Standard name
Human Ortholog
Description Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.15 0.14 0.22 0.18 0.16 0.11 0.11 0.08 0.07 0.05 0 0.41 0.39 0.46 0.31 0.27 0.25 0 0.12 0.05 0.1 0.14 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.1 0 0.18 0.13 0.32 0.5 0.33 0.38 0.54 0.54 0.59 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.56 0.44 0.54 0.34 0.39 0.43 0.42 0.44 0.48 0.5 0.54 0.47 0.43 0.31 0.22 0.41 0.37 0.36 0.68 0.58 0.57 0.53 0.4 0.28
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.32 0.48 0.41 0.4 0.45 0.3 0.22 0.24 0.23 0.17 0.17 0.19 0.41 0.5 0.48 0.31 0.41 0.44 0.23 0.2 0.26 0.3 0.33 0.35
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 0 1 1
Bud 0 0 0 1 0 3 13 9 5 14 6 7 0 0 0 0 0 0 3 6 7 4 7 12
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 5
Bud Site 0 0 0 0 0 2 7 4 5 18 9 11 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 2 0 0 0 1 0 0 0 1 1 1 1 0 0 0 0 0 0 0
Cytoplasm 19 30 56 55 59 77 75 79 46 47 21 16 122 135 176 86 53 56 8 28 23 33 55 90
Endoplasmic Reticulum 0 2 2 1 0 1 0 0 1 0 0 1 1 3 1 16 11 13 0 0 4 0 3 5
Endosome 2 0 5 2 10 3 1 7 8 0 0 0 0 6 12 4 4 8 5 4 5 3 10 19
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 2 1 0 3
Mitochondria 51 20 10 43 42 158 352 228 217 377 243 314 0 0 0 3 8 3 5 4 11 6 14 12
Nucleus 177 88 212 84 130 208 299 303 270 345 240 251 129 107 85 112 72 80 262 133 244 179 158 130
Nuclear Periphery 3 0 0 0 0 4 2 2 6 5 4 3 0 0 0 1 1 0 1 2 4 0 1 4
Nucleolus 8 1 0 1 0 0 5 12 3 15 10 12 0 0 0 2 0 0 1 0 1 0 2 2
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 2 2 5 4 5 6
Vac/Vac Membrane 103 97 159 97 152 147 156 164 133 119 74 102 121 172 187 86 80 98 89 46 110 101 131 165
Unique Cell Count 317 202 390 245 335 488 707 689 566 693 448 534 298 345 386 274 197 221 387 232 429 339 400 470
Labelled Cell Count 365 239 445 284 394 605 910 808 694 943 607 717 374 424 462 312 232 260 387 232 429 339 400 470


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.5 6.7 6.2 6.1 5.3 5.1 5.4 5.4 5.0 5.0 4.9 7.9 7.9 7.7 8.0 7.5 7.8 8.4 8.6 8.7
Std Deviation (1e-4) 0.8 1.2 1.1 1.7 1.6 1.1 1.1 1.3 1.2 1.2 1.2 1.2 1.0 1.3 1.2 1.7 1.6 1.8 1.3 1.3 1.3
Intensity Change (Log2) -0.12 -0.14 -0.35 -0.4 -0.32 -0.31 -0.43 -0.41 -0.46 0.23 0.25 0.2 0.26 0.17 0.22 0.33 0.36 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.6 1.2 0.6 -1.8 -1.4 -3.1 -4.1 -4.8 -6.4 7.9 7.6 9.5 5.3 3.7 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.2 0 4.1
Endosome 0 1.6 0 0 0 0.2 0 0 0 0 0.5 1.7 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 6.6 5.2 11.2 15.9 11.6 12.8 17.1 16.3 0 0 0 0 -1.3 1.0 0
Nucleus -4.9 -4.2 -3.5 -3.8 -3.3 -2.0 -1.4 -0.2 -2.2 -2.9 -6.4 -9.3 -3.4 -4.1 -4.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2.9 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 1.2 -3.3 -6.6 -5.8 -5.7 -8.5 -7.8 -7.2 0 2.5 2.2 -2.5 0 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.4213 5.9314 5.5634 5.121 5.3157 5.3757 2.1781 2.7957 2.5776 2.3012 2.3525 2.4431 2.6477 3.0108 2.5829 2.7721 2.3326 2.428
Actin 0.0033 0.0016 0.0007 0.0006 0.0017 0.0004 0.0017 0.0001 0.0002 0.0002 0.0009 0.0002 0.0331 0.0002 0.0086 0.0008 0.0078 0.0012
Bud 0.0002 0.0009 0.0002 0.0002 0.0001 0.0002 0.0001 0.0037 0.0004 0.0004 0.0002 0.0009 0.0037 0.0072 0.003 0.0004 0.0102 0.0028
Bud Neck 0.0109 0.0018 0.0007 0.0005 0.0011 0.003 0.0005 0.0083 0.0005 0.0014 0.0021 0.0032 0.012 0.0009 0.0009 0.0012 0.0026 0.0047
Bud Periphery 0.0001 0.0011 0.0002 0.0001 0.0001 0.0001 0.0002 0.0005 0.0001 0.0002 0.0002 0.0004 0.0013 0.0019 0.0013 0.0003 0.0037 0.0018
Bud Site 0.0009 0.0059 0.001 0.0004 0.0005 0.0003 0.0004 0.0025 0.0008 0.0002 0.0003 0.0002 0.0063 0.0064 0.0024 0.0011 0.0032 0.0008
Cell Periphery 0.0001 0.0006 0.0001 0 0.0001 0 0.0001 0.0001 0 0 0 0 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001
Cytoplasm 0.2056 0.2931 0.1524 0.2033 0.2483 0.1827 0.1267 0.1964 0.1283 0.1704 0.1076 0.1369 0.1514 0.1433 0.1278 0.1107 0.1146 0.1432
Cytoplasmic Foci 0.0078 0.0064 0.0031 0.0034 0.0051 0.0057 0.0014 0.004 0.0032 0.004 0.0013 0.0016 0.039 0.0021 0.0047 0.0027 0.0603 0.0054
Eisosomes 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0005 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0074 0.0075 0.0041 0.0049 0.0046 0.0043 0.0046 0.0026 0.0017 0.0027 0.0015 0.002 0.0136 0.0032 0.0042 0.0043 0.003 0.0031
Endosome 0.0102 0.0128 0.0089 0.0043 0.0082 0.0092 0.0047 0.006 0.0026 0.0122 0.0029 0.0039 0.0414 0.0023 0.0091 0.0061 0.0221 0.0054
Golgi 0.0015 0.0009 0.0003 0.0002 0.0006 0.0002 0.0004 0.0001 0.0001 0.0002 0.0001 0 0.0131 0 0.0003 0.0006 0.0053 0.0036
Lipid Particles 0.0023 0.0011 0.0004 0.0001 0.0011 0.0003 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0218 0.0001 0.0004 0.0017 0.0314 0.0028
Mitochondria 0.001 0.0242 0.0016 0.0011 0.0008 0.0008 0.0018 0.0006 0.0009 0.0016 0.0006 0.0005 0.0054 0.0011 0.0015 0.0556 0.0027 0.0027
None 0.0618 0.0444 0.054 0.0178 0.0223 0.0135 0.0098 0.0195 0.0063 0.0042 0.0246 0.0036 0.0621 0.0372 0.0186 0.0032 0.0109 0.0136
Nuclear Periphery 0.0197 0.0184 0.0217 0.0155 0.0259 0.0158 0.0269 0.0202 0.016 0.0157 0.0151 0.0141 0.0387 0.0204 0.0233 0.0386 0.0148 0.0142
Nucleolus 0.0024 0.0035 0.0039 0.0019 0.0024 0.0047 0.0014 0.0024 0.0032 0.0021 0.0031 0.0024 0.0034 0.0028 0.0027 0.0024 0.0025 0.0044
Nucleus 0.6357 0.5351 0.7296 0.7364 0.6277 0.7109 0.8039 0.7167 0.809 0.755 0.8102 0.8141 0.493 0.7456 0.7621 0.6997 0.6297 0.7244
Peroxisomes 0.001 0.0018 0.0004 0.0001 0.0014 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0124 0.0001 0.0007 0.0013 0.0215 0.0055
Punctate Nuclear 0.024 0.0326 0.0117 0.0074 0.0441 0.0418 0.0109 0.0118 0.0245 0.0265 0.0229 0.0133 0.0406 0.0181 0.0242 0.0653 0.047 0.0555
Vacuole 0.0036 0.005 0.004 0.0015 0.0034 0.0053 0.0034 0.0036 0.0016 0.0018 0.0053 0.0023 0.0051 0.0056 0.0034 0.0027 0.0056 0.0038
Vacuole Periphery 0.0006 0.0011 0.0009 0.0004 0.0005 0.0007 0.0009 0.0006 0.0005 0.0007 0.0009 0.0005 0.0018 0.0012 0.0007 0.001 0.0008 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.8595 6.8977 10.8055 12.4395 5.0021 6.855 7.7492 10.1989 11.057 11.5775
Translational Efficiency 1.2419 1.4902 1.3933 1.2339 2.0646 1.5718 1.6508 1.2752 1.233 1.0831

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects
Localization
Cell Percentages nucleus (71%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mod5

Mod5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mod5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available