Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.95 0.95 0.9 0.94 0.88 0.85 0.78 0.73 0.7 0.6 0.66 0.98 0.98 1.0 0.99 0.96 0.96 0.86 0.95 0.88 0.9 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.09 0 0.08 0.17 0.19 0.36 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.05 0 0.13 0.15 0.15 0.13 0.14 0.17 0 0 0 0 0 0 0.08 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 1 1
Bud 0 2 0 0 0 1 1 0 1 0 0 1 0 0 0 0 0 0 0 0 1 1 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 0 0 1 0 0 6 3 1 5 2 4 0 1 0 4 0 2 0 0 0 0 0 0
Cytoplasm 144 158 41 96 118 81 145 97 98 120 71 99 141 260 303 134 104 129 151 179 64 122 216 199
Endoplasmic Reticulum 0 3 0 0 1 1 2 0 1 4 0 2 0 0 0 7 3 5 0 0 0 1 1 1
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 1 1 1 0 0 0 0 0
Mitochondria 3 8 1 3 2 8 4 10 23 32 43 33 1 0 0 0 0 1 2 3 0 2 0 1
Nucleus 4 0 1 3 1 2 1 5 4 4 0 3 1 2 1 1 1 2 1 0 0 2 2 4
Nuclear Periphery 4 0 0 1 0 4 1 0 1 2 1 2 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0
Vac/Vac Membrane 4 0 0 8 6 3 22 19 20 23 17 25 1 3 3 1 3 6 14 1 0 1 0 2
Unique Cell Count 153 167 43 107 126 92 171 124 134 171 118 151 144 264 304 135 108 135 177 189 73 136 234 225
Labelled Cell Count 161 171 43 112 128 101 182 136 149 190 134 171 144 268 308 147 112 147 177 189 73 136 234 225


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 4.5 7.3 7.4 9.7 7.9 9.4 8.9 8.4 8.9 8.3 8.9 8.3 9.0 9.8 9.7 12.3 11.5 9.8 11.5 13.3
Std Deviation (1e-4) 1.8 1.0 1.9 1.8 2.4 2.4 2.3 2.2 2.6 2.6 2.4 3.3 2.0 2.0 2.4 3.1 4.9 3.7 3.0 3.5 3.9
Intensity Change (Log2) 0.03 0.41 0.12 0.37 0.29 0.21 0.28 0.2 0.29 0.19 0.31 0.42 0.42 0.76 0.65 0.43 0.66 0.87


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0123 2.3485 1.2508 1.4099 0.5908 1.5872 4.4302 5.6977 4.5119 4.4388 4.8033 4.9605 2.1135 1.8858 1.8992 2.3166 2.1317 2.1098
Actin 0.0306 0.0003 0.0234 0.0002 0.0024 0.0083 0.0162 0.0128 0.0036 0.0003 0.0005 0.0021 0.0338 0.0006 0.0108 0.0165 0.0453 0.004
Bud 0.001 0.0007 0.0029 0.0003 0.0014 0.0011 0.0017 0.0008 0.0024 0.0003 0.0002 0.0006 0.0009 0.0005 0.0092 0.0004 0.0003 0.0003
Bud Neck 0.001 0.0003 0.0055 0.0003 0.0005 0.0014 0.0007 0.0002 0.0007 0.0002 0.0003 0.0011 0.0088 0.0001 0.0019 0.0003 0.0002 0.0004
Bud Periphery 0.001 0.0004 0.0034 0.0001 0.0008 0.0013 0.0038 0.0006 0.0013 0.0002 0.0001 0.0005 0.0011 0.0006 0.0046 0.0003 0.0003 0.0008
Bud Site 0.0042 0.0012 0.0044 0.0001 0.0003 0.0015 0.0009 0.0029 0.0029 0.0001 0.0001 0.0018 0.0148 0.001 0.0078 0.0004 0.0005 0.0001
Cell Periphery 0.0003 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0 0.0001 0.0004 0.0002 0.0003 0.0001 0 0.0001
Cytoplasm 0.7092 0.7823 0.6698 0.8298 0.7752 0.8053 0.5824 0.6777 0.6327 0.73 0.6415 0.6596 0.4082 0.5159 0.5126 0.5448 0.5879 0.6444
Cytoplasmic Foci 0.0237 0.0071 0.0228 0.0053 0.0265 0.0189 0.0178 0.0054 0.0086 0.0041 0.0089 0.0058 0.0285 0.0031 0.0187 0.0164 0.0091 0.0146
Eisosomes 0.0006 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0003 0.0001 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0059 0.0078 0.0057 0.0025 0.0057 0.0031 0.0031 0.0014 0.0014 0.0012 0.0016 0.0012 0.0054 0.0011 0.0028 0.0047 0.0007 0.002
Endosome 0.0151 0.0047 0.017 0.003 0.0122 0.0071 0.0044 0.0008 0.0014 0.0014 0.0027 0.0018 0.0225 0.0004 0.0043 0.009 0.0006 0.0045
Golgi 0.0055 0.0002 0.0063 0.0001 0.0006 0.0018 0.0014 0.0002 0.0007 0.0001 0.0002 0.0004 0.0103 0.0001 0.0017 0.0031 0.0004 0.0008
Lipid Particles 0.0108 0.0002 0.0021 0.0001 0.0009 0.0011 0.0009 0.0001 0.0003 0 0.0009 0.0002 0.0084 0.0001 0.0034 0.0011 0.0001 0.0005
Mitochondria 0.0062 0.0002 0.0097 0.0001 0.0004 0.0006 0.0005 0.0002 0.0004 0.0001 0.0002 0.0002 0.0029 0.0002 0.0104 0.0014 0.0002 0.0005
None 0.1491 0.182 0.2052 0.1531 0.1534 0.1256 0.3477 0.2931 0.3361 0.2591 0.3365 0.3146 0.4111 0.4735 0.3974 0.3523 0.3515 0.3106
Nuclear Periphery 0.0072 0.0012 0.0041 0.001 0.0034 0.0026 0.002 0.0003 0.0006 0.0004 0.0008 0.0005 0.0068 0.0005 0.0011 0.0012 0.0002 0.0063
Nucleolus 0.0009 0.0002 0.0004 0 0.0001 0.0003 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0018 0.0001 0.0006 0 0 0
Nucleus 0.0058 0.0073 0.0055 0.0022 0.0079 0.0096 0.0023 0.0015 0.0028 0.0012 0.0018 0.0026 0.0086 0.0009 0.0025 0.0018 0.0006 0.0084
Peroxisomes 0.0129 0.0001 0.0043 0 0.0005 0.004 0.0019 0.0005 0.0025 0.0001 0.0002 0.0048 0.0065 0.0001 0.0072 0.0446 0.0015 0.0004
Punctate Nuclear 0.0066 0.0013 0.004 0.0003 0.0023 0.0039 0.0107 0.0004 0.0007 0.0003 0.0019 0.0013 0.0166 0.0006 0.0013 0.0008 0.0003 0.0007
Vacuole 0.0018 0.0022 0.0024 0.0012 0.0047 0.0021 0.0008 0.001 0.0006 0.0006 0.001 0.0004 0.0017 0.0005 0.0009 0.0006 0.0003 0.0004
Vacuole Periphery 0.0008 0.0003 0.0007 0.0002 0.0007 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0006 0.0001 0.0001 0.0002 0 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.9012 9.8885 10.0893 23.9753 9.8601 20.7292 19.0591 23.6185 27.079 21.833
Translational Efficiency 2.1618 3.6862 3.4585 1.2994 3.2183 3.5448 2.6591 2.6673 2.4956 2.4702

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rcn2

Rcn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rcn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available