Standard name | |
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Human Ortholog | |
Description | RNase of the DEDD superfamily; subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 2 | 2 | 3 | 8 | 6 | 7 | 4 | 4 | 10 | 3 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 11 | 9 | 4 | 7 | 17 | 24 | 28 | 25 | 14 | 9 | 2 | 17 | 7 | 10 | 11 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 147 | 231 | 202 | 173 | 172 | 199 | 247 | 181 | 202 | 174 | 121 | 123 | 329 | 417 | 424 | 121 | 134 | 106 | 148 | 219 | 201 | 293 | 394 | 343 |
Endoplasmic Reticulum | 0 | 1 | 23 | 13 | 12 | 12 | 28 | 33 | 41 | 31 | 25 | 25 | 8 | 12 | 2 | 5 | 4 | 1 | 2 | 4 | 19 | 5 | 7 | 16 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 |
Golgi | 0 | 1 | 0 | 1 | 2 | 4 | 4 | 5 | 4 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 14 | 0 | 1 | 5 | 13 | 78 | 125 | 77 | 94 | 139 | 75 | 92 | 0 | 0 | 1 | 3 | 5 | 2 | 1 | 0 | 1 | 1 | 4 | 4 |
Nucleus | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 1 | 4 | 5 | 5 | 8 | 21 | 9 | 10 | 8 | 4 | 0 | 3 | 5 | 0 | 11 | 6 | 2 | 1 | 1 | 2 | 2 | 2 |
Unique Cell Count | 153 | 233 | 222 | 188 | 198 | 268 | 370 | 294 | 319 | 322 | 206 | 232 | 336 | 428 | 433 | 131 | 152 | 116 | 160 | 232 | 229 | 309 | 426 | 380 |
Labelled Cell Count | 163 | 235 | 242 | 209 | 212 | 313 | 437 | 354 | 384 | 390 | 257 | 263 | 341 | 454 | 446 | 142 | 166 | 128 | 160 | 232 | 229 | 309 | 426 | 380 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.4 | 14.6 | 12.8 | 12.3 | 11.6 | 10.5 | 10.7 | 10.7 | 10.7 | 10.3 | 10.5 | 10.0 | 12.8 | 12.4 | 12.1 | 14.8 | 13.9 | 15.0 | 13.1 | 13.8 | 14.6 |
Std Deviation (1e-4) | 1.5 | 1.8 | 1.6 | 1.6 | 2.0 | 1.9 | 2.1 | 2.2 | 2.4 | 2.4 | 2.5 | 2.6 | 1.7 | 1.6 | 1.3 | 3.5 | 2.6 | 3.4 | 1.9 | 2.1 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.14 | -0.28 | -0.26 | -0.26 | -0.26 | -0.31 | -0.28 | -0.35 | 0.0 | -0.05 | -0.08 | 0.21 | 0.13 | 0.23 | 0.04 | 0.1 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.1 | -1.6 | -1.4 | -0.2 | 1.4 | 1.7 | 1.3 | 0.8 | -0.6 | -3.3 | -0.6 | -2.5 | 1.0 | 0.9 | 1.6 |
Cytoplasm | 0.4 | -1.4 | -4.8 | -6.7 | -7.6 | -7.3 | -9.2 | -7.7 | -9.0 | 3.7 | 3.7 | 4.1 | 0.4 | -0.9 | 0.1 |
Endoplasmic Reticulum | -1.2 | -1.6 | -2.5 | -1.2 | 0.3 | 0.9 | -0.3 | 0.6 | 0.1 | -4.0 | -4.0 | -6.3 | -2.2 | -2.8 | -3.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 3.5 | 8.6 | 9.6 | 8.1 | 8.7 | 11.2 | 9.7 | 10.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.1583 | 10.4764 | 10.0815 | 9.2717 | 9.5248 | 9.9534 | 6.4531 | 9.784 | 8.8303 | 8.495 | 6.5995 | 8.9828 | 8.6954 | 10.8616 | 9.7049 | 9.3704 | 9.2326 | 9.6205 |
Actin | 0.003 | 0.001 | 0.0049 | 0.0006 | 0.0011 | 0.0019 | 0.0452 | 0.0018 | 0.0114 | 0.0005 | 0.0193 | 0.001 | 0.0171 | 0.001 | 0.0106 | 0.0052 | 0.0028 | 0.0005 |
Bud | 0.0005 | 0.0003 | 0.0006 | 0.0005 | 0.0003 | 0.0005 | 0.001 | 0.0027 | 0.0014 | 0.0004 | 0.0055 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0045 | 0.0016 | 0.0003 |
Bud Neck | 0.0004 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.0013 | 0.0022 | 0.0003 | 0.0005 | 0.0005 | 0.0018 | 0.0012 | 0.0008 | 0.0002 | 0.0009 | 0.0008 | 0.0007 | 0.001 |
Bud Periphery | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0011 | 0.0012 | 0.0009 | 0.0001 | 0.0058 | 0.0004 | 0.0004 | 0.0001 | 0.0003 | 0.0014 | 0.0014 | 0.0002 |
Bud Site | 0.0016 | 0.0011 | 0.0011 | 0.0003 | 0.0002 | 0.0002 | 0.0071 | 0.0036 | 0.0015 | 0.0002 | 0.0246 | 0.0002 | 0.001 | 0.0005 | 0.0009 | 0.0017 | 0.0003 | 0.0001 |
Cell Periphery | 0.0013 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.6803 | 0.9266 | 0.8646 | 0.8229 | 0.8125 | 0.8044 | 0.634 | 0.9023 | 0.8314 | 0.8336 | 0.5193 | 0.8049 | 0.7682 | 0.935 | 0.8593 | 0.8039 | 0.7646 | 0.846 |
Cytoplasmic Foci | 0.0127 | 0.009 | 0.012 | 0.0142 | 0.034 | 0.0197 | 0.0283 | 0.0181 | 0.0324 | 0.0177 | 0.1148 | 0.0235 | 0.017 | 0.0098 | 0.0158 | 0.0193 | 0.0283 | 0.017 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.1838 | 0.0238 | 0.0534 | 0.0124 | 0.0197 | 0.0678 | 0.0802 | 0.0187 | 0.0228 | 0.0115 | 0.0093 | 0.0516 | 0.0921 | 0.024 | 0.0325 | 0.0387 | 0.0281 | 0.0538 |
Endosome | 0.0586 | 0.0239 | 0.0363 | 0.0941 | 0.1012 | 0.0692 | 0.0938 | 0.031 | 0.0553 | 0.1045 | 0.1258 | 0.0773 | 0.0626 | 0.0189 | 0.0565 | 0.0695 | 0.0844 | 0.0607 |
Golgi | 0.0128 | 0.003 | 0.0065 | 0.0038 | 0.0098 | 0.0124 | 0.0194 | 0.0052 | 0.0196 | 0.0042 | 0.0699 | 0.0102 | 0.01 | 0.0035 | 0.0056 | 0.0044 | 0.0144 | 0.0055 |
Lipid Particles | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0107 | 0.0003 | 0.0014 | 0.0001 | 0.0355 | 0.0002 | 0.0017 | 0.0001 | 0.0005 | 0.0003 | 0.0007 | 0.0001 |
Mitochondria | 0.0037 | 0.0004 | 0.0045 | 0.0011 | 0.001 | 0.0122 | 0.024 | 0.0037 | 0.0039 | 0.0012 | 0.0305 | 0.017 | 0.0049 | 0.0015 | 0.0018 | 0.0025 | 0.0311 | 0.0052 |
None | 0.0024 | 0.0014 | 0.001 | 0.0006 | 0.0008 | 0.0009 | 0.0042 | 0.0023 | 0.0009 | 0.0006 | 0.0018 | 0.0007 | 0.0026 | 0.0004 | 0.0007 | 0.0014 | 0.0009 | 0.0006 |
Nuclear Periphery | 0.0088 | 0.001 | 0.0031 | 0.0051 | 0.0016 | 0.0019 | 0.0169 | 0.001 | 0.0014 | 0.002 | 0.0027 | 0.0023 | 0.0043 | 0.0009 | 0.0026 | 0.0042 | 0.0223 | 0.0015 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 |
Nucleus | 0.002 | 0.0009 | 0.0012 | 0.0018 | 0.0009 | 0.001 | 0.0038 | 0.0009 | 0.0011 | 0.0012 | 0.0025 | 0.001 | 0.0018 | 0.0005 | 0.0012 | 0.0018 | 0.005 | 0.001 |
Peroxisomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0049 | 0.0004 | 0.0053 | 0.0001 | 0.0187 | 0.0004 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 |
Punctate Nuclear | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0053 | 0.0002 | 0.0003 | 0.0001 | 0.0031 | 0.0001 | 0.0012 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0001 |
Vacuole | 0.0219 | 0.0054 | 0.0072 | 0.0296 | 0.0128 | 0.0036 | 0.0121 | 0.0049 | 0.0058 | 0.0147 | 0.0056 | 0.005 | 0.0097 | 0.0023 | 0.0075 | 0.031 | 0.0056 | 0.0039 |
Vacuole Periphery | 0.0045 | 0.0011 | 0.0021 | 0.0118 | 0.0029 | 0.002 | 0.0044 | 0.001 | 0.0023 | 0.0066 | 0.0024 | 0.0024 | 0.0022 | 0.0007 | 0.002 | 0.0085 | 0.0065 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 33.0513 | 34.8489 | 36.5405 | 36.3557 | 31.8646 | 35.574 | 38.6827 | 37.4821 | 36.1621 | 39.9193 |
Translational Efficiency | 1.5712 | 1.6731 | 1.4484 | 1.2535 | 1.5183 | 1.5797 | 1.4691 | 1.3298 | 1.5554 | 1.2825 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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