Standard name | |
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Human Ortholog | |
Description | Mitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 7 | 2 | 1 | 6 | 1 | 3 | 5 | 4 | 6 | 0 | 3 | 4 |
Bud | 1 | 2 | 3 | 8 | 16 | 24 | 28 | 22 | – | 19 | 27 | 44 | 6 | 4 | 6 | 6 | 4 | 3 | 0 | 1 | 0 | 1 | 2 | 1 |
Bud Neck | 1 | 0 | 0 | 1 | 1 | 3 | 6 | 1 | – | 4 | 1 | 2 | 4 | 4 | 9 | 4 | 3 | 1 | 1 | 2 | 1 | 2 | 2 | 5 |
Bud Site | 1 | 0 | 1 | 0 | 0 | 14 | 6 | 4 | – | 9 | 13 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 0 | 4 | 1 | 0 | 2 | 4 | – | 0 | 1 | 5 | 3 | 1 | 3 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 42 | 80 | 81 | 159 | 208 | 291 | 354 | 424 | – | 363 | 206 | 280 | 118 | 333 | 424 | 59 | 30 | 57 | 18 | 33 | 30 | 15 | 24 | 40 |
Endoplasmic Reticulum | 17 | 4 | 2 | 1 | 0 | 5 | 1 | 1 | – | 1 | 1 | 2 | 2 | 6 | 2 | 2 | 7 | 5 | 0 | 3 | 2 | 0 | 4 | 2 |
Endosome | 1 | 8 | 1 | 7 | 12 | 2 | 3 | 4 | – | 6 | 1 | 1 | 0 | 8 | 6 | 3 | 8 | 7 | 21 | 9 | 8 | 6 | 3 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 1 |
Mitochondria | 87 | 32 | 63 | 103 | 236 | 378 | 563 | 463 | – | 474 | 423 | 636 | 6 | 5 | 8 | 14 | 10 | 5 | 116 | 99 | 85 | 46 | 100 | 103 |
Nucleus | 0 | 1 | 0 | 0 | 3 | 4 | 11 | 21 | – | 6 | 5 | 7 | 1 | 2 | 0 | 2 | 1 | 5 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 1 | 2 | 3 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 3 | – | 1 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 7 | 17 | 3 | 6 | 2 | – | 3 | 3 | 4 | 0 | 1 | 11 | 1 | 1 | 0 | 4 | 0 | 4 | 0 | 0 | 0 |
SpindlePole | 8 | 2 | 3 | 11 | 21 | 21 | 44 | 55 | – | 64 | 38 | 39 | 2 | 3 | 7 | 11 | 8 | 7 | 5 | 5 | 5 | 1 | 8 | 3 |
Vac/Vac Membrane | 4 | 13 | 1 | 4 | 14 | 15 | 9 | 10 | – | 21 | 11 | 5 | 3 | 6 | 12 | 3 | 8 | 5 | 5 | 14 | 4 | 8 | 18 | 23 |
Unique Cell Count | 140 | 135 | 133 | 254 | 422 | 605 | 842 | 834 | 793 | 572 | 842 | 138 | 367 | 459 | 100 | 68 | 88 | 192 | 180 | 158 | 89 | 179 | 201 | |
Labelled Cell Count | 169 | 145 | 157 | 305 | 530 | 764 | 1036 | 1014 | 972 | 734 | 1037 | 152 | 375 | 489 | 113 | 84 | 100 | 192 | 180 | 158 | 89 | 179 | 201 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.4 | 3.4 | 3.4 | 3.4 | 3.3 | 2.6 | 3.0 | – | 2.7 | 2.6 | 2.6 | 4.8 | 4.8 | 4.7 | 4.9 | 4.9 | 7.0 | 5.4 | 5.6 | 5.3 |
Std Deviation (1e-4) | 0.5 | 1.0 | 0.9 | 1.4 | 1.4 | 1.5 | 1.1 | 1.4 | – | 1.2 | 1.0 | 0.8 | 1.0 | 1.0 | 1.0 | 1.1 | 1.3 | 19.0 | 1.5 | 1.9 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.01 | -0.05 | -0.38 | -0.18 | – | -0.33 | -0.37 | -0.41 | 0.49 | 0.51 | 0.47 | 0.54 | 0.54 | 1.03 | 0.67 | 0.72 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.4865 | 0.4721 | 0.5589 | 0.2583 | 0.247 | 0.3874 | 0.9136 | 1.098 | 0.9129 | 0.9856 | 0.6091 | 1.0879 | -1.4283 | -0.9168 | -1.3489 | -0.6622 | -1.4505 | -1.0851 |
Actin | 0.014 | 0.0092 | 0.0094 | 0.0098 | 0.0018 | 0.008 | 0.0153 | 0.0206 | 0.0096 | 0.008 | 0.0027 | 0.0098 | 0.005 | 0.0235 | 0.0095 | 0.0139 | 0.0318 | 0.0128 |
Bud | 0.0009 | 0.0037 | 0.0042 | 0.0077 | 0.0014 | 0.0018 | 0.0009 | 0.0035 | 0.0025 | 0.0019 | 0.0013 | 0.0009 | 0.0006 | 0.0013 | 0.0037 | 0.0034 | 0.0016 | 0.0011 |
Bud Neck | 0.0109 | 0.0022 | 0.0096 | 0.0009 | 0.0006 | 0.0041 | 0.0068 | 0.0021 | 0.0036 | 0.0198 | 0.0008 | 0.0246 | 0.0073 | 0.0008 | 0.0076 | 0.0141 | 0.0016 | 0.0184 |
Bud Periphery | 0.0019 | 0.0054 | 0.0049 | 0.0378 | 0.0026 | 0.0043 | 0.002 | 0.0089 | 0.0058 | 0.0146 | 0.0036 | 0.0033 | 0.0014 | 0.0024 | 0.0089 | 0.0069 | 0.0044 | 0.0035 |
Bud Site | 0.0144 | 0.0393 | 0.0156 | 0.0038 | 0.0013 | 0.0033 | 0.0185 | 0.0305 | 0.0119 | 0.0132 | 0.0027 | 0.0012 | 0.0123 | 0.0265 | 0.0149 | 0.0117 | 0.0017 | 0.0015 |
Cell Periphery | 0.0012 | 0.0014 | 0.0008 | 0.0005 | 0.0005 | 0.0004 | 0.0021 | 0.0014 | 0.0028 | 0.0009 | 0.0009 | 0.0011 | 0.0016 | 0.0047 | 0.0012 | 0.0013 | 0.0003 | 0.001 |
Cytoplasm | 0.0153 | 0.0567 | 0.0299 | 0.0224 | 0.0367 | 0.0209 | 0.0221 | 0.0276 | 0.0299 | 0.0125 | 0.015 | 0.0226 | 0.0227 | 0.0364 | 0.0262 | 0.0384 | 0.0338 | 0.0372 |
Cytoplasmic Foci | 0.105 | 0.1321 | 0.1274 | 0.1282 | 0.1151 | 0.1302 | 0.1383 | 0.1109 | 0.1128 | 0.1378 | 0.0813 | 0.0921 | 0.093 | 0.1213 | 0.1042 | 0.1105 | 0.1412 | 0.081 |
Eisosomes | 0.002 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0004 | 0.0011 | 0.0014 | 0.0012 | 0.0006 | 0.0005 | 0.0005 | 0.0015 | 0.0016 | 0.0008 | 0.001 | 0.0005 | 0.0004 |
Endoplasmic Reticulum | 0.0022 | 0.0018 | 0.0022 | 0.0016 | 0.0029 | 0.0011 | 0.0022 | 0.0011 | 0.0019 | 0.001 | 0.0027 | 0.0011 | 0.0083 | 0.0017 | 0.0029 | 0.0036 | 0.002 | 0.0024 |
Endosome | 0.1578 | 0.1315 | 0.1477 | 0.1638 | 0.1496 | 0.1144 | 0.1187 | 0.1066 | 0.1086 | 0.1208 | 0.1279 | 0.0987 | 0.1723 | 0.1384 | 0.1268 | 0.0564 | 0.1545 | 0.1368 |
Golgi | 0.0628 | 0.0578 | 0.0528 | 0.0447 | 0.069 | 0.0571 | 0.0631 | 0.0647 | 0.0558 | 0.0437 | 0.067 | 0.0626 | 0.0813 | 0.0917 | 0.0589 | 0.0275 | 0.1221 | 0.0641 |
Lipid Particles | 0.101 | 0.0267 | 0.0375 | 0.042 | 0.0135 | 0.0332 | 0.0705 | 0.0159 | 0.0267 | 0.019 | 0.0176 | 0.0362 | 0.082 | 0.0314 | 0.0409 | 0.0257 | 0.077 | 0.052 |
Mitochondria | 0.2913 | 0.3153 | 0.3552 | 0.3793 | 0.4905 | 0.4133 | 0.3154 | 0.4241 | 0.413 | 0.4659 | 0.433 | 0.441 | 0.3274 | 0.359 | 0.4361 | 0.499 | 0.2708 | 0.4524 |
None | 0.0819 | 0.0757 | 0.0684 | 0.0421 | 0.0249 | 0.0576 | 0.0972 | 0.0665 | 0.0967 | 0.0235 | 0.1153 | 0.0751 | 0.0755 | 0.069 | 0.0419 | 0.079 | 0.0093 | 0.0278 |
Nuclear Periphery | 0.0032 | 0.001 | 0.0049 | 0.0008 | 0.0014 | 0.0017 | 0.001 | 0.0004 | 0.001 | 0.0004 | 0.0009 | 0.0003 | 0.0088 | 0.0008 | 0.0024 | 0.0009 | 0.0029 | 0.0061 |
Nucleolus | 0.0039 | 0.002 | 0.0025 | 0.0007 | 0.0005 | 0.001 | 0.0019 | 0.0012 | 0.0011 | 0.001 | 0.0007 | 0.001 | 0.002 | 0.0007 | 0.0017 | 0.0034 | 0.0007 | 0.0016 |
Nucleus | 0.003 | 0.0018 | 0.0022 | 0.0008 | 0.0009 | 0.0017 | 0.0012 | 0.0011 | 0.0013 | 0.0009 | 0.0009 | 0.0007 | 0.0031 | 0.0011 | 0.0015 | 0.0017 | 0.0006 | 0.0013 |
Peroxisomes | 0.1008 | 0.1188 | 0.1052 | 0.0956 | 0.0715 | 0.131 | 0.1087 | 0.0999 | 0.0948 | 0.1001 | 0.1123 | 0.1154 | 0.0648 | 0.0685 | 0.0942 | 0.047 | 0.1316 | 0.0802 |
Punctate Nuclear | 0.0071 | 0.0014 | 0.0028 | 0.0011 | 0.0021 | 0.005 | 0.0034 | 0.0011 | 0.001 | 0.0027 | 0.0009 | 0.0008 | 0.0042 | 0.0007 | 0.003 | 0.0395 | 0.0041 | 0.0012 |
Vacuole | 0.0154 | 0.0112 | 0.0123 | 0.0097 | 0.0078 | 0.006 | 0.0068 | 0.0076 | 0.0132 | 0.0079 | 0.0079 | 0.0059 | 0.0186 | 0.0137 | 0.0091 | 0.0101 | 0.0044 | 0.0108 |
Vacuole Periphery | 0.0038 | 0.0043 | 0.0041 | 0.0066 | 0.0052 | 0.0037 | 0.0028 | 0.0028 | 0.0046 | 0.0039 | 0.0043 | 0.0051 | 0.0062 | 0.005 | 0.0036 | 0.0048 | 0.003 | 0.0064 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.9349 | 14.0767 | 11.1916 | 14.4503 | 13.854 | 12.3657 | 14.233 | 13.7233 | 20.0576 | 17.4612 |
Translational Efficiency | 0.621 | 0.5791 | 0.6787 | 0.6123 | 0.5019 | 0.6798 | 0.6662 | 0.8453 | 0.4909 | 0.4573 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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