Standard name
Human Ortholog
Description Haploid specific endoprotease of a-factor mating pheromone; performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.34 0.38 0.34 0.23 0.16 0.21 0.07 0.05 0 0 0 0 0.37 0.27 0.18 0.23 0.21 0.23 0.12 0.09 0.14 0.07 0.09 0.1
Bud 0.12 0.16 0.2 0.16 0.19 0.21 0.15 0.18 0.14 0.18 0.21 0.09 0.1 0.07 0.15 0.05 0 0.05 0.22 0.16 0.17 0.33 0.35 0.25
Bud Neck 0.12 0 0.18 0.14 0.19 0.13 0.1 0.09 0.07 0.06 0.09 0 0 0.06 0 0.07 0.11 0 0.52 0.53 0.49 0.42 0.24 0.13
Bud Site 0.46 0.4 0.37 0.43 0.26 0.29 0.22 0.15 0.16 0.15 0.17 0.1 0.2 0.19 0.16 0.1 0.08 0.12 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.16 0.15 0.14 0.24 0.23 0.22 0.24 0.34 0.24 0.26 0.26 0.29 0.32 0.43 0.35 0.3 0.34 0 0.05 0 0.1 0.15 0.33
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.06 0.07 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.38 0.35 0.37 0.19 0.36 0.48 0.49 0.54 0.68 0.59 0.64 0.14 0 0.06 0.06 0 0.09 0 0 0 0 0 0
Nucleus 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.16 0.08 0.05 0.15 0.09 0.07 0.07 0.05 0.06 0.06 0 0 0.31 0.33 0.21 0.1 0.1 0.23 0 0 0 0 0.05 0
SpindlePole 0.21 0 0.05 0.17 0.14 0.05 0.14 0.17 0.08 0.06 0.07 0.06 0.09 0.16 0.06 0.3 0.28 0.25 0 0 0 0 0 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 128 51 48 34 27 59 30 18 2 10 3 6 38 34 24 29 22 25 54 15 22 4 13 17
Bud 45 22 29 23 32 57 63 70 27 43 50 27 10 9 20 6 4 6 99 27 27 22 53 43
Bud Neck 46 6 26 20 31 35 42 35 14 14 21 12 4 7 3 9 12 4 241 88 79 28 37 21
Bud Site 173 54 52 63 44 80 91 58 31 36 40 32 21 23 21 13 9 13 – – – – – –
Cell Periphery 3 3 2 2 2 0 0 3 1 3 3 5 4 1 3 1 1 1 3 1 0 0 0 2
Cytoplasm 43 22 21 21 40 64 92 91 65 57 63 80 30 40 56 45 32 37 10 8 5 6 23 57
Endoplasmic Reticulum 4 2 2 0 5 1 5 1 1 1 0 2 1 3 2 7 3 6 12 1 1 0 1 1
Endosome 7 0 0 1 0 0 0 0 0 0 0 0 5 7 5 7 8 2 4 4 7 0 3 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 0 1 0 0 0 0 0
Mitochondria 9 51 49 55 31 100 203 185 102 163 141 199 15 1 8 8 2 10 3 6 6 1 3 3
Nucleus 38 0 4 1 2 2 15 17 7 5 8 7 0 1 2 1 4 4 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 1 0 3 0 0 3 0 0 0 0 0 0 0 3 1 0 0 0 0
Nucleolus 4 0 1 0 0 0 0 3 1 0 1 1 1 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 60 11 7 22 15 18 30 19 11 15 6 10 32 41 28 13 11 25 11 1 3 1 7 1
SpindlePole 78 6 7 25 24 15 60 65 16 15 18 18 9 20 8 38 30 27 3 2 2 1 6 10
Vac/Vac Membrane 3 2 0 0 2 1 1 0 0 2 0 3 0 0 0 2 2 3 6 5 4 1 1 2
Unique Cell Count 380 136 142 148 167 275 423 381 190 241 240 309 104 124 131 127 107 110 461 168 163 68 154 172
Labelled Cell Count 642 230 248 267 255 433 632 568 278 364 357 402 170 187 180 183 142 163 461 168 163 68 154 172


Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 3.9 3.6 3.5 3.8 3.3 3.0 3.2 2.9 2.7 2.7 2.6 5.1 5.5 4.7 7.5 8.2 7.9 4.4 5.0 5.3
Std Deviation (1e-4) 0.7 0.6 0.6 0.5 1.1 0.8 0.7 1.0 1.2 0.9 1.0 0.9 0.9 1.8 1.5 2.6 2.8 2.6 0.4 1.9 1.7
Intensity Change (Log2) – – – -0.04 0.07 -0.12 -0.25 -0.19 -0.31 -0.4 -0.4 -0.49 0.5 0.61 0.4 1.06 1.19 1.14 0.3 0.46 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1772 1.7058 1.2325 1.0899 0.6429 1.5965 1.4029 2.2432 1.6275 1.3273 1.2624 1.8009 2.0489 2.6268 2.5575 2.3545 3.2131 2.557
Actin 0.0745 0.0451 0.0738 0.0015 0.0086 0.0731 0.1223 0.0636 0.0792 0.0051 0.0104 0.0548 0.0735 0.0457 0.0432 0.0064 0.0234 0.0611
Bud 0.0011 0.0069 0.0012 0.0001 0.0009 0.0004 0.0017 0.002 0.0016 0.0001 0.0009 0.0007 0.0015 0.006 0.0011 0.0004 0.0003 0.0004
Bud Neck 0.4443 0.0455 0.6025 0.9945 0.9127 0.8893 0.3093 0.041 0.542 0.8933 0.8535 0.8955 0.2635 0.0465 0.5867 0.9615 0.9061 0.9132
Bud Periphery 0.0012 0.0101 0.0024 0.0002 0.0017 0.0009 0.0022 0.0044 0.0031 0.0002 0.001 0.0023 0.0027 0.0109 0.0024 0.0009 0.0006 0.0011
Bud Site 0.3659 0.6839 0.1958 0.0014 0.0281 0.0194 0.3114 0.7341 0.2242 0.0028 0.027 0.0036 0.4712 0.6641 0.2692 0.0283 0.0047 0.0035
Cell Periphery 0.0034 0.0117 0.0049 0.0004 0.0013 0.0011 0.0029 0.0087 0.0054 0.0004 0.0008 0.0022 0.0057 0.0096 0.0065 0.0008 0.0019 0.0017
Cytoplasm 0.042 0.0961 0.0323 0.0003 0.0034 0.002 0.031 0.0276 0.0219 0.0006 0.0024 0.0085 0.0159 0.1098 0.0266 0.0003 0.0033 0.014
Cytoplasmic Foci 0.0139 0.017 0.0185 0.0006 0.0149 0.0028 0.0442 0.0046 0.0077 0.0007 0.0269 0.0069 0.0267 0.0177 0.0057 0.0005 0.0017 0.0014
Eisosomes 0.0018 0.0006 0.0012 0 0.0001 0.0004 0.0011 0.0035 0.0009 0.0054 0.0002 0.0005 0.0025 0.001 0.0012 0.0001 0.0024 0.0004
Endoplasmic Reticulum 0.0002 0.0025 0.0005 0 0.0001 0 0.0114 0.0005 0.0011 0 0.0001 0.0002 0.005 0.0026 0.0005 0 0.0001 0.0001
Endosome 0.0001 0.0033 0.0018 0 0.0008 0.0001 0.0231 0.0002 0.0134 0 0.024 0.0018 0.0117 0.0028 0.0003 0 0.0001 0.0001
Golgi 0.0006 0.0011 0.001 0.0001 0.0019 0.0003 0.0084 0.0008 0.0057 0.0001 0.0229 0.0023 0.0048 0.0019 0.0009 0.0001 0.0003 0.0003
Lipid Particles 0.0002 0.0017 0.0011 0 0.0026 0.0001 0.0153 0.0005 0.006 0 0.012 0.0023 0.0095 0.0019 0.0012 0 0.0001 0
Mitochondria 0.0001 0.0006 0.0002 0 0.0003 0 0.008 0.0006 0.0077 0 0.0039 0.0029 0.0048 0.0016 0.0003 0 0.0001 0
None 0.0481 0.0643 0.058 0.0002 0.0012 0.0074 0.0526 0.0963 0.0625 0.0905 0.0022 0.0092 0.0725 0.0677 0.0441 0 0.0511 0.002
Nuclear Periphery 0 0.001 0.0001 0 0 0 0.0129 0.0002 0.0025 0 0.0001 0.0001 0.0056 0.0007 0.0001 0 0 0
Nucleolus 0.0004 0.0008 0.0004 0.0001 0.0037 0.0004 0.0009 0.0004 0.001 0.0001 0.0004 0.0025 0.0014 0.0012 0.0009 0.0002 0.0014 0.0001
Nucleus 0.0003 0.0021 0.0008 0.0001 0.0028 0.0004 0.0023 0.0007 0.0029 0.0001 0.0009 0.0004 0.0011 0.0021 0.0008 0.0001 0.0011 0.0001
Peroxisomes 0.0008 0.0014 0.0016 0 0.0126 0.0002 0.0268 0.0082 0.0046 0.0002 0.0087 0.0022 0.0096 0.0027 0.0068 0.0001 0.0002 0.0002
Punctate Nuclear 0.0007 0.0022 0.0013 0.0002 0.0013 0.0016 0.0089 0.0017 0.0051 0.0002 0.0006 0.0004 0.0087 0.0018 0.0012 0.0001 0.0009 0.0001
Vacuole 0.0002 0.0019 0.0003 0 0.001 0.0001 0.0017 0.0003 0.001 0 0.0009 0.0006 0.0012 0.0017 0.0004 0.0001 0.0001 0.0002
Vacuole Periphery 0 0.0001 0 0 0 0 0.0013 0 0.0004 0 0.0003 0.0001 0.0009 0.0001 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.9519 10.3342 8.2739 17.0958 13.2135 9.1215 11.6176 16.1461 12.9813 16.6105
Translational Efficiency 0.3279 0.3198 0.5437 0.2239 0.2564 0.4071 0.3668 0.2403 0.3264 0.26

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Haploid specific endoprotease of a-factor mating pheromone; performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
Localization
Cell Percentages bud neck (19%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Axl1

Axl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Axl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available