DUB Biology is an online resource that contains information about the changes elicited at the yeast proteome in different single and double KO mutants of key deubiquitination enzymes (DUBs) (Suresh et al. 2024). To retrieve this information, a single-cell imaging pipeline that exploits the yeast protein-green fluorescent protein (GFP) collection was used. The protein-GFP collection is a resource consisting of thousands of strains expressing different proteins tagged C-terminally with GFP, at their endogenous loci. The global GFP protein fusion library was crossed into ubp2Δ, ubp14Δ, and ubp2Δ ubp14Δ mutants, and the recombinant progeny alongside respective wild-type strains were examined for changes in both protein localization and abundance, using live-cell high-throughput confocal microscopy and automated image analysis. The resource contains both the raw images of the project, as well as data that were extracted from their analysis.
At the main page of the project (https://thecellvision.org/dubbiology), the user types their protein of interest. Here, either Yeast gene names or the names of their Human orthologues can be used, and multiple genes can be queried at the same time. The user is then directed to the respective protein(s) page, where cell images of the mutant(s) – along with wild type controls – expressing endogenous fusions of the respective protein with GFP can be browsed and directly downloaded. The quantified abundance and localization of the protein in each of the genetic backgrounds (alongside the respective differences among them) are also displayed in tabular format below. The tables can be also downloaded via the respective prompt.