Standard name
Human Ortholog
Description Putative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.97 0.95 0.85 0.77 0.79 0.76 0.73 0.62 0.64 0.98 0.99 0.98 0.93 0.9 0.88 0.95 0.93 0.89 0.95 0.97 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.23 0.16 0.23 0.21 0.41 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0.05 0.07 0.09 0.07 0.09 0.05 0.13 0.1 0.19 0 0 0 0.09 0.13 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3 2 3 8 10 1 0 1 0 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 2 2 3 0 1 0 0 0 2 1 3 8 5 8 0 0 0 0 0 0
Cytoplasm 191 204 140 167 312 221 231 282 185 201 183 173 382 576 544 136 144 128 179 191 143 175 382 459
Endoplasmic Reticulum 1 2 0 0 1 3 3 0 4 0 1 0 1 0 2 2 2 3 0 0 1 0 1 1
Endosome 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 2 3 0 0 0 0 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 0 0 0 0
Mitochondria 3 2 0 2 3 28 68 57 56 59 121 78 0 0 0 0 4 2 0 3 2 0 1 0
Nucleus 0 1 1 5 2 4 12 10 13 9 17 16 3 5 2 1 0 3 0 5 2 1 0 2
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 7 8 22 24 22 33 12 37 31 51 10 12 18 13 21 10 0 1 2 1 1 3
Unique Cell Count 193 206 144 172 327 259 299 359 244 277 296 269 389 582 555 146 160 145 189 206 160 185 395 477
Labelled Cell Count 195 209 148 183 342 282 342 387 275 316 364 319 398 595 569 162 178 158 189 206 160 185 395 477


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 8.7 7.6 7.3 7.3 6.1 5.6 6.1 5.3 5.8 5.0 5.4 7.6 8.0 8.0 12.1 11.9 11.9 7.8 8.5 8.9
Std Deviation (1e-4) 0.7 1.1 1.0 0.8 1.7 1.3 1.2 1.2 1.4 1.5 1.1 0.8 0.9 0.9 0.9 2.3 1.9 2.2 1.2 1.3 1.4
Intensity Change (Log2) – – – -0.05 -0.06 -0.31 -0.44 -0.33 -0.52 -0.39 -0.6 -0.49 0.0 0.08 0.08 0.67 0.64 0.64 0.03 0.16 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -0.9 -3.7 -5.3 -5.1 -5.5 -6.1 -7.9 -7.4 0.7 1.6 0.6 -1.6 -2.5 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 6.2 5.1 6.2 6.0 9.0 7.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 2.4 0 2.5 2.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.1 0.8 1.6 1.0 1.6 0 2.7 2.0 3.9 0 0 -0.9 1.4 2.5 0.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2027 1.9543 1.7884 1.4478 1.1279 1.6921 2.2925 2.9235 2.5722 1.6498 2.7579 2.4716 2.1076 2.7938 2.4006 2.5615 2.4073 2.1161
Actin 0.014 0.0011 0.0104 0.0138 0.0015 0.008 0.023 0.0012 0.0139 0.0012 0.0523 0.0084 0.0319 0.0009 0.0335 0.0364 0.0437 0.0044
Bud 0.0006 0.0004 0.0004 0.0005 0.0026 0.0006 0.002 0.0037 0.0031 0.0001 0.0036 0.0006 0.0044 0.0005 0.0044 0.0063 0.0348 0.0054
Bud Neck 0.002 0.0004 0.0009 0.0007 0.0007 0.0024 0.0097 0.0004 0.0012 0.0005 0.0017 0.0149 0.0017 0.0004 0.005 0.0014 0.0012 0.0013
Bud Periphery 0.0006 0.0002 0.0011 0.0003 0.0012 0.0006 0.0019 0.0011 0.0022 0 0.0043 0.0004 0.005 0.0004 0.0015 0.0038 0.0114 0.0021
Bud Site 0.0028 0.0017 0.0007 0.0008 0.0056 0.0006 0.0085 0.0057 0.0086 0.0003 0.0047 0.0003 0.0041 0.004 0.0083 0.0119 0.0041 0.0003
Cell Periphery 0.0001 0 0.0001 0 0.0001 0 0.0004 0.0001 0.0002 0.0001 0.0002 0 0.0012 0.0002 0.0005 0.0005 0.0005 0.0002
Cytoplasm 0.6262 0.7955 0.7519 0.7192 0.667 0.781 0.6033 0.8385 0.8172 0.8446 0.544 0.8122 0.7362 0.8117 0.7424 0.6733 0.6887 0.8193
Cytoplasmic Foci 0.0292 0.0101 0.0122 0.0144 0.0122 0.0253 0.0546 0.005 0.0115 0.0064 0.0413 0.0167 0.0223 0.003 0.0151 0.0469 0.0142 0.0204
Eisosomes 0.0002 0 0.0001 0 0 0.0001 0.0002 0 0.0001 0 0.0004 0 0.0002 0 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0046 0.0027 0.0031 0.0028 0.0028 0.0038 0.0067 0.0024 0.0025 0.0047 0.0055 0.0034 0.0084 0.0048 0.0092 0.0071 0.0164 0.0036
Endosome 0.0217 0.0058 0.0058 0.0052 0.005 0.0137 0.0381 0.0012 0.0024 0.0032 0.0354 0.0099 0.0134 0.0011 0.014 0.0192 0.0157 0.0113
Golgi 0.0051 0.0003 0.0013 0.0012 0.0003 0.0014 0.009 0.0002 0.0007 0.0003 0.0188 0.0015 0.0015 0.0001 0.0057 0.0094 0.0068 0.0022
Lipid Particles 0.0083 0.0001 0.001 0.0003 0.0002 0.001 0.0108 0.0001 0.0005 0.0003 0.0467 0.0011 0.0008 0.0001 0.0012 0.0055 0.0041 0.001
Mitochondria 0.0079 0.0005 0.0028 0.0011 0.0005 0.0017 0.0092 0.0003 0.0008 0.0003 0.0053 0.0006 0.0021 0.0002 0.0037 0.0062 0.0037 0.0035
None 0.2286 0.1415 0.168 0.1898 0.235 0.1095 0.1577 0.1195 0.1106 0.1176 0.1207 0.0889 0.1261 0.1652 0.1219 0.0976 0.0756 0.1023
Nuclear Periphery 0.0058 0.0035 0.0041 0.0035 0.0037 0.0049 0.0121 0.0013 0.0024 0.0023 0.0167 0.0035 0.0176 0.0016 0.0074 0.0137 0.0102 0.004
Nucleolus 0.0004 0.0002 0.0004 0.0003 0.0009 0.0004 0.0014 0.0002 0.0005 0.0001 0.0019 0.0002 0.0013 0.0001 0.0005 0.0009 0.0021 0.0009
Nucleus 0.0121 0.0294 0.0228 0.0234 0.0453 0.0257 0.0079 0.0137 0.0119 0.015 0.0066 0.0225 0.0113 0.0037 0.0099 0.0142 0.0258 0.012
Peroxisomes 0.0215 0.0004 0.0035 0.0162 0.0006 0.0034 0.0226 0.0003 0.0018 0.0002 0.0219 0.001 0.0005 0.0001 0.0013 0.0105 0.0014 0.0003
Punctate Nuclear 0.0058 0.0041 0.0066 0.0032 0.0133 0.0142 0.0134 0.0042 0.007 0.002 0.0653 0.0127 0.0054 0.0011 0.012 0.0297 0.0356 0.003
Vacuole 0.0019 0.0017 0.0019 0.0029 0.0014 0.0014 0.0046 0.0009 0.0008 0.0007 0.0015 0.0008 0.0024 0.0008 0.0019 0.0038 0.0025 0.0016
Vacuole Periphery 0.0006 0.0003 0.0008 0.0004 0.0003 0.0004 0.0031 0.0001 0.0002 0.0001 0.0012 0.0002 0.0022 0.0001 0.0006 0.0014 0.0011 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.1803 11.3733 19.1807 21.1459 21.7441 21.9634 16.4653 22.2259 21.5461 21.8908
Translational Efficiency 0.6352 1.041 0.6154 0.4937 0.64 0.9084 0.968 0.5566 0.578 0.6826

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bud17

Bud17


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available