Standard name
Human Ortholog
Description mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth; human homolog EIF4E can complement yeast cdc33 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.08 0.05 – 0.05 0.08 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.98 0.95 0.91 0.88 – 0.81 0.81 0.85 0.76 1.0 0.98 0.97 0.99 0.99 1.0 0.96 0.94 0.97 0.95 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 – 0.14 0.14 0.08 0.16 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 2 3 5 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 0 – 1 1 1 2 0 0 0 0 0 0 0 2 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 0 3 12 12 – 11 22 11 32 2 2 7 0 2 0 0 0 0 0 0 0
Cytoplasm 190 202 112 113 148 136 227 – 190 224 156 222 208 312 361 68 91 92 171 194 103 221 313 380
Endoplasmic Reticulum 1 1 1 1 4 5 22 – 33 39 14 46 1 4 13 0 1 5 4 1 0 6 10 16
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 0 1 0 0 4 – 3 7 1 8 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 1 0 1 0 0 0 0 1 0 0 0 0 0 0 1 2
Nucleus 0 0 0 0 0 0 0 – 1 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 0 2 4 0 – 1 0 2 1 0 0 0 0 0 0 0 3 0 0 1 3
Unique Cell Count 192 205 113 115 155 150 257 235 278 184 293 208 317 371 69 92 92 180 208 107 234 330 410
Labelled Cell Count 194 206 113 115 158 159 265 244 296 192 316 211 318 381 69 94 97 180 208 107 234 330 410


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 101.5 87.6 81.8 78.3 78.4 54.4 63.6 – 53.4 53.9 49.8 53.7 101.1 91.4 103.1 96.2 99.8 104.0 92.6 100.2 106.3
Std Deviation (1e-4) 23.1 24.2 18.6 17.4 19.1 17.5 14.3 – 12.6 13.2 13.7 12.1 24.3 23.3 22.7 30.0 24.7 27.1 19.5 21.5 22.5
Intensity Change (Log2) – – – -0.06 -0.06 -0.59 -0.36 – -0.61 -0.6 -0.72 -0.61 0.31 0.16 0.33 0.23 0.29 0.35 0.18 0.29 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3.1 0 – 0 3.1 0 3.7 0 0 0 0 0 0
Cytoplasm -0.6 -1.7 -2.9 -3.4 – -4.7 -4.8 -4.0 -5.5 1.4 -0.5 -1.1 -0.4 -0.1 0.9
Endoplasmic Reticulum 0 0 0 2.8 – 3.9 3.9 2.6 4.2 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.1128 0.0007 0.0127 0.0008 0.0199 0.0259 0.2373 0.0003 0.001 0.0013 0.0176 0.0021 0.3675 0.0002 0.001 0.0095 0.0007 0.0102
Bud 0.0507 0.001 0.0438 0.0162 0.049 0.0145 0.0085 0.0004 0.0046 0.0017 0.0044 0.0025 0.0058 0.0002 0.0008 0.0004 0.0002 0.0013
Bud Neck 0.0441 0.0005 0.0013 0.0008 0.0063 0.0033 0.0015 0.0001 0.0003 0.0005 0.0005 0.001 0.0248 0.0001 0.0003 0.0004 0.0003 0.0005
Bud Periphery 0.101 0.0003 0.0674 0.0082 0.0118 0.0072 0.0054 0.0001 0.0024 0.0004 0.0083 0.0009 0.0059 0 0.0005 0.0001 0 0.0008
Bud Site 0.066 0.0017 0.0109 0.0016 0.0039 0.0007 0.0118 0.0002 0.0013 0.0001 0.0007 0.0014 0.0236 0.0001 0.0002 0.0002 0 0.0011
Cell Periphery 0.0749 0.005 0.0061 0.0038 0.0007 0.0011 0.0062 0.002 0.0006 0.0003 0.0005 0.0008 0.0022 0 0.0001 0.0001 0 0.0002
Cytoplasm 0.1206 0.8853 0.0215 0.2552 0.8059 0.8559 0.1987 0.9757 0.8936 0.8887 0.7495 0.7005 0.0979 0.991 0.9494 0.9249 0.9639 0.8758
Cytoplasmic Foci 0.008 0.0018 0.0072 0.0042 0.0236 0.0118 0.187 0.0023 0.0166 0.0053 0.0122 0.0195 0.0481 0.0019 0.0046 0.0049 0.0071 0.0205
Eisosomes 0.0009 0 0.0001 0 0 0 0.0012 0 0.0002 0 0.0001 0 0.0037 0 0 0 0 0.0004
Endoplasmic Reticulum 0.016 0.0015 0.0065 0.0027 0.0017 0.0032 0.0045 0.0021 0.0084 0.0327 0.0051 0.1013 0.0137 0.001 0.0043 0.0049 0.004 0.0024
Endosome 0.051 0.0024 0.1857 0.0646 0.0269 0.0093 0.0254 0.0015 0.017 0.0208 0.0438 0.1137 0.0489 0.0023 0.0101 0.0169 0.015 0.0125
Golgi 0.0173 0.0003 0.0619 0.0022 0.0055 0.0048 0.026 0.0003 0.0056 0.0021 0.0237 0.0163 0.0288 0.0002 0.0026 0.0031 0.0017 0.0036
Lipid Particles 0.0082 0.0008 0.0014 0.0006 0.0002 0 0.0368 0.0002 0.0035 0.0001 0.0008 0.0009 0.0293 0 0.0046 0.0006 0 0.0037
Mitochondria 0.011 0.0001 0.2525 0.0075 0.0149 0.0369 0.0214 0.0002 0.0203 0.0007 0.0302 0.0109 0.0709 0.0001 0.0093 0.0028 0.0009 0.0185
None 0.0022 0.0001 0.0009 0.0005 0.001 0.0012 0.0179 0.0015 0.0021 0.0005 0.0005 0.0007 0.0159 0.0001 0.0006 0.0002 0.0002 0.0067
Nuclear Periphery 0.0136 0.0012 0.0031 0.006 0.0011 0.0008 0.002 0.0005 0.0014 0.0139 0.0023 0.0024 0.0088 0.0002 0.0012 0.0016 0.0004 0.0006
Nucleolus 0.0048 0.0001 0.0008 0.0008 0.0001 0.0001 0.0055 0.0002 0.0019 0 0.0002 0.0001 0.0178 0 0.0002 0 0 0.0034
Nucleus 0.0131 0.0018 0.0028 0.0065 0.0029 0.0012 0.0085 0.0064 0.0012 0.0021 0.002 0.0015 0.0195 0.0003 0.0011 0.0008 0.0005 0.0015
Peroxisomes 0.0012 0 0.001 0.0004 0.0036 0.0028 0.0392 0 0.0012 0.0001 0.0005 0.0004 0.0791 0 0.001 0.0062 0 0.0295
Punctate Nuclear 0.0007 0 0.0001 0.0001 0.0002 0 0.0018 0.0001 0.0002 0.0001 0.0002 0.0003 0.019 0 0.0005 0 0 0.0026
Vacuole 0.2451 0.0885 0.1645 0.4104 0.0119 0.0103 0.1314 0.0053 0.0119 0.0202 0.0619 0.0132 0.0514 0.0018 0.0057 0.0144 0.0032 0.0022
Vacuole Periphery 0.0366 0.0071 0.1476 0.2069 0.0087 0.009 0.0219 0.0006 0.0043 0.0085 0.0349 0.0096 0.0173 0.0003 0.002 0.0081 0.0019 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 391.8633 344.1562 368.247 392.6644 372.1374 293.7347 333.6064 362.2511 327.559 350.6957
Translational Efficiency 1.9721 2.2432 2.0042 1.7191 1.7979 2.6919 2.192 1.7482 1.9414 1.9288

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description mRNA cap binding protein and translation initiation factor eIF4E; the eIF4E-cap complex is responsible for mediating cap-dependent mRNA translation via interactions with translation initiation factor eIF4G (Tif4631p or Tif4632p); protein abundance increases in response to DNA replication stress; mutants are defective for adhesion and pseudohyphal growth; human homolog EIF4E can complement yeast cdc33 null mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Cdc33

Cdc33


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc33-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available