Standard name
Human Ortholog
Description Putative ortholog of human ETF-dH; found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response; ETF-dH is also known as electron transfer flavoprotein dehydrogenase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.07 0.06 0 0.12 0.06 0.09 0.1 0 0 0 0 0 0.08 0.08 0.09 0.13 0.1 0 0.08 0.08 0.06 0 0
Golgi 0 0 0.28 0.16 0.2 0 0.05 0 0 0 0 0 0.16 0.21 0.26 0.09 0.13 0.07 0 0.06 0.06 0.07 0.1 0.14
Mitochondria 0.92 0.75 0.54 0.85 0.58 0.76 0.71 0.74 0.87 0.87 0.93 0.88 0.9 0.64 0.48 0.15 0.11 0.14 0.83 0.69 0.65 0.72 0.68 0.63
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.08 0.06 0.06 0.07 0.09 0.05 0.07 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0.09 0.05 0.06 0.05 0.07 0 0 0 0 0.11 0.13 0.1 0 0 0 0 0 0
Peroxisomes 0.07 0.12 0.14 0.09 0.11 0.05 0.07 0.06 0 0 0 0 0.05 0.21 0.24 0.13 0.19 0.15 0.07 0.05 0.11 0 0 0
SpindlePole 0 0 0.1 0 0 0 0 0.05 0 0 0 0 0 0.08 0.14 0.23 0.12 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.18 0.2 0.11 0.22 0.15 0.12 0.11 0.06 0.08 0 0.06 0.12 0.05 0.06 0.13 0.14 0.18 0 0 0 0.05 0.08 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 1 4 1 1 1 1 0 0 0 1 1 5 2 1 3 0 3 10 4 2 3 5
Bud 11 6 2 5 4 3 7 15 3 3 6 8 0 10 10 3 4 5 0 5 0 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 3 0 1 0 1 0 3 2 2 1 0 0 0 0 3 2 6 – – – – – –
Cell Periphery 5 10 6 9 5 9 5 2 4 4 2 2 0 3 5 5 7 7 0 0 0 0 0 0
Cytoplasm 2 4 1 2 5 4 7 7 4 2 5 7 0 1 5 2 5 9 0 0 0 0 4 0
Endoplasmic Reticulum 3 1 0 0 0 3 0 1 1 1 0 2 0 0 0 1 3 4 0 0 0 0 0 1
Endosome 10 22 7 8 31 16 30 51 9 9 1 8 2 13 16 5 14 10 4 29 11 7 7 6
Golgi 2 10 34 36 51 12 16 14 2 4 0 9 9 32 52 5 14 7 13 23 9 7 19 29
Mitochondria 303 254 66 188 146 206 241 392 187 222 202 272 52 99 97 8 12 14 278 254 97 81 135 133
Nucleus 3 2 0 0 0 0 10 5 1 3 2 1 0 2 0 3 5 6 0 0 0 0 0 0
Nuclear Periphery 11 8 3 3 5 23 22 33 15 24 11 21 1 1 2 3 3 4 2 0 0 0 1 0
Nucleolus 19 9 4 6 5 12 29 26 13 12 15 13 0 4 1 6 14 10 0 1 0 0 0 0
Peroxisomes 24 42 17 21 27 14 24 32 5 4 0 6 3 33 48 7 21 15 23 16 15 3 5 7
SpindlePole 5 8 12 4 9 7 13 28 4 4 2 10 0 12 27 12 13 16 2 6 4 1 0 0
Vac/Vac Membrane 30 62 25 24 55 42 40 56 13 20 8 19 7 7 12 7 15 19 2 11 3 5 16 16
Unique Cell Count 328 338 123 222 251 272 341 528 214 254 218 308 58 154 200 53 109 103 337 366 150 113 199 210
Labelled Cell Count 429 444 178 311 344 353 445 666 263 314 255 379 75 222 277 71 135 132 337 366 150 113 199 210


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 9.0 9.4 9.1 10.1 8.3 8.5 8.1 7.2 7.4 6.6 7.3 9.4 9.8 9.5 10.2 11.3 10.8 8.6 10.0 10.8
Std Deviation (1e-4) 1.7 2.1 1.5 1.9 2.0 2.1 1.8 1.8 1.8 1.5 1.9 1.7 2.0 1.7 1.7 3.6 3.9 4.0 2.7 2.1 2.2
Intensity Change (Log2) – – – -0.04 0.12 -0.17 -0.13 -0.21 -0.38 -0.34 -0.49 -0.36 0.01 0.07 0.02 0.12 0.27 0.21 -0.12 0.09 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 2.0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 0 0 0 0 0 0 0 0 0 0 0 0 0.5 0.6
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.9 2.0 0.1 1.1 1.4 -0.6 -1.0 0 0 0 0.9 0.8 0 1.9 1.1
Golgi -2.5 -1.6 -6.7 -7.0 -9.6 -7.6 -7.9 -8.2 -7.7 -1.8 -1.3 -0.3 -2.7 -2.8 -4.1
Mitochondria 6.3 0.8 4.4 3.4 4.5 6.9 7.2 8.4 7.9 4.7 1.8 -0.9 -4.8 -6.9 -6.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.2 1.7 1.7 1.8 2.5 1.2 1.8 0 0 0 0 0 0
Nucleolus 0 0 0 1.9 0.8 1.1 0.7 1.4 0 0 0 0 0 2.7 2.0
Peroxisomes -1.2 -0.9 -3.0 -2.3 -2.9 -4.1 -4.9 -5.6 -5.0 -1.7 1.6 2.2 -0.1 1.1 0.2
SpindlePole -3.4 -2.4 -3.1 -2.5 -1.9 -3.3 -3.7 -4.0 -2.8 0 -0.6 1.0 2.3 0.5 1.3
Vacuole -2.4 0.4 -1.2 -2.4 -2.9 -4.0 -3.5 -5.0 -4.4 -1.4 -4.1 -3.9 -1.1 -1.3 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2427 5.3397 4.7454 4.6201 4.3728 4.4512 4.369 5.9091 5.8025 5.2181 5.2075 5.2278 4.2131 5.9424 5.1765 5.4104 5.3931 5.2397
Actin 0.0125 0.0193 0.015 0.0096 0.0103 0.0057 0.0246 0.0266 0.0256 0.0024 0.0087 0.0002 0.033 0.0073 0.013 0.003 0.022 0.0029
Bud 0.0009 0.0018 0.0007 0.001 0.0009 0.0024 0.0009 0.0007 0.0004 0.0008 0.0005 0 0.0006 0.0018 0.0006 0.0007 0.0002 0.0001
Bud Neck 0.0013 0.0005 0.0004 0.0009 0.0005 0.0005 0.0004 0.0004 0.0003 0.0009 0.0002 0 0.0013 0.0003 0.0001 0.0004 0.0001 0.0001
Bud Periphery 0.0021 0.0017 0.0023 0.0019 0.0035 0.0021 0.0021 0.0008 0.0009 0.002 0.001 0 0.0011 0.0033 0.0014 0.001 0.0005 0.0004
Bud Site 0.0049 0.0097 0.0025 0.0114 0.0011 0.0009 0.003 0.0039 0.0009 0.0011 0.0022 0 0.0014 0.0053 0.0029 0.0038 0.0005 0.0002
Cell Periphery 0.0009 0.001 0.0011 0.0006 0.0003 0.0004 0.001 0.0002 0.0003 0.0007 0.0001 0 0.0004 0.0002 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.0028 0.0014 0.0006 0.0017 0.0002 0.0002 0.0032 0.0003 0.0112 0.0003 0.0001 0.026 0.002 0.0006 0.0004 0.0013 0.0001 0.0004
Cytoplasmic Foci 0.02 0.0214 0.0123 0.0168 0.0077 0.0043 0.0288 0.0056 0.0028 0.0042 0.0169 0.0002 0.0226 0.0084 0.0061 0.0241 0.0157 0.0021
Eisosomes 0.0004 0.0006 0.0006 0.0001 0.0003 0.0002 0.0016 0.0003 0.0005 0.0002 0.0002 0.0001 0.0006 0.0002 0.0002 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.0027 0.0016 0.0008 0.001 0.0002 0.0001 0.0031 0.0001 0.0018 0.0001 0.0002 0.0013 0.0044 0.0002 0.0004 0.0004 0.0001 0.0004
Endosome 0.0464 0.0479 0.0307 0.049 0.0393 0.0162 0.0265 0.0204 0.0064 0.0313 0.0092 0.0011 0.0503 0.0176 0.0167 0.0254 0.0053 0.0169
Golgi 0.045 0.0688 0.028 0.0373 0.0304 0.0188 0.0206 0.0236 0.0056 0.0232 0.017 0.0031 0.0351 0.0399 0.015 0.0179 0.018 0.0312
Lipid Particles 0.0582 0.0115 0.0063 0.0473 0.017 0.0267 0.0789 0.0118 0.0141 0.0059 0.0869 0.0026 0.046 0.0078 0.0114 0.0076 0.0161 0.0058
Mitochondria 0.6988 0.75 0.8578 0.7612 0.873 0.9058 0.7064 0.8793 0.8902 0.8766 0.819 0.9583 0.7418 0.8853 0.9043 0.8227 0.8776 0.9155
None 0.0015 0.0004 0.0003 0.0003 0.0001 0.0001 0.0096 0.0001 0.0002 0.0001 0.0003 0.0003 0.0037 0.0001 0.0002 0.0019 0.0002 0.0034
Nuclear Periphery 0.0121 0.001 0.0005 0.0089 0.0001 0.0001 0.0146 0.0001 0.0004 0.0001 0.0005 0.0013 0.0063 0.0001 0.0006 0.0004 0.0001 0.0036
Nucleolus 0.0067 0.0008 0.0002 0.0018 0.0004 0.0005 0.0012 0.0011 0.0002 0.0015 0.0006 0 0.0037 0.0002 0.0001 0.0004 0 0.0003
Nucleus 0.0076 0.0006 0.0003 0.0037 0.0002 0.0003 0.0084 0.0003 0.0004 0.0006 0.0002 0.0025 0.0012 0.0001 0.0002 0.0003 0 0.0005
Peroxisomes 0.0378 0.0192 0.0265 0.0137 0.0082 0.0068 0.0495 0.0155 0.0071 0.0105 0.0298 0.0011 0.028 0.0161 0.0186 0.0449 0.0409 0.0058
Punctate Nuclear 0.0032 0.0002 0.0005 0.0021 0.0001 0.0001 0.0043 0 0 0 0.0015 0.0002 0.0042 0.0002 0.0005 0.0008 0.0011 0.001
Vacuole 0.0208 0.0224 0.0054 0.0184 0.0033 0.0039 0.0054 0.0038 0.0087 0.0255 0.0016 0.0002 0.006 0.002 0.0021 0.0205 0.0003 0.0025
Vacuole Periphery 0.0134 0.0182 0.0072 0.0113 0.0028 0.0039 0.0058 0.005 0.0221 0.0121 0.0033 0.0013 0.0063 0.0029 0.005 0.0223 0.0007 0.0066

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.4425 20.8096 24.7066 25.4886 33.1999 12.7961 16.964 19.3488 27.8906 20.142
Translational Efficiency 0.9951 0.7697 0.7662 1.0409 0.6958 1.5205 0.8925 0.6626 0.6127 0.6704

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative ortholog of human ETF-dH; found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response; ETF-dH is also known as electron transfer flavoprotein dehydrogenase
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Cir2

Cir2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cir2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available