About the Project

The Endocytic Compartment Morphology dataset includes images from high-throughput screens of yeast single-gene mutants combined with single cell analysis of subcellular, endocytic compartment morphology, obtained using neural networks, as described in Mattiazzi Usaj et al. (Mattiazzi Usaj et al. 2020). Endocytic Compartment Morphology images are single-channel maximum z-projections acquired via confocal microscopy, depicting the subcellular morphology of 4 endocytic markers in 5,628 yeast mutant strains (corresponding to 5,267 S. cerevisiae ORFs). The screened mutant strains include non-essential gene deletion strains, and strains with temperature-sensitive alleles of essential genes. All temperature-sensitive strains were screened at two temperatures (26° and 37°). The identification of significant morphology mutants only included temperature-sensitive strains screened at the non-permissive temperature (37°). However, all screens done at the permissive temperature (26°) are included in TheCellVision.org together with micrographs, and results from penetrance and phenotype analyses.

Penetrance Results
  • penetrance = percentage of the population with an aberrant phenotype
  • FP = fluorescent protein
  • SD = standard deviation
  • penetrance mutant
    • = is not a penetrance mutant
    • = is a penetrance mutant
  • NOTE: images are scaled individually to the maximum intensity in each image
Specific Phenotype Results
  • SD = standard deviation
  • * membrane = localization of Snf7 to the vacuolar membrane
  • specific phenotype mutant (SPM)
    • = not an SPM
    • = SPM
    • ✔✔ = stringent SPM
  • Markers
    • Actin patch (Sac6)

    • Cortical patch (Sla1)

    • Late endosome (Snf7)

    • Vacuole (Vph1)

Download results
  • Legend
    • actin: Actin patch
    • coat: Cortical patch
    • LE: Late endosome
    • vac: Vacuole
    • gw: Genome wide screen
    • secondary: secondary screen
    • SMF: Single mutant fitness
    • SD: Standard deviation
  • Columns
    • A-G: strain information
    • H-AM: p-value, penetrance, cell count and well count data for each marker and screen type
    • AN-BG: total cell count, total well count, combined mean penetrance, penetrance SD data for each marker
    • BH-DE: data on phenotype fractions for each phenotype and screen type
    • DF-GK: combined phenotype fraction, and phenotype fraction SD, (stringent) SPM calls for each phenotype (Note: TSA strains grown at room temperature (RT) were not scored for SPMs/stringent SPMs and thus have no value in columns *_SPM, *_stringent SPM (* = compartment)