ORF | |
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Human Ortholog | |
Description | Putative membrane protein; YPR071W is not an essential gene; YPR071W has a paralog, YIL029C, that arose from a single-locus duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 9 | 32 | 10 | 7 | 4 | 1 | – | 2 | 0 | 1 | 2 | 11 | 16 | 10 | 3 | 11 | 7 | 92 | 35 | 84 | 40 | 111 | 114 |
Bud | 7 | 3 | 6 | 17 | 19 | 17 | 14 | – | 17 | 23 | 7 | 18 | 2 | 10 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 4 | 1 | 2 | 0 | 1 | – | 3 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 6 | 1 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 0 | 6 | 4 | 0 | 2 | – | 3 | 2 | 0 | 1 | 3 | 3 | 11 | 2 | 2 | 1 | 0 | 2 | 7 | 2 | 8 | 11 |
Cytoplasm | 48 | 24 | 84 | 120 | 181 | 168 | 260 | – | 192 | 237 | 82 | 91 | 35 | 95 | 128 | 11 | 10 | 6 | 1 | 2 | 8 | 2 | 2 | 4 |
Endoplasmic Reticulum | 83 | 63 | 33 | 7 | 10 | 9 | 6 | – | 2 | 3 | 0 | 0 | 67 | 48 | 80 | 0 | 1 | 2 | 90 | 30 | 60 | 25 | 31 | 30 |
Endosome | 53 | 2 | 8 | 56 | 76 | 8 | 51 | – | 4 | 12 | 5 | 4 | 29 | 34 | 27 | 4 | 5 | 0 | 33 | 12 | 27 | 6 | 18 | 13 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 46 | 10 | 19 | 7 | 25 | 21 |
Mitochondria | 67 | 44 | 7 | 98 | 67 | 150 | 113 | – | 164 | 158 | 75 | 81 | 12 | 12 | 4 | 4 | 5 | 2 | 85 | 60 | 106 | 38 | 52 | 31 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 2 | 1 | – | 1 | 3 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 20 | 0 | 2 | 40 | 42 | 2 | 12 | – | 2 | 8 | 2 | 5 | 6 | 17 | 7 | 0 | 0 | 0 | 37 | 8 | 45 | 18 | 70 | 41 |
SpindlePole | 5 | 0 | 26 | 26 | 52 | 10 | 47 | – | 13 | 26 | 7 | 7 | 4 | 11 | 7 | 3 | 5 | 2 | 1 | 1 | 0 | 1 | 0 | 1 |
Vac/Vac Membrane | 38 | 2 | 13 | 29 | 63 | 12 | 44 | – | 21 | 20 | 6 | 5 | 10 | 5 | 7 | 4 | 2 | 1 | 9 | 6 | 6 | 2 | 2 | 2 |
Unique Cell Count | 279 | 118 | 189 | 341 | 451 | 322 | 484 | 353 | 440 | 163 | 183 | 152 | 214 | 255 | 30 | 35 | 20 | 411 | 179 | 382 | 151 | 329 | 280 | |
Labelled Cell Count | 338 | 148 | 215 | 411 | 523 | 386 | 553 | 427 | 498 | 189 | 221 | 180 | 252 | 284 | 33 | 44 | 23 | 411 | 179 | 382 | 151 | 329 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.8 | 5.9 | 5.6 | 6.1 | 4.3 | 4.9 | – | 4.1 | 4.3 | 4.2 | 4.2 | 6.2 | 5.6 | 6.1 | 6.7 | 6.9 | 7.3 | 5.2 | 5.3 | 5.9 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.4 | 1.4 | 1.8 | 1.2 | 1.1 | – | 0.9 | 1.1 | 1.5 | 1.0 | 1.4 | 1.6 | 1.4 | 1.9 | 0.9 | 1.7 | 1.2 | 0.8 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.04 | -0.47 | -0.28 | – | -0.52 | -0.44 | -0.48 | -0.48 | 0.06 | -0.06 | 0.04 | 0.18 | 0.23 | 0.31 | -0.19 | -0.15 | 0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.5081 | 0.1902 | -0.2798 | -0.57 | -0.4002 | -0.3535 | 1.9041 | 1.7796 | 1.6794 | 1.0343 | 1.716 | 1.6341 | 3.1614 | 4.4367 | 4.044 | 3.7311 | 4.0058 | 3.8482 |
Actin | 0.0875 | 0.0519 | 0.0829 | 0.0687 | 0.0914 | 0.0532 | 0.0842 | 0.0622 | 0.0665 | 0.0803 | 0.0525 | 0.0506 | 0.0868 | 0.1077 | 0.0874 | 0.0934 | 0.1367 | 0.067 |
Bud | 0.0011 | 0.0006 | 0.0011 | 0.0003 | 0.0008 | 0.0008 | 0.0005 | 0.0008 | 0.0012 | 0.0006 | 0.0018 | 0.0004 | 0.0002 | 0.0012 | 0.0003 | 0.0004 | 0.0002 | 0.0002 |
Bud Neck | 0.0027 | 0.0017 | 0.0051 | 0.0311 | 0.0018 | 0.0078 | 0.0025 | 0.0023 | 0.0122 | 0.0152 | 0.003 | 0.0078 | 0.0015 | 0.0017 | 0.0032 | 0.0258 | 0.0026 | 0.0073 |
Bud Periphery | 0.0011 | 0.0007 | 0.0013 | 0.0005 | 0.001 | 0.0011 | 0.0008 | 0.0017 | 0.0049 | 0.0011 | 0.0027 | 0.0007 | 0.0004 | 0.0014 | 0.0005 | 0.0008 | 0.0003 | 0.0005 |
Bud Site | 0.0094 | 0.007 | 0.0071 | 0.0012 | 0.0088 | 0.001 | 0.0057 | 0.0197 | 0.0132 | 0.0015 | 0.0031 | 0.0012 | 0.0021 | 0.0152 | 0.0058 | 0.0069 | 0.0009 | 0.0005 |
Cell Periphery | 0.0029 | 0.0025 | 0.0017 | 0.0007 | 0.0005 | 0.0013 | 0.0017 | 0.003 | 0.004 | 0.001 | 0.0015 | 0.0015 | 0.0033 | 0.0018 | 0.0028 | 0.0027 | 0.001 | 0.0024 |
Cytoplasm | 0.0989 | 0.1132 | 0.0755 | 0.0621 | 0.0748 | 0.1053 | 0.1163 | 0.1598 | 0.142 | 0.154 | 0.0579 | 0.1381 | 0.1079 | 0.1921 | 0.1114 | 0.1198 | 0.1083 | 0.1412 |
Cytoplasmic Foci | 0.1044 | 0.1331 | 0.1147 | 0.1252 | 0.1087 | 0.1218 | 0.1077 | 0.1312 | 0.1037 | 0.1136 | 0.1073 | 0.1104 | 0.1197 | 0.1129 | 0.1016 | 0.1552 | 0.1524 | 0.0945 |
Eisosomes | 0.0023 | 0.0007 | 0.0015 | 0.0009 | 0.0006 | 0.0007 | 0.001 | 0.0013 | 0.0013 | 0.0006 | 0.0009 | 0.0006 | 0.0025 | 0.0007 | 0.0022 | 0.0021 | 0.0011 | 0.0017 |
Endoplasmic Reticulum | 0.1623 | 0.1156 | 0.1403 | 0.1011 | 0.0528 | 0.1258 | 0.1635 | 0.1905 | 0.2101 | 0.1272 | 0.1574 | 0.1927 | 0.2092 | 0.1586 | 0.2138 | 0.1708 | 0.0735 | 0.2313 |
Endosome | 0.141 | 0.1699 | 0.1622 | 0.1691 | 0.1418 | 0.1557 | 0.1287 | 0.1215 | 0.1314 | 0.1769 | 0.146 | 0.1436 | 0.0922 | 0.0924 | 0.1007 | 0.0741 | 0.0855 | 0.0808 |
Golgi | 0.0702 | 0.1358 | 0.0827 | 0.0723 | 0.1509 | 0.0664 | 0.0567 | 0.0594 | 0.0643 | 0.0688 | 0.0722 | 0.0636 | 0.0517 | 0.0751 | 0.0601 | 0.0516 | 0.0548 | 0.0512 |
Lipid Particles | 0.1479 | 0.1343 | 0.1447 | 0.197 | 0.2399 | 0.1621 | 0.1373 | 0.1331 | 0.1054 | 0.0558 | 0.1307 | 0.1449 | 0.2167 | 0.1257 | 0.1546 | 0.0985 | 0.0965 | 0.1618 |
Mitochondria | 0.0195 | 0.0094 | 0.0218 | 0.0187 | 0.043 | 0.0269 | 0.0106 | 0.0141 | 0.0208 | 0.0244 | 0.1363 | 0.0167 | 0.0043 | 0.0047 | 0.0079 | 0.0077 | 0.004 | 0.0103 |
None | 0.0743 | 0.0487 | 0.0729 | 0.07 | 0.0046 | 0.0771 | 0.0903 | 0.0534 | 0.0574 | 0.1134 | 0.0489 | 0.0474 | 0.0662 | 0.0808 | 0.1023 | 0.1212 | 0.2186 | 0.0949 |
Nuclear Periphery | 0.0294 | 0.0361 | 0.0178 | 0.0239 | 0.017 | 0.0337 | 0.0381 | 0.0172 | 0.0153 | 0.0224 | 0.0171 | 0.0278 | 0.0132 | 0.0065 | 0.0109 | 0.0084 | 0.0079 | 0.0202 |
Nucleolus | 0.0023 | 0.0004 | 0.0019 | 0.0003 | 0.0006 | 0.0005 | 0.0008 | 0.0018 | 0.0006 | 0.0002 | 0.0006 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0002 |
Nucleus | 0.007 | 0.0026 | 0.0089 | 0.0022 | 0.0009 | 0.0036 | 0.0099 | 0.0018 | 0.0025 | 0.0018 | 0.0063 | 0.0058 | 0.001 | 0.0014 | 0.0011 | 0.0012 | 0.0011 | 0.0015 |
Peroxisomes | 0.0169 | 0.0233 | 0.0334 | 0.0333 | 0.0498 | 0.033 | 0.0233 | 0.0118 | 0.026 | 0.022 | 0.039 | 0.0298 | 0.0123 | 0.0124 | 0.0243 | 0.0507 | 0.0483 | 0.0219 |
Punctate Nuclear | 0.0085 | 0.0043 | 0.0124 | 0.0116 | 0.0042 | 0.0104 | 0.012 | 0.0034 | 0.0068 | 0.0071 | 0.0033 | 0.0066 | 0.0042 | 0.003 | 0.0032 | 0.0044 | 0.0038 | 0.005 |
Vacuole | 0.0073 | 0.0058 | 0.007 | 0.0056 | 0.0035 | 0.0075 | 0.0055 | 0.0076 | 0.0081 | 0.0091 | 0.0074 | 0.0067 | 0.0035 | 0.0036 | 0.0044 | 0.003 | 0.0019 | 0.0038 |
Vacuole Periphery | 0.0031 | 0.0024 | 0.003 | 0.0041 | 0.0025 | 0.0044 | 0.0029 | 0.0022 | 0.0024 | 0.003 | 0.0041 | 0.0028 | 0.0012 | 0.0009 | 0.0014 | 0.0011 | 0.0007 | 0.0017 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.7468 | 3.1018 | 5.7542 | 13.5604 | 11.247 | 5.2019 | 7.4486 | 9.5254 | 7.7345 | 12.9131 |
Translational Efficiency | 0.6788 | 2.0163 | 1.2015 | 0.5689 | 0.566 | 1.641 | 0.9655 | 0.5296 | 0.9402 | 0.6763 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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