| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 1 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 |
| Bud Neck | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Bud Site | – | 0 | – | – | 1 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | – | 1 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | – | 32 | – | – | 5 | – | – | – | – | – | – | – | – | – | – | 106 | 67 | 129 | 18 | 71 | 2 | 2 | 0 | 4 |
| Endoplasmic Reticulum | – | 1 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 2 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
| Endosome | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Golgi | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| Mitochondria | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 5 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 |
| Nucleus | – | 0 | – | – | 10 | – | – | – | – | – | – | – | – | – | – | 38 | 14 | 9 | 2 | 0 | 6 | 1 | 5 | 3 |
| Nuclear Periphery | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | – | 0 | – | – | 6 | – | – | – | – | – | – | – | – | – | – | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
| Peroxisomes | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | – | 0 | – | – | 0 | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | 0 | – | – | 6 | – | – | – | – | – | – | – | – | – | – | 6 | 3 | 3 | 0 | 2 | 4 | 0 | 0 | 1 |
| Unique Cell Count | 33 | 26 | 154 | 85 | 144 | 24 | 81 | 17 | 6 | 10 | 12 | |||||||||||||
| Labelled Cell Count | 34 | 28 | 161 | 88 | 155 | 24 | 81 | 17 | 6 | 10 | 12 | |||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | 48.8 | – | – | 12.0 | – | – | – | – | – | – | – | – | – | – | 40.5 | 55.2 | 72.0 | – | – | – |
| Std Deviation (1e-4) | – | 17.7 | – | – | 4.0 | – | – | – | – | – | – | – | – | – | – | 16.9 | 18.9 | 22.5 | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | 0.0244 | 0.0003 | 0.0036 | 0 | 0.0085 | 0.0061 | 0.0141 | 0.0002 | 0.0004 | 0.0007 | 0.0069 | 0.0008 | 0.0029 | 0.0118 | 0.0008 | 0.2255 | 0.0006 | 0.0059 |
| Bud | 0.0038 | 0.0004 | 0.0018 | 0.0004 | 0.0153 | 0.0033 | 0.0013 | 0.0002 | 0.0005 | 0.0011 | 0.0014 | 0.0035 | 0.0006 | 0.0016 | 0.0007 | 0.004 | 0.0001 | 0.0006 |
| Bud Neck | 0.0007 | 0.0003 | 0.0006 | 0.0001 | 0.0021 | 0.004 | 0.0019 | 0.0007 | 0.0015 | 0.0019 | 0.0036 | 0.0031 | 0.0005 | 0.0005 | 0.0014 | 0.0045 | 0.0002 | 0.0011 |
| Bud Periphery | 0.0042 | 0.0001 | 0.0012 | 0 | 0.0091 | 0.0049 | 0.0011 | 0.0001 | 0.0001 | 0.0003 | 0.002 | 0.002 | 0.0005 | 0.001 | 0.0003 | 0.0048 | 0 | 0.0011 |
| Bud Site | 0.0021 | 0.0003 | 0.0008 | 0 | 0.0181 | 0.0005 | 0.0055 | 0.0003 | 0.0004 | 0.0003 | 0.0008 | 0.0004 | 0.0002 | 0.0028 | 0.0003 | 0.0105 | 0.0001 | 0.0001 |
| Cell Periphery | 0.003 | 0.0013 | 0.0032 | 0.0002 | 0.012 | 0.0014 | 0.0066 | 0.003 | 0.0049 | 0.0023 | 0.0006 | 0.0034 | 0.0081 | 0.0032 | 0.0007 | 0.0015 | 0 | 0.0003 |
| Cytoplasm | 0.7085 | 0.9716 | 0.6952 | 0.9627 | 0.8405 | 0.8859 | 0.7367 | 0.9853 | 0.9547 | 0.969 | 0.8549 | 0.8522 | 0.8567 | 0.9627 | 0.9311 | 0.6474 | 0.9965 | 0.8201 |
| Cytoplasmic Foci | 0.0105 | 0.0005 | 0.03 | 0.0007 | 0.0099 | 0.0069 | 0.0054 | 0.0005 | 0.0009 | 0.001 | 0.001 | 0.0038 | 0.0008 | 0.0006 | 0.0016 | 0.0079 | 0.0006 | 0.001 |
| Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0039 | 0 | 0.0001 |
| Endoplasmic Reticulum | 0.0229 | 0.0012 | 0.0197 | 0.0005 | 0.0039 | 0.0055 | 0.0295 | 0.0011 | 0.0016 | 0.0004 | 0.0239 | 0.0053 | 0.0204 | 0.002 | 0.0242 | 0.0049 | 0.0001 | 0.0306 |
| Endosome | 0.0297 | 0.0004 | 0.0329 | 0.0016 | 0.0081 | 0.0129 | 0.0093 | 0.0005 | 0.001 | 0.0006 | 0.0231 | 0.0101 | 0.0049 | 0.0006 | 0.0028 | 0.0104 | 0.0002 | 0.0215 |
| Golgi | 0.0031 | 0.0001 | 0.007 | 0 | 0.0007 | 0.0014 | 0.0017 | 0.0001 | 0 | 0.0001 | 0.0015 | 0.0006 | 0.0006 | 0.0004 | 0.0002 | 0.0101 | 0 | 0.006 |
| Lipid Particles | 0.0036 | 0.0001 | 0.0026 | 0 | 0.0029 | 0.0002 | 0.0023 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0006 | 0.0006 | 0.0001 | 0.0004 | 0.0021 | 0 | 0.0003 |
| Mitochondria | 0.0071 | 0.0001 | 0.0879 | 0 | 0.0023 | 0.0099 | 0.0063 | 0 | 0.0001 | 0.0003 | 0.0068 | 0.0011 | 0.0017 | 0.0015 | 0.0003 | 0.0496 | 0 | 0.0331 |
| None | 0.0107 | 0.0001 | 0.0006 | 0.0001 | 0.0044 | 0.0009 | 0.0139 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0257 | 0.0004 | 0.0018 | 0.0009 | 0.0001 | 0.0006 |
| Nuclear Periphery | 0.0501 | 0.0017 | 0.0094 | 0.003 | 0.0047 | 0.0135 | 0.0722 | 0.0013 | 0.0033 | 0.0019 | 0.045 | 0.0156 | 0.027 | 0.0007 | 0.0043 | 0.0021 | 0.0003 | 0.0527 |
| Nucleolus | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0023 | 0.0004 | 0.0106 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0009 | 0.0002 | 0.0003 | 0 | 0.0001 |
| Nucleus | 0.018 | 0.0018 | 0.003 | 0.0015 | 0.0032 | 0.0113 | 0.038 | 0.0019 | 0.0036 | 0.0044 | 0.009 | 0.0104 | 0.0063 | 0.0022 | 0.003 | 0.0025 | 0.0006 | 0.0033 |
| Peroxisomes | 0.0005 | 0 | 0.0028 | 0 | 0.0006 | 0.0003 | 0.0004 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0021 | 0 | 0 |
| Punctate Nuclear | 0.0023 | 0 | 0.001 | 0 | 0.0005 | 0.0002 | 0.0041 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0019 | 0 | 0.0002 | 0.0007 | 0 | 0.0002 |
| Vacuole | 0.0685 | 0.0172 | 0.0782 | 0.0236 | 0.0407 | 0.0188 | 0.0314 | 0.0042 | 0.0253 | 0.0124 | 0.0113 | 0.0671 | 0.0354 | 0.0061 | 0.0203 | 0.0027 | 0.0005 | 0.0064 |
| Vacuole Periphery | 0.025 | 0.0023 | 0.0183 | 0.0053 | 0.0099 | 0.0119 | 0.0073 | 0.0002 | 0.0012 | 0.0023 | 0.0066 | 0.019 | 0.0048 | 0.0007 | 0.0053 | 0.0016 | 0.0001 | 0.0149 |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 2350.4026 | 3119.0205 | 3977.1739 | 2815.6189 | 3298.9 | 2892.9448 | 2547.9577 | 2359.7846 | 3128.606 | 2378.1074 |
| Translational Efficiency | 1.6614 | 1.3571 | 1.0804 | 1.8339 | 1.4636 | 1.4355 | 1.5604 | 1.6043 | 1.2624 | 1.5997 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | ✘ |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal |
| Localization | |
| Cell Percentages | cytoplasm (96%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | cytoplasm-2 |
Rps23b
Rps23b
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Rps23b-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
