Standard name
Human Ortholog
Description ATP binding cassette protein; cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.07 0.05 0.08 0.13 0.09 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 1.0 0.99 0.95 0.95 0.8 0.79 0.75 0.69 0.67 0.68 0.99 0.99 0.98 0.99 0.99 0.99 0.97 0.95 0.93 0.92 0.95 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0.15 0.14 0.12 0.17 0.15 0.12 0 0 0 0 0.06 0 0 0 0 0 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.15 0.17 0.2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 4 23 16 17 18 12 24 0 0 2 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 0 3 3 3 1 0 0 0 0 0 1 3 0 2 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 13 2 0 3 12 19 39 28 24 46 30 50 0 1 4 1 4 7 1 0 0 0 0 0
Cytoplasm 548 46 207 240 380 383 452 419 214 240 215 238 146 234 259 260 265 347 526 62 198 116 245 208
Endoplasmic Reticulum 5 0 0 2 17 9 83 72 34 59 48 41 6 3 3 7 17 13 5 0 4 4 6 14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 1 2 0 0 0 0 1 0 0
Mitochondria 0 0 0 0 4 6 15 19 34 51 54 70 0 0 2 1 0 1 0 0 1 0 0 0
Nucleus 0 0 0 1 0 1 1 3 1 2 1 2 0 1 0 0 0 0 0 0 1 1 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 6 7 1 1 3 5 0 0 0 2 2 3 1 0 2 1 1 0
Unique Cell Count 552 46 207 242 401 402 568 528 285 350 319 351 148 237 264 263 268 352 541 66 213 126 258 233
Labelled Cell Count 566 48 207 246 416 422 620 565 328 421 367 433 152 239 270 272 290 372 541 66 213 126 258 233


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 59.8 48.9 31.8 31.6 26.6 22.3 19.8 18.6 16.2 15.4 14.2 13.5 49.3 47.4 40.1 50.2 62.0 65.7 51.6 52.8 53.5
Std Deviation (1e-4) 12.5 7.8 7.4 7.9 5.4 4.2 3.9 3.6 3.2 3.1 3.1 2.9 11.6 10.2 9.2 19.2 21.1 19.7 14.0 11.7 15.7
Intensity Change (Log2) – – – -0.01 -0.26 -0.51 -0.68 -0.77 -0.97 -1.05 -1.16 -1.23 0.63 0.58 0.34 0.66 0.96 1.05 0.7 0.73 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.9 0 3.6 3.3 0 3.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 3.2 3.9 3.4 4.3 5.4 4.5 5.7 0 0 0 0 0 0
Cytoplasm -1.3 -3.4 -3.2 -7.1 -7.1 -7.8 -9.0 -9.2 -9.1 -1.7 -1.6 -2.0 -1.5 -1.5 -1.7
Endoplasmic Reticulum 0 3.0 0 5.8 5.6 5.2 6.2 5.9 5.1 0 0 0 0 3.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2.8 5.2 5.8 6.2 6.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 45.7716 58.8505 52.7011 51.4057 50.3663 56.8541 43.6632 62.3244 55.4563 52.2335 52.3719 58.6429 35.0818 54.1758 45.7768 48.8726 40.5035 44.6703
Actin 0.0018 0.0016 0.0021 0.0023 0.0009 0.001 0.0152 0.0005 0.0104 0.0008 0.0037 0.0004 0.0183 0.0004 0.0018 0.0002 0.036 0.0026
Bud 0.0013 0.0014 0.0018 0.0022 0.0014 0.0016 0.001 0.0006 0.001 0.0011 0.0007 0.0003 0.0019 0.0004 0.0007 0.001 0.0003 0.0011
Bud Neck 0.0015 0.0022 0.0013 0.0056 0.0013 0.0026 0.0019 0.0013 0.0012 0.0008 0.0009 0.0013 0.001 0.0003 0.0014 0.0004 0.0008 0.0012
Bud Periphery 0.0016 0.0013 0.0016 0.0021 0.0004 0.0009 0.0015 0.0003 0.0013 0.0004 0.0006 0.0002 0.0019 0.0004 0.0002 0.0002 0.0002 0.0005
Bud Site 0.0006 0.002 0.0012 0.0009 0.0002 0.0004 0.0032 0.0005 0.0024 0.0006 0.0003 0.0001 0.0022 0.0004 0.0012 0.0001 0.0005 0.0002
Cell Periphery 0.0119 0.016 0.0081 0.0225 0.0003 0.0027 0.0074 0.0034 0.0037 0.0015 0.0045 0.0002 0.0019 0.0025 0.0007 0.0005 0.0001 0.0005
Cytoplasm 0.5737 0.8421 0.7537 0.5328 0.9242 0.8459 0.795 0.9612 0.9007 0.8842 0.8633 0.9156 0.7247 0.9596 0.9128 0.8211 0.8249 0.9093
Cytoplasmic Foci 0.0015 0.001 0.0017 0.0017 0.0053 0.004 0.0062 0.0009 0.0021 0.0084 0.0036 0.002 0.0121 0.0011 0.0159 0.0035 0.0125 0.0065
Eisosomes 0 0 0.0001 0 0 0 0.0003 0.0001 0.0003 0 0.0009 0 0.0002 0 0 0 0.0002 0
Endoplasmic Reticulum 0.172 0.0465 0.0547 0.1098 0.0026 0.0891 0.0458 0.0062 0.0156 0.0029 0.0453 0.0331 0.0582 0.0182 0.0051 0.0217 0.0013 0.0344
Endosome 0.0073 0.0032 0.0066 0.0104 0.0166 0.0136 0.0159 0.0034 0.0068 0.0161 0.0057 0.0024 0.0365 0.0012 0.0174 0.0126 0.0213 0.0193
Golgi 0.0008 0.0008 0.0006 0.0009 0.0006 0.0017 0.0032 0.0003 0.001 0.0008 0.0015 0.0002 0.0097 0.0003 0.0068 0.0004 0.0251 0.0028
Lipid Particles 0.0009 0.0006 0.0005 0.0012 0.0001 0.0003 0.0045 0.0003 0.0008 0.0012 0.0011 0.0002 0.0145 0.0001 0.0072 0.0001 0.0124 0.0001
Mitochondria 0.0021 0.0009 0.0108 0.0023 0.0006 0.0061 0.0169 0.0015 0.0055 0.0006 0.0052 0.0028 0.025 0.0001 0.0014 0.0002 0.0431 0.0014
None 0.0006 0.0002 0.0003 0.0003 0.0003 0.0003 0.0022 0.0001 0.0004 0.0009 0.0011 0.0012 0.0061 0.0001 0.0005 0.0002 0.0002 0.0002
Nuclear Periphery 0.0456 0.0161 0.0156 0.028 0.0086 0.0059 0.0188 0.0028 0.0058 0.0036 0.0087 0.0073 0.0235 0.0013 0.0021 0.0038 0.0008 0.0022
Nucleolus 0.0002 0.0001 0.0004 0.0006 0.0001 0.0001 0.0004 0.0001 0.0002 0.0003 0.0001 0.0038 0.0012 0 0.0002 0 0 0
Nucleus 0.0112 0.0043 0.0052 0.0101 0.0085 0.0032 0.0076 0.0022 0.0033 0.0042 0.0021 0.0213 0.0077 0.0008 0.0012 0.0016 0.0007 0.0011
Peroxisomes 0 0 0.0002 0.0001 0.0001 0.0001 0.001 0 0.0003 0.0001 0.0001 0.0001 0.0039 0 0.0014 0 0.0047 0.0001
Punctate Nuclear 0.0002 0.0001 0 0.0001 0.0003 0.0001 0.0017 0.0001 0.0003 0.0004 0.0001 0.0009 0.0027 0 0.0003 0 0.0002 0
Vacuole 0.1297 0.0519 0.1122 0.2041 0.0214 0.0141 0.0449 0.0125 0.0317 0.062 0.0462 0.0046 0.0364 0.0121 0.0184 0.1064 0.0119 0.0128
Vacuole Periphery 0.0355 0.0077 0.0214 0.062 0.0061 0.0061 0.0054 0.0016 0.0049 0.0092 0.0041 0.0021 0.0105 0.0007 0.0032 0.0256 0.0029 0.0037

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 168.8445 177.9927 172.6393 160.4704 141.2286 143.7225 143.7246 140.5861 126.6622 147.3015
Translational Efficiency 1.1278 1.1387 1.0411 0.9892 1.1013 1.0848 1.0403 0.8411 1.0119 1.045

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ATP binding cassette protein; cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

New1

New1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (New1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available