ORF | |
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Human Ortholog | |
Description | Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | – | 12 | 8 | 4 | 3 | 13 | 15 |
Bud | 6 | 6 | 8 | 20 | 17 | 17 | – | 24 | 15 | 37 | 28 | 32 | 9 | 6 | 2 | 0 | 1 | – | 1 | 3 | 2 | 1 | 7 | 8 |
Bud Neck | 5 | 2 | 4 | 0 | 2 | 0 | – | 4 | 1 | 6 | 3 | 4 | 0 | 0 | 2 | 0 | 0 | – | 8 | 4 | 0 | 1 | 11 | 10 |
Bud Site | 1 | 0 | 1 | 2 | 2 | 2 | – | 6 | 8 | 11 | 5 | 10 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 2 | 1 | 3 | 0 | – | 2 | 0 | 1 | 1 | 3 | 1 | 4 | 3 | 0 | 0 | – | 1 | 1 | 0 | 0 | 1 | 2 |
Cytoplasm | 269 | 198 | 97 | 104 | 107 | 86 | – | 169 | 91 | 152 | 116 | 142 | 184 | 244 | 343 | 11 | 12 | – | 124 | 82 | 27 | 14 | 36 | 47 |
Endoplasmic Reticulum | 7 | 1 | 1 | 1 | 2 | 5 | – | 1 | 0 | 0 | 0 | 3 | 3 | 2 | 3 | 1 | 1 | – | 4 | 2 | 4 | 3 | 5 | 5 |
Endosome | 10 | 17 | 0 | 1 | 32 | 0 | – | 4 | 0 | 2 | 1 | 1 | 9 | 45 | 13 | 4 | 1 | – | 29 | 14 | 8 | 9 | 31 | 29 |
Golgi | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 0 | 1 | 2 | 3 | 6 |
Mitochondria | 151 | 73 | 45 | 239 | 207 | 348 | – | 518 | 374 | 485 | 428 | 575 | 50 | 66 | 27 | 3 | 0 | – | 296 | 210 | 84 | 87 | 243 | 237 |
Nucleus | 1 | 0 | 0 | 2 | 3 | 4 | – | 8 | 8 | 4 | 2 | 7 | 1 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 1 | 2 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 2 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 1 | – | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 2 | 0 |
Peroxisomes | 1 | 0 | 0 | 3 | 17 | 0 | – | 0 | 0 | 3 | 0 | 5 | 0 | 18 | 1 | 0 | 0 | – | 2 | 1 | 3 | 1 | 6 | 3 |
SpindlePole | 8 | 4 | 5 | 7 | 32 | 7 | – | 52 | 24 | 49 | 35 | 34 | 5 | 5 | 6 | 0 | 2 | – | 13 | 9 | 3 | 4 | 16 | 17 |
Vac/Vac Membrane | 33 | 29 | 1 | 5 | 13 | 13 | – | 9 | 6 | 14 | 5 | 6 | 11 | 16 | 20 | 3 | 0 | – | 53 | 36 | 11 | 11 | 37 | 45 |
Unique Cell Count | 438 | 296 | 137 | 310 | 362 | 405 | 679 | 433 | 620 | 500 | 665 | 253 | 365 | 403 | 18 | 17 | 571 | 390 | 159 | 150 | 426 | 444 | ||
Labelled Cell Count | 500 | 336 | 165 | 386 | 437 | 483 | 801 | 528 | 766 | 625 | 824 | 275 | 408 | 421 | 23 | 17 | 571 | 390 | 159 | 150 | 426 | 444 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.4 | 3.0 | 3.1 | 3.7 | 2.9 | – | 2.9 | 2.5 | 2.8 | 2.6 | 2.6 | 4.4 | 4.6 | 4.5 | 5.9 | 5.6 | – | 4.3 | 4.7 | 5.1 |
Std Deviation (1e-4) | 0.9 | 1.4 | 1.1 | 1.0 | 1.3 | 0.7 | – | 0.8 | 0.7 | 0.9 | 1.1 | 0.6 | 0.7 | 0.9 | 0.9 | 1.5 | 1.5 | – | 1.4 | 1.2 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.3 | -0.06 | – | -0.05 | -0.28 | -0.08 | -0.21 | -0.23 | 0.57 | 0.63 | 0.57 | 0.98 | 0.91 | – | 0.52 | 0.66 | 0.76 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1606 | 0.3203 | 0.1162 | -0.2188 | -0.4282 | 0.1614 | -0.3389 | -0.0878 | -0.2563 | -0.7575 | -0.5155 | -0.4577 | 0.9616 | 1.5212 | 1.149 | 1.2993 | 1.0961 | 1.1108 |
Actin | 0.0081 | 0.0055 | 0.0073 | 0.0146 | 0.0024 | 0.0052 | 0.0201 | 0.0095 | 0.0187 | 0.0311 | 0.0103 | 0.0104 | 0.0354 | 0.0098 | 0.0106 | 0.0008 | 0.0015 | 0.0096 |
Bud | 0.0007 | 0.0079 | 0.0015 | 0.0061 | 0.0046 | 0.0011 | 0.0018 | 0.0057 | 0.0038 | 0.0031 | 0.0025 | 0.0017 | 0.0009 | 0.0041 | 0.0034 | 0.0013 | 0.0027 | 0.0026 |
Bud Neck | 0.0009 | 0.0014 | 0.0039 | 0.0011 | 0.0014 | 0.0111 | 0.0047 | 0.0013 | 0.0065 | 0.0059 | 0.0009 | 0.0119 | 0.0039 | 0.002 | 0.0055 | 0.001 | 0.0084 | 0.01 |
Bud Periphery | 0.0015 | 0.0103 | 0.004 | 0.017 | 0.0273 | 0.0026 | 0.0055 | 0.0092 | 0.0094 | 0.0171 | 0.0063 | 0.007 | 0.0023 | 0.0072 | 0.0101 | 0.0024 | 0.0054 | 0.0068 |
Bud Site | 0.0022 | 0.0222 | 0.0076 | 0.0103 | 0.0019 | 0.0027 | 0.009 | 0.0377 | 0.0186 | 0.0022 | 0.0019 | 0.0018 | 0.0068 | 0.0431 | 0.0104 | 0.0014 | 0.0113 | 0.0028 |
Cell Periphery | 0.0009 | 0.0022 | 0.0028 | 0.0015 | 0.0013 | 0.0008 | 0.0055 | 0.0014 | 0.0024 | 0.0024 | 0.0005 | 0.0016 | 0.0032 | 0.0031 | 0.0055 | 0.0005 | 0.0035 | 0.0015 |
Cytoplasm | 0.0677 | 0.0959 | 0.0582 | 0.0808 | 0.0599 | 0.0815 | 0.0829 | 0.1076 | 0.0788 | 0.0849 | 0.0742 | 0.0854 | 0.0857 | 0.1603 | 0.1515 | 0.0768 | 0.0773 | 0.1604 |
Cytoplasmic Foci | 0.129 | 0.1409 | 0.1276 | 0.0944 | 0.1699 | 0.1485 | 0.1519 | 0.175 | 0.133 | 0.0956 | 0.1491 | 0.1328 | 0.1583 | 0.1242 | 0.1066 | 0.0653 | 0.192 | 0.1412 |
Eisosomes | 0.0008 | 0.0016 | 0.007 | 0.0007 | 0.0001 | 0.0003 | 0.001 | 0.0008 | 0.0013 | 0.0005 | 0.0002 | 0.001 | 0.0017 | 0.0008 | 0.0009 | 0.0002 | 0.0002 | 0.0006 |
Endoplasmic Reticulum | 0.0119 | 0.0066 | 0.0052 | 0.0045 | 0.0023 | 0.0035 | 0.0097 | 0.0037 | 0.0029 | 0.0065 | 0.0056 | 0.0041 | 0.0177 | 0.0149 | 0.0104 | 0.0038 | 0.0054 | 0.0138 |
Endosome | 0.1724 | 0.1861 | 0.1349 | 0.1305 | 0.1594 | 0.1498 | 0.1995 | 0.1533 | 0.1425 | 0.178 | 0.2243 | 0.1477 | 0.1761 | 0.1486 | 0.1693 | 0.1577 | 0.2984 | 0.1459 |
Golgi | 0.0374 | 0.0609 | 0.0447 | 0.0399 | 0.0516 | 0.0463 | 0.0658 | 0.0546 | 0.0468 | 0.0453 | 0.0572 | 0.0556 | 0.0401 | 0.0493 | 0.0474 | 0.0571 | 0.0577 | 0.0565 |
Lipid Particles | 0.0444 | 0.0165 | 0.0248 | 0.008 | 0.0124 | 0.028 | 0.0368 | 0.0187 | 0.0184 | 0.0044 | 0.0249 | 0.033 | 0.0535 | 0.0085 | 0.0203 | 0.0031 | 0.0169 | 0.0169 |
Mitochondria | 0.2138 | 0.2111 | 0.3153 | 0.4087 | 0.2796 | 0.3217 | 0.2056 | 0.1685 | 0.3256 | 0.3189 | 0.2504 | 0.3201 | 0.1089 | 0.1982 | 0.2582 | 0.4021 | 0.2538 | 0.2386 |
None | 0.2412 | 0.169 | 0.1893 | 0.1385 | 0.1144 | 0.1367 | 0.1233 | 0.1841 | 0.1175 | 0.1639 | 0.1255 | 0.1131 | 0.232 | 0.1836 | 0.1388 | 0.187 | 0.007 | 0.1411 |
Nuclear Periphery | 0.0065 | 0.0029 | 0.0017 | 0.0026 | 0.0007 | 0.0021 | 0.0056 | 0.0016 | 0.0018 | 0.0011 | 0.0023 | 0.0029 | 0.0073 | 0.0028 | 0.0046 | 0.001 | 0.0014 | 0.0013 |
Nucleolus | 0.0013 | 0.0013 | 0.0015 | 0.0016 | 0.0006 | 0.0008 | 0.0014 | 0.0018 | 0.0019 | 0.0005 | 0.0009 | 0.001 | 0.0011 | 0.0007 | 0.0007 | 0.0004 | 0.0005 | 0.0004 |
Nucleus | 0.0026 | 0.0024 | 0.0023 | 0.0027 | 0.0011 | 0.0031 | 0.0039 | 0.0026 | 0.0047 | 0.0013 | 0.0018 | 0.0017 | 0.0029 | 0.0018 | 0.0016 | 0.0009 | 0.0012 | 0.0012 |
Peroxisomes | 0.0325 | 0.0301 | 0.0383 | 0.0146 | 0.0927 | 0.0358 | 0.044 | 0.0413 | 0.0449 | 0.0247 | 0.0368 | 0.0464 | 0.0361 | 0.015 | 0.0242 | 0.0073 | 0.0333 | 0.0328 |
Punctate Nuclear | 0.0031 | 0.001 | 0.0016 | 0.0011 | 0.0006 | 0.0016 | 0.0033 | 0.0057 | 0.0027 | 0.0017 | 0.002 | 0.003 | 0.0042 | 0.0007 | 0.0008 | 0.0005 | 0.0006 | 0.0009 |
Vacuole | 0.0156 | 0.0185 | 0.0152 | 0.0141 | 0.012 | 0.0111 | 0.0133 | 0.0125 | 0.0127 | 0.0075 | 0.0143 | 0.0118 | 0.017 | 0.0159 | 0.0122 | 0.0104 | 0.0157 | 0.0105 |
Vacuole Periphery | 0.0055 | 0.0057 | 0.0053 | 0.0067 | 0.0037 | 0.0055 | 0.0053 | 0.0033 | 0.0052 | 0.0036 | 0.008 | 0.006 | 0.0047 | 0.0052 | 0.0069 | 0.0192 | 0.0058 | 0.0046 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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