Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 1 | 2 | 2 | 1 | 2 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 4 | 3 | 8 | 8 | 6 | 8 | 3 | 9 | 1 | 1 | 3 | 2 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 81 | 16 | – | 144 | 286 | 170 | 222 | 222 | 136 | 123 | 103 | 151 | 154 | 179 | 192 | 230 | 200 | 248 | 71 | 40 | 20 | 61 | 135 | 164 |
Endoplasmic Reticulum | 0 | 1 | – | 3 | 10 | 4 | 29 | 48 | 31 | 17 | 25 | 31 | 7 | 0 | 1 | 5 | 7 | 9 | 5 | 1 | 0 | 5 | 12 | 25 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 5 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 0 | – | 0 | 0 | 1 | 0 | 3 | 0 | 2 | 1 | 3 | 0 | 2 | 0 | 1 | 3 | 1 | 0 | 1 | 0 | 4 | 0 | 3 |
Unique Cell Count | 81 | 18 | 146 | 295 | 177 | 255 | 277 | 165 | 147 | 135 | 198 | 159 | 181 | 193 | 233 | 208 | 256 | 79 | 47 | 24 | 75 | 154 | 200 | |
Labelled Cell Count | 81 | 20 | 147 | 301 | 180 | 263 | 285 | 177 | 153 | 140 | 208 | 162 | 182 | 198 | 238 | 217 | 268 | 79 | 47 | 24 | 75 | 154 | 200 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 93.7 | 77.3 | – | 87.1 | 70.0 | 56.5 | 50.0 | 47.8 | 45.3 | 41.3 | 40.6 | 40.4 | 90.9 | 91.5 | 90.3 | 142.8 | 145.7 | 143.2 | 86.6 | 88.2 | 95.7 |
Std Deviation (1e-4) | 16.0 | 27.4 | – | 17.0 | 13.9 | 11.6 | 11.5 | 11.7 | 10.6 | 10.2 | 11.1 | 10.8 | 20.3 | 22.0 | 20.6 | 39.0 | 39.1 | 42.7 | 14.9 | 20.8 | 23.9 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 62.1388 | 110.7212 | 96.9045 | 62.7445 | 87.1795 | 87.6773 | 99.8048 | 124.3057 | 111.2874 | 122.818 | 107.5973 | 102.0187 | 96.7663 | 131.3147 | 114.8711 | 121.2984 | 32.6527 | 121.3378 |
Actin | 0.025 | 0.0004 | 0.0017 | 0.0043 | 0.0033 | 0.0017 | 0.0054 | 0.0007 | 0.0032 | 0.0005 | 0.003 | 0.002 | 0.0106 | 0.0007 | 0.0026 | 0.001 | 0.0446 | 0.0047 |
Bud | 0.0074 | 0.0007 | 0.002 | 0.049 | 0.0008 | 0.0034 | 0.0027 | 0.0013 | 0.0034 | 0.0018 | 0.0067 | 0.0013 | 0.0035 | 0.0022 | 0.0073 | 0.003 | 0.0487 | 0.0116 |
Bud Neck | 0.0014 | 0.0005 | 0.0005 | 0.0018 | 0.0013 | 0.0032 | 0.0007 | 0.0006 | 0.0011 | 0.0003 | 0.0017 | 0.0018 | 0.0011 | 0.0015 | 0.0013 | 0.0019 | 0.0016 | 0.0011 |
Bud Periphery | 0.0177 | 0.0012 | 0.0013 | 0.0711 | 0.0019 | 0.0027 | 0.0035 | 0.0008 | 0.0034 | 0.0005 | 0.0041 | 0.0013 | 0.0038 | 0.0027 | 0.0063 | 0.0016 | 0.0724 | 0.0056 |
Bud Site | 0.0025 | 0.0006 | 0.001 | 0.0093 | 0.0002 | 0.0008 | 0.0011 | 0.0013 | 0.0016 | 0.0003 | 0.001 | 0.0003 | 0.0016 | 0.0018 | 0.0043 | 0.0011 | 0.0208 | 0.0006 |
Cell Periphery | 0.0339 | 0.0206 | 0.0092 | 0.0114 | 0.0154 | 0.0068 | 0.0313 | 0.0133 | 0.0173 | 0.0325 | 0.0089 | 0.0209 | 0.0219 | 0.0492 | 0.0343 | 0.088 | 0.0039 | 0.0028 |
Cytoplasm | 0.4419 | 0.8839 | 0.799 | 0.469 | 0.4924 | 0.6457 | 0.6201 | 0.8691 | 0.7969 | 0.69 | 0.5979 | 0.5984 | 0.5832 | 0.7162 | 0.6683 | 0.4018 | 0.2397 | 0.7758 |
Cytoplasmic Foci | 0.0091 | 0.0015 | 0.0029 | 0.0037 | 0.004 | 0.0038 | 0.0017 | 0.002 | 0.0047 | 0.001 | 0.0017 | 0.0084 | 0.0251 | 0.0024 | 0.015 | 0.0053 | 0.0363 | 0.0054 |
Eisosomes | 0.0007 | 0.0002 | 0 | 0 | 0.0061 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0994 | 0.0112 | 0.0065 | 0.0122 | 0.1572 | 0.1136 | 0.0633 | 0.0179 | 0.0093 | 0.0006 | 0.005 | 0.0759 | 0.0564 | 0.0245 | 0.0242 | 0.006 | 0.0142 | 0.0184 |
Endosome | 0.0385 | 0.0032 | 0.0093 | 0.018 | 0.0577 | 0.0271 | 0.0155 | 0.0043 | 0.0152 | 0.0033 | 0.0135 | 0.0665 | 0.0166 | 0.0079 | 0.0152 | 0.023 | 0.1944 | 0.0303 |
Golgi | 0.0084 | 0.0007 | 0.0016 | 0.002 | 0.049 | 0.0027 | 0.0038 | 0.0013 | 0.0035 | 0.0001 | 0.0027 | 0.0265 | 0.0045 | 0.0015 | 0.0024 | 0.0011 | 0.0118 | 0.0064 |
Lipid Particles | 0.006 | 0.0011 | 0.0009 | 0.0011 | 0.0004 | 0.0023 | 0.0012 | 0.0008 | 0.0039 | 0.0004 | 0.0006 | 0.0033 | 0.0062 | 0.0024 | 0.0059 | 0.0046 | 0.0015 | 0.0004 |
Mitochondria | 0.0452 | 0.0043 | 0.0012 | 0.0104 | 0.1555 | 0.0047 | 0.0128 | 0.0166 | 0.0055 | 0.0001 | 0.0551 | 0.0654 | 0.0088 | 0.0012 | 0.008 | 0.001 | 0.011 | 0.0108 |
None | 0.003 | 0.0005 | 0.0004 | 0.0007 | 0.0008 | 0.0029 | 0.0011 | 0.0003 | 0.0025 | 0.0001 | 0.0005 | 0.0011 | 0.0121 | 0.0003 | 0.0143 | 0.0006 | 0.0087 | 0.0009 |
Nuclear Periphery | 0.0977 | 0.0052 | 0.0058 | 0.0135 | 0.0082 | 0.0271 | 0.0528 | 0.0034 | 0.012 | 0.0028 | 0.0152 | 0.0187 | 0.0373 | 0.0063 | 0.0135 | 0.0123 | 0.0207 | 0.0131 |
Nucleolus | 0.0007 | 0.0021 | 0.0006 | 0.0017 | 0.0001 | 0.0078 | 0.0004 | 0.0012 | 0.0008 | 0.001 | 0.0005 | 0.0052 | 0.0021 | 0.0012 | 0.0029 | 0.0019 | 0.001 | 0.0003 |
Nucleus | 0.0159 | 0.0042 | 0.0031 | 0.0105 | 0.0012 | 0.0268 | 0.0095 | 0.0022 | 0.0048 | 0.0046 | 0.0065 | 0.0112 | 0.0095 | 0.0038 | 0.0056 | 0.0059 | 0.0126 | 0.0067 |
Peroxisomes | 0.0068 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0049 | 0.0001 | 0.0008 | 0.0003 | 0.0016 | 0.0002 |
Punctate Nuclear | 0.0026 | 0 | 0.0001 | 0.0002 | 0 | 0.0025 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0011 | 0 | 0.0007 | 0.0001 | 0.0019 | 0.0003 |
Vacuole | 0.0967 | 0.0511 | 0.1225 | 0.2284 | 0.0264 | 0.0872 | 0.144 | 0.057 | 0.0874 | 0.2339 | 0.1849 | 0.0471 | 0.1448 | 0.1517 | 0.1324 | 0.3457 | 0.1886 | 0.0587 |
Vacuole Periphery | 0.0395 | 0.0067 | 0.0302 | 0.0811 | 0.0181 | 0.0268 | 0.0287 | 0.0056 | 0.0222 | 0.0259 | 0.09 | 0.044 | 0.0447 | 0.0223 | 0.0344 | 0.0938 | 0.0638 | 0.046 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1848.5745 | 1853.0188 | 1960.2848 | 1998.8584 | 1922.6434 | 1776.3689 | 1722.7378 | 1586.192 | 1546.622 | 1651.4789 |
Translational Efficiency | 1.7235 | 1.8088 | 1.7999 | 1.3608 | 1.8641 | 1.8813 | 1.6619 | 1.7863 | 1.7929 | 2.0399 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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