Standard name | |
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Human Ortholog | |
Description | Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 9 | 6 | 5 | 7 | 5 | 0 | 7 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud | 0 | 0 | 1 | 0 | 0 | 3 | 6 | 9 | 17 | 14 | 5 | 8 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 9 | 12 | 18 | 21 | 24 | 14 | 21 | 11 | 9 | 10 | 17 | 13 | 23 | 8 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 21 | 103 | 219 | 256 | 370 | 364 | 525 | 598 | 324 | 389 | 205 | 247 | 263 | 536 | 521 | 392 | 227 | 229 | 37 | 104 | 187 | 121 | 355 | 362 |
Endoplasmic Reticulum | 0 | 0 | 3 | 6 | 12 | 14 | 30 | 47 | 20 | 21 | 23 | 14 | 8 | 10 | 4 | 17 | 10 | 10 | 4 | 2 | 21 | 11 | 15 | 35 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 22 | 1 | 3 | 3 | 0 | 0 | 7 | 6 | 2 | 4 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 0 | 1 | 0 | 2 | 0 |
Mitochondria | 0 | 3 | 1 | 6 | 26 | 121 | 202 | 223 | 256 | 303 | 164 | 208 | 0 | 1 | 2 | 16 | 5 | 4 | 0 | 0 | 4 | 1 | 3 | 6 |
Nucleus | 0 | 0 | 1 | 2 | 0 | 2 | 4 | 4 | 1 | 4 | 0 | 1 | 1 | 0 | 1 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 9 | 9 | 24 | 55 | 18 | 20 | 12 | 10 | 2 | 4 | 0 | 7 | 4 | 7 | 0 | 1 | 5 | 2 | 3 | 9 |
Unique Cell Count | 21 | 104 | 224 | 267 | 407 | 442 | 724 | 875 | 534 | 621 | 358 | 406 | 272 | 557 | 529 | 418 | 238 | 240 | 44 | 110 | 230 | 143 | 394 | 436 |
Labelled Cell Count | 21 | 107 | 229 | 280 | 432 | 533 | 821 | 993 | 658 | 782 | 432 | 503 | 284 | 584 | 550 | 464 | 267 | 260 | 44 | 110 | 230 | 143 | 394 | 436 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.8 | 9.5 | 9.8 | 9.5 | 8.7 | 7.0 | 6.6 | 6.7 | 6.2 | 6.0 | 6.1 | 5.7 | 10.5 | 9.8 | 9.5 | 10.6 | 12.6 | 13.6 | 10.7 | 10.2 | 10.6 |
Std Deviation (1e-4) | 1.1 | 1.4 | 1.4 | 1.7 | 1.8 | 1.4 | 1.4 | 1.4 | 1.4 | 1.2 | 1.2 | 1.1 | 1.5 | 1.1 | 1.4 | 2.2 | 2.7 | 3.2 | 1.7 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.17 | -0.49 | -0.57 | -0.55 | -0.68 | -0.72 | -0.69 | -0.79 | 0.1 | -0.0 | -0.05 | 0.11 | 0.36 | 0.47 | 0.12 | 0.05 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.8 | 0 | 2.3 | 1.7 | 1.6 | 0 | 2.0 | 1.7 | 0 | 2.0 | 1.8 | 0 | 2.9 | 0 | 0 |
Cytoplasm | -1.2 | -3.3 | -5.7 | -8.0 | -9.0 | -10.3 | -10.0 | -10.7 | -10.1 | -0.7 | -1.1 | 0.7 | -2.2 | -1.4 | -1.4 |
Endoplasmic Reticulum | 0 | 1.3 | 1.4 | 2.0 | 2.6 | 1.8 | 1.6 | 2.9 | 1.6 | 0 | 0 | 0 | 1.9 | 1.9 | 1.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 3.5 | 8.5 | 8.8 | 8.3 | 12.6 | 12.9 | 11.8 | 13.0 | 0 | 0 | 0 | 2.5 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.3 | 3.5 | 2.3 | 2.3 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.6396 | 5.0089 | 4.3239 | 4.051 | 3.3026 | 4.2319 | 3.0319 | 5.4401 | 4.6616 | 4.3687 | 3.9514 | 4.5962 | 6.1744 | 8.0923 | 6.9677 | 6.6574 | 6.02 | 6.6289 |
Actin | 0.1218 | 0.0027 | 0.0019 | 0.0011 | 0.0012 | 0.0018 | 0.0453 | 0.0012 | 0.0125 | 0.0006 | 0.0033 | 0.0009 | 0.0051 | 0.0008 | 0.0141 | 0.001 | 0.0004 | 0.0018 |
Bud | 0.0021 | 0.0006 | 0.0008 | 0.0005 | 0.0005 | 0.0007 | 0.0005 | 0.0003 | 0.0007 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0006 | 0.0006 | 0.0001 | 0.0002 |
Bud Neck | 0.0034 | 0.0005 | 0.0006 | 0.0007 | 0.0024 | 0.0013 | 0.0011 | 0.0001 | 0.0012 | 0.0004 | 0.0003 | 0.0009 | 0.0004 | 0.0002 | 0.0005 | 0.0006 | 0.0004 | 0.0009 |
Bud Periphery | 0.0031 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 |
Bud Site | 0.0099 | 0.0036 | 0.0018 | 0.0004 | 0.0002 | 0.0002 | 0.0022 | 0.0008 | 0.0035 | 0.0003 | 0.0002 | 0.0002 | 0.0009 | 0.002 | 0.0028 | 0.0004 | 0.0001 | 0.0001 |
Cell Periphery | 0.0011 | 0.0012 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0 | 0.0001 |
Cytoplasm | 0.5642 | 0.9297 | 0.8438 | 0.8493 | 0.8833 | 0.8061 | 0.6391 | 0.9434 | 0.8389 | 0.8305 | 0.7596 | 0.8708 | 0.8345 | 0.9723 | 0.9039 | 0.8614 | 0.9243 | 0.9051 |
Cytoplasmic Foci | 0.0373 | 0.0074 | 0.0134 | 0.0207 | 0.0216 | 0.0205 | 0.0535 | 0.0131 | 0.0253 | 0.0629 | 0.0332 | 0.0212 | 0.0173 | 0.0079 | 0.0148 | 0.0262 | 0.0172 | 0.0185 |
Eisosomes | 0.0013 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0796 | 0.0256 | 0.0419 | 0.0157 | 0.017 | 0.0555 | 0.0232 | 0.0048 | 0.0077 | 0.0049 | 0.0045 | 0.0231 | 0.0511 | 0.0035 | 0.016 | 0.0162 | 0.0108 | 0.02 |
Endosome | 0.0637 | 0.0115 | 0.0475 | 0.0877 | 0.0569 | 0.0702 | 0.1009 | 0.026 | 0.0666 | 0.0756 | 0.0659 | 0.0495 | 0.0443 | 0.0078 | 0.0294 | 0.0749 | 0.0371 | 0.0342 |
Golgi | 0.0208 | 0.0024 | 0.0044 | 0.0066 | 0.0029 | 0.0159 | 0.0366 | 0.002 | 0.0161 | 0.0059 | 0.026 | 0.0073 | 0.0115 | 0.0017 | 0.0081 | 0.0043 | 0.0027 | 0.0064 |
Lipid Particles | 0.0193 | 0.0002 | 0.0011 | 0.0002 | 0.0001 | 0.0015 | 0.0237 | 0.0001 | 0.008 | 0.0002 | 0.0164 | 0.0004 | 0.0016 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0027 |
Mitochondria | 0.0193 | 0.0006 | 0.0215 | 0.0015 | 0.0008 | 0.0111 | 0.0124 | 0.0004 | 0.0066 | 0.0006 | 0.0031 | 0.012 | 0.003 | 0.0002 | 0.0014 | 0.0011 | 0.0015 | 0.0019 |
None | 0.0088 | 0.0019 | 0.0014 | 0.0027 | 0.0006 | 0.001 | 0.0328 | 0.002 | 0.0026 | 0.0022 | 0.0353 | 0.0018 | 0.012 | 0.0008 | 0.0012 | 0.0012 | 0.0012 | 0.0009 |
Nuclear Periphery | 0.0045 | 0.0017 | 0.0027 | 0.0017 | 0.0018 | 0.0027 | 0.0043 | 0.0004 | 0.0011 | 0.0012 | 0.0272 | 0.0008 | 0.0057 | 0.0002 | 0.001 | 0.0018 | 0.0008 | 0.0007 |
Nucleolus | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0.0003 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0018 | 0.0014 | 0.0013 | 0.001 | 0.0015 | 0.0012 | 0.0015 | 0.0004 | 0.0007 | 0.001 | 0.0099 | 0.0047 | 0.0019 | 0.0003 | 0.0007 | 0.0011 | 0.0009 | 0.0007 |
Peroxisomes | 0.0221 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.008 | 0 | 0.0021 | 0.0038 | 0.004 | 0.0005 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0022 |
Punctate Nuclear | 0.0026 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0028 | 0.0001 | 0.0003 | 0.0003 | 0.0051 | 0.0001 | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0007 |
Vacuole | 0.0097 | 0.0074 | 0.0126 | 0.007 | 0.006 | 0.006 | 0.0076 | 0.0038 | 0.0039 | 0.007 | 0.0033 | 0.0032 | 0.007 | 0.001 | 0.0027 | 0.0062 | 0.0016 | 0.0019 |
Vacuole Periphery | 0.003 | 0.0011 | 0.0022 | 0.0024 | 0.0025 | 0.0027 | 0.002 | 0.0006 | 0.0015 | 0.002 | 0.0018 | 0.0015 | 0.0015 | 0.0002 | 0.0007 | 0.002 | 0.0007 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.1968 | 12.5493 | 25.6084 | 20.5577 | 18.2012 | 14.732 | 13.7989 | 11.1331 | 10.7287 | 18.9944 |
Translational Efficiency | 1.6692 | 1.7939 | 0.9238 | 0.7427 | 1.2785 | 1.5485 | 1.356 | 1.3627 | 1.2763 | 1.2767 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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