Standard name | |
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Human Ortholog | |
Description | N-acetylglucosaminyltransferase; capable of modification of N-linked glycans in the Golgi apparatus |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 |
Bud | 0 | 3 | 1 | 2 | 0 | 2 | 2 | 6 | – | 3 | 14 | 7 | 0 | 0 | 0 | – | 0 | 1 | 1 | 2 | 2 | 4 | 0 | 3 |
Bud Neck | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 118 | 483 | 249 | 206 | 210 | 245 | 356 | 522 | – | 384 | 367 | 382 | 137 | 202 | 261 | – | 324 | 275 | 46 | 200 | 58 | 71 | 80 | 103 |
Endoplasmic Reticulum | 1 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 32 | 20 | 1 | 0 | 7 | 6 | 3 | 4 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | – | 1 | 1 | 0 | 0 | 0 | 0 | – | 7 | 13 | 10 | 9 | 3 | 3 | 4 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 20 | 22 | 13 | 7 | 8 | 47 | 89 | 85 | – | 87 | 105 | 128 | 0 | 0 | 0 | – | 17 | 11 | 8 | 9 | 18 | 8 | 8 | 14 |
Nucleus | 2 | 0 | 0 | 0 | 1 | 2 | 2 | 4 | – | 1 | 1 | 0 | 0 | 3 | 2 | – | 10 | 22 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 1 | 1 | 0 | 5 | 4 | 1 | – | 2 | 2 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 3 | 4 | 2 | 2 | 0 | 1 | 1 |
Vac/Vac Membrane | 28 | 25 | 30 | 8 | 52 | 54 | 48 | 45 | – | 13 | 16 | 18 | 0 | 0 | 0 | – | 118 | 151 | 144 | 296 | 172 | 97 | 118 | 121 |
Unique Cell Count | 155 | 515 | 276 | 212 | 242 | 295 | 409 | 582 | 417 | 406 | 436 | 138 | 205 | 262 | 411 | 416 | 224 | 535 | 277 | 200 | 226 | 265 | ||
Labelled Cell Count | 172 | 537 | 295 | 226 | 273 | 357 | 504 | 670 | 491 | 509 | 538 | 141 | 207 | 264 | 509 | 497 | 224 | 535 | 277 | 200 | 226 | 265 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 3.8 | 3.6 | 3.8 | 3.8 | 3.4 | 3.1 | 3.6 | – | 2.8 | 3.1 | 3.0 | 5.2 | 5.2 | 5.2 | 4.4 | 5.2 | 5.6 | 6.1 | 5.5 | 5.8 |
Std Deviation (1e-4) | 0.7 | 0.6 | 0.9 | 1.2 | 0.8 | 1.1 | 0.8 | 1.2 | – | 0.4 | 1.2 | 0.8 | 0.9 | 1.1 | 1.1 | 1.3 | 1.6 | 1.6 | 10.5 | 1.8 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.09 | -0.07 | -0.22 | -0.02 | – | -0.37 | -0.2 | -0.28 | 0.51 | 0.53 | 0.54 | 0.3 | 0.52 | 0.63 | 0.76 | 0.6 | 0.69 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 5.6 | 7.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0434 | 0.8747 | 0.9191 | 0.6737 | 0.9617 | 1.1745 | 2.136 | 2.3917 | 2.9179 | 2.2818 | 2.788 | 2.6313 | 8.5412 | 10.1076 | 8.5743 | 9.5246 | 13.7033 | 11.9873 |
Actin | 0.0383 | 0.002 | 0.0033 | 0.0005 | 0.0152 | 0.0081 | 0.02 | 0.0001 | 0.0091 | 0.0026 | 0.0248 | 0.0027 | 0.001 | 0.001 | 0.0025 | 0.0019 | 0.009 | 0.0026 |
Bud | 0.0014 | 0.001 | 0.0078 | 0.0004 | 0.0029 | 0.0021 | 0.0035 | 0.0042 | 0.0018 | 0.0295 | 0.0061 | 0.0014 | 0.0243 | 0.0417 | 0.0347 | 0.0355 | 0.0042 | 0.0208 |
Bud Neck | 0.0043 | 0.0023 | 0.0033 | 0.0004 | 0.0043 | 0.0022 | 0.0016 | 0.0011 | 0.0058 | 0.0164 | 0.0024 | 0.0056 | 0.0007 | 0.0006 | 0.0008 | 0.0012 | 0.0015 | 0.0033 |
Bud Periphery | 0.0039 | 0.0021 | 0.0042 | 0.0005 | 0.0079 | 0.0048 | 0.0131 | 0.004 | 0.0033 | 0.0113 | 0.0204 | 0.0015 | 0.1072 | 0.0767 | 0.104 | 0.1074 | 0.0424 | 0.104 |
Bud Site | 0.0072 | 0.008 | 0.0087 | 0.0003 | 0.0182 | 0.0013 | 0.0049 | 0.0091 | 0.0076 | 0.0248 | 0.0047 | 0.002 | 0.0199 | 0.0426 | 0.0194 | 0.0107 | 0.0009 | 0.0032 |
Cell Periphery | 0.0016 | 0.0008 | 0.001 | 0.0002 | 0.0013 | 0.0007 | 0.0069 | 0.0012 | 0.0077 | 0.0016 | 0.002 | 0.0006 | 0.2232 | 0.2019 | 0.182 | 0.1723 | 0.2634 | 0.2476 |
Cytoplasm | 0.1047 | 0.232 | 0.1287 | 0.1229 | 0.1193 | 0.1931 | 0.1418 | 0.1828 | 0.1565 | 0.1127 | 0.0831 | 0.1918 | 0.0968 | 0.1519 | 0.1049 | 0.0931 | 0.1024 | 0.1526 |
Cytoplasmic Foci | 0.0591 | 0.0345 | 0.0958 | 0.0832 | 0.095 | 0.0426 | 0.067 | 0.044 | 0.0628 | 0.0709 | 0.1187 | 0.0625 | 0.0333 | 0.0198 | 0.0345 | 0.0619 | 0.046 | 0.0274 |
Eisosomes | 0.0005 | 0.0002 | 0.004 | 0.0001 | 0.0006 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0007 | 0.0003 | 0.0003 | 0.0018 | 0.0004 |
Endoplasmic Reticulum | 0.0083 | 0.0042 | 0.0024 | 0.0027 | 0.0046 | 0.0035 | 0.0093 | 0.0036 | 0.0037 | 0.0051 | 0.0059 | 0.003 | 0.0501 | 0.0211 | 0.0217 | 0.0677 | 0.1031 | 0.0537 |
Endosome | 0.0954 | 0.0911 | 0.1155 | 0.2822 | 0.145 | 0.1024 | 0.1145 | 0.1194 | 0.1229 | 0.2118 | 0.2351 | 0.1378 | 0.0447 | 0.0595 | 0.0759 | 0.0664 | 0.0204 | 0.0523 |
Golgi | 0.0072 | 0.0017 | 0.0042 | 0.0124 | 0.0075 | 0.0055 | 0.0076 | 0.0017 | 0.0066 | 0.0048 | 0.0171 | 0.0046 | 0.0007 | 0.001 | 0.0013 | 0.0014 | 0.007 | 0.0007 |
Lipid Particles | 0.0171 | 0.0038 | 0.0217 | 0.003 | 0.0178 | 0.0082 | 0.014 | 0.004 | 0.0066 | 0.0085 | 0.0227 | 0.0043 | 0.0056 | 0.0079 | 0.0032 | 0.0058 | 0.0033 | 0.0036 |
Mitochondria | 0.0232 | 0.0023 | 0.0131 | 0.0072 | 0.0233 | 0.0111 | 0.0149 | 0.0036 | 0.0165 | 0.0162 | 0.0225 | 0.0061 | 0.0037 | 0.0062 | 0.0036 | 0.0125 | 0.0013 | 0.0022 |
None | 0.4939 | 0.4742 | 0.4769 | 0.3764 | 0.3864 | 0.4478 | 0.4524 | 0.3882 | 0.4458 | 0.2478 | 0.1913 | 0.3736 | 0.2883 | 0.2468 | 0.3007 | 0.2422 | 0.3601 | 0.2223 |
Nuclear Periphery | 0.0063 | 0.0032 | 0.0023 | 0.0022 | 0.0083 | 0.0086 | 0.0055 | 0.0037 | 0.0035 | 0.0051 | 0.0094 | 0.0028 | 0.0086 | 0.0069 | 0.0042 | 0.0053 | 0.0051 | 0.0058 |
Nucleolus | 0.0044 | 0.0039 | 0.0062 | 0.0009 | 0.0134 | 0.0031 | 0.0022 | 0.0031 | 0.0025 | 0.0044 | 0.0023 | 0.0019 | 0.002 | 0.0019 | 0.0026 | 0.0039 | 0.0008 | 0.0012 |
Nucleus | 0.0069 | 0.0187 | 0.0067 | 0.0027 | 0.0118 | 0.0116 | 0.0046 | 0.0093 | 0.0064 | 0.0116 | 0.0063 | 0.0096 | 0.0033 | 0.0088 | 0.0058 | 0.0048 | 0.0013 | 0.0053 |
Peroxisomes | 0.0169 | 0.0006 | 0.0145 | 0.0015 | 0.0209 | 0.0024 | 0.0117 | 0.0007 | 0.0055 | 0.0058 | 0.0149 | 0.0073 | 0.0006 | 0.0007 | 0.0011 | 0.0117 | 0.0003 | 0.0005 |
Punctate Nuclear | 0.0178 | 0.0021 | 0.0035 | 0.0011 | 0.0073 | 0.0024 | 0.0045 | 0.0027 | 0.0034 | 0.0047 | 0.0317 | 0.0023 | 0.0013 | 0.0037 | 0.0041 | 0.0097 | 0.0015 | 0.0025 |
Vacuole | 0.0741 | 0.1057 | 0.0688 | 0.0875 | 0.0822 | 0.1279 | 0.0909 | 0.1995 | 0.1098 | 0.1844 | 0.1519 | 0.1661 | 0.0793 | 0.092 | 0.0842 | 0.0782 | 0.023 | 0.0811 |
Vacuole Periphery | 0.0074 | 0.0057 | 0.0075 | 0.0118 | 0.0067 | 0.0105 | 0.0079 | 0.0138 | 0.0121 | 0.02 | 0.0264 | 0.0126 | 0.0048 | 0.0064 | 0.0086 | 0.0062 | 0.0012 | 0.007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.6111 | 40.0966 | 29.7534 | 44.3269 | 42.1623 | 42.5876 | 44.9336 | 42.1844 | 42.6115 | 61.8023 |
Translational Efficiency | 0.8916 | 0.5645 | 0.7438 | 0.4721 | 0.5393 | 0.785 | 0.6083 | 0.5578 | 0.588 | 0.4455 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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