Standard name | |
---|---|
Human Ortholog | |
Description | Phospholipase; contains lipase specific GXSXG motif; maintains lipid droplet (LD) morphology; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 8 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 5 | 3 | 9 | 4 | 7 | 10 | 8 | 8 | 18 | 3 | 7 | 10 |
Bud | 1 | 0 | 1 | 4 | 8 | 15 | 30 | 23 | 13 | 26 | 9 | 15 | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 1 | 1 | 1 | 7 | 10 |
Bud Neck | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 7 | 0 | 0 | 0 | 5 | 5 | 4 | 2 | 5 | 11 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 7 | 0 | 12 | 9 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 3 | 2 | 4 | 1 | 4 | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 6 | 6 | 6 | 8 |
Cytoplasm | 167 | 147 | 238 | 143 | 250 | 194 | 230 | 241 | 137 | 233 | 92 | 152 | 245 | 266 | 231 | 27 | 32 | 25 | 88 | 88 | 71 | 205 | 255 | 235 |
Endoplasmic Reticulum | 16 | 22 | 21 | 9 | 4 | 12 | 6 | 2 | 24 | 0 | 0 | 3 | 25 | 23 | 44 | 16 | 14 | 5 | 60 | 36 | 106 | 24 | 34 | 60 |
Endosome | 3 | 1 | 9 | 4 | 9 | 2 | 4 | 11 | 2 | 2 | 0 | 1 | 6 | 19 | 65 | 124 | 122 | 89 | 8 | 10 | 18 | 1 | 13 | 16 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 14 | 5 | 0 | 1 | 5 | 0 | 1 | 3 |
Mitochondria | 20 | 9 | 19 | 53 | 66 | 197 | 361 | 364 | 311 | 488 | 320 | 332 | 3 | 0 | 1 | 26 | 29 | 19 | 53 | 25 | 112 | 28 | 53 | 47 |
Nucleus | 0 | 1 | 1 | 0 | 2 | 1 | 5 | 9 | 2 | 3 | 3 | 8 | 0 | 4 | 2 | 4 | 4 | 2 | 1 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 5 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 3 | 3 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 4 | 7 | 4 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 7 | 2 | 0 | 1 | 3 | 14 | 7 | 5 | 3 | 9 | 4 | 13 | 29 | 35 | 38 | 25 | 1 | 0 | 5 | 2 | 3 | 7 |
Vac/Vac Membrane | 3 | 4 | 7 | 3 | 12 | 12 | 22 | 26 | 15 | 28 | 18 | 28 | 3 | 15 | 11 | 35 | 30 | 27 | 19 | 15 | 14 | 11 | 22 | 31 |
Unique Cell Count | 201 | 174 | 298 | 195 | 311 | 360 | 541 | 566 | 434 | 640 | 368 | 440 | 284 | 329 | 373 | 216 | 232 | 169 | 257 | 199 | 374 | 296 | 421 | 450 |
Labelled Cell Count | 215 | 185 | 316 | 222 | 357 | 439 | 668 | 702 | 520 | 800 | 458 | 557 | 293 | 347 | 406 | 290 | 302 | 221 | 257 | 199 | 374 | 296 | 421 | 450 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.8 | 4.4 | 4.1 | 4.7 | 3.8 | 3.7 | 3.6 | 3.3 | 3.3 | 2.8 | 2.9 | 5.8 | 6.1 | 6.0 | 7.9 | 8.0 | 7.6 | 5.7 | 6.0 | 6.5 |
Std Deviation (1e-4) | 0.6 | 0.9 | 0.8 | 0.9 | 1.8 | 1.2 | 1.4 | 1.5 | 1.0 | 1.2 | 0.7 | 1.0 | 1.0 | 1.1 | 1.1 | 1.5 | 1.8 | 1.4 | 1.1 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.11 | -0.19 | -0.25 | -0.27 | -0.41 | -0.43 | -0.67 | -0.59 | 0.4 | 0.47 | 0.45 | 0.84 | 0.86 | 0.8 | 0.37 | 0.45 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.7 | 4.7 | 5.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2132 | 4.374 | 4.0365 | 4.1922 | 3.7017 | 4.1388 | 1.8039 | 2.0219 | 1.8627 | 1.5081 | 1.3457 | 2.008 | 0.9009 | 1.0422 | 0.9192 | 1.0739 | 0.7854 | 1.016 |
Actin | 0.0066 | 0.0022 | 0.0048 | 0.0093 | 0.0021 | 0.0021 | 0.0244 | 0.0014 | 0.0074 | 0.022 | 0.0078 | 0.0036 | 0.0064 | 0.0006 | 0.0053 | 0.0142 | 0.011 | 0.0014 |
Bud | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0007 | 0.0006 | 0.0023 | 0.0008 | 0.0013 | 0.0001 | 0.0002 | 0.0006 |
Bud Neck | 0.0011 | 0.0011 | 0.0012 | 0.0013 | 0.0013 | 0.0034 | 0.0046 | 0.0022 | 0.0071 | 0.0013 | 0.0018 | 0.0027 | 0.0054 | 0.0005 | 0.0007 | 0.0005 | 0.001 | 0.001 |
Bud Periphery | 0.0002 | 0.0002 | 0.0003 | 0.0013 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0008 | 0.0013 | 0.0009 | 0.0027 | 0.0014 | 0.0005 | 0.0002 | 0.0003 | 0.0004 |
Bud Site | 0.0047 | 0.0068 | 0.0018 | 0.0007 | 0.0005 | 0.0006 | 0.0024 | 0.0197 | 0.0045 | 0.0023 | 0.0011 | 0.0005 | 0.0049 | 0.0088 | 0.0068 | 0.0007 | 0.0005 | 0.0003 |
Cell Periphery | 0.001 | 0.0018 | 0.0015 | 0.002 | 0.0031 | 0.0008 | 0.0009 | 0.0006 | 0.0008 | 0.0005 | 0.0003 | 0.0005 | 0.0023 | 0.0005 | 0.0005 | 0.0005 | 0.0004 | 0.0003 |
Cytoplasm | 0.1177 | 0.1845 | 0.1396 | 0.2533 | 0.3455 | 0.182 | 0.0992 | 0.1679 | 0.1647 | 0.1156 | 0.1105 | 0.1893 | 0.1109 | 0.1005 | 0.1058 | 0.1942 | 0.0963 | 0.1493 |
Cytoplasmic Foci | 0.1398 | 0.1194 | 0.1186 | 0.1379 | 0.1078 | 0.1246 | 0.1058 | 0.1102 | 0.1229 | 0.138 | 0.1183 | 0.1172 | 0.122 | 0.1505 | 0.1536 | 0.1285 | 0.2245 | 0.133 |
Eisosomes | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.1129 | 0.0939 | 0.0707 | 0.0721 | 0.1179 | 0.121 | 0.0827 | 0.0978 | 0.1017 | 0.069 | 0.1441 | 0.115 | 0.0665 | 0.0746 | 0.1101 | 0.0703 | 0.0845 | 0.0956 |
Endosome | 0.1798 | 0.182 | 0.2045 | 0.1586 | 0.1168 | 0.1593 | 0.265 | 0.2885 | 0.2496 | 0.2478 | 0.2788 | 0.2135 | 0.239 | 0.3157 | 0.2685 | 0.3388 | 0.2329 | 0.2322 |
Golgi | 0.0217 | 0.0396 | 0.0402 | 0.0321 | 0.0188 | 0.0215 | 0.0264 | 0.0195 | 0.0325 | 0.0464 | 0.0238 | 0.0242 | 0.0266 | 0.0332 | 0.0326 | 0.0313 | 0.0351 | 0.0297 |
Lipid Particles | 0.3213 | 0.2387 | 0.261 | 0.1745 | 0.1233 | 0.241 | 0.2651 | 0.1899 | 0.1812 | 0.2592 | 0.2457 | 0.2473 | 0.2847 | 0.2311 | 0.1679 | 0.1421 | 0.2263 | 0.2422 |
Mitochondria | 0.0019 | 0.008 | 0.0157 | 0.0035 | 0.0011 | 0.0055 | 0.0095 | 0.0024 | 0.0047 | 0.0099 | 0.002 | 0.0047 | 0.013 | 0.0026 | 0.0152 | 0.0039 | 0.0044 | 0.0066 |
None | 0.051 | 0.0828 | 0.0916 | 0.0767 | 0.127 | 0.1029 | 0.035 | 0.044 | 0.0586 | 0.0175 | 0.0089 | 0.0202 | 0.0511 | 0.0248 | 0.0675 | 0.0187 | 0.0406 | 0.0524 |
Nuclear Periphery | 0.0092 | 0.007 | 0.0046 | 0.0052 | 0.0041 | 0.0072 | 0.0251 | 0.0125 | 0.0125 | 0.0132 | 0.0237 | 0.0161 | 0.0077 | 0.0107 | 0.0116 | 0.0153 | 0.0062 | 0.0122 |
Nucleolus | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0.0011 | 0.0032 | 0.0008 | 0.0004 | 0.0004 | 0.0008 | 0.0027 | 0.0012 | 0.0008 | 0.0004 | 0.0002 | 0.0004 |
Nucleus | 0.0011 | 0.0014 | 0.0014 | 0.0018 | 0.0019 | 0.0018 | 0.0061 | 0.0027 | 0.0068 | 0.0022 | 0.0042 | 0.0045 | 0.0096 | 0.002 | 0.0039 | 0.0045 | 0.0014 | 0.0037 |
Peroxisomes | 0.0121 | 0.0124 | 0.0179 | 0.0422 | 0.0176 | 0.009 | 0.0102 | 0.0042 | 0.0191 | 0.0261 | 0.0042 | 0.0087 | 0.0109 | 0.0157 | 0.0238 | 0.0099 | 0.0221 | 0.0115 |
Punctate Nuclear | 0.0038 | 0.0049 | 0.0049 | 0.007 | 0.0019 | 0.0037 | 0.0123 | 0.0074 | 0.0075 | 0.0136 | 0.0061 | 0.0076 | 0.0136 | 0.0057 | 0.0088 | 0.0122 | 0.0042 | 0.0112 |
Vacuole | 0.0121 | 0.0108 | 0.017 | 0.0171 | 0.0076 | 0.0112 | 0.0205 | 0.023 | 0.0149 | 0.0111 | 0.014 | 0.0195 | 0.0157 | 0.0174 | 0.0122 | 0.0119 | 0.0065 | 0.0141 |
Vacuole Periphery | 0.0012 | 0.0016 | 0.0017 | 0.0025 | 0.0009 | 0.0015 | 0.0028 | 0.0023 | 0.002 | 0.0024 | 0.0021 | 0.0026 | 0.0019 | 0.0016 | 0.0024 | 0.0018 | 0.0012 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.3105 | 16.7677 | 12.9833 | 17.108 | 20.0899 | 3.2603 | 21.0079 | 17.4127 | 20.5159 | 18.6003 |
Translational Efficiency | 0.5571 | 0.9319 | 0.6644 | 0.6008 | 0.3686 | 3.7287 | 1.0598 | 0.8828 | 0.6449 | 0.4629 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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