Standard name | |
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Human Ortholog | |
Description | Molecular chaperone; required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; N-terminally propionylated in vivo |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | – | 5 | 15 | 5 | 5 | 3 | 0 | 1 | 1 | 1 | 38 | 30 | 28 | 2 | 5 | 11 | 5 | 4 | 1 | 11 | 31 | 28 |
Bud | 4 | 0 | – | 0 | 2 | 4 | 8 | 5 | 1 | 0 | 4 | 3 | 3 | 3 | 4 | 1 | 6 | 3 | 0 | 1 | 0 | 3 | 5 | 6 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 2 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | – | 6 | 9 | 4 | 2 | 0 | 0 | 1 | 2 | 0 | 19 | 26 | 26 | 16 | 14 | 14 | 0 | 0 | 0 | 0 | 0 | 2 |
Cytoplasm | 6 | 4 | – | 1 | 9 | 11 | 8 | 9 | 5 | 6 | 2 | 3 | 13 | 23 | 26 | 12 | 15 | 18 | 0 | 1 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 4 | 3 | – | 2 | 8 | 4 | 1 | 0 | 1 | 3 | 1 | 1 | 9 | 12 | 21 | 2 | 6 | 3 | 1 | 1 | 0 | 0 | 4 | 5 |
Endosome | 11 | 3 | – | 3 | 13 | 8 | 8 | 8 | 3 | 2 | 0 | 3 | 18 | 32 | 47 | 15 | 16 | 12 | 25 | 6 | 2 | 20 | 44 | 41 |
Golgi | 17 | 23 | – | 44 | 98 | 27 | 22 | 22 | 4 | 5 | 4 | 4 | 236 | 222 | 220 | 27 | 37 | 39 | 26 | 30 | 4 | 42 | 81 | 91 |
Mitochondria | 225 | 81 | – | 59 | 61 | 101 | 201 | 113 | 81 | 116 | 150 | 102 | 94 | 62 | 40 | 23 | 15 | 8 | 173 | 62 | 34 | 134 | 222 | 275 |
Nucleus | 2 | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 1 | 1 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 12 | 2 | – | 1 | 2 | 8 | 14 | 9 | 5 | 4 | 9 | 5 | 0 | 7 | 4 | 0 | 2 | 1 | 7 | 1 | 1 | 4 | 7 | 7 |
Nucleolus | 18 | 4 | – | 0 | 5 | 8 | 20 | 18 | 15 | 23 | 15 | 17 | 2 | 2 | 1 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 2 | 1 |
Peroxisomes | 0 | 0 | – | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 4 | 4 | 5 | 2 | 12 | 3 | 2 | 14 | 29 | 26 |
SpindlePole | 3 | 0 | – | 0 | 0 | 0 | 3 | 3 | 2 | 3 | 0 | 1 | 3 | 8 | 1 | 8 | 3 | 3 | 1 | 0 | 0 | 3 | 7 | 7 |
Vac/Vac Membrane | 50 | 14 | – | 8 | 37 | 33 | 31 | 13 | 4 | 19 | 9 | 10 | 82 | 101 | 104 | 39 | 20 | 12 | 5 | 8 | 1 | 9 | 15 | 16 |
Unique Cell Count | 261 | 104 | 82 | 174 | 164 | 252 | 157 | 100 | 157 | 162 | 124 | 355 | 383 | 419 | 111 | 110 | 103 | 269 | 126 | 50 | 250 | 463 | 522 | |
Labelled Cell Count | 353 | 138 | 130 | 260 | 215 | 327 | 204 | 124 | 188 | 198 | 151 | 521 | 531 | 526 | 154 | 147 | 127 | 269 | 126 | 50 | 250 | 463 | 522 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 10.0 | – | 9.3 | 10.7 | 9.7 | 9.4 | 9.3 | 8.5 | 8.4 | 8.3 | 8.7 | 11.2 | 10.6 | 10.3 | 10.1 | 9.9 | 9.8 | 10.5 | 10.1 | 10.4 |
Std Deviation (1e-4) | 1.3 | 2.2 | – | 2.0 | 1.7 | 1.7 | 1.9 | 1.7 | 1.8 | 1.9 | 1.8 | 2.0 | 1.7 | 1.5 | 1.5 | 2.0 | 2.6 | 2.5 | 1.9 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2505 | 3.7565 | 3.801 | 3.1048 | 3.3753 | 3.7051 | 3.8474 | 4.6844 | 4.3624 | 3.8201 | 3.9341 | 4.0436 | 3.9793 | 4.09 | 4.0317 | 4.184 | 4.2125 | 4.1247 |
Actin | 0.0321 | 0.02 | 0.0237 | 0.0678 | 0.0536 | 0.0084 | 0.032 | 0.0188 | 0.0405 | 0.0226 | 0.0393 | 0.0162 | 0.0315 | 0.0334 | 0.0886 | 0.0127 | 0.0781 | 0.0267 |
Bud | 0.0009 | 0.0025 | 0.0011 | 0.0005 | 0.0003 | 0.0002 | 0.0022 | 0.0038 | 0.0045 | 0.0054 | 0.0021 | 0.0072 | 0.0007 | 0.0026 | 0.0025 | 0.0035 | 0.0157 | 0.0045 |
Bud Neck | 0.002 | 0.0008 | 0.0093 | 0.0062 | 0.0007 | 0.0006 | 0.0014 | 0.0008 | 0.0029 | 0.0008 | 0.002 | 0.0024 | 0.0009 | 0.0007 | 0.0013 | 0.0012 | 0.0007 | 0.0009 |
Bud Periphery | 0.0019 | 0.0028 | 0.0017 | 0.0014 | 0.0004 | 0.0004 | 0.0037 | 0.0042 | 0.006 | 0.0106 | 0.0045 | 0.0087 | 0.0014 | 0.0032 | 0.003 | 0.0065 | 0.027 | 0.002 |
Bud Site | 0.0065 | 0.0176 | 0.0087 | 0.0007 | 0.0014 | 0.0002 | 0.0107 | 0.0314 | 0.0144 | 0.0127 | 0.0038 | 0.0046 | 0.0018 | 0.018 | 0.0086 | 0.0066 | 0.0096 | 0.0009 |
Cell Periphery | 0.0006 | 0.0008 | 0.0008 | 0.0003 | 0.0001 | 0.0001 | 0.0011 | 0.0009 | 0.0014 | 0.0013 | 0.0005 | 0.001 | 0.0004 | 0.0008 | 0.001 | 0.0006 | 0.001 | 0.0004 |
Cytoplasm | 0.0063 | 0.0139 | 0.0197 | 0.0013 | 0.0081 | 0.0061 | 0.0189 | 0.0198 | 0.0234 | 0.0519 | 0.0224 | 0.0279 | 0.0029 | 0.0246 | 0.0094 | 0.0016 | 0.0041 | 0.0068 |
Cytoplasmic Foci | 0.0337 | 0.0349 | 0.0313 | 0.0222 | 0.0359 | 0.0065 | 0.0648 | 0.0431 | 0.0416 | 0.0174 | 0.0511 | 0.0397 | 0.0397 | 0.0321 | 0.0234 | 0.0321 | 0.0185 | 0.0232 |
Eisosomes | 0.0007 | 0.0003 | 0.0006 | 0.0007 | 0.0003 | 0.0002 | 0.0012 | 0.0005 | 0.0009 | 0.0006 | 0.0003 | 0.0003 | 0.0007 | 0.0004 | 0.0016 | 0.0004 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.004 | 0.0028 | 0.0024 | 0.0013 | 0.0047 | 0.0057 | 0.0059 | 0.0072 | 0.0061 | 0.0014 | 0.0038 | 0.0012 | 0.0026 | 0.002 | 0.002 | 0.0005 | 0.0019 | 0.0038 |
Endosome | 0.0915 | 0.1257 | 0.0769 | 0.047 | 0.1116 | 0.0439 | 0.1298 | 0.1006 | 0.0852 | 0.0486 | 0.161 | 0.0955 | 0.1102 | 0.0848 | 0.0681 | 0.0323 | 0.1234 | 0.0789 |
Golgi | 0.0732 | 0.1215 | 0.0779 | 0.0594 | 0.1272 | 0.0763 | 0.0696 | 0.1241 | 0.0892 | 0.046 | 0.1812 | 0.1008 | 0.1567 | 0.1532 | 0.1492 | 0.0899 | 0.1313 | 0.1538 |
Lipid Particles | 0.0653 | 0.0109 | 0.0124 | 0.0081 | 0.0441 | 0.0023 | 0.0464 | 0.0164 | 0.0093 | 0.0066 | 0.0139 | 0.0108 | 0.0294 | 0.011 | 0.005 | 0.0022 | 0.0011 | 0.0066 |
Mitochondria | 0.5427 | 0.5933 | 0.6826 | 0.7592 | 0.434 | 0.8419 | 0.4576 | 0.574 | 0.6225 | 0.7436 | 0.484 | 0.6498 | 0.5393 | 0.5969 | 0.5932 | 0.7896 | 0.5747 | 0.672 |
None | 0.0205 | 0.0008 | 0.0107 | 0.0004 | 0.0026 | 0.0003 | 0.0204 | 0.0085 | 0.0034 | 0.0005 | 0.0012 | 0.005 | 0.0247 | 0.0053 | 0.005 | 0.0003 | 0.0003 | 0.0053 |
Nuclear Periphery | 0.0258 | 0.0018 | 0.0014 | 0.001 | 0.0906 | 0.0008 | 0.0071 | 0.0012 | 0.006 | 0.0007 | 0.0008 | 0.0005 | 0.0104 | 0.0009 | 0.0049 | 0.0001 | 0.0006 | 0.0016 |
Nucleolus | 0.0088 | 0.0009 | 0.0003 | 0.0002 | 0.0011 | 0 | 0.0025 | 0.0007 | 0.0006 | 0.0003 | 0.0001 | 0.0011 | 0.0002 | 0.0004 | 0.0011 | 0.0002 | 0.0001 | 0.0003 |
Nucleus | 0.0129 | 0.0009 | 0.0006 | 0.0005 | 0.0133 | 0.0004 | 0.008 | 0.001 | 0.0008 | 0.0006 | 0.0004 | 0.0015 | 0.0005 | 0.0011 | 0.0119 | 0.0002 | 0.0004 | 0.0004 |
Peroxisomes | 0.0367 | 0.017 | 0.0277 | 0.0098 | 0.035 | 0.0015 | 0.0696 | 0.0284 | 0.0209 | 0.01 | 0.0177 | 0.011 | 0.025 | 0.0115 | 0.0071 | 0.0127 | 0.0029 | 0.0045 |
Punctate Nuclear | 0.0169 | 0.0004 | 0.0017 | 0.0002 | 0.0293 | 0.0003 | 0.0221 | 0.0004 | 0.0106 | 0.0002 | 0.0007 | 0.0015 | 0.0004 | 0.0004 | 0.0008 | 0.0008 | 0.0001 | 0.0002 |
Vacuole | 0.0093 | 0.0179 | 0.0049 | 0.0052 | 0.0018 | 0.0015 | 0.0168 | 0.01 | 0.0056 | 0.0137 | 0.0037 | 0.0074 | 0.0054 | 0.0106 | 0.0084 | 0.0027 | 0.0046 | 0.0028 |
Vacuole Periphery | 0.008 | 0.0126 | 0.0036 | 0.0064 | 0.0038 | 0.0024 | 0.0083 | 0.0041 | 0.0042 | 0.0047 | 0.0056 | 0.0058 | 0.0151 | 0.0058 | 0.004 | 0.0034 | 0.0038 | 0.0043 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.9776 | 14.4297 | 14.5316 | 25.482 | 26.1606 | 31.1133 | 41.1644 | 40.7955 | 38.184 | 37.3307 |
Translational Efficiency | 1.1198 | 1.8546 | 1.9411 | 1.0111 | 1.0453 | 1.5327 | 1.0082 | 0.9665 | 1.2674 | 1.1205 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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