Standard name
Human Ortholog
Description Putative ortholog of mammalian ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response; ETF-alpha is an electron transfer flavoprotein complex subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0.07 0 0 0 0 0 0 0 0 0 0 0 0.18 – 0.22 0 0.05 0 0 0 0.06
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0 0 0 0 0 0
Cytoplasm 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Endosome 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 – 0.25 0.24 0 0 0 0 0 0 0 0.19 0.24 0.49 0.48 – 0.49 0 0.13 0.05 0.11 0.11 0.13
Mitochondria 0.97 0.88 – 0.89 0.84 0.84 0.86 0.83 0.88 0.9 0.9 0.89 0.94 0.92 0.78 0.15 – 0.09 0.89 0.71 0.76 0.76 0.73 0.69
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0.05 0 0.07 0 0.05 0 0 0 0 0 – 0.07 0 0 0 0 0 0
Nucleolus 0.18 0.06 – 0.06 0.07 0.12 0.17 0.15 0.14 0.13 0.12 0.13 0.06 0 0 0.13 – 0.07 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.12 – 0.05 0.07 0.09 0.06 0.05 0 0.06 0 0.06 0 0.06 0.09 0.14 – 0.2 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 14 5 2 3 2 1 1 1 4 8 6 7 26 – 10 4 3 1 3 10 22
Bud 1 0 – 2 2 4 5 5 4 4 3 8 1 0 2 4 – 0 0 0 0 0 2 1
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 2 2 1 1 1 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 – 7 3 1 1 1 2 1 0 0 7 6 4 5 – 3 0 0 0 0 0 0
Cytoplasm 0 0 – 5 6 7 7 9 12 8 3 5 0 0 6 2 – 2 0 0 0 0 1 1
Endoplasmic Reticulum 0 0 – 1 0 2 1 0 1 1 1 1 2 0 1 5 – 2 0 0 0 0 0 0
Endosome 0 1 – 1 0 0 0 2 0 1 0 1 0 0 1 2 – 0 0 0 0 0 3 4
Golgi 3 0 – 50 62 8 10 12 0 6 0 11 40 64 136 69 – 22 6 7 2 11 34 50
Mitochondria 185 15 – 176 220 246 380 314 227 257 189 306 193 248 216 21 – 4 168 40 33 81 228 266
Nucleus 0 0 – 1 2 4 0 2 0 0 1 3 0 1 0 3 – 0 0 0 0 0 1 0
Nuclear Periphery 7 0 – 6 7 7 21 15 17 12 10 7 8 5 8 2 – 3 0 0 0 1 3 3
Nucleolus 34 1 – 11 18 36 76 56 35 38 25 43 12 11 3 19 – 3 1 0 1 1 5 4
Peroxisomes 1 0 – 2 3 1 4 3 1 1 0 1 0 0 1 0 – 0 4 0 1 4 14 17
SpindlePole 0 0 – 0 0 1 0 4 1 2 0 0 0 0 0 0 – 0 0 0 1 0 0 1
Vac/Vac Membrane 17 2 – 9 18 26 25 19 11 17 8 20 8 17 26 20 – 9 1 4 1 0 5 6
Unique Cell Count 191 17 197 261 292 442 378 257 286 209 343 206 270 276 143 45 190 57 44 107 314 386
Labelled Cell Count 248 19 285 346 345 533 446 314 350 242 411 279 358 411 178 58 190 57 44 107 314 386


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.7 26.6 – 24.9 25.6 23.3 21.6 21.1 19.6 19.1 16.7 17.2 29.4 31.0 29.5 32.5 – 30.9 23.4 26.9 29.6
Std Deviation (1e-4) 6.3 10.5 – 6.0 5.9 6.0 5.5 6.1 6.5 6.3 4.9 4.9 6.3 6.4 6.6 8.8 – 8.1 7.0 8.4 8.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 28.0639 33.2786 30.7356 26.2622 29.1639 32.0438 31.7536 32.8663 30.591 22.5526 21.7183 31.4444 29.2787 31.745 26.7358 29.6638 29.6785 28.9104
Actin 0.0131 0.0035 0.0051 0.0273 0.0217 0.0004 0.0125 0.0005 0.0085 0.0012 0.0033 0.0322 0.0014 0.0015 0.0024 0.0561 0.0004 0.002
Bud 0.0003 0.0001 0.0001 0.001 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0001 0.0009 0 0.0001
Bud Neck 0.0002 0.0002 0.0008 0.0009 0.0003 0 0.0004 0 0.0001 0 0.0003 0.0001 0.0005 0 0.0001 0.0006 0 0
Bud Periphery 0.0004 0.0001 0.0002 0.0019 0.0003 0 0.0002 0.0002 0.0002 0.0001 0.0003 0.0001 0.0006 0.0002 0.0001 0.0007 0.0001 0.0002
Bud Site 0.0011 0.0008 0.0003 0.0027 0.0008 0 0.0004 0.0001 0.0007 0.0004 0.0031 0.0001 0.0015 0.0002 0.0005 0.001 0 0.0001
Cell Periphery 0.0002 0.0003 0.0002 0.0003 0.0003 0 0.0008 0.0005 0.0002 0.0008 0.0003 0.0002 0.0004 0.0004 0.0001 0.0001 0.0002 0.0001
Cytoplasm 0.0005 0.0001 0.0001 0.0005 0.0018 0 0.0018 0 0.0018 0.0008 0.0018 0 0.0009 0 0 0.0417 0 0
Cytoplasmic Foci 0.0089 0.0009 0.0027 0.0071 0.014 0 0.0087 0.0001 0.001 0.0165 0.0586 0.0007 0.0019 0.0006 0.0067 0.0111 0.0001 0.0006
Eisosomes 0.0006 0.0007 0.001 0.0003 0.0002 0.0002 0.0004 0.0002 0.0006 0.0005 0.0006 0.0003 0.0003 0.0003 0.0004 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.0004 0 0.0002 0.0004 0.0002 0 0.0002 0 0.0003 0.0002 0.001 0.0001 0.0011 0 0 0.0001 0 0
Endosome 0.0064 0.0018 0.0023 0.0115 0.0079 0.0004 0.0035 0.0004 0.0022 0.0032 0.0175 0.0005 0.0084 0.0009 0.002 0.0017 0.0001 0.0009
Golgi 0.0066 0.0067 0.004 0.0056 0.0066 0.0045 0.0018 0.0009 0.0014 0.0041 0.0051 0.0017 0.0009 0.0005 0.0027 0.0015 0.0003 0.0021
Lipid Particles 0.0257 0.0081 0.0056 0.062 0.0378 0.0022 0.0167 0.0035 0.0042 0.0333 0.0485 0.0187 0.003 0.009 0.0208 0.0013 0.0011 0.0205
Mitochondria 0.9105 0.9464 0.9648 0.8322 0.8733 0.9849 0.8987 0.9597 0.9594 0.7771 0.7997 0.914 0.8974 0.9249 0.9471 0.8753 0.9692 0.9587
None 0.0006 0 0.0001 0.0006 0.0007 0 0.002 0 0.001 0.0049 0.0064 0.0001 0.0008 0 0.0002 0.0002 0 0.0001
Nuclear Periphery 0.0004 0.0001 0.0005 0.0016 0.0005 0.0005 0.0021 0.0001 0.0024 0.0009 0.0039 0.0018 0.0245 0.0004 0.0001 0 0 0.0002
Nucleolus 0.0017 0.0002 0.0004 0.0013 0.0038 0.0001 0.0058 0.0005 0.0007 0.0312 0.0141 0.0089 0.0029 0.0014 0.0003 0.0001 0.0002 0.0004
Nucleus 0.0003 0.0001 0.0001 0.0014 0.0006 0 0.0015 0.0002 0.0031 0.0029 0.0032 0.0022 0.0044 0.0002 0.0001 0.0001 0.0002 0.0001
Peroxisomes 0.0096 0.0011 0.0014 0.0043 0.0061 0.0003 0.0205 0.0007 0.0031 0.0474 0.0094 0.0013 0.0012 0.0067 0.015 0.0053 0.0104 0.0061
Punctate Nuclear 0.0005 0 0.0008 0.0006 0.0003 0 0.001 0 0.0015 0.0012 0.0078 0.0001 0.0303 0 0.0004 0.0001 0 0
Vacuole 0.0021 0.003 0.0011 0.0093 0.0055 0.0003 0.0061 0.0061 0.0022 0.0081 0.0029 0.0014 0.0115 0.0105 0.0003 0.0004 0.0022 0.0009
Vacuole Periphery 0.0099 0.0258 0.0082 0.0271 0.017 0.0062 0.0147 0.026 0.0055 0.0651 0.0119 0.0155 0.0058 0.0419 0.0007 0.0014 0.0151 0.0067

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 51.7054 49.5571 40.3094 47.1711 46.3048 56.4716 78.0514 75.7674 68.2364 62.9134
Translational Efficiency 1.0788 1.4389 1.2089 1.0165 1.0092 1.0291 1.092 1.0368 1.046 1.0227

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative ortholog of mammalian ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response; ETF-alpha is an electron transfer flavoprotein complex subunit
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Aim45

Aim45


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim45-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available