Standard name
Human Ortholog
Description Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.96 0.91 0.82 0.73 0.68 0.61 0.63 0.57 0.61 0.97 0.96 0.98 0.94 0.95 0.95 0.86 0.95 0.82 0.85 0.9 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0.1 0 0.09 0.08 0 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.27 0.28 0.25 0.48 0.49 0.46 0.51 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 1 9 6 5 7 5 0 7 0 1 1 0 0 0 0 0 0 2
Bud 0 0 1 0 0 3 6 9 17 14 5 8 0 2 2 0 1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 2 9 12 18 21 24 14 21 11 9 10 17 13 23 8 7 0 0 0 0 0 1
Cytoplasm 21 103 219 256 370 364 525 598 324 389 205 247 263 536 521 392 227 229 37 104 187 121 355 362
Endoplasmic Reticulum 0 0 3 6 12 14 30 47 20 21 23 14 8 10 4 17 10 10 4 2 21 11 15 35
Endosome 0 0 0 0 1 0 5 22 1 3 3 0 0 7 6 2 4 1 0 0 1 0 1 1
Golgi 0 0 0 1 2 0 1 1 0 1 0 0 0 0 0 1 3 1 0 0 1 0 2 0
Mitochondria 0 3 1 6 26 121 202 223 256 303 164 208 0 1 2 16 5 4 0 0 4 1 3 6
Nucleus 0 0 1 2 0 2 4 4 1 4 0 1 1 0 1 3 3 1 0 0 0 0 1 0
Nuclear Periphery 0 0 1 0 0 0 1 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 9 9 24 55 18 20 12 10 2 4 0 7 4 7 0 1 5 2 3 9
Unique Cell Count 21 104 224 267 407 442 724 875 534 621 358 406 272 557 529 418 238 240 44 110 230 143 394 436
Labelled Cell Count 21 107 229 280 432 533 821 993 658 782 432 503 284 584 550 464 267 260 44 110 230 143 394 436


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 9.5 9.8 9.5 8.7 7.0 6.6 6.7 6.2 6.0 6.1 5.7 10.5 9.8 9.5 10.6 12.6 13.6 10.7 10.2 10.6
Std Deviation (1e-4) 1.1 1.4 1.4 1.7 1.8 1.4 1.4 1.4 1.4 1.2 1.2 1.1 1.5 1.1 1.4 2.2 2.7 3.2 1.7 1.4 1.6
Intensity Change (Log2) -0.04 -0.17 -0.49 -0.57 -0.55 -0.68 -0.72 -0.69 -0.79 0.1 -0.0 -0.05 0.11 0.36 0.47 0.12 0.05 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2.3 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.8 0 2.3 1.7 1.6 0 2.0 1.7 0 2.0 1.8 0 2.9 0 0
Cytoplasm -1.2 -3.3 -5.7 -8.0 -9.0 -10.3 -10.0 -10.7 -10.1 -0.7 -1.1 0.7 -2.2 -1.4 -1.4
Endoplasmic Reticulum 0 1.3 1.4 2.0 2.6 1.8 1.6 2.9 1.6 0 0 0 1.9 1.9 1.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.5 8.5 8.8 8.3 12.6 12.9 11.8 13.0 0 0 0 2.5 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.3 3.5 2.3 2.3 2.3 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6396 5.0089 4.3239 4.051 3.3026 4.2319 3.0319 5.4401 4.6616 4.3687 3.9514 4.5962 6.1744 8.0923 6.9677 6.6574 6.02 6.6289
Actin 0.1218 0.0027 0.0019 0.0011 0.0012 0.0018 0.0453 0.0012 0.0125 0.0006 0.0033 0.0009 0.0051 0.0008 0.0141 0.001 0.0004 0.0018
Bud 0.0021 0.0006 0.0008 0.0005 0.0005 0.0007 0.0005 0.0003 0.0007 0.0005 0.0002 0.0004 0.0003 0.0003 0.0006 0.0006 0.0001 0.0002
Bud Neck 0.0034 0.0005 0.0006 0.0007 0.0024 0.0013 0.0011 0.0001 0.0012 0.0004 0.0003 0.0009 0.0004 0.0002 0.0005 0.0006 0.0004 0.0009
Bud Periphery 0.0031 0.0003 0.0003 0.0002 0.0003 0.0005 0.0005 0.0001 0.0004 0.0001 0.0001 0.0003 0.0003 0.0001 0.0003 0.0002 0 0.0001
Bud Site 0.0099 0.0036 0.0018 0.0004 0.0002 0.0002 0.0022 0.0008 0.0035 0.0003 0.0002 0.0002 0.0009 0.002 0.0028 0.0004 0.0001 0.0001
Cell Periphery 0.0011 0.0012 0.0004 0.0002 0.0002 0.0001 0.0005 0.0001 0.0002 0 0 0 0.0005 0.0004 0.0003 0.0003 0 0.0001
Cytoplasm 0.5642 0.9297 0.8438 0.8493 0.8833 0.8061 0.6391 0.9434 0.8389 0.8305 0.7596 0.8708 0.8345 0.9723 0.9039 0.8614 0.9243 0.9051
Cytoplasmic Foci 0.0373 0.0074 0.0134 0.0207 0.0216 0.0205 0.0535 0.0131 0.0253 0.0629 0.0332 0.0212 0.0173 0.0079 0.0148 0.0262 0.0172 0.0185
Eisosomes 0.0013 0 0 0 0 0 0.001 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0796 0.0256 0.0419 0.0157 0.017 0.0555 0.0232 0.0048 0.0077 0.0049 0.0045 0.0231 0.0511 0.0035 0.016 0.0162 0.0108 0.02
Endosome 0.0637 0.0115 0.0475 0.0877 0.0569 0.0702 0.1009 0.026 0.0666 0.0756 0.0659 0.0495 0.0443 0.0078 0.0294 0.0749 0.0371 0.0342
Golgi 0.0208 0.0024 0.0044 0.0066 0.0029 0.0159 0.0366 0.002 0.0161 0.0059 0.026 0.0073 0.0115 0.0017 0.0081 0.0043 0.0027 0.0064
Lipid Particles 0.0193 0.0002 0.0011 0.0002 0.0001 0.0015 0.0237 0.0001 0.008 0.0002 0.0164 0.0004 0.0016 0.0001 0.0006 0.0002 0.0001 0.0027
Mitochondria 0.0193 0.0006 0.0215 0.0015 0.0008 0.0111 0.0124 0.0004 0.0066 0.0006 0.0031 0.012 0.003 0.0002 0.0014 0.0011 0.0015 0.0019
None 0.0088 0.0019 0.0014 0.0027 0.0006 0.001 0.0328 0.002 0.0026 0.0022 0.0353 0.0018 0.012 0.0008 0.0012 0.0012 0.0012 0.0009
Nuclear Periphery 0.0045 0.0017 0.0027 0.0017 0.0018 0.0027 0.0043 0.0004 0.0011 0.0012 0.0272 0.0008 0.0057 0.0002 0.001 0.0018 0.0008 0.0007
Nucleolus 0.0008 0 0 0 0 0 0.0005 0 0 0 0.0003 0.0003 0.0002 0 0 0 0 0
Nucleus 0.0018 0.0014 0.0013 0.001 0.0015 0.0012 0.0015 0.0004 0.0007 0.001 0.0099 0.0047 0.0019 0.0003 0.0007 0.0011 0.0009 0.0007
Peroxisomes 0.0221 0.0001 0.0001 0.0001 0.0001 0.0006 0.008 0 0.0021 0.0038 0.004 0.0005 0.0002 0.0001 0.0007 0.0001 0.0001 0.0022
Punctate Nuclear 0.0026 0.0001 0.0002 0.0002 0.0001 0.0001 0.0028 0.0001 0.0003 0.0003 0.0051 0.0001 0.0006 0.0001 0.0004 0.0002 0.0001 0.0007
Vacuole 0.0097 0.0074 0.0126 0.007 0.006 0.006 0.0076 0.0038 0.0039 0.007 0.0033 0.0032 0.007 0.001 0.0027 0.0062 0.0016 0.0019
Vacuole Periphery 0.003 0.0011 0.0022 0.0024 0.0025 0.0027 0.002 0.0006 0.0015 0.002 0.0018 0.0015 0.0015 0.0002 0.0007 0.002 0.0007 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.1968 12.5493 25.6084 20.5577 18.2012 14.732 13.7989 11.1331 10.7287 18.9944
Translational Efficiency 1.6692 1.7939 0.9238 0.7427 1.2785 1.5485 1.356 1.3627 1.2763 1.2767

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vts1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available