Standard name | |
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Human Ortholog | |
Description | GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; RGA1 has a paralog, RGA2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 72 | 34 | 42 | 17 | 2 | 11 | 8 | 5 | 0 | – | 3 | 1 | 17 | 13 | 8 | 30 | 24 | 29 | 56 | 22 | 41 | 14 | 26 | 17 |
Bud | 8 | 10 | 16 | 7 | 11 | 7 | 10 | 13 | 11 | – | 5 | 7 | 3 | 6 | 9 | 4 | 2 | 4 | 62 | 32 | 32 | 21 | 9 | 4 |
Bud Neck | 1 | 3 | 12 | 2 | 2 | 2 | 1 | 10 | 2 | – | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 101 | 96 | 81 | 42 | 36 | 14 |
Bud Site | 29 | 11 | 25 | 19 | 8 | 11 | 17 | 7 | 7 | – | 3 | 4 | 15 | 9 | 5 | 9 | 1 | 4 | – | – | – | – | – | – |
Cell Periphery | 4 | 1 | 2 | 3 | 6 | 5 | 4 | 4 | 4 | – | 3 | 0 | 4 | 6 | 11 | 12 | 3 | 3 | 0 | 1 | 3 | 1 | 2 | 1 |
Cytoplasm | 98 | 126 | 142 | 104 | 166 | 205 | 221 | 274 | 155 | – | 77 | 73 | 139 | 180 | 253 | 119 | 59 | 69 | 52 | 71 | 88 | 74 | 103 | 115 |
Endoplasmic Reticulum | 5 | 10 | 7 | 3 | 2 | 6 | 4 | 3 | 1 | – | 0 | 1 | 4 | 4 | 3 | 8 | 6 | 2 | 0 | 2 | 2 | 3 | 3 | 2 |
Endosome | 8 | 3 | 2 | 2 | 13 | 2 | 8 | 2 | 2 | – | 0 | 0 | 3 | 7 | 3 | 8 | 4 | 2 | 6 | 9 | 9 | 2 | 4 | 6 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 1 | 2 | 0 | 0 | 2 | 1 | 1 |
Mitochondria | 1 | 9 | 14 | 29 | 21 | 46 | 79 | 108 | 98 | – | 89 | 93 | 2 | 2 | 5 | 8 | 3 | 4 | 4 | 10 | 25 | 6 | 6 | 11 |
Nucleus | 4 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 1 | – | 0 | 4 | 0 | 0 | 2 | 1 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 46 | 9 | 6 | 16 | 17 | 19 | 9 | 7 | 4 | – | 2 | 3 | 21 | 14 | 6 | 11 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 35 | 5 | 15 | 10 | 19 | 10 | 22 | 10 | 8 | – | 2 | 2 | 9 | 13 | 6 | 20 | 7 | 7 | 3 | 5 | 4 | 2 | 0 | 0 |
Vac/Vac Membrane | 4 | 0 | 1 | 0 | 3 | 0 | 3 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 12 | 2 | 2 | 2 | 11 | 10 | 6 | 5 | 8 |
Unique Cell Count | 233 | 195 | 231 | 169 | 231 | 273 | 309 | 369 | 222 | 150 | 141 | 185 | 228 | 277 | 193 | 95 | 111 | 299 | 268 | 305 | 182 | 206 | 189 | |
Labelled Cell Count | 316 | 221 | 286 | 214 | 271 | 327 | 387 | 443 | 294 | 185 | 189 | 218 | 256 | 312 | 248 | 119 | 135 | 299 | 268 | 305 | 182 | 206 | 189 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 4.7 | 4.3 | 4.2 | 4.6 | 3.9 | 3.7 | 3.6 | 3.3 | – | 3.2 | 3.3 | 5.3 | 5.5 | 5.0 | 7.3 | 7.2 | 7.5 | 5.7 | 5.9 | 6.6 |
Std Deviation (1e-4) | 0.8 | 0.7 | 0.7 | 1.2 | 1.5 | 1.3 | 1.1 | 1.3 | 1.6 | – | 1.1 | 1.4 | 1.0 | 1.2 | 1.3 | 1.5 | 1.6 | 1.5 | 1.6 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.1 | -0.14 | -0.22 | -0.24 | -0.39 | – | -0.41 | -0.36 | 0.31 | 0.35 | 0.21 | 0.77 | 0.75 | 0.81 | 0.42 | 0.46 | 0.62 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.4 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6531 | 1.923 | 1.6572 | 1.5719 | 1.0686 | 1.6545 | -0.5791 | 0.1669 | 0.2465 | 0.0691 | -0.3519 | 0.1211 | 3.6549 | 4.5324 | 3.726 | 2.7958 | 3.5736 | 3.425 |
Actin | 0.0662 | 0.0133 | 0.0266 | 0.0069 | 0.0045 | 0.0285 | 0.0261 | 0.014 | 0.0282 | 0.0127 | 0.0028 | 0.0243 | 0.0245 | 0.0828 | 0.0096 | 0.0297 | 0.0384 | 0.041 |
Bud | 0.0032 | 0.007 | 0.0065 | 0.0007 | 0.0011 | 0.0005 | 0.0044 | 0.0074 | 0.0071 | 0.0024 | 0.0004 | 0.0035 | 0.0013 | 0.0022 | 0.002 | 0.0024 | 0.003 | 0.002 |
Bud Neck | 0.1777 | 0.0232 | 0.2216 | 0.6749 | 0.8487 | 0.7679 | 0.1284 | 0.0215 | 0.1177 | 0.6385 | 0.8324 | 0.5783 | 0.0958 | 0.0079 | 0.1457 | 0.6218 | 0.6675 | 0.6426 |
Bud Periphery | 0.0073 | 0.0117 | 0.0142 | 0.0007 | 0.0022 | 0.0011 | 0.0052 | 0.0084 | 0.0084 | 0.0034 | 0.0009 | 0.0075 | 0.0016 | 0.0037 | 0.002 | 0.004 | 0.0021 | 0.0076 |
Bud Site | 0.1982 | 0.2486 | 0.1114 | 0.0072 | 0.0152 | 0.0029 | 0.1968 | 0.2312 | 0.0552 | 0.0241 | 0.0224 | 0.0066 | 0.2919 | 0.1861 | 0.1413 | 0.0604 | 0.0069 | 0.0053 |
Cell Periphery | 0.0117 | 0.0097 | 0.007 | 0.0012 | 0.001 | 0.002 | 0.0043 | 0.0033 | 0.0017 | 0.001 | 0.0006 | 0.0013 | 0.0046 | 0.0072 | 0.0056 | 0.0054 | 0.0017 | 0.0029 |
Cytoplasm | 0.1947 | 0.4534 | 0.3938 | 0.1187 | 0.1089 | 0.1288 | 0.3004 | 0.4508 | 0.4677 | 0.1496 | 0.0452 | 0.2133 | 0.0641 | 0.3327 | 0.326 | 0.1348 | 0.0577 | 0.1786 |
Cytoplasmic Foci | 0.0404 | 0.0183 | 0.0219 | 0.0316 | 0.0099 | 0.009 | 0.0552 | 0.0189 | 0.0357 | 0.0461 | 0.0136 | 0.0399 | 0.116 | 0.0116 | 0.0181 | 0.0327 | 0.0434 | 0.045 |
Eisosomes | 0.0016 | 0.0009 | 0.0006 | 0.0001 | 0 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0022 | 0.0019 | 0.0011 | 0.0008 | 0.0007 | 0.0003 |
Endoplasmic Reticulum | 0.008 | 0.0034 | 0.0063 | 0.0009 | 0.0004 | 0.0007 | 0.0052 | 0.0028 | 0.0051 | 0.0038 | 0.0038 | 0.0024 | 0.0021 | 0.0027 | 0.007 | 0.0009 | 0.001 | 0.0012 |
Endosome | 0.025 | 0.0027 | 0.0179 | 0.0268 | 0.0012 | 0.0076 | 0.0189 | 0.0079 | 0.0157 | 0.016 | 0.0096 | 0.0092 | 0.0103 | 0.0012 | 0.0112 | 0.0008 | 0.0011 | 0.0013 |
Golgi | 0.0096 | 0.0011 | 0.0093 | 0.006 | 0.0006 | 0.0045 | 0.0041 | 0.002 | 0.0032 | 0.0048 | 0.0006 | 0.0032 | 0.003 | 0.0014 | 0.0122 | 0.0013 | 0.0025 | 0.001 |
Lipid Particles | 0.0207 | 0.0007 | 0.0045 | 0.0031 | 0.0001 | 0.0006 | 0.0025 | 0.0006 | 0.0014 | 0.0038 | 0.0002 | 0.0016 | 0.0173 | 0.0008 | 0.0028 | 0.0005 | 0.0219 | 0.0002 |
Mitochondria | 0.0237 | 0.0007 | 0.0071 | 0.0038 | 0.0001 | 0.0039 | 0.0017 | 0.0007 | 0.0078 | 0.0066 | 0.0007 | 0.0015 | 0.0015 | 0.0072 | 0.0053 | 0.0002 | 0.0009 | 0.0002 |
None | 0.1492 | 0.1913 | 0.1303 | 0.0891 | 0.0034 | 0.0244 | 0.2129 | 0.2163 | 0.2185 | 0.0534 | 0.0289 | 0.0923 | 0.3166 | 0.3432 | 0.2962 | 0.0856 | 0.0724 | 0.062 |
Nuclear Periphery | 0.0163 | 0.0006 | 0.0052 | 0.0001 | 0.0001 | 0.0034 | 0.0064 | 0.0009 | 0.0026 | 0.0113 | 0.0276 | 0.0035 | 0.0034 | 0.0007 | 0.0007 | 0.0002 | 0.0002 | 0.0002 |
Nucleolus | 0.0021 | 0.0006 | 0.0008 | 0.0003 | 0.0002 | 0.0005 | 0.0031 | 0.0009 | 0.0011 | 0.0007 | 0.0007 | 0.0006 | 0.0044 | 0.0005 | 0.0004 | 0.0007 | 0.0007 | 0.0001 |
Nucleus | 0.0081 | 0.0042 | 0.0025 | 0.0005 | 0.0011 | 0.0099 | 0.0099 | 0.0044 | 0.0059 | 0.0037 | 0.0051 | 0.0027 | 0.0052 | 0.0011 | 0.0012 | 0.0014 | 0.0013 | 0.0005 |
Peroxisomes | 0.0147 | 0.0005 | 0.003 | 0.0202 | 0.0002 | 0.0013 | 0.0023 | 0.0006 | 0.0069 | 0.005 | 0.0003 | 0.0029 | 0.0098 | 0.0028 | 0.0074 | 0.0013 | 0.0721 | 0.0068 |
Punctate Nuclear | 0.0113 | 0.0013 | 0.0019 | 0.0004 | 0.0006 | 0.0006 | 0.0049 | 0.002 | 0.0032 | 0.0049 | 0.0014 | 0.0022 | 0.0211 | 0.0012 | 0.0009 | 0.0139 | 0.0033 | 0.0007 |
Vacuole | 0.0072 | 0.0066 | 0.0061 | 0.0062 | 0.0005 | 0.0009 | 0.0061 | 0.0049 | 0.0058 | 0.0059 | 0.0018 | 0.0021 | 0.0027 | 0.001 | 0.003 | 0.001 | 0.001 | 0.0005 |
Vacuole Periphery | 0.0029 | 0.0004 | 0.0016 | 0.0006 | 0 | 0.0006 | 0.0007 | 0.0003 | 0.0007 | 0.0019 | 0.001 | 0.0005 | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.2373 | 13.3735 | 17.185 | 36.2563 | 19.8696 | 16.7753 | 17.2322 | 22.816 | 28.4672 | 26.5184 |
Translational Efficiency | 0.6286 | 0.6712 | 0.8311 | 0.478 | 0.4982 | 0.9419 | 0.6262 | 0.4222 | 0.563 | 0.5666 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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