Standard name | |
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Human Ortholog | |
Description | tRNA 2'-phosphotransferase that catalyzes final step in tRNA splicing: the transfer of the 2'-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1''-2''cyclic phosphate and nicotinamide |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3 |
Bud | 1 | 2 | 0 | 1 | 0 | 2 | 1 | – | 1 | 4 | 6 | 2 | 0 | 0 | 0 | – | 0 | 0 | 4 | 2 | 0 | 4 | 3 | 10 |
Bud Neck | 0 | 0 | 1 | 0 | 4 | 1 | 1 | – | 2 | 3 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 1 | 1 | 0 | 1 | 0 | – | 3 | 3 | 1 | 0 | 1 | 1 | 0 | 1 |
Cytoplasm | 205 | 212 | 33 | 16 | 101 | 93 | 160 | – | 115 | 122 | 104 | 172 | 175 | 281 | 314 | – | 88 | 92 | 188 | 190 | 54 | 160 | 180 | 230 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 1 | 0 | 1 | – | 1 | 0 | 0 | 2 | 0 | 1 | 0 | – | 7 | 6 | 0 | 1 | 0 | 3 | 2 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 2 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 2 |
Mitochondria | 7 | 4 | 0 | 0 | 1 | 15 | 12 | – | 63 | 61 | 85 | 117 | 0 | 1 | 1 | – | 2 | 6 | 12 | 5 | 2 | 4 | 9 | 4 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 1 | 0 | – | 3 | 1 | 4 | 3 | 0 | 0 | 0 | – | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 0 | 1 | 4 | 4 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 4 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 2 | 1 | 4 | – | 4 | 4 | 3 | 3 | 0 | 0 | 0 | – | 1 | 1 | 3 | 14 | 0 | 1 | 2 | 4 |
Unique Cell Count | 211 | 216 | 34 | 16 | 106 | 100 | 175 | 149 | 162 | 163 | 257 | 175 | 283 | 314 | 90 | 97 | 225 | 226 | 65 | 182 | 209 | 276 | ||
Labelled Cell Count | 215 | 221 | 35 | 17 | 112 | 114 | 182 | 190 | 197 | 209 | 307 | 175 | 285 | 315 | 101 | 111 | 225 | 226 | 65 | 182 | 209 | 276 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 4.2 | 4.8 | 3.7 | 5.5 | 3.8 | 4.6 | – | 3.9 | 4.1 | 3.8 | 3.8 | 4.7 | 4.9 | 4.9 | 4.9 | 5.2 | 5.1 | 4.7 | 5.3 | 4.8 |
Std Deviation (1e-4) | 1.3 | 0.9 | 0.7 | 0.2 | 2.2 | 1.7 | 1.8 | – | 1.8 | 1.7 | 1.6 | 1.4 | 1.6 | 1.2 | 1.1 | 0.8 | 1.1 | 1.2 | 1.6 | 9.5 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.38 | 0.18 | -0.34 | -0.09 | – | -0.3 | -0.24 | -0.35 | -0.35 | -0.03 | 0.01 | 0.02 | 0.01 | 0.1 | 0.09 | -0.05 | 0.14 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.0722 | -0.3496 | -0.8149 | -1.0129 | -1.0194 | -0.9428 | 0.8066 | 1.039 | 0.8556 | 0.6612 | 0.9583 | 0.8346 | 1.9962 | 2.8996 | 2.6389 | 2.709 | 2.3932 | 2.6403 |
Actin | 0.0232 | 0.001 | 0.0073 | 0.0422 | 0.006 | 0.0109 | 0.0262 | 0.0022 | 0.0071 | 0.0054 | 0.0002 | 0.008 | 0.0136 | 0.0004 | 0.0001 | 0.0061 | 0.0002 | 0.0003 |
Bud | 0.0005 | 0.0018 | 0.0001 | 0.0002 | 0.0002 | 0.0019 | 0.0026 | 0.0044 | 0.0041 | 0.0046 | 0.0001 | 0.0016 | 0.0003 | 0.0001 | 0 | 0.0003 | 0 | 0 |
Bud Neck | 0.0016 | 0.0001 | 0.0076 | 0.0004 | 0.0002 | 0.0008 | 0.0112 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0.002 | 0.0047 | 0 | 0 | 0.0012 | 0.0001 | 0.0002 |
Bud Periphery | 0.001 | 0.0017 | 0.0002 | 0.0003 | 0.0003 | 0.0031 | 0.0053 | 0.0045 | 0.0055 | 0.0265 | 0 | 0.0025 | 0.0006 | 0.0001 | 0 | 0.0004 | 0 | 0 |
Bud Site | 0.0022 | 0.0023 | 0.0009 | 0.0008 | 0.0009 | 0.001 | 0.0138 | 0.009 | 0.0093 | 0.0006 | 0.0001 | 0.0005 | 0.0016 | 0.0002 | 0.0001 | 0.0011 | 0 | 0 |
Cell Periphery | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 |
Cytoplasm | 0.1239 | 0.2056 | 0.1698 | 0.3057 | 0.1451 | 0.2226 | 0.2335 | 0.3723 | 0.4128 | 0.3786 | 0.3706 | 0.4485 | 0.1978 | 0.391 | 0.2932 | 0.2448 | 0.4685 | 0.4043 |
Cytoplasmic Foci | 0.0223 | 0.0092 | 0.009 | 0.009 | 0.0142 | 0.0178 | 0.0248 | 0.0105 | 0.0168 | 0.0086 | 0.0314 | 0.0204 | 0.0066 | 0.0045 | 0.0025 | 0.0046 | 0.0057 | 0.0051 |
Eisosomes | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0.0009 | 0.0017 | 0.002 | 0.0016 | 0.0015 | 0.0091 | 0.0011 | 0.0016 | 0.0011 | 0.0007 | 0.0014 | 0.0011 | 0.0012 | 0.0008 | 0.0028 | 0.0016 | 0.0008 |
Endosome | 0.0176 | 0.0021 | 0.0067 | 0.0061 | 0.0029 | 0.0067 | 0.0442 | 0.0079 | 0.0109 | 0.012 | 0.0295 | 0.0063 | 0.0025 | 0.0012 | 0.0004 | 0.0134 | 0.0015 | 0.0018 |
Golgi | 0.0045 | 0.0005 | 0.0016 | 0.0049 | 0.0049 | 0.0016 | 0.0107 | 0.0032 | 0.0039 | 0.0057 | 0.0042 | 0.0027 | 0.0011 | 0.0001 | 0 | 0.0035 | 0.0001 | 0.0002 |
Lipid Particles | 0.007 | 0.0003 | 0.0022 | 0.0058 | 0.0039 | 0.0025 | 0.0128 | 0.0009 | 0.0064 | 0.0006 | 0.0002 | 0.0029 | 0.0034 | 0.0002 | 0.0001 | 0.0017 | 0.0001 | 0.0001 |
Mitochondria | 0.0018 | 0.0196 | 0.0016 | 0.0024 | 0.0319 | 0.0028 | 0.0042 | 0.0023 | 0.0022 | 0.0375 | 0.0017 | 0.0048 | 0.0006 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0007 |
None | 0.7639 | 0.7503 | 0.7822 | 0.6038 | 0.756 | 0.7157 | 0.5461 | 0.568 | 0.5083 | 0.5111 | 0.4848 | 0.486 | 0.7556 | 0.5992 | 0.7013 | 0.7116 | 0.5205 | 0.5848 |
Nuclear Periphery | 0.0039 | 0.0003 | 0.0018 | 0.0017 | 0.0009 | 0.0018 | 0.0098 | 0.0009 | 0.0014 | 0.0009 | 0.0277 | 0.0018 | 0.0014 | 0.0002 | 0.0002 | 0.0023 | 0.0004 | 0.0002 |
Nucleolus | 0.001 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0054 | 0.0005 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0 | 0 | 0.0003 | 0 | 0 |
Nucleus | 0.003 | 0.001 | 0.0013 | 0.0011 | 0.0007 | 0.0012 | 0.0072 | 0.0019 | 0.0014 | 0.0012 | 0.0399 | 0.0024 | 0.0005 | 0.0006 | 0.0004 | 0.0014 | 0.0006 | 0.0006 |
Peroxisomes | 0.0107 | 0.0005 | 0.0021 | 0.0102 | 0.0278 | 0.0043 | 0.0109 | 0.0047 | 0.0022 | 0.0009 | 0.0057 | 0.0037 | 0.0041 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 |
Punctate Nuclear | 0.0064 | 0.0007 | 0.002 | 0.001 | 0.0008 | 0.0022 | 0.0063 | 0.0042 | 0.0012 | 0.0006 | 0.0007 | 0.0031 | 0.0031 | 0.0002 | 0.0002 | 0.0014 | 0.0003 | 0.0003 |
Vacuole | 0.0019 | 0.0011 | 0.0009 | 0.0014 | 0.0004 | 0.0006 | 0.0118 | 0.0008 | 0.0028 | 0.002 | 0.0008 | 0.0008 | 0.0004 | 0.0004 | 0.0003 | 0.0012 | 0.0003 | 0.0002 |
Vacuole Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0005 | 0.001 | 0.0003 | 0.0029 | 0.0002 | 0.0007 | 0.0016 | 0.0012 | 0.0002 | 0.0002 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.0871 | 7.1167 | 11.618 | 13.0887 | 11.3541 | 14.322 | 12.5925 | 12.1415 | 10.901 | 20.4007 |
Translational Efficiency | 1.5633 | 3.4138 | 1.416 | 1.2047 | 2.5623 | 2.6051 | 1.602 | 1.5521 | 1.8715 | 1.6418 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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