Standard name
Human Ortholog
Description GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; RGA1 has a paralog, RGA2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.31 0.17 0.18 0.1 0 0 0 0 0 0 0 0.09 0.06 0 0.16 0.25 0.26 0.19 0.09 0.14 0.08 0.13 0.09
Bud 0 0.05 0.07 0 0.05 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0.21 0.12 0.11 0.12 0.05 0
Bud Neck 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.34 0.36 0.27 0.23 0.17 0.08
Bud Site 0.12 0.06 0.11 0.11 0 0 0.06 0 0 0 0 0.08 0 0 0.05 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.42 0.65 0.61 0.62 0.72 0.75 0.72 0.74 0.7 0.51 0.52 0.75 0.79 0.91 0.62 0.62 0.62 0.18 0.27 0.29 0.41 0.5 0.61
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.06 0.17 0.09 0.17 0.26 0.29 0.44 0.59 0.66 0 0 0 0 0 0 0 0 0.08 0 0 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.2 0.05 0 0.09 0.07 0.07 0 0 0 0 0 0.11 0.06 0 0.06 0 0 0 0 0 0 0 0
SpindlePole 0.15 0 0.06 0.06 0.08 0 0.07 0 0 0 0 0.05 0.06 0 0.1 0.07 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 72 34 42 17 2 11 8 5 0 3 1 17 13 8 30 24 29 56 22 41 14 26 17
Bud 8 10 16 7 11 7 10 13 11 5 7 3 6 9 4 2 4 62 32 32 21 9 4
Bud Neck 1 3 12 2 2 2 1 10 2 0 0 1 1 0 2 1 1 101 96 81 42 36 14
Bud Site 29 11 25 19 8 11 17 7 7 3 4 15 9 5 9 1 4
Cell Periphery 4 1 2 3 6 5 4 4 4 3 0 4 6 11 12 3 3 0 1 3 1 2 1
Cytoplasm 98 126 142 104 166 205 221 274 155 77 73 139 180 253 119 59 69 52 71 88 74 103 115
Endoplasmic Reticulum 5 10 7 3 2 6 4 3 1 0 1 4 4 3 8 6 2 0 2 2 3 3 2
Endosome 8 3 2 2 13 2 8 2 2 0 0 3 7 3 8 4 2 6 9 9 2 4 6
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 1 0 4 0 1 2 0 0 2 1 1
Mitochondria 1 9 14 29 21 46 79 108 98 89 93 2 2 5 8 3 4 4 10 25 6 6 11
Nucleus 4 0 1 0 0 2 1 0 1 0 4 0 0 2 1 5 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 1 0
Peroxisomes 46 9 6 16 17 19 9 7 4 2 3 21 14 6 11 1 4 1 0 0 0 0 1
SpindlePole 35 5 15 10 19 10 22 10 8 2 2 9 13 6 20 7 7 3 5 4 2 0 0
Vac/Vac Membrane 4 0 1 0 3 0 3 0 1 0 0 0 0 1 12 2 2 2 11 10 6 5 8
Unique Cell Count 233 195 231 169 231 273 309 369 222 150 141 185 228 277 193 95 111 299 268 305 182 206 189
Labelled Cell Count 316 221 286 214 271 327 387 443 294 185 189 218 256 312 248 119 135 299 268 305 182 206 189


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 4.7 4.3 4.2 4.6 3.9 3.7 3.6 3.3 3.2 3.3 5.3 5.5 5.0 7.3 7.2 7.5 5.7 5.9 6.6
Std Deviation (1e-4) 0.8 0.7 0.7 1.2 1.5 1.3 1.1 1.3 1.6 1.1 1.4 1.0 1.2 1.3 1.5 1.6 1.5 1.6 1.5 1.7
Intensity Change (Log2) -0.05 0.1 -0.14 -0.22 -0.24 -0.39 -0.41 -0.36 0.31 0.35 0.21 0.77 0.75 0.81 0.42 0.46 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6531 1.923 1.6572 1.5719 1.0686 1.6545 -0.5791 0.1669 0.2465 0.0691 -0.3519 0.1211 3.6549 4.5324 3.726 2.7958 3.5736 3.425
Actin 0.0662 0.0133 0.0266 0.0069 0.0045 0.0285 0.0261 0.014 0.0282 0.0127 0.0028 0.0243 0.0245 0.0828 0.0096 0.0297 0.0384 0.041
Bud 0.0032 0.007 0.0065 0.0007 0.0011 0.0005 0.0044 0.0074 0.0071 0.0024 0.0004 0.0035 0.0013 0.0022 0.002 0.0024 0.003 0.002
Bud Neck 0.1777 0.0232 0.2216 0.6749 0.8487 0.7679 0.1284 0.0215 0.1177 0.6385 0.8324 0.5783 0.0958 0.0079 0.1457 0.6218 0.6675 0.6426
Bud Periphery 0.0073 0.0117 0.0142 0.0007 0.0022 0.0011 0.0052 0.0084 0.0084 0.0034 0.0009 0.0075 0.0016 0.0037 0.002 0.004 0.0021 0.0076
Bud Site 0.1982 0.2486 0.1114 0.0072 0.0152 0.0029 0.1968 0.2312 0.0552 0.0241 0.0224 0.0066 0.2919 0.1861 0.1413 0.0604 0.0069 0.0053
Cell Periphery 0.0117 0.0097 0.007 0.0012 0.001 0.002 0.0043 0.0033 0.0017 0.001 0.0006 0.0013 0.0046 0.0072 0.0056 0.0054 0.0017 0.0029
Cytoplasm 0.1947 0.4534 0.3938 0.1187 0.1089 0.1288 0.3004 0.4508 0.4677 0.1496 0.0452 0.2133 0.0641 0.3327 0.326 0.1348 0.0577 0.1786
Cytoplasmic Foci 0.0404 0.0183 0.0219 0.0316 0.0099 0.009 0.0552 0.0189 0.0357 0.0461 0.0136 0.0399 0.116 0.0116 0.0181 0.0327 0.0434 0.045
Eisosomes 0.0016 0.0009 0.0006 0.0001 0 0.0003 0.0005 0.0003 0.0003 0.0001 0.0001 0.0004 0.0022 0.0019 0.0011 0.0008 0.0007 0.0003
Endoplasmic Reticulum 0.008 0.0034 0.0063 0.0009 0.0004 0.0007 0.0052 0.0028 0.0051 0.0038 0.0038 0.0024 0.0021 0.0027 0.007 0.0009 0.001 0.0012
Endosome 0.025 0.0027 0.0179 0.0268 0.0012 0.0076 0.0189 0.0079 0.0157 0.016 0.0096 0.0092 0.0103 0.0012 0.0112 0.0008 0.0011 0.0013
Golgi 0.0096 0.0011 0.0093 0.006 0.0006 0.0045 0.0041 0.002 0.0032 0.0048 0.0006 0.0032 0.003 0.0014 0.0122 0.0013 0.0025 0.001
Lipid Particles 0.0207 0.0007 0.0045 0.0031 0.0001 0.0006 0.0025 0.0006 0.0014 0.0038 0.0002 0.0016 0.0173 0.0008 0.0028 0.0005 0.0219 0.0002
Mitochondria 0.0237 0.0007 0.0071 0.0038 0.0001 0.0039 0.0017 0.0007 0.0078 0.0066 0.0007 0.0015 0.0015 0.0072 0.0053 0.0002 0.0009 0.0002
None 0.1492 0.1913 0.1303 0.0891 0.0034 0.0244 0.2129 0.2163 0.2185 0.0534 0.0289 0.0923 0.3166 0.3432 0.2962 0.0856 0.0724 0.062
Nuclear Periphery 0.0163 0.0006 0.0052 0.0001 0.0001 0.0034 0.0064 0.0009 0.0026 0.0113 0.0276 0.0035 0.0034 0.0007 0.0007 0.0002 0.0002 0.0002
Nucleolus 0.0021 0.0006 0.0008 0.0003 0.0002 0.0005 0.0031 0.0009 0.0011 0.0007 0.0007 0.0006 0.0044 0.0005 0.0004 0.0007 0.0007 0.0001
Nucleus 0.0081 0.0042 0.0025 0.0005 0.0011 0.0099 0.0099 0.0044 0.0059 0.0037 0.0051 0.0027 0.0052 0.0011 0.0012 0.0014 0.0013 0.0005
Peroxisomes 0.0147 0.0005 0.003 0.0202 0.0002 0.0013 0.0023 0.0006 0.0069 0.005 0.0003 0.0029 0.0098 0.0028 0.0074 0.0013 0.0721 0.0068
Punctate Nuclear 0.0113 0.0013 0.0019 0.0004 0.0006 0.0006 0.0049 0.002 0.0032 0.0049 0.0014 0.0022 0.0211 0.0012 0.0009 0.0139 0.0033 0.0007
Vacuole 0.0072 0.0066 0.0061 0.0062 0.0005 0.0009 0.0061 0.0049 0.0058 0.0059 0.0018 0.0021 0.0027 0.001 0.003 0.001 0.001 0.0005
Vacuole Periphery 0.0029 0.0004 0.0016 0.0006 0 0.0006 0.0007 0.0003 0.0007 0.0019 0.001 0.0005 0.0005 0.0001 0.0003 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.2373 13.3735 17.185 36.2563 19.8696 16.7753 17.2322 22.816 28.4672 26.5184
Translational Efficiency 0.6286 0.6712 0.8311 0.478 0.4982 0.9419 0.6262 0.4222 0.563 0.5666

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; RGA1 has a paralog, RGA2, that arose from the whole genome duplication
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Rga1

Rga1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rga1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available