Standard name | |
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Human Ortholog | |
Description | Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 3 | 13 | 9 | 5 | 14 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 7 | 4 | 7 | 12 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 7 | 4 | 5 | 18 | 9 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 19 | 30 | 56 | 55 | 59 | 77 | 75 | 79 | 46 | 47 | 21 | 16 | 122 | 135 | 176 | 86 | 53 | 56 | 8 | 28 | 23 | 33 | 55 | 90 |
Endoplasmic Reticulum | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 16 | 11 | 13 | 0 | 0 | 4 | 0 | 3 | 5 |
Endosome | 2 | 0 | 5 | 2 | 10 | 3 | 1 | 7 | 8 | 0 | 0 | 0 | 0 | 6 | 12 | 4 | 4 | 8 | 5 | 4 | 5 | 3 | 10 | 19 |
Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 2 | 1 | 0 | 3 |
Mitochondria | 51 | 20 | 10 | 43 | 42 | 158 | 352 | 228 | 217 | 377 | 243 | 314 | 0 | 0 | 0 | 3 | 8 | 3 | 5 | 4 | 11 | 6 | 14 | 12 |
Nucleus | 177 | 88 | 212 | 84 | 130 | 208 | 299 | 303 | 270 | 345 | 240 | 251 | 129 | 107 | 85 | 112 | 72 | 80 | 262 | 133 | 244 | 179 | 158 | 130 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 0 | 4 | 2 | 2 | 6 | 5 | 4 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 4 | 0 | 1 | 4 |
Nucleolus | 8 | 1 | 0 | 1 | 0 | 0 | 5 | 12 | 3 | 15 | 10 | 12 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 2 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 4 | 5 | 6 |
Vac/Vac Membrane | 103 | 97 | 159 | 97 | 152 | 147 | 156 | 164 | 133 | 119 | 74 | 102 | 121 | 172 | 187 | 86 | 80 | 98 | 89 | 46 | 110 | 101 | 131 | 165 |
Unique Cell Count | 317 | 202 | 390 | 245 | 335 | 488 | 707 | 689 | 566 | 693 | 448 | 534 | 298 | 345 | 386 | 274 | 197 | 221 | 387 | 232 | 429 | 339 | 400 | 470 |
Labelled Cell Count | 365 | 239 | 445 | 284 | 394 | 605 | 910 | 808 | 694 | 943 | 607 | 717 | 374 | 424 | 462 | 312 | 232 | 260 | 387 | 232 | 429 | 339 | 400 | 470 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 6.5 | 6.7 | 6.2 | 6.1 | 5.3 | 5.1 | 5.4 | 5.4 | 5.0 | 5.0 | 4.9 | 7.9 | 7.9 | 7.7 | 8.0 | 7.5 | 7.8 | 8.4 | 8.6 | 8.7 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.1 | 1.7 | 1.6 | 1.1 | 1.1 | 1.3 | 1.2 | 1.2 | 1.2 | 1.2 | 1.0 | 1.3 | 1.2 | 1.7 | 1.6 | 1.8 | 1.3 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.14 | -0.35 | -0.4 | -0.32 | -0.31 | -0.43 | -0.41 | -0.46 | 0.23 | 0.25 | 0.2 | 0.26 | 0.17 | 0.22 | 0.33 | 0.36 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.6 | 1.2 | 0.6 | -1.8 | -1.4 | -3.1 | -4.1 | -4.8 | -6.4 | 7.9 | 7.6 | 9.5 | 5.3 | 3.7 | 3.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 4.1 |
Endosome | 0 | 1.6 | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0.5 | 1.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6.6 | 5.2 | 11.2 | 15.9 | 11.6 | 12.8 | 17.1 | 16.3 | 0 | 0 | 0 | 0 | -1.3 | 1.0 | 0 |
Nucleus | -4.9 | -4.2 | -3.5 | -3.8 | -3.3 | -2.0 | -1.4 | -0.2 | -2.2 | -2.9 | -6.4 | -9.3 | -3.4 | -4.1 | -4.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.3 | 1.2 | -3.3 | -6.6 | -5.8 | -5.7 | -8.5 | -7.8 | -7.2 | 0 | 2.5 | 2.2 | -2.5 | 0 | 0.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.4213 | 5.9314 | 5.5634 | 5.121 | 5.3157 | 5.3757 | 2.1781 | 2.7957 | 2.5776 | 2.3012 | 2.3525 | 2.4431 | 2.6477 | 3.0108 | 2.5829 | 2.7721 | 2.3326 | 2.428 |
Actin | 0.0033 | 0.0016 | 0.0007 | 0.0006 | 0.0017 | 0.0004 | 0.0017 | 0.0001 | 0.0002 | 0.0002 | 0.0009 | 0.0002 | 0.0331 | 0.0002 | 0.0086 | 0.0008 | 0.0078 | 0.0012 |
Bud | 0.0002 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0037 | 0.0004 | 0.0004 | 0.0002 | 0.0009 | 0.0037 | 0.0072 | 0.003 | 0.0004 | 0.0102 | 0.0028 |
Bud Neck | 0.0109 | 0.0018 | 0.0007 | 0.0005 | 0.0011 | 0.003 | 0.0005 | 0.0083 | 0.0005 | 0.0014 | 0.0021 | 0.0032 | 0.012 | 0.0009 | 0.0009 | 0.0012 | 0.0026 | 0.0047 |
Bud Periphery | 0.0001 | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0013 | 0.0019 | 0.0013 | 0.0003 | 0.0037 | 0.0018 |
Bud Site | 0.0009 | 0.0059 | 0.001 | 0.0004 | 0.0005 | 0.0003 | 0.0004 | 0.0025 | 0.0008 | 0.0002 | 0.0003 | 0.0002 | 0.0063 | 0.0064 | 0.0024 | 0.0011 | 0.0032 | 0.0008 |
Cell Periphery | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.2056 | 0.2931 | 0.1524 | 0.2033 | 0.2483 | 0.1827 | 0.1267 | 0.1964 | 0.1283 | 0.1704 | 0.1076 | 0.1369 | 0.1514 | 0.1433 | 0.1278 | 0.1107 | 0.1146 | 0.1432 |
Cytoplasmic Foci | 0.0078 | 0.0064 | 0.0031 | 0.0034 | 0.0051 | 0.0057 | 0.0014 | 0.004 | 0.0032 | 0.004 | 0.0013 | 0.0016 | 0.039 | 0.0021 | 0.0047 | 0.0027 | 0.0603 | 0.0054 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0074 | 0.0075 | 0.0041 | 0.0049 | 0.0046 | 0.0043 | 0.0046 | 0.0026 | 0.0017 | 0.0027 | 0.0015 | 0.002 | 0.0136 | 0.0032 | 0.0042 | 0.0043 | 0.003 | 0.0031 |
Endosome | 0.0102 | 0.0128 | 0.0089 | 0.0043 | 0.0082 | 0.0092 | 0.0047 | 0.006 | 0.0026 | 0.0122 | 0.0029 | 0.0039 | 0.0414 | 0.0023 | 0.0091 | 0.0061 | 0.0221 | 0.0054 |
Golgi | 0.0015 | 0.0009 | 0.0003 | 0.0002 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0131 | 0 | 0.0003 | 0.0006 | 0.0053 | 0.0036 |
Lipid Particles | 0.0023 | 0.0011 | 0.0004 | 0.0001 | 0.0011 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0218 | 0.0001 | 0.0004 | 0.0017 | 0.0314 | 0.0028 |
Mitochondria | 0.001 | 0.0242 | 0.0016 | 0.0011 | 0.0008 | 0.0008 | 0.0018 | 0.0006 | 0.0009 | 0.0016 | 0.0006 | 0.0005 | 0.0054 | 0.0011 | 0.0015 | 0.0556 | 0.0027 | 0.0027 |
None | 0.0618 | 0.0444 | 0.054 | 0.0178 | 0.0223 | 0.0135 | 0.0098 | 0.0195 | 0.0063 | 0.0042 | 0.0246 | 0.0036 | 0.0621 | 0.0372 | 0.0186 | 0.0032 | 0.0109 | 0.0136 |
Nuclear Periphery | 0.0197 | 0.0184 | 0.0217 | 0.0155 | 0.0259 | 0.0158 | 0.0269 | 0.0202 | 0.016 | 0.0157 | 0.0151 | 0.0141 | 0.0387 | 0.0204 | 0.0233 | 0.0386 | 0.0148 | 0.0142 |
Nucleolus | 0.0024 | 0.0035 | 0.0039 | 0.0019 | 0.0024 | 0.0047 | 0.0014 | 0.0024 | 0.0032 | 0.0021 | 0.0031 | 0.0024 | 0.0034 | 0.0028 | 0.0027 | 0.0024 | 0.0025 | 0.0044 |
Nucleus | 0.6357 | 0.5351 | 0.7296 | 0.7364 | 0.6277 | 0.7109 | 0.8039 | 0.7167 | 0.809 | 0.755 | 0.8102 | 0.8141 | 0.493 | 0.7456 | 0.7621 | 0.6997 | 0.6297 | 0.7244 |
Peroxisomes | 0.001 | 0.0018 | 0.0004 | 0.0001 | 0.0014 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0124 | 0.0001 | 0.0007 | 0.0013 | 0.0215 | 0.0055 |
Punctate Nuclear | 0.024 | 0.0326 | 0.0117 | 0.0074 | 0.0441 | 0.0418 | 0.0109 | 0.0118 | 0.0245 | 0.0265 | 0.0229 | 0.0133 | 0.0406 | 0.0181 | 0.0242 | 0.0653 | 0.047 | 0.0555 |
Vacuole | 0.0036 | 0.005 | 0.004 | 0.0015 | 0.0034 | 0.0053 | 0.0034 | 0.0036 | 0.0016 | 0.0018 | 0.0053 | 0.0023 | 0.0051 | 0.0056 | 0.0034 | 0.0027 | 0.0056 | 0.0038 |
Vacuole Periphery | 0.0006 | 0.0011 | 0.0009 | 0.0004 | 0.0005 | 0.0007 | 0.0009 | 0.0006 | 0.0005 | 0.0007 | 0.0009 | 0.0005 | 0.0018 | 0.0012 | 0.0007 | 0.001 | 0.0008 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.8595 | 6.8977 | 10.8055 | 12.4395 | 5.0021 | 6.855 | 7.7492 | 10.1989 | 11.057 | 11.5775 |
Translational Efficiency | 1.2419 | 1.4902 | 1.3933 | 1.2339 | 2.0646 | 1.5718 | 1.6508 | 1.2752 | 1.233 | 1.0831 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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