Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 0 | 1 | 0 | 0 | 6 | 3 | 1 | 5 | 2 | 4 | 0 | 1 | 0 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 144 | 158 | 41 | 96 | 118 | 81 | 145 | 97 | 98 | 120 | 71 | 99 | 141 | 260 | 303 | 134 | 104 | 129 | 151 | 179 | 64 | 122 | 216 | 199 |
Endoplasmic Reticulum | 0 | 3 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 4 | 0 | 2 | 0 | 0 | 0 | 7 | 3 | 5 | 0 | 0 | 0 | 1 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 8 | 1 | 3 | 2 | 8 | 4 | 10 | 23 | 32 | 43 | 33 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 2 | 0 | 1 |
Nucleus | 4 | 0 | 1 | 3 | 1 | 2 | 1 | 5 | 4 | 4 | 0 | 3 | 1 | 2 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 2 | 2 | 4 |
Nuclear Periphery | 4 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 |
Vac/Vac Membrane | 4 | 0 | 0 | 8 | 6 | 3 | 22 | 19 | 20 | 23 | 17 | 25 | 1 | 3 | 3 | 1 | 3 | 6 | 14 | 1 | 0 | 1 | 0 | 2 |
Unique Cell Count | 153 | 167 | 43 | 107 | 126 | 92 | 171 | 124 | 134 | 171 | 118 | 151 | 144 | 264 | 304 | 135 | 108 | 135 | 177 | 189 | 73 | 136 | 234 | 225 |
Labelled Cell Count | 161 | 171 | 43 | 112 | 128 | 101 | 182 | 136 | 149 | 190 | 134 | 171 | 144 | 268 | 308 | 147 | 112 | 147 | 177 | 189 | 73 | 136 | 234 | 225 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 4.5 | 7.3 | 7.4 | 9.7 | 7.9 | 9.4 | 8.9 | 8.4 | 8.9 | 8.3 | 8.9 | 8.3 | 9.0 | 9.8 | 9.7 | 12.3 | 11.5 | 9.8 | 11.5 | 13.3 |
Std Deviation (1e-4) | 1.8 | 1.0 | 1.9 | 1.8 | 2.4 | 2.4 | 2.3 | 2.2 | 2.6 | 2.6 | 2.4 | 3.3 | 2.0 | 2.0 | 2.4 | 3.1 | 4.9 | 3.7 | 3.0 | 3.5 | 3.9 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.41 | 0.12 | 0.37 | 0.29 | 0.21 | 0.28 | 0.2 | 0.29 | 0.19 | 0.31 | 0.42 | 0.42 | 0.76 | 0.65 | 0.43 | 0.66 | 0.87 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0123 | 2.3485 | 1.2508 | 1.4099 | 0.5908 | 1.5872 | 4.4302 | 5.6977 | 4.5119 | 4.4388 | 4.8033 | 4.9605 | 2.1135 | 1.8858 | 1.8992 | 2.3166 | 2.1317 | 2.1098 |
Actin | 0.0306 | 0.0003 | 0.0234 | 0.0002 | 0.0024 | 0.0083 | 0.0162 | 0.0128 | 0.0036 | 0.0003 | 0.0005 | 0.0021 | 0.0338 | 0.0006 | 0.0108 | 0.0165 | 0.0453 | 0.004 |
Bud | 0.001 | 0.0007 | 0.0029 | 0.0003 | 0.0014 | 0.0011 | 0.0017 | 0.0008 | 0.0024 | 0.0003 | 0.0002 | 0.0006 | 0.0009 | 0.0005 | 0.0092 | 0.0004 | 0.0003 | 0.0003 |
Bud Neck | 0.001 | 0.0003 | 0.0055 | 0.0003 | 0.0005 | 0.0014 | 0.0007 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0011 | 0.0088 | 0.0001 | 0.0019 | 0.0003 | 0.0002 | 0.0004 |
Bud Periphery | 0.001 | 0.0004 | 0.0034 | 0.0001 | 0.0008 | 0.0013 | 0.0038 | 0.0006 | 0.0013 | 0.0002 | 0.0001 | 0.0005 | 0.0011 | 0.0006 | 0.0046 | 0.0003 | 0.0003 | 0.0008 |
Bud Site | 0.0042 | 0.0012 | 0.0044 | 0.0001 | 0.0003 | 0.0015 | 0.0009 | 0.0029 | 0.0029 | 0.0001 | 0.0001 | 0.0018 | 0.0148 | 0.001 | 0.0078 | 0.0004 | 0.0005 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.7092 | 0.7823 | 0.6698 | 0.8298 | 0.7752 | 0.8053 | 0.5824 | 0.6777 | 0.6327 | 0.73 | 0.6415 | 0.6596 | 0.4082 | 0.5159 | 0.5126 | 0.5448 | 0.5879 | 0.6444 |
Cytoplasmic Foci | 0.0237 | 0.0071 | 0.0228 | 0.0053 | 0.0265 | 0.0189 | 0.0178 | 0.0054 | 0.0086 | 0.0041 | 0.0089 | 0.0058 | 0.0285 | 0.0031 | 0.0187 | 0.0164 | 0.0091 | 0.0146 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0059 | 0.0078 | 0.0057 | 0.0025 | 0.0057 | 0.0031 | 0.0031 | 0.0014 | 0.0014 | 0.0012 | 0.0016 | 0.0012 | 0.0054 | 0.0011 | 0.0028 | 0.0047 | 0.0007 | 0.002 |
Endosome | 0.0151 | 0.0047 | 0.017 | 0.003 | 0.0122 | 0.0071 | 0.0044 | 0.0008 | 0.0014 | 0.0014 | 0.0027 | 0.0018 | 0.0225 | 0.0004 | 0.0043 | 0.009 | 0.0006 | 0.0045 |
Golgi | 0.0055 | 0.0002 | 0.0063 | 0.0001 | 0.0006 | 0.0018 | 0.0014 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0103 | 0.0001 | 0.0017 | 0.0031 | 0.0004 | 0.0008 |
Lipid Particles | 0.0108 | 0.0002 | 0.0021 | 0.0001 | 0.0009 | 0.0011 | 0.0009 | 0.0001 | 0.0003 | 0 | 0.0009 | 0.0002 | 0.0084 | 0.0001 | 0.0034 | 0.0011 | 0.0001 | 0.0005 |
Mitochondria | 0.0062 | 0.0002 | 0.0097 | 0.0001 | 0.0004 | 0.0006 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0029 | 0.0002 | 0.0104 | 0.0014 | 0.0002 | 0.0005 |
None | 0.1491 | 0.182 | 0.2052 | 0.1531 | 0.1534 | 0.1256 | 0.3477 | 0.2931 | 0.3361 | 0.2591 | 0.3365 | 0.3146 | 0.4111 | 0.4735 | 0.3974 | 0.3523 | 0.3515 | 0.3106 |
Nuclear Periphery | 0.0072 | 0.0012 | 0.0041 | 0.001 | 0.0034 | 0.0026 | 0.002 | 0.0003 | 0.0006 | 0.0004 | 0.0008 | 0.0005 | 0.0068 | 0.0005 | 0.0011 | 0.0012 | 0.0002 | 0.0063 |
Nucleolus | 0.0009 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0018 | 0.0001 | 0.0006 | 0 | 0 | 0 |
Nucleus | 0.0058 | 0.0073 | 0.0055 | 0.0022 | 0.0079 | 0.0096 | 0.0023 | 0.0015 | 0.0028 | 0.0012 | 0.0018 | 0.0026 | 0.0086 | 0.0009 | 0.0025 | 0.0018 | 0.0006 | 0.0084 |
Peroxisomes | 0.0129 | 0.0001 | 0.0043 | 0 | 0.0005 | 0.004 | 0.0019 | 0.0005 | 0.0025 | 0.0001 | 0.0002 | 0.0048 | 0.0065 | 0.0001 | 0.0072 | 0.0446 | 0.0015 | 0.0004 |
Punctate Nuclear | 0.0066 | 0.0013 | 0.004 | 0.0003 | 0.0023 | 0.0039 | 0.0107 | 0.0004 | 0.0007 | 0.0003 | 0.0019 | 0.0013 | 0.0166 | 0.0006 | 0.0013 | 0.0008 | 0.0003 | 0.0007 |
Vacuole | 0.0018 | 0.0022 | 0.0024 | 0.0012 | 0.0047 | 0.0021 | 0.0008 | 0.001 | 0.0006 | 0.0006 | 0.001 | 0.0004 | 0.0017 | 0.0005 | 0.0009 | 0.0006 | 0.0003 | 0.0004 |
Vacuole Periphery | 0.0008 | 0.0003 | 0.0007 | 0.0002 | 0.0007 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.9012 | 9.8885 | 10.0893 | 23.9753 | 9.8601 | 20.7292 | 19.0591 | 23.6185 | 27.079 | 21.833 |
Translational Efficiency | 2.1618 | 3.6862 | 3.4585 | 1.2994 | 3.2183 | 3.5448 | 2.6591 | 2.6673 | 2.4956 | 2.4702 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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