Standard name
Human Ortholog
Description Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.12 0.15 0.15 0.1 0.09 0.14 0.11 0.14 0.1 0.07 0.12 0.14 0.07 0.06 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.07 0.05 0 0.1 0.12 0.06 0.1 0.17 0.11 0.15 0 0 0 0.07 0 0.1 0 0 0 0 0 0
Nucleus 0.8 0.85 0.73 0.74 0.71 0.63 0.59 0.69 0.58 0.58 0.62 0.52 0.8 0.68 0.66 0.77 0.77 0.65 0.57 0.79 0.66 0.53 0.5 0.46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.53 0.47 0.64 0.45 0.29 0.44 0.43 0.45 0.48 0.46 0.46 0.57 0.5 0.45 0.38 0.31 0.44 0.36 0.36 0.15 0.29 0.37 0.4 0.43
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 0 2 0 0 0 1 0 0 0 0 0 2 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 1 0 0 0 0 4 4 7 6 1 8 0 2 1 1 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 3 1 12 19 17 23 23 23 22 16 16 10 23 27 10 5 8 1 1 0 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 3 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0
Mitochondria 4 11 8 5 4 11 28 15 16 35 13 25 0 1 0 10 1 8 0 1 1 0 0 0
Nucleus 94 199 81 75 88 73 132 182 97 116 71 86 111 133 132 111 67 50 70 193 74 46 74 79
Nuclear Periphery 0 0 0 2 0 0 4 0 3 0 1 1 0 0 1 4 0 0 1 0 0 0 0 0
Nucleolus 62 110 71 46 36 51 97 119 81 92 52 94 70 88 75 44 38 28 44 37 32 32 59 73
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 3 6 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 1 0 4 8 0 0 0 0 0 0 1 0 3
Vac/Vac Membrane 0 0 0 0 0 0 2 0 0 0 1 0 0 0 2 2 1 1 1 3 0 0 4 8
Unique Cell Count 117 235 111 102 124 115 224 265 168 200 114 165 139 196 199 144 87 77 123 246 112 88 148 171
Labelled Cell Count 162 324 161 140 148 154 290 343 227 274 156 231 191 256 254 183 112 96 123 246 112 88 148 171


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.8 11.1 9.7 8.6 9.8 8.7 7.1 7.8 7.1 6.7 6.8 7.1 12.7 12.2 11.8 13.7 13.9 16.2 10.2 11.9 12.6
Std Deviation (1e-4) 2.5 3.0 2.7 2.5 3.5 3.9 2.3 2.3 2.0 2.1 2.1 2.4 4.0 4.5 4.5 6.0 4.9 4.6 2.6 3.4 3.1
Intensity Change (Log2) – – – -0.18 0.01 -0.16 -0.44 -0.31 -0.45 -0.54 -0.51 -0.44 0.39 0.34 0.28 0.5 0.52 0.74 0.07 0.3 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.3 4.0 3.9 3.1 2.8 3.7 3.3 3.7 3.0 0 3.4 3.7 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.7 -1.4 0.6 1.5 -0.6 0.7 2.5 1.1 2.0 0 0 0 -0.1 0 0.8
Nucleus 0.1 -0.3 -1.5 -2.5 -0.8 -2.6 -2.6 -1.7 -3.5 1.3 -0.9 -1.2 0.8 0.6 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.8 -5.4 -3.0 -3.6 -3.4 -2.6 -3.0 -2.8 -1.2 -2.2 -3.2 -4.4 -5.3 -2.8 -3.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.7507 9.6271 9.3181 7.4871 9.2691 9.2274 8.8623 10.3695 8.8962 7.8178 6.9526 8.451 11.9303 10.9098 11.1761 11.1566 10.5759 10.8673
Actin 0.0077 0 0 0 0 0 0.0224 0.0001 0.0284 0.0005 0.0037 0.0064 0 0 0.0119 0 0 0
Bud 0.0001 0 0 0 0.0001 0 0.0007 0 0.0003 0.0003 0.0005 0.0001 0.0002 0 0.0001 0 0.0001 0
Bud Neck 0.0001 0 0 0 0.0003 0.0003 0.0014 0.0001 0.0002 0.0001 0.0005 0.0003 0.0002 0 0.0001 0 0.0003 0.0002
Bud Periphery 0.0001 0 0 0 0.0002 0.0001 0.0007 0.0001 0.0003 0.0009 0.0011 0.0002 0.0004 0 0.0002 0 0.0002 0.0001
Bud Site 0.0001 0 0 0 0.0002 0.0001 0.0299 0.0001 0.0037 0.0011 0.0018 0.0002 0.0003 0 0.0002 0.0001 0.0002 0
Cell Periphery 0.0001 0 0 0.0001 0.0003 0.0001 0.0002 0 0.0001 0.0005 0.0007 0 0.0002 0 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0001 0 0 0 0 0 0.0019 0 0.0054 0.0006 0.0054 0.0006 0.0002 0 0 0.0004 0 0
Cytoplasmic Foci 0.0008 0 0 0 0 0 0.0185 0 0.0034 0.0449 0.0195 0.003 0.0007 0 0.0001 0.0001 0 0
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0001 0.0002 0.0002 0 0 0 0.0003 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0005 0 0.0017 0.0002 0.0025 0.0005 0.0001 0 0 0 0 0
Endosome 0.0002 0 0 0 0 0 0.0059 0.0001 0.0068 0.0044 0.0151 0.0061 0.0072 0 0.0001 0 0.0001 0
Golgi 0.0007 0 0 0 0 0 0.0025 0 0.0014 0.0014 0.0051 0.0047 0.0008 0 0.0004 0 0 0
Lipid Particles 0.0008 0 0 0.0002 0 0 0.0042 0 0.0015 0.008 0.0356 0.002 0.0031 0 0.0002 0 0.0001 0
Mitochondria 0.0011 0.0001 0.0001 0.0017 0.0017 0.0001 0.0013 0.002 0.0164 0.0295 0.0629 0.0021 0.0015 0.0001 0.0014 0.0003 0.0009 0.0012
None 0.0001 0 0 0 0 0 0.0011 0 0.0025 0.0012 0.0082 0.0002 0.0002 0 0.0001 0.0003 0.0001 0
Nuclear Periphery 0.0002 0.0002 0.0001 0.0001 0.0001 0 0.0021 0.0043 0.004 0.0035 0.0095 0.0088 0.0005 0.0001 0.0001 0.0005 0.0003 0
Nucleolus 0.1423 0.0891 0.0673 0.1293 0.4917 0.3112 0.1136 0.0526 0.0545 0.0683 0.2797 0.2343 0.1107 0.0676 0.0637 0.0583 0.3747 0.2184
Nucleus 0.8415 0.9099 0.9325 0.867 0.5043 0.6878 0.781 0.9394 0.8626 0.8131 0.4924 0.7255 0.8662 0.9318 0.92 0.938 0.6209 0.7789
Peroxisomes 0.0033 0 0 0 0 0 0.0025 0 0.0018 0.0112 0.0302 0.0015 0.0002 0 0.0003 0 0 0
Punctate Nuclear 0.0001 0.0006 0 0.0001 0.0004 0.0002 0.0085 0.0003 0.0028 0.0054 0.0176 0.003 0.0008 0.0002 0 0.0016 0.0017 0.0001
Vacuole 0.0004 0 0 0.0003 0.0003 0.0001 0.0006 0.0001 0.0017 0.0014 0.0041 0.0002 0.0048 0 0.0005 0.0002 0.0002 0.0003
Vacuole Periphery 0.0004 0 0 0.0011 0.0002 0 0.0002 0.0006 0.0003 0.0034 0.0038 0.0002 0.0015 0 0.0003 0.0002 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.1923 21.2124 30.4317 27.7141 32.3041 29.2497 28.7758 26.539 32.9957 26.4086
Translational Efficiency 3.6599 3.0701 2.078 2.5635 1.933 2.1296 2.0354 1.9813 1.5573 2.2406

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Leo1

Leo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Leo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available