Standard name
Human Ortholog
Description Arf3p polarization-specific docking factor; required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0.1 0.07 0.07 0.08 0.09 0.11
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0.06 0.06 0.05
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 – 0.08 0.06 0.08 0.07 0.14 0.12 0.19 0.16
Cytoplasm 0.94 0.94 0.99 0.97 0.99 0.96 0.95 – 0.84 0.84 0.67 0.88 0.99 0.99 1.0 – 0.9 0.94 0.54 0.66 0.59 0.47 0.38 0.42
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.06 0 0.13 0 0 0.06 0.07 0.08
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.09 – 0.23 0.21 0.41 0.27 0 0 0 – 0 0 0 0.06 0.11 0.1 0.09 0.07
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 1 0 0 0 1 – 0 1 0 0 0 0 0 – 4 0 33 24 24 28 42 45
Bud 9 2 0 0 1 0 2 – 4 6 5 3 5 1 0 – 0 0 2 1 2 11 21 18
Bud Neck 1 1 15 6 10 7 12 – 5 5 5 10 0 0 0 – 3 1 9 9 4 18 26 20
Bud Site 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 16 9 15 9 15 13 8 – 11 5 6 0 13 26 16 – 26 16 28 27 50 41 87 66
Cytoplasm 316 324 376 261 364 358 427 – 312 247 154 165 336 463 384 – 278 248 184 240 217 156 177 166
Endoplasmic Reticulum 5 13 1 0 0 0 1 – 2 2 3 0 0 0 2 – 18 9 43 15 5 20 34 31
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 2 1 5 2 1 0 1 2
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 1 1 3 2 0 1 1 0
Mitochondria 6 4 0 4 5 24 41 – 85 62 93 50 2 1 0 – 7 1 13 23 41 33 44 26
Nucleus 0 0 1 2 0 3 1 – 1 1 2 2 1 0 0 – 3 2 0 1 0 0 0 0
Nuclear Periphery 0 1 0 1 0 1 0 – 0 0 0 0 0 0 0 – 0 0 1 0 0 1 1 0
Nucleolus 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 – 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 1 0 5 – 5 3 5 3 0 1 0 – 4 2 1 1 2 0 2 3
Vac/Vac Membrane 0 1 1 0 2 0 1 – 0 1 0 0 0 2 0 – 14 4 6 12 4 14 19 13
Unique Cell Count 335 346 379 268 368 372 448 370 293 229 188 339 467 385 308 265 343 367 368 335 469 401
Labelled Cell Count 356 358 410 284 398 406 500 425 333 274 234 357 494 403 361 285 343 367 368 335 469 401


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 4.7 5.0 4.6 4.9 3.9 4.0 – 3.4 3.3 3.0 3.6 4.3 4.6 4.4 – 7.1 6.1 4.4 4.7 5.3
Std Deviation (1e-4) 0.6 0.8 1.0 1.4 1.3 1.1 1.2 – 1.1 1.0 0.8 1.0 1.1 1.5 1.3 – 1.6 1.3 1.3 1.3 1.8
Intensity Change (Log2) – – – -0.11 -0.04 -0.38 -0.33 – -0.55 -0.61 -0.74 -0.49 -0.23 -0.12 -0.19 – 0.49 0.28 -0.18 -0.1 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 3.8 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8331 2.3099 2.0949 1.0627 2.487 1.8166 0.2229 0.3237 0.1825 0.2684 -0.2039 0.2999 -0.2283 0.4014 -0.0545 0.1015 0.1707 -0.1026
Actin 0.0543 0.0063 0.0168 0.0097 0.0108 0.0052 0.0413 0.009 0.0165 0.0069 0.004 0.0084 0.0158 0.0007 0.0045 0.0017 0.0087 0.0055
Bud 0.0009 0.0011 0.0012 0.0005 0.0004 0.0002 0.0015 0.0013 0.0018 0.0035 0.0006 0.0005 0.0085 0.0503 0.0319 0.01 0.0057 0.0134
Bud Neck 0.005 0.001 0.0021 0.0051 0.0038 0.0128 0.0117 0.0055 0.0069 0.0024 0.0033 0.004 0.0041 0.0013 0.0018 0.0103 0.0008 0.0056
Bud Periphery 0.003 0.0021 0.0035 0.0011 0.0005 0.0013 0.0041 0.003 0.0064 0.0202 0.0011 0.002 0.0083 0.0129 0.0192 0.0095 0.0056 0.0138
Bud Site 0.0079 0.0378 0.0116 0.0199 0.0076 0.0004 0.0375 0.0484 0.0205 0.0115 0.0009 0.0006 0.0356 0.1241 0.0443 0.0206 0.0004 0.0051
Cell Periphery 0.0062 0.014 0.0061 0.0063 0.0011 0.0083 0.005 0.0119 0.0104 0.0102 0.001 0.0037 0.0037 0.0071 0.0025 0.0018 0.0008 0.0014
Cytoplasm 0.1388 0.2504 0.1589 0.3121 0.2562 0.3294 0.2326 0.4201 0.3654 0.2791 0.3904 0.4737 0.1222 0.1939 0.1772 0.2126 0.2571 0.3056
Cytoplasmic Foci 0.0327 0.0057 0.0112 0.0376 0.0479 0.0057 0.0385 0.0053 0.0122 0.0096 0.055 0.0099 0.023 0.004 0.0129 0.0055 0.012 0.0149
Eisosomes 0.0022 0.0008 0.001 0.0004 0.0001 0.0006 0.0007 0.0006 0.0006 0.0004 0.0002 0.0002 0.0005 0.0002 0.0004 0.0003 0.0003 0.0001
Endoplasmic Reticulum 0.0077 0.0023 0.003 0.007 0.0021 0.0023 0.0039 0.0038 0.0039 0.0064 0.0099 0.0028 0.0041 0.0016 0.0018 0.0027 0.0027 0.002
Endosome 0.0243 0.0002 0.0065 0.0101 0.0674 0.0062 0.0336 0.0005 0.0089 0.0075 0.0285 0.0029 0.0074 0.0002 0.0027 0.0008 0.0025 0.0006
Golgi 0.0141 0.0002 0.0071 0.0112 0.0201 0.0039 0.0155 0.0004 0.0032 0.004 0.0129 0.0029 0.0024 0.0001 0.0026 0.0003 0.0015 0.0004
Lipid Particles 0.0219 0.0005 0.0022 0.009 0.0894 0.0016 0.0279 0.0007 0.0042 0.0225 0.0074 0.0013 0.0063 0.0002 0.0045 0.0009 0.0305 0.0004
Mitochondria 0.0305 0.0002 0.0034 0.006 0.0751 0.002 0.0101 0.0003 0.0159 0.0042 0.001 0.0024 0.0109 0.0004 0.0041 0.0014 0.0008 0.0013
None 0.5835 0.6738 0.756 0.5115 0.3252 0.6177 0.4922 0.4852 0.5112 0.5883 0.4665 0.479 0.7036 0.5733 0.6388 0.6676 0.6034 0.6044
Nuclear Periphery 0.0053 0.0002 0.0003 0.0004 0.0061 0.0002 0.0031 0.0003 0.0006 0.0013 0.0029 0.0003 0.0044 0.0009 0.0024 0.0018 0.0034 0.002
Nucleolus 0.0034 0.0003 0.0004 0.0003 0.0005 0.0001 0.0018 0.0003 0.0005 0.001 0.0005 0.0001 0.002 0.0018 0.0041 0.0193 0.0022 0.0009
Nucleus 0.0074 0.0005 0.0007 0.0006 0.0009 0.0003 0.0025 0.0009 0.0012 0.0026 0.0018 0.0006 0.0069 0.015 0.0192 0.0149 0.012 0.0071
Peroxisomes 0.0295 0.0013 0.0047 0.049 0.0675 0.0006 0.0172 0.0006 0.006 0.0133 0.0045 0.0032 0.007 0.0001 0.0045 0.0006 0.029 0.0025
Punctate Nuclear 0.0157 0.0003 0.0006 0.0009 0.0035 0.0002 0.0125 0.001 0.0014 0.0008 0.0046 0.0007 0.0208 0.0101 0.0174 0.0144 0.0195 0.0113
Vacuole 0.0035 0.0009 0.0025 0.0011 0.0081 0.0007 0.0053 0.0011 0.002 0.0036 0.0024 0.0008 0.0023 0.0016 0.0028 0.0027 0.0008 0.0014
Vacuole Periphery 0.0021 0 0.0002 0.0002 0.0058 0.0001 0.0013 0 0.0003 0.0006 0.0004 0.0001 0.0004 0.0001 0.0005 0.0003 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.4339 11.0333 8.733 8.9817 8.8013 4.523 11.0628 10.9176 9.4327 9.4052
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Arf3p polarization-specific docking factor; required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p
Localization
Cell Percentages cytoplasm (19%), ER (7%), mixed (68%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Afi1

Afi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Afi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available