulum 0 0 0 9 2 23 30 18 17 10 13 9 4 5 8 6 5 2 0 0 3 22 17
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 3 2 1 1 1 1 1 2 0 0 0 1 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 1 2 6 5 5 1 1 0 0 1 1 0 0 1 0 0 2 2
Nucleus 0 0 0 0 0 0 0 2 1 1 1 0 0 0 0 0 0 1 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 0 1 4 0 2 0 0 0 1 0 4 2 1 0 1 1 0 0 1
Unique Cell Count 72 16 81 165 88 148 150 103 135 68 69 212 238 322 203 113 144 85 29 32 105 235 255
Labelled Cell Count 72 16 82 167 93 156 161 115 143 78 80 221 245 326 217 119 149 85 29 32 105 235 255


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 77.3 85.9 80.8 60.1 48.3 41.8 40.2 35.3 36.8 34.4 36.0 95.4 90.9 92.4 141.1 135.8 132.5 81.4 84.4 87.7
Std Deviation (1e-4) 15.8 13.8 21.9 17.1 11.6 10.6 11.1 10.9 9.7 12.6 8.5 17.7 20.7 21.7 52.9 52.0 49.9 23.6 21.7 22.7
Intensity Change (Log2) -0.09 -0.52 -0.83 -1.04 -1.1 -1.28 -1.22 -1.32 -1.26 0.15 0.08 0.1 0.71 0.66 0.62 -0.08 -0.03 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -1.1 -0.9 -2.2 -2.6 -2.3 -2.1 -2.2 -2.5 -0.8 -0.6 -0.4 -0.9 -1.2 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0059 0.001 0.0014 0.001 0.0411 0.0043 0.0009 0.0003 0.0352 0.0009 0.0038 0.0253
Bud 0.0031 0.001 0.0044 0.0024 0.0021 0.0039 0.0008 0.0006 0.0167 0.0035 0.0178 0.0054
Bud Neck 0.0006 0.0005 0.0006 0.0012 0.0119 0.0026 0.0006 0.0002 0.0004 0.0009 0.0021 0.0016
Bud Periphery 0.0046 0.0007 0.0063 0.0016 0.0037 0.0036 0.0003 0.0001 0.0229 0.0004 0.0036 0.0035
Bud Site 0.0025 0.003 0.0023 0.0007 0.0111 0.0012 0.0002 0.0006 0.0025 0.0001 0.0036 0.0004
Cell Periphery 0.0193 0.0271 0.0364 0.0085 0.0051 0.0064 0.0013 0.0011 0.0061 0.0006 0.0039 0.0012
Cytoplasm 0.6742 0.8834 0.7402 0.8417 0.6711 0.6644 0.7843 0.9857 0.7373 0.9151 0.9088 0.7248
Cytoplasmic Foci 0.017 0.0037 0.0051 0.0035 0.0052 0.0058 0.0017 0.0007 0.0018 0.0014 0.0009 0.007
Eisosomes 0.0004 0.0003 0.0003 0 0.005 0.0008 0 0 0.0015 0 0 0.0002
Endoplasmic Reticulum 0.0296 0.0106 0.0114 0.0229 0.0132 0.0163 0.0107 0.0003 0.001 0.0005 0.0008 0.0256
Endosome 0.02 0.0026 0.018 0.0153 0.0899 0.036 0.0085 0.0006 0.0036 0.0019 0.001 0.024
Golgi 0.0064 0.0012 0.0073 0.0007 0.0091 0.0207 0.0019 0.0001 0.0026 0.0001 0.0001 0.0139
Lipid Particles 0.0098 0.0027 0.0034 0.001 0.0027 0.0021 0.0001 0 0.0005 0.0001 0.0001 0.0003
Mitochondria 0.0103 0.0058 0.0456 0.001 0.006 0.0675 0.0039 0.0001 0.0986 0.0011 0.0009 0.1136
None 0.0113 0.0018 0.0071 0.0021 0.0026 0.0018 0.0036 0.0001 0.0016 0.0007 0.0003 0.0021
Nuclear Periphery 0.04 0.0041 0.0089 0.0052 0.0068 0.0328 0.0321 0.0005 0.005 0.0062 0.0071 0.0125
Nucleolus 0.0133 0.0017 0.0038 0.0005 0.0028 0.0014 0.001 0.0001 0.0023 0.0003 0.0005 0.0001
Nucleus 0.0257 0.0033 0.0107 0.0044 0.0088 0.0145 0.1196 0.0008 0.0091 0.0076 0.0171 0.0051
Peroxisomes 0.002 0.0002 0.0003 0.0001 0.0005 0.0005 0 0 0.0008 0.0001 0.0002 0.0004
Punctate Nuclear 0.0033 0.0003 0.0004 0.0001 0.0006 0.0005 0.0002 0 0.0001 0.0001 0.0001 0.0004
Vacuole 0.0749 0.0411 0.0711 0.0678 0.0816 0.0691 0.0184 0.007 0.0353 0.0362 0.0213 0.0176
Vacuole Periphery 0.0256 0.004 0.0151 0.0184 0.0191 0.0438 0.0097 0.001 0.0151 0.022 0.0058 0.015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2097.7367 2047.5809 2269.8357 2095.5414 2399.1529 1837.9893 1811.232 1623.2405 1909.8991 1769.0447
Translational Efficiency 1.5351 1.6241 1.5303 1.5174 1.5375 1.7672 1.5523 1.8156 1.5369 1.776

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps19b

Rps19b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps19b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available