Standard name
Human Ortholog
Description Cytoplasmic and mitochondrial alanyl-tRNA synthetase; required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog AARS; mutations in human homolog AARS are associated with autoimmune disease polymyositis/dermatomyositis
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.05 0.06 – 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.99 0.98 0.96 0.9 0.88 0.88 – 0.84 0.85 0.98 0.99 0.99 0.98 1.0 1.0 0.99 0.94 0.96 0.96 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0.1 0.08 – 0.12 0.12 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 1 2 7 – 7 9 0 0 0 0 0 0 0 0 0 1 0 0
Bud 2 0 0 3 0 0 0 0 1 – 5 1 0 1 0 0 0 1 0 0 2 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 4 7 9 13 32 21 23 – 21 27 1 1 5 4 1 3 0 0 0 0 0 0
Cytoplasm 249 96 319 363 435 371 538 400 337 – 303 377 241 394 407 361 202 205 234 108 309 164 208 304
Endoplasmic Reticulum 0 0 6 2 13 16 53 46 29 – 43 51 4 1 0 18 12 16 0 2 5 1 9 23
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 1 2 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 1 – 0 2 0 0 2 0 0 0 0 1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 0 0 3 0 – 0 0 0 2 2 7 0 0 0 0 2 0 2 2
Unique Cell Count 249 97 324 365 446 388 597 457 382 360 443 245 396 412 369 203 205 237 116 322 171 229 338
Labelled Cell Count 253 97 330 375 457 400 624 473 401 380 468 246 399 416 391 215 225 237 116 322 171 229 338


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 111.4 93.9 70.6 67.3 71.2 68.2 68.7 64.0 58.3 – 60.5 57.6 99.3 89.1 74.4 99.7 122.8 121.9 93.0 88.2 98.2
Std Deviation (1e-4) 19.7 23.0 12.6 12.9 13.2 16.2 15.8 15.2 16.0 – 17.8 15.9 18.9 19.6 16.9 32.3 32.7 38.5 22.9 27.8 26.0
Intensity Change (Log2) – – – -0.07 0.01 -0.05 -0.04 -0.14 -0.28 – -0.22 -0.29 0.49 0.33 0.07 0.5 0.8 0.79 0.4 0.32 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0.7 0.8 1.8 3.1 2.6 3.3 – 3.2 3.4 0 0 0 0 0 0
Cytoplasm 1.3 -0.9 -2.2 -4.8 -5.6 -5.3 – -6.5 -6.3 -0.1 1.4 0.4 -0.6 1.1 1.8
Endoplasmic Reticulum 0 0.9 1.7 4.2 4.5 3.5 – 5.1 5.0 0 0 0 2.2 2.5 3.3
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 86.6969 104.7785 97.2078 94.8028 97.0607 101.8782 76.3355 112.9357 104.7483 100.1001 92.5133 87.6259 77.545 97.6648 90.863 68.1838 81.0663 77.2664
Actin 0.0006 0.0002 0.0002 0.0002 0.0003 0.0008 0.006 0.0004 0.003 0.0013 0.0005 0.0087 0.0277 0.0068 0.0003 0.0641 0.0174 0.0022
Bud 0.0006 0.0004 0.0006 0.0008 0.0007 0.0008 0.0022 0.0005 0.001 0.002 0.0005 0.0013 0.0006 0.0004 0.0006 0.0015 0.0067 0.0025
Bud Neck 0.0008 0.001 0.0009 0.0012 0.0015 0.0029 0.001 0.0008 0.0009 0.0008 0.0017 0.0021 0.0007 0.0007 0.0005 0.0016 0.0008 0.0022
Bud Periphery 0.0009 0.0005 0.0005 0.0008 0.0004 0.0011 0.0026 0.0002 0.0006 0.0016 0.0001 0.0017 0.0006 0.0002 0.0004 0.0012 0.0055 0.0011
Bud Site 0.0003 0.0004 0.0003 0.0002 0.0002 0.0002 0.001 0.0005 0.001 0.0007 0.0002 0.0041 0.0025 0.0043 0.0002 0.0068 0.0027 0.0004
Cell Periphery 0.0264 0.0228 0.0231 0.0233 0.0042 0.0108 0.0092 0.0128 0.0062 0.0095 0.0042 0.0016 0.0041 0.0023 0.0029 0.0022 0.0005 0.0008
Cytoplasm 0.6445 0.8619 0.8 0.7138 0.7867 0.828 0.7585 0.9359 0.8935 0.802 0.9458 0.7204 0.8128 0.9652 0.9465 0.7618 0.8276 0.8617
Cytoplasmic Foci 0.0006 0.0003 0.0005 0.0006 0.0014 0.0014 0.0038 0.0005 0.0018 0.002 0.0023 0.0269 0.0025 0.0008 0.0009 0.024 0.0024 0.0129
Eisosomes 0 0 0 0 0 0.0001 0.0001 0 0 0 0 0.001 0.0001 0.0007 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.1026 0.0412 0.0517 0.0295 0.0088 0.0548 0.0228 0.0146 0.006 0.0027 0.0026 0.0467 0.0605 0.0063 0.0064 0.0021 0.0029 0.0288
Endosome 0.0035 0.0006 0.0013 0.002 0.0058 0.0041 0.0099 0.0005 0.0015 0.0285 0.0018 0.0048 0.0091 0.0006 0.0017 0.0047 0.0063 0.0079
Golgi 0.0002 0.0001 0.0002 0.0001 0.0004 0.0005 0.0018 0.0001 0.0003 0.003 0.0001 0.0012 0.0013 0.0002 0.0001 0.0014 0.0012 0.0014
Lipid Particles 0.0012 0.0011 0.0011 0.0006 0.0005 0.0019 0.0012 0.0009 0.0007 0.0007 0.0025 0.0049 0.0042 0.0002 0.0003 0.0061 0.0003 0.0009
Mitochondria 0.0018 0.0002 0.0003 0.0002 0.0019 0.0012 0.0128 0.0001 0.0108 0.0021 0.0002 0.0087 0.0019 0.0003 0.0005 0.0023 0.0598 0.0123
None 0.0009 0.0001 0.0002 0.0002 0.0003 0.0005 0.0033 0.0002 0.0006 0.0002 0.0006 0.0123 0.0021 0.0002 0.0002 0.0055 0.0007 0.0058
Nuclear Periphery 0.0403 0.0075 0.011 0.0106 0.0207 0.0192 0.051 0.0035 0.0047 0.0086 0.0041 0.0177 0.0135 0.0027 0.0022 0.0055 0.0046 0.0044
Nucleolus 0.0011 0.0002 0.0002 0.0003 0.0002 0.0015 0.0014 0.0001 0.0004 0.0003 0.0004 0.0577 0.0003 0.0001 0.0005 0.0082 0.0015 0.0215
Nucleus 0.009 0.0028 0.0032 0.0051 0.0054 0.0052 0.0481 0.0021 0.0033 0.0045 0.005 0.052 0.0035 0.0013 0.0027 0.009 0.0132 0.0129
Peroxisomes 0 0 0 0 0 0 0.0002 0 0.0001 0.0001 0 0.0009 0.0014 0.0001 0 0.0014 0.0009 0.0006
Punctate Nuclear 0.0002 0 0 0.0001 0.0001 0.0001 0.0012 0 0.0002 0.0002 0.0003 0.006 0.0033 0 0 0.0021 0.0003 0.0011
Vacuole 0.1426 0.0551 0.0958 0.1932 0.1356 0.0523 0.0504 0.0255 0.0581 0.1114 0.0251 0.0118 0.0423 0.006 0.0268 0.0681 0.0305 0.0111
Vacuole Periphery 0.0218 0.0035 0.0087 0.0173 0.0252 0.0126 0.0115 0.0007 0.0052 0.0178 0.0021 0.0075 0.0049 0.0008 0.0064 0.02 0.0144 0.0074

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 354.1863 324.335 335.3106 290.7089 305.8138 317.7671 271.1702 247.4213 251.9531 262.6456
Translational Efficiency 1.3009 1.5139 1.3345 1.5681 1.3499 1.3574 1.4671 1.3314 1.306 1.3879

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic and mitochondrial alanyl-tRNA synthetase; required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog AARS; mutations in human homolog AARS are associated with autoimmune disease polymyositis/dermatomyositis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Ala1

Ala1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ala1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available