Standard name
Human Ortholog
Description Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.99 0.98 0.97 0.9 0.86 0.77 0.68 – 0.69 0.63 0.98 0.99 0.98 0.92 0.94 0.96 0.95 0.88 0.88 0.88 0.88 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0.13 0.11 0.29 0.34 0.39 0.54 – 0.56 0.61 0 0 0.06 0.07 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 1 1 – 0 0 0 0 0 0 0 0 1 0 1 0 0 1
Bud 1 1 5 3 1 6 7 15 6 – 15 8 0 2 4 0 0 0 0 0 0 0 2 4
Bud Neck 0 0 0 0 0 0 1 1 0 – 0 0 0 0 0 2 1 0 1 0 3 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 9 2 3 0 5 8 8 13 3 – 8 12 0 5 3 7 15 9 0 0 0 0 0 0
Cytoplasm 380 115 273 212 300 374 541 492 248 – 292 345 146 248 281 390 329 364 376 126 245 95 138 161
Endoplasmic Reticulum 1 0 0 0 0 3 1 2 7 – 6 8 1 0 1 21 14 26 1 1 2 1 0 2
Endosome 0 0 0 0 1 0 5 0 0 – 0 1 1 0 0 8 4 5 1 2 1 2 1 2
Golgi 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 2 0 2 0 0 0 0 1 0
Mitochondria 0 10 7 29 34 123 214 250 198 – 238 334 1 3 18 29 5 6 4 4 12 2 3 5
Nucleus 2 0 0 1 0 0 1 7 2 – 2 2 1 0 2 1 3 2 0 0 1 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 1 0 – 0 0 0 0 0 1 1 1 1 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 – 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 – 1 0 0 0 0 4 0 0 0 0 0 0 0 3
Vac/Vac Membrane 3 1 0 0 2 1 9 9 2 – 3 12 0 1 0 27 22 14 4 2 4 1 4 4
Unique Cell Count 385 120 276 217 308 417 630 640 366 425 550 149 251 286 426 351 381 396 144 279 108 158 192
Labelled Cell Count 397 130 288 246 343 515 787 794 467 566 723 150 259 309 493 394 430 396 144 279 108 158 192


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.5 4.0 4.0 4.4 4.2 4.0 4.2 3.9 – 3.8 3.6 5.3 5.4 4.7 5.5 5.9 5.9 5.2 5.6 5.9
Std Deviation (1e-4) 0.7 0.8 0.8 1.2 1.2 1.1 0.9 1.1 0.9 – 1.0 0.8 1.3 1.4 0.9 1.3 1.6 1.8 1.4 2.2 1.7
Intensity Change (Log2) – – – -0.03 0.13 0.06 -0.01 0.04 -0.06 – -0.11 -0.18 0.4 0.43 0.22 0.44 0.55 0.55 0.36 0.48 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.3 4.2 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5536 3.4691 2.9511 2.7269 2.6604 2.7398 2.3785 2.0252 1.7648 1.5773 1.7141 1.6948 0.314 2.6341 1.5686 2.0263 1.1511 1.8077
Actin 0.0003 0.0004 0.0044 0.0008 0.0002 0.0009 0.0413 0.0064 0.0172 0.0228 0.0104 0.0104 0.0001 0.0001 0.0009 0.0002 0.0004 0.0058
Bud 0.0002 0.0007 0.0005 0.0002 0.0004 0.0001 0.005 0.005 0.0026 0.002 0.0005 0.0015 0 0.0001 0.0044 0.0001 0.0001 0.0001
Bud Neck 0.0002 0.0004 0.0015 0.0005 0.0002 0.0013 0.0041 0.0002 0.0007 0.006 0.0004 0.0035 0 0 0.0002 0.0001 0.0012 0.0005
Bud Periphery 0.0004 0.0007 0.0015 0.0004 0.0007 0.0002 0.0117 0.0101 0.0065 0.003 0.0004 0.0055 0 0.0001 0.0064 0.0001 0.0001 0.0001
Bud Site 0.0005 0.0125 0.0013 0.0003 0.0001 0.0001 0.0138 0.0088 0.0113 0.0128 0.0005 0.0002 0.0001 0.0004 0.0006 0.0003 0.0001 0.0002
Cell Periphery 0.0009 0.0016 0.0019 0.0011 0.0002 0.0004 0.0018 0.0005 0.0003 0.0003 0.0001 0.0003 0.0001 0.0004 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.6808 0.8151 0.7423 0.7513 0.6557 0.8504 0.3215 0.6501 0.55 0.6036 0.5791 0.6947 0.2784 0.3357 0.3687 0.2841 0.3916 0.3981
Cytoplasmic Foci 0.0082 0.0061 0.0111 0.0098 0.004 0.0091 0.0547 0.018 0.028 0.0316 0.0149 0.0169 0.0042 0.0046 0.0095 0.0049 0.0091 0.0157
Eisosomes 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0001 0.0002 0.001 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0003 0.0001
Endoplasmic Reticulum 0.0108 0.0042 0.0037 0.022 0.0025 0.006 0.0168 0.0026 0.0024 0.0028 0.0016 0.0041 0.0014 0.0022 0.0012 0.0008 0.002 0.0015
Endosome 0.01 0.0061 0.005 0.0332 0.0027 0.0124 0.0408 0.0057 0.0116 0.0415 0.0431 0.0092 0.0004 0.0016 0.0163 0.0035 0.0052 0.0034
Golgi 0.0006 0.0006 0.0008 0.002 0.0002 0.0011 0.011 0.0015 0.0031 0.0149 0.0511 0.003 0.0001 0.0002 0.0023 0.0001 0.0002 0.001
Lipid Particles 0.0008 0.0004 0.0003 0.0011 0.0001 0.0003 0.012 0.0019 0.0018 0.0021 0.0177 0.0009 0.0001 0.0005 0.0043 0.0002 0.0002 0.0018
Mitochondria 0.0004 0.0023 0.0142 0.0023 0.0001 0.0008 0.0159 0.0011 0.0049 0.0185 0.0222 0.002 0.0001 0.0002 0.0046 0.0001 0.0007 0.0003
None 0.279 0.1402 0.2063 0.1572 0.3299 0.1131 0.3653 0.2803 0.3477 0.2233 0.2424 0.2369 0.7139 0.6505 0.5665 0.7029 0.5149 0.5668
Nuclear Periphery 0.0009 0.0008 0.0004 0.003 0.0002 0.0004 0.0199 0.0006 0.0018 0.0007 0.0018 0.0011 0.0002 0.0004 0.0003 0.0003 0.0091 0.0004
Nucleolus 0.0001 0.0001 0.0001 0.0001 0 0 0.0092 0.0001 0.0002 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0048 0.0001
Nucleus 0.0009 0.0014 0.0008 0.0015 0.0003 0.0007 0.008 0.0008 0.001 0.001 0.0007 0.001 0.0002 0.0005 0.0006 0.0004 0.0534 0.0003
Peroxisomes 0.0001 0.0001 0.0003 0.0001 0 0.0001 0.0137 0.003 0.0038 0.0039 0.0088 0.0064 0 0.0001 0.0096 0.0001 0.0001 0.0009
Punctate Nuclear 0.0003 0.0003 0.0002 0.0005 0.0001 0.0001 0.0197 0.0009 0.0018 0.0006 0.0004 0.0007 0.0001 0.0002 0.0003 0.0003 0.0044 0.0021
Vacuole 0.0042 0.0053 0.0028 0.0098 0.0023 0.0022 0.0102 0.0021 0.0024 0.0051 0.002 0.0011 0.0003 0.0018 0.0024 0.0009 0.0017 0.0005
Vacuole Periphery 0.0003 0.0005 0.0005 0.0026 0.0003 0.0003 0.0029 0.0003 0.0007 0.002 0.0015 0.0003 0 0.0001 0.0004 0.0002 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.0714 13.9683 8.9698 14.8616 10.9088 13.5146 13.8517 16.1316 16.9846 21.615
Translational Efficiency 0.9392 0.7179 1.0503 0.6815 0.8631 1.0758 0.9235 0.7669 0.7322 0.7337

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gde1

Gde1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gde1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available