| YAL003W |
EFB1 |
eEF1Balpha | EF-1beta | TEF5 | translation elongation factor 1 subunit beta |
EEF1B2| EEF1D |
Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site; human homolog EEF1B2 can complement yeast efb1 mutants |
| YAL025C |
MAK16 |
ribosome biosynthesis protein MAK16 |
MAK16 |
Essential nuclear protein; constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus; contains an Fe/S cluster that functions as a redox sensor and is required for Mak16p-Rpf1p complex formation and ribosome biogenesis |
| YAL034W-A |
MTW1 |
DSN3 | MIND complex subunit MTW1 | NSL2 |
MIS12 |
Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND) |
| YAL038W |
CDC19 |
PYK1 | pyruvate kinase CDC19 |
PKM |
Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication |
| YBL023C |
MCM2 |
MCM DNA helicase complex subunit MCM2 |
MCM2 |
Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; binds and transfers parental H3-H4 to Pol32p facilitating the transfer of parental histones to the lagging strand via Pol1p during DNA replication; relative distribution to the nucleus increases upon DNA replication stress |
| YBL034C |
STU1 |
— |
CLASP2 |
MT plus-end-tracking non-motor protein; binds MTs, stabilizes interpolar MT plus ends, facilitates spindle MT polymerization and produces an outward force on spindle poles; required for mitotic spindle integrity; binds detached kinetochores (KTs) and upon phosphorylation by Mps1p recruits Slk19p, promoting capture and clustering; regulates spindle stability; relocalizes to MTs when captured KTs reach a spindle pole; localizes to spindles, spindles poles, and detached KTs; human CLASP1/2 homolog |
| YBL035C |
POL12 |
DNA-directed DNA polymerase alpha subunit POL12 |
POLA2 |
B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation |
| YBL050W |
SEC17 |
alpha-soluble NSF attachment protein SEC17 | RNS3 |
NAPA| NAPB |
Alpha-SNAP cochaperone; SNARE-complex adaptor for Sec18 (NSF) during the disassembly of postfusion cis-SNARE complexes; stimulates the ATPase activity of Sec18p; peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; similar to mammalian alpha-SNAP |
| YBL076C |
ILS1 |
isoleucine--tRNA ligase ILS1 |
IARS1 |
Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A |
| YBL084C |
CDC27 |
anaphase promoting complex subunit CDC27 | APC3 | SNB1 |
CDC27 |
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition |
| YBL093C |
ROX3 |
MED19 | NUT3 | RMR1 | SSN7 | SSX2 |
— |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme |
| YBL097W |
BRN1 |
condensin subunit BRN1 |
NCAPH |
Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission |
| YBL105C |
PKC1 |
CLY15 | CLY5 | CLY7 | HPO2 | protein kinase C | RSF8 | STT1 |
PRKCE| PRKCD| PRKCG| PRKCI| PKN1| PRKCA| PRKCB| PKN2| PKN3| PRKCH| PRKCQ| PRKCZ |
Protein serine/threonine kinase; essential for cell wall remodeling during growth; phosphorylates Ssa1p in response to the Mid2p mechanosensor, regulating the heat shock response, including epichaperome rearrangement and fine-tuning of multiple cellular processes including ribosome engagement, translational fidelity and P-body disassembly; localizes to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) |
| YBR011C |
IPP1 |
inorganic diphosphatase IPP1 | PPA1 |
PPA1| PPA2 |
Cytoplasmic inorganic pyrophosphatase (PPase); homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase |
| YBR029C |
CDS1 |
CDG1 | phosphatidate cytidylyltransferase |
CDS2| CDS1 |
Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids; human homolog CDS1 can complement yeast cds1 null mutant |
| YBR055C |
PRP6 |
RNA6 | TSM7269 | U4/U6-U5 snRNP complex subunit PRP6 |
PRPF6 |
Splicing factor; component of the U4/U6-U5 snRNP complex |
| YBR060C |
ORC2 |
origin recognition complex subunit 2 | RRR1 | SIR5 |
ORC2 |
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p |
| YBR070C |
ALG14 |
N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase anchoring subunit ALG14 |
ALG14 |
Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; localizes to the peroxisome; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant; mutations of human ALG14 cause congenital myasthenic syndrome |
| YBR079C |
RPG1 |
TIF32 | translation initiation factor eIF3 core subunit a |
EIF3A |
eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; essential for loading mRNA onto ribosome at entry and exit channels of preinitiation complex; involved in translation reinitiation; eIF3 is also involved in programmed stop codon readthrough |
| YBR080C |
SEC18 |
AAA family ATPase SEC18 | ANU4 |
NSF |
AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF) |
| YBR102C |
EXO84 |
exocyst subunit EXO84 | USA3 |
EXOC8 |
Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting of the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate |
| YBR109C |
CMD1 |
calmodulin | CaM |
CALML3| CALM1| CALM3| CALM2 |
Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability |
| YBR110W |
ALG1 |
chitobiosyldiphosphodolichol beta-1,4 mannosyltransferase |
ALG1L1P| ALG1L2| ALG1 |
Mannosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability; human homolog ALG1 complements yeast null mutant |
| YBR135W |
CKS1 |
cyclin-dependent protein kinase regulatory subunit CKS1 |
CKS2| CKS1B |
Cyclin-dependent kinase regulatory subunit; recognizes TP phosphosites, and functions as a phosphoadaptor that anchors Cdc28p-cyclin complexes onto their substrates, providing specificity and processivity; required for G1/S and G2/M phase transitions; mediates Sic1p phosphorylation and destruction at S phase onset and proteolysis of M-phase targets; regulates transcription by recruiting the proteasome to target gene promoters; human homologs CKS1B and CKS2 each complement the cks1 null mutant |
| YBR143C |
SUP45 |
eRF1 | SAL4 | SUP1 | SUP47 | translation termination factor eRF1 |
ETF1 |
Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p |
| YBR154C |
RPB5 |
ABC27 | DNA-directed RNA polymerase core subunit RPB5 |
POLR2E |
RNA polymerase subunit ABC27; common to RNA polymerases I, II, and III; contacts DNA and affects transactivation |
| YBR155W |
CNS1 |
HSP70/90 family co-chaperone CNS1 |
TTC4 |
TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70); stimulates ATPase activity of Ssa1p; ts mutants reduce Hsp82p function, overexpression suppresses phenotypes of HSP82 ts allele and cpr7 deletion; involved in the folding of eukaryotic elongation factor 2 (Eft1p, Eft2p); human homolog TTC4 complements yeast cns1 mutant |
| YBR156C |
SLI15 |
— |
— |
Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p |
| YBR160W |
CDC28 |
CDK1 | cyclin-dependent serine/threonine-protein kinase CDC28 | HSL5 | SRM5 |
CDK1| CDK2| CDK3 |
Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant |
| YBR193C |
MED8 |
RNA polymerase II mediator complex subunit MED8 |
— |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
| YBR196C |
PGI1 |
CDC30 | glucose-6-phosphate isomerase |
GPI |
Glycolytic enzyme phosphoglucose isomerase; catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation |
| YBR211C |
AME1 |
ARP100 |
— |
Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress |
| YBR234C |
ARC40 |
— |
ARPC1B| ARPC1A |
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches |
| YBR247C |
ENP1 |
MEG1 | snoRNA-binding rRNA-processing protein ENP1 |
BYSL |
Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus; human BYSL functionally complements the heat sensitivity of an enp1 ts mutant |
| YBR254C |
TRS20 |
TRAPP subunit TRS20 |
TRAPPC2B| TRAPPC2 |
Core component of transport protein particle (TRAPP) complexes I-III; TRAPPs are multimeric guanine nucleotide-exchange factors for GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); mutation leads to defects in endocytic recycling, block in sporulation/meiosis; mutations in human homolog TRAPPC2 cause spondyloepiphyseal dysplasia tarda, TRAPPC2 can complement yeast null mutant |
| YBR265W |
TSC10 |
3-dehydrosphinganine reductase |
KDSR |
3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family; mutations in human homolog KDSR cause recessive progressive symmetric erythrokeratoderma |
| YCR002C |
CDC10 |
septin CDC10 |
SEPTIN9| SEPTIN12| SEPTIN3 |
Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress |
| YCR052W |
RSC6 |
— |
SMARCD2| SMARCD3| SMARCD1 |
Component of the RSC chromatin remodeling complex; essential for mitotic growth; RSC6 has a paralog, SNF12, that arose from the whole genome duplication |
| YDL003W |
MCD1 |
kleisin alpha | PDS3 | RHC21 | SCC1 |
RAD21L1| RAD21 |
Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis; subject to proteolytic cleavage by separase Esp1p, resulting in dissociation of cohesin from chromatin and the separation of sister chromatids at the mitotic metaphase-to-anaphase transition; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase |
| YDL007W |
RPT2 |
proteasome regulatory particle base subunit RPT2 | YHS4 | YTA5 |
PSMC1 |
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle; N-myristoylation of Rpt2p at Gly2 is involved in regulating the proper intracellular distribution of proteasome activity by controlling the nuclear localization of the 26S proteasome |
| YDL008W |
APC11 |
anaphase promoting complex subunit 11 |
ANAPC11 |
Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity |
| YDL028C |
MPS1 |
PAC8 | RPK1 | serine/threonine/tyrosine protein kinase MPS1 |
TTK |
Dual-specificity kinase; required for SPB duplication and mitotic and meiotic spindle checkpoints; triggers formation of force-generating kinetochore-microtubule (MT) attachments to promote kinetochore bi-orientation in MI; activation loop autophosphorylation enhances kinase activity; substrates include SPB proteins (Spc42p, Spc110p, Spc98p), MEN protein Mob1p, kinetochore protein Cnn1p, checkpoint protein Mad1p, and MT stabilization factor Stu1p; substrate of APCC(Cdh1); similar to human TTK |
| YDL029W |
ARP2 |
ACT2 | actin-related protein 2 |
ACTR2 |
Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; required for efficient Golgi-to-ER trafficking in COPI mutants |
| YDL030W |
PRP9 |
SF3a splicing factor complex subunit PRP9 |
SF3A3 |
Subunit of the SF3a splicing factor complex; required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex |
| YDL043C |
PRP11 |
RNA11 | spliceosome assembly protein PRP11 |
SF3A2 |
Subunit of the SF3a splicing factor complex; required for spliceosome assembly |
| YDL045C |
FAD1 |
FMN adenylyltransferase |
FLAD1 |
Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin; mutation is functionally complemented by human FLAD1 |
| YDL064W |
UBC9 |
E2 SUMO-conjugating protein UBC9 |
UBE2I |
SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC); auto-sumoylates on K153 and K157 |
| YDL084W |
SUB2 |
ATP-dependent RNA helicase SUB2 |
DDX39B| DDX39A |
Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress |
| YDL097C |
RPN6 |
NAS4 | proteasome regulatory particle lid subunit RPN6 |
PSMD11 |
Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress |
| YDL105W |
NSE4 |
QRI2 | Smc5-Smc6 complex subunit NSE4 |
NSMCE4A| EID3 |
Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair |
| YDL108W |
KIN28 |
TFIIH complex serine/threonine-protein kinase subunit KIN28 |
CDK7 |
Ser/Thr protein kinase and subunit of TFIIK, a TFIIH subassembly; phosphorylates the C-terminal domain (CTD) of RNAPII (Ser5 and Ser7); facilitates recruitment of mRNA 5'-capping and polyadenylation factors to the RNAPII holoenzyme complex; minor role in RNAPII transcription; role in RNAPI transcription; regulates Atg1p kinase complex assembly during nitrogen starvation induced autophagy; associates with cyclin Ccl1p; relocalizes to the cytosol in response to hypoxia; ortholog of human CDK7 |
| YDL126C |
CDC48 |
AAA family ATPase CDC48 |
VCP |
AAA ATPase with protein-unfoldase activity; subunit of polyUb-selective segregase complex involved in ERAD, EGAD, INMAD, the RADAR pathway, mitotic spindle disassembly, macroautophagy, PMN, ribosome-associated quality control, ribophagy, homotypic ER membrane fusion, SCF complex disassembly, cell wall integrity, and telomerase regulation; mobilizes membrane-anchored transcription factors by regulated Ub/proteasome-dependent processing (RUP); human ortholog VCP complements a cdc48 mutant |
| YDL132W |
CDC53 |
cullin CDC53 |
CUL1| CUL2 |
Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant |
| YDL145C |
COP1 |
coatomer subunit alpha | RET1 | SEC33 | SOO1 |
COPA |
Alpha subunit of COPI vesicle coatomer complex; complex surrounds transport vesicles in the early secretory pathway |
| YDL147W |
RPN5 |
NAS5 | proteasome regulatory particle lid subunit RPN5 |
PSMD12 |
Subunit of the CSN and 26S proteasome lid complexes; similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein; the COP9 signalosome is also known as the CSN |
| YDL165W |
CDC36 |
CCR4-NOT core subunit CDC36 | DNA19 | NOT2 |
CNOT2 |
Component of the CCR4-NOT core complex, involved in mRNA decapping; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs through deadenylation; basal transcription factor |
| YDL195W |
SEC31 |
WEB1 |
SEC31A| SEC31B |
Component of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance |
| YDL207W |
GLE1 |
BRR3 | NLE2 | nucleoporin GLE1 | RSS1 |
GLE1 |
Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination |
| YDL209C |
CWC2 |
active spliceosome conformation promoter CWC2 | NTC40 |
— |
Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 |
| YDR002W |
YRB1 |
CST20 | HTN1 | Ran GTPase-binding protein YRB1 | SFO1 | STP52 |
RANBP1 |
Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1 |
| YDR013W |
PSF1 |
CDC101 | DNA replication protein PSF1 |
GINS1 |
Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery |
| YDR016C |
DAD1 |
— |
— |
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis |
| YDR044W |
HEM13 |
coproporphyrinogen oxidase |
CPOX |
Coproporphyrinogen III oxidase; oxygen-requiring enzyme that catalyzes sixth step in heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p); human homolog CPOX can complement yeast mutant and allow growth of haploid null after sporulation of a heterozygous diploid |
| YDR050C |
TPI1 |
triose-phosphate isomerase TPI1 |
TPI1 |
Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human homolog TPI1 causes a rare autosomal disease; human TPI1 can complement yeast null mutant |
| YDR052C |
DBF4 |
DNA52 | LSD7 | protein serine/threonine kinase activating protein DBF4 |
DBF4| DBF4B |
Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress; co-expression of human CDC7 and DBF4 complements single cdc7 or dbf4 null mutations or the cdc7 dbf4 double null mutation |
| YDR054C |
CDC34 |
DNA6 | SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 | UBC3 |
UBE2G1| UBE2R2| CDC34 |
Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functionally complements the thermosensitivity of the cdc34-2 mutant |
| YDR060W |
MAK21 |
NOC1 | RNA-binding ribosome biosynthesis protein MAK21 |
CEBPZ |
Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein |
| YDR064W |
RPS13 |
40S ribosomal protein uS15 RPS13 | RPS13B | RPS13C | S13 | S15 | S27a | uS15 | YS15 |
RPS13 |
Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 |
| YDR082W |
STN1 |
— |
— |
Telomere end-binding and capping protein; plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping; similar to human Stn1 |
| YDR091C |
RLI1 |
Fe-S cluster-binding ribosome biosynthesis protein |
ABCE1 |
Essential Fe-S protein; required for ribosome biogenesis, translation initiation/termination; facilitates binding of multifactor complex (MFC) of initiation factors to small ribosomal subunit; Dom34-Hbs1 complex and Rli1p work in dissociating inactive ribosomes, thereby facilitating translation restart; forms complex with Lto1p and Yae1p; dependency on ROS-labile FeS clusters, activity in nuclear ribosomal-subunit export impaired by mild oxidative stress |
| YDR113C |
PDS1 |
securin |
— |
Securin; inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation |
| YDR118W |
APC4 |
anaphase promoting complex subunit 4 |
ANAPC4 |
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress |
| YDR141C |
DOP1 |
— |
DOP1B| DOP1A |
Protein involved in vesicular transport at trans-Golgi network (TGN); TGN-localized, leucine-zipper domain protein; involved in endosome-to-Golgi transport during endocytic recycling, and retrograde transport of glycosyltransferases from the TGN to the Golgi; involved in organization of the ER, establishment of cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies |
| YDR145W |
TAF12 |
TAF61 | TAF68 | TafII61 | TafII68 |
TAF12 |
Subunit (61/68 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A; overexpression of the human ortholog, TAF12, an oncogene involved in the formation of choroid plexus carcinomas, results in dosage chromosomal instability (dCIN) in a human cell line similar to the dCIN observed in yeast overexpressors |
| YDR164C |
SEC1 |
— |
STXBP1| STXBP2| STXBP3 |
Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function; interacts directly with essential exocyst subunit Sec6p |
| YDR166C |
SEC5 |
exocyst subunit SEC5 |
EXOC2 |
Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip |
| YDR168W |
CDC37 |
Hsp90 co-chaperone CDC37 | SMO1 |
CDC37L1| CDC37 |
Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding |
| YDR170C |
SEC7 |
Arf family guanine nucleotide exchange factor SEC7 |
ARFGEF2| ARFGEF1 |
Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors; involved in intra-Golgi transport, ER-to-Golgi transport and proliferation of the Golgi; role in directing misfolded proteins to insoluble deposition sites and detoxifying neurological disease proteins; found in the cytoplasm and on Golgi-associated coated vesicles |
| YDR180W |
SCC2 |
cohesin-loading factor complex subunit SCC2 |
NIPBL |
Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; promotes gene expression program that supports translational fidelity; evolutionarily-conserved adherin; relocalizes to cytosol in response to hypoxia; human disorder Cornelia de Lange syndrome is caused by mutations in NIPBL, the human ortholog of SCC2; conserved role in NHEJ |
| YDR182W |
CDC1 |
DSC1 | DSR1 | ESP2 | putative lipid phosphatase CDC1 |
MPPE1 |
Putative mannose-ethanolamine phosphate phosphodiesterase; involved in GPI-anchor remodeling prior to the attachment of cell wall proteins to beta 1,3-glucan, removing ethanolamine phosphate from the first mannose of GPI anchors; mutants display elevated Ca2+-dependent signaling resulting in secondary actin polarization and Golgi inheritance defects; enzyme is Mn2+-dependent; mutants have cell division cycle defect and fragile cell walls |
| YDR189W |
SLY1 |
syntaxin-binding protein |
SCFD1 |
Hydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex |
| YDR196C |
CAB5 |
putative dephospho-CoA kinase |
DCAKD |
Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; involved in histone acylation; null mutant lethality is complemented by human homologs DCAKD and COASY and by E. coli coaE (encoding DPCK); extrinsic component of the mitochondrial outer membrane that also localizes to lipid droplets |
| YDR201W |
SPC19 |
— |
— |
Essential subunit of Dam1 complex (aka DASH complex); complex couples kinetochores to force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body; SPC19 mRNA level is reduced when downstream gene RAV2 is deleted |
| YDR228C |
PCF11 |
— |
PCF11 |
mRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia |
| YDR288W |
NSE3 |
Smc5-Smc6 complex subunit NSE3 |
MAGEB16| MAGEB17| MAGEA9B| MAGEA10| MAGEA8| MAGEA4| MAGEB4| MAGEB5| MAGEB3| MAGEB6B| MAGEA3| MAGEC2| MAGEA2B| MAGEA6| MAGEH1| MAGEA11| MAGEB18| MAGEB1| MAGEB6| MAGEB2| MAGEF1| MAGEA1| NDN| MAGEB10| MAGEA2| MAGEA13P| NSMCE3| MAGEA9| MAGEE2| MAGEA12 |
Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress |
| YDR292C |
SRP101 |
signal recognition particle receptor subunit alpha |
SRPRA |
Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p |
| YDR302W |
GPI11 |
mannose-ethanolamine phosphotransferase GPI11 |
PIGF |
ER membrane protein involved in a late step of GPI anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated glycosylphosphatidylinositol (GPI) intermediate; human PIG-Fp is a functional homolog |
| YDR303C |
RSC3 |
— |
— |
Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; RSC3 has a paralog, RSC30, that arose from the whole genome duplication |
| YDR328C |
SKP1 |
CBF3D | MGO1 | SCF ubiquitin ligase subunit SKP1 |
SKP1 |
Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress |
| YDR331W |
GPI8 |
GPI-anchor transamidase |
PIGK |
Catalytic subunit of the ER membrane GPI transamidase complex; involved in adding glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; luminally-oriented type I integral membrane glycoprotein of the ER; human PIGK protein is a functional homolog |
| YDR361C |
BCP1 |
protein-transporting protein BCP1 |
BCCIP |
Essential nuclear chaperone for ribosomal protein Rpl23p; involved in nuclear export of Mss4p; Mss4p is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport |
| YDR394W |
RPT3 |
proteasome regulatory particle base subunit RPT3 | YNT1 | YTA2 |
PSMC4 |
ATPase of the 19S regulatory particle of the 26S proteasome; one of many regulatory particle ATPases; involved in the degradation of ubiquitinated substrates; enhances proteasomal activity and upregulates Hac1p in response to proteotoxic stress; substrate of N-acetyltransferase B |
| YDR404C |
RPB7 |
B16 | DNA-directed RNA polymerase II subunit RPB7 |
POLR2G |
RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation |
| YDR434W |
GPI17 |
GPI-anchor transamidase GPI17 |
PIGS |
Transmembrane protein; subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog |
| YDR437W |
GPI19 |
phosphatidylinositol N-acetylglucosaminyltransferase GPI19 |
PIGP |
Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis; shares similarity with mammalian PIG-P |
| YDR489W |
SLD5 |
CDC105 | DNA replication protein SLD5 |
GINS4 |
Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery |
| YDR498C |
SEC20 |
— |
BNIP1 |
Membrane glycoprotein v-SNARE; involved in retrograde transport from the Golgi to the endoplasmic reticulum (ER); required for N- and O-glycosylation in the Golgi but not in the ER and for efficient nuclear fusion during mating; mediates Sey1p-independent homotypic ER fusion; interacts with the Dsl1p complex through Tip20p |
| YDR510W |
SMT3 |
SUMO family protein SMT3 |
SUMO3| SUMO2| SUMO1P1| SUMO1 |
Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; human homolog SUMO1 can complement yeast null mutant |
| YDR527W |
RBA50 |
— |
RPAP1 |
Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 |
| YDR531W |
CAB1 |
pantothenate kinase |
PANK1| PANK3| PANK2| PANK4 |
Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase; cytoplasmic enzyme that catalyzes the first committed step in the universal biosynthetic pathway for coenzyme A (CoA) synthesis; transcription is regulated by Upc2p via a sterol response element; overexpressed human homologs, PANK1 and PANK3, complement yeast hypomorphic alleles, while PANK2, a gene implicated in pantothenate-kinase associated neurodegeneration, partially complements after the mitochondrial transit peptide is removed |
| YEL034W |
HYP2 |
eIF5A | eIF-5A | TIF51A | translation elongation factor eIF-5A |
EIF5A| EIF5AL1| EIF5A2 |
Translation elongation factor eIF-5A; required for translation of proteins with polyproline stretches; hypusination promotes translation elongation, termination and ribosomal frameshifting; localizes to the nucleus and binds chromatin to repress transcription; structural homolog of bacterial EF-P; human EIF5A variants cause a disorder with variable combinations of developmental delay, microcephaly, micrognathia and dysmorphism; human EIF5A complements inviability of a hyp2 anb1 double null |
| YEL055C |
POL5 |
DNA-directed DNA polymerase |
MYBBP1A |
Protein involved in 60S assembly and pre-40S AFs recycling; has sequence similarity to human MybBP1A and weak sequence similarity to B-type DNA polymerases; not required for chromosomal DNA replication |
| YER009W |
NTF2 |
Ran GTPase-binding protein NTF2 |
NUTF2 |
Nuclear envelope protein; interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport |
| YER022W |
SRB4 |
MED17 |
MED17 |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination |
| YER093C |
TSC11 |
AVO3 | TORC2 complex subunit TSC11 |
RICTOR |
Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; TORC2 also regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; contains an N-terminal armadillo repeat region necessary for recruitment to the PM and for anchoring Tor2p and Avo1p at the PM; acts along with Avo1p as a scaffold important for integrity of the TORC2 complex; involved in sphingolipid metabolism; contains a RasGEFN domain |
| YER094C |
PUP3 |
proteasome core particle subunit beta 3 | SCS32 |
PSMB3 |
Beta 3 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit C10 |
| YER125W |
RSP5 |
HPG1 | MDP1 | MUT2 | NEDD4 family E3 ubiquitin-protein ligase | NPI1 | SMM1 | UBY1 |
NEDD4L| WWP2| NEDD4 |
NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p, Sem1p, Dip5p, Put4p and Chs3p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome |
| YER133W |
GLC7 |
CID1 | DIS2 | DIS2S1 | PP1 | type 1 serine/threonine-protein phosphatase catalytic subunit GLC7 |
PPP1CC| PPP1CB| PPP1CA |
Type 1 S/T protein phosphatase (PP1) catalytic subunit; involved in glycogen metabolism, sporulation and mitotic progression; interacts with multiple regulatory subunits; regulates actomyosin ring formation; subunit of CPF; recruited to mating projections by Afr1p interaction; regulates nucleocytoplasmic shuttling of Hxk2p; import into the nucleus is inhibited during spindle assembly checkpoint arrest; involved in dephosphorylating Rps6a/b and Bnr1p |
| YER136W |
GDI1 |
SEC19 |
GDI2| GDI1 |
GDP dissociation inhibitor; regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins |
| YER157W |
COG3 |
Golgi transport complex subunit COG3 | GRD20 | SEC34 |
COG3 |
Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments |
| YER171W |
RAD3 |
REM1 | TFIIH/NER complex ATP-dependent 5'-3' DNA helicase subunit RAD3 |
ERCC2 |
5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress |
| YFL005W |
SEC4 |
Rab family GTPase SEC4 | SRO6 |
RAB13| RAB8A| RAB10| RAB8B |
Rab family GTPase; essential for vesicle-mediated exocytic secretion and autophagy; recruits Avl9p, an Arf-GAP, to secretory vesicles; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane; exocyst association is negatively regulated via phosphorylation by Cdc5p during cytokinesis |
| YFL009W |
CDC4 |
SCF ubiquitin ligase complex subunit CDC4 |
FBXW7 |
F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p |
| YFL022C |
FRS2 |
phenylalanine--tRNA ligase subunit alpha |
FARSA |
Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar |
| YFL029C |
CAK1 |
CIV1 | cyclin-dependent protein kinase-activating kinase CAK1 |
— |
Cyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases; required for premeiotic DNA synthesis, expression of early and middle sporulation specific genes and direct phosphorylation of the Smk1p MAPK to regulate spore morphogenesis |
| YFL038C |
YPT1 |
Rab family GTPase YPT1 | YP2 |
RAB1C| RAB1B| RAB1A |
Rab family GTPase; involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by type II protein geranylgeranyltransferase (Bet2p-Bet4p); binds to unspliced HAC1 mRNA; regulates the unfolded protein response (UPR) by promoting the decay of HAC1 RNA; localizes to the early Golgi, the transitional Golgi and ER membranes, pre-autophagosomal structures, and cytoplasmic vesicles |
| YFL039C |
ACT1 |
ABY1 | actin | END7 |
POTEKP| ACTC1| ACTA1| ACTB| ACTG1| ACTA2| ACTBL2| ACTG2 |
Actin, structural protein in cytoskeleton; involved in cell polarization, endocytosis, and other cytoskeletal functions; monomeric actin in the nucleus plays a role in INO80 chromatin remodeling |
| YFR004W |
RPN11 |
MPR1 | proteasome regulatory particle lid subunit RPN11 |
PSMD14 |
Metalloprotease subunit of the 19S regulatory particle; part of 26S proteasome lid; couples deubiquitination and degradation of proteasome substrates; allosteric ubiquitin sensor that slows spontaneous conformational transitions of the proteasome and aids substrate insertion into the ATPase motor to accelerate degradation up to 4-fold; involved in the fission of mitochondria and peroxisomes independent of catalytic activity; protein abundance increases in response to DNA replication stress |
| YFR028C |
CDC14 |
OAF3 | phosphoprotein phosphatase CDC14 |
CDC14A| CDC14B| CDC14C |
Protein phosphatase required for mitotic exit; required for rDNA segregation, cytokinesis, meiosis I spindle disassembly, environmental stress response, autophagy during meiosis; downregulates microautophagy induction after TORC1 inactivation; held in nucleolus by Cdc55p in early meiosis, liberated by FEAR and Mitotic Exit Network in anaphase; sequestered in metaphase II, released upon entry into anaphase II; human homolog CDC14A can complement thermosensitivity of cdc14-1 mutant |
| YFR036W |
CDC26 |
anaphase promoting complex subunit CDC26 | HIT3 | SCD26 |
— |
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia |
| YFR050C |
PRE4 |
proteasome core particle subunit beta 7 |
PSMB4 |
Beta 7 subunit of the 20S proteasome |
| YFR051C |
RET2 |
coatomer subunit delta |
ARCN1 |
Delta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER |
| YFR052W |
RPN12 |
NIN1 | proteasome regulatory particle lid subunit RPN12 |
PSMD8 |
Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress |
| YGL047W |
ALG13 |
N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase catalytic subunit ALG13 |
ALG13 |
Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress; both human homologs ALG13 and ALG14 are required to complement yeast alg13 mutant |
| YGL048C |
RPT6 |
CIM3 | CRL3 | proteasome regulatory particle base subunit RPT6 | SCB68 | SUG1 |
PSMC5 |
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress |
| YGL061C |
DUO1 |
— |
— |
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p and Pse1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis |
| YGL065C |
ALG2 |
GDP-Man:Man(1)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase |
ALG2 |
Mannosyltransferase in the N-linked glycosylation pathway; catalyzes two consecutive steps in N-linked glycosylation pathway; mutants exhibit temperature-sensitive growth and abnormal accumulation of lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol; mutations of human ALG2 cause congenital myasthenic syndrome; human ALG2 complements temperature sensitivity and dolichol-linked oligosaccharide biosynthesis defect of alg2-1 mutant, but mutant form from a patient with CDG-Ii fails to complement |
| YGL073W |
HSF1 |
EXA3 | MAS3 | stress-responsive transcription factor HSF1 |
HSF1| HSF2| HSF4 |
Trimeric heat shock transcription factor; forms condensate with Mediator and RNA Pol II upon heat shock; activates multiple genes in response to diverse stresses and transient intracellular acidification; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status through RQC (Ribosomal Quality Control)-mediated translation-stress signal; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant |
| YGL075C |
MPS2 |
MMC1 |
— |
Essential membrane protein localized at nuclear envelope and SPBs; required for insertion of the newly duplicated spindle pole body into the nuclear envelope; potentially phosphorylated by Cdc28p; MPS2 has a paralog, CSM4, that arose from the whole genome duplication |
| YGL092W |
NUP145 |
nucleocytoplasmic transporter NUP145 | RAT10 |
NUP98 |
Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98 |
| YGL097W |
SRM1 |
MTR1 | PRP20 | Ran guanyl-nucleotide exchange factor | TSM437 |
RCC1 |
Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant |
| YGL098W |
USE1 |
SLT1 | SNAP receptor USE1 |
USE1 |
Essential SNARE protein localized to the ER; involved in retrograde traffic from the Golgi to the ER and Sey1p-independent homotypic ER fusion; required for efficient nuclear fusion during mating; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p |
| YGL113W |
SLD3 |
— |
— |
Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with the DDK-phosphorylated MCM complex and required for the recruitment of Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress; homologous to the human Treslin/Ticrr protein |
| YGL116W |
CDC20 |
PAC5 | ubiquitin-protein transferase activating protein CDC20 |
CDC20B| CDC20 |
Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress |
| YGL120C |
PRP43 |
DEAH-box ATP-dependent RNA helicase PRP43 | JA1 |
DHX32| DHX15 |
RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p |
| YGL130W |
CEG1 |
mRNA guanylyltransferase |
RNGTT |
Guanylyltransferase involved in mRNA 5' capping; subunit of mRNA capping enzyme, which is a heterotetramer composed of two molecules of Ceg1p and a homodimer of Cet1p, the mRNA 5'-triphosphatase subunit; nuclear import of Ceg1p requires interaction with Cet1p; mammalian capping enzyme is a single bifunctional polypeptide; human homolog RNGTT can complement yeast ceg1 null mutant |
| YGL137W |
SEC27 |
coatomer subunit beta' |
COPB2 |
Essential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP |
| YGL142C |
GPI10 |
putative glycosylphosphatidylinositol-alpha 1,2 mannosyltransferase |
PIGB |
Integral membrane protein involved in GPI anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the glycosylphosphatidylinositol (GPI) core structure; human PIG-Bp is a functional homolog |
| YGL145W |
TIP20 |
TIP1 |
RINT1 |
Peripheral membrane protein required for COPI vesicle fusion to the ER; mediates Sey1p-independent homotypic ER fusion; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p |
| YGL233W |
SEC15 |
Rab GTPase-binding exocyst subunit SEC15 |
EXOC6| EXOC6B |
Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase |
| YGL245W |
GUS1 |
GluRS | glutamate--tRNA ligase GUS1 | GSN1 |
— |
Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress |
| YGL247W |
BRR6 |
— |
— |
Essential nuclear envelope (NE) integral membrane protein; role in NE fusion during biogenesis of the nuclear pore complex; Brr6p-Brl1p interaction proposed to drive membrane fusion by forming a channel across the bilayers enabling lipid exchange; interacts and functions with Apq12p and Brl1p in lipid homeostasis; role in maintaining architecture and transcriptional regulation at the NE through chromatin interaction; Brl1p homolog; functional homolog of metazoan CLCC1 |
| YGR002C |
SWC4 |
EAF2 | GOD1 |
DMAP1 |
Component of the Swr1p complex that incorporates Htz1p into chromatin; component of the NuA4 histone acetyltransferase complex; associates with and protects nucleosome-free regions of the genome including rDNA, tDNA and telomeric loci, contributing to genome integrity |
| YGR006W |
PRP18 |
mRNA splicing protein PRP18 |
PRPF18 |
Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p |
| YGR009C |
SEC9 |
HSS7 |
SNAP25| SNAP23 |
t-SNARE protein required for secretory vesicle-plasma membrane fusion; similar to but not functionally redundant with Spo20p; interacts non-exocyst bound Sec6p; SNAP-25 homolog |
| YGR048W |
UFD1 |
polyubiquitin-binding protein UFD1 |
UFD1 |
Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in the RADAR pathway; involved in mobilizing membrane bound transcription factors by regulated Ub/proteasome-dependent processing (RUP) |
| YGR075C |
PRP38 |
U4/U6-U5 snRNP complex subunit PRP38 |
— |
Unique component of the U4/U6.U5 tri-snRNP particle; tri-snRNP is required for conformational changes which result in the catalytic activation of the spliceosome; dispensable for spliceosome assembly |
| YGR091W |
PRP31 |
U4/U6-U5 snRNP complex subunit PRP31 |
PRPF31 |
Splicing factor; component of the U4/U6-U5 snRNP complex; homologous to human PRPF31 and used to study retinitis pigmentosa |
| YGR092W |
DBF2 |
serine/threonine-protein kinase DBF2 |
— |
Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication |
| YGR098C |
ESP1 |
separase |
ESPL1 |
Separase/separin, a caspase-like cysteine protease; cleaves Mcd1p/Scc1p, a mitotic cohesin complex subunit, resulting in the dissociation of cohesin from chromatin and sister chromatid separation; cleaves meiotic-cohesin subunit Rec8p along chromosome arms in meiosis I and at centromeric sites during meiosis II; inhibits PP2A-Cdc55p to promote mitotic exit; inhibited by Pds1p (securin); relative distribution to the nucleus increases upon DNA replication stress |
| YGR113W |
DAM1 |
— |
— |
Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p and Pse1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization, thereby aiding in chromosome segregation; N-terminal staple region is important for self-assembly of the Dam1 complex higher order ring structure that forms around microtubules; Ipl1p target for regulating kinetochore-MT attachments |
| YGR119C |
NUP57 |
FG-nucleoporin NUP57 |
NUP54 |
FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) |
| YGR120C |
COG2 |
Golgi transport complex subunit COG2 | SEC35 |
— |
Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; the components of the Golgi complex are Gog1p through Cog8p |
| YGR140W |
CBF2 |
CBF3A | CEP2 | CSL5 | CTF14 | NDC10 |
— |
Essential kinetochore protein and subunit of the CBF3 complex (CBF3c); CBF3c binds as a dimer to the CDEIII region of the centromere (CEN) and to pericentromeric DNA; Cbf2p trimerization, essential for kinetochore assembly and chromosome segregation, facilitates CEN DNA bending and may stabilize the centromeric nucleosome and inner kinetochore; ubiquitinated in vivo and sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress |
| YGR172C |
YIP1 |
transporter YIP1 |
YIPF5| YIPF7 |
Integral membrane protein; required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; human homolog YIPF5 can complement yeast yip1 mutant |
| YGR185C |
TYS1 |
TTS1 | tyrosine--tRNA ligase TYS1 | TyrRS |
YARS1 |
Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; human ortholog YARS functionally complements the heat sensitivity of a ts allele; protein abundance increases in response to DNA replication stress |
| YGR211W |
ZPR1 |
zinc finger-containing protein ZPR1 |
ZPR1 |
Chaperone with two zinc fingers essential for eEF1A biogenesis; nuclear in growing cells, cytoplasmic in starved cells; shuttled via process mediated by Cpr1p; binds translation elongation factor eEF-1 (Tef1p); relative distribution to nucleus increases upon DNA replication stress; human ZPR1 gene can complement yeast by allowing growth during down-regulation of yeast zpr1 |
| YGR216C |
GPI1 |
phosphatidylinositol N-acetylglucosaminyltransferase |
PIGQ |
Membrane protein involved in the synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1 are functional homologs |
| YGR218W |
CRM1 |
exportin CRM1 | KAP124 | XPO1 |
XPO1 |
Nuclear export factor, exportin; major karyopherin involved in export of proteins, RNAs, and ribosomal subunits from the nucleus |
| YGR253C |
PUP2 |
DOA5 | proteasome core particle subunit alpha 5 |
PSMA5 |
Alpha 5 subunit of the 20S proteasome; involved in ubiquitin-dependent catabolism; human homolog is subunit zeta |
| YGR264C |
MES1 |
MESI | methionine--tRNA ligase MES1 | MetRS |
MARS1 |
Methionyl-tRNA synthetase; forms a complex with Gus1p, a glutamyl-tRNA synthetase, and Arc1p, which increases the catalytic efficiency of both synthetases; involved in the nuclear export of tRNAs; mutations in human ortholog MARS is associated with pediatric pulmonary alveolar proteinosis, interstitial lung and liver disease, and Charcot-Marie-Tooth disease; human MARS gene can complement the yeast null mutant |
| YHR024C |
MAS2 |
MIF2 | mitochondrial-processing protease subunit alpha |
PMPCA |
Alpha subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins |
| YHR027C |
RPN1 |
HRD2 | NAS1 | proteasome regulatory particle base subunit RPN1 |
PSMD2 |
Non-ATPase base subunit of the 19S RP of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions; RP is the acronym for regulatory particle |
| YHR036W |
BRL1 |
— |
— |
Essential nuclear envelope/ER integral membrane protein; role in nuclear envelope fusion during biogenesis of the nuclear pore complex ; Brl1p-Brr6p interaction proposed to drive membrane fusion by forming a channel across the bilayers enabling lipid exchange; interacts and functions with Apq12p and Brr6p in lipid homeostasis; identified as a dosage suppressor of a conditional mutation in the major karyopherin, CRM1; Brr6p homolog; functional homolog of metazoan CLCC1 |
| YHR058C |
MED6 |
mediator complex subunit MED6 |
MED6 |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress |
| YHR083W |
SAM35 |
FMP20 | SAM complex subunit SAM35 | TOB38 | TOM38 |
— |
Component of the sorting and assembly machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane |
| YHR088W |
RPF1 |
rRNA-binding ribosome biosynthesis protein RPF1 |
RPF1 |
Protein involved in assembly and export of the large ribosomal subunit; nucleolar protein; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA |
| YHR107C |
CDC12 |
CLA10 | PSL7 | septin CDC12 |
SEPTIN2 |
Septin ring component required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that associate to form rod shaped protofilaments that polymerize end-to-end forming filaments; mother-bud neck localized septin rings act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; C-terminal amphipathic helix senses membrane curvature tuned by oligomerization |
| YHR118C |
ORC6 |
origin recognition complex subunit 6 |
— |
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; mutation in the human Orc6p is linked to Meier-Gorlin syndrome |
| YHR122W |
CIA2 |
iron-sulfur cluster assembly protein CIA2 |
CIAO2B |
Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation and maturation of a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B |
| YHR166C |
CDC23 |
anaphase promoting complex subunit CDC23 |
CDC23 |
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition |
| YHR172W |
SPC97 |
gamma-tubulin-complex subunit SPC97 |
TUBGCP2 |
Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque |
| YHR186C |
KOG1 |
LAS24 | ubiquitin-binding TORC1 subunit KOG1 |
RPTOR |
Ubiquitin-binding protein; centrally regulates metabolic homeostasis and carbon utilization during nutrient limitation by managing Snf1p activity; subunit of TORC1 rapamycin-sensitive complex involved in growth control; central scaffold that holds the different components of TORC1 together; contains four HEAT repeats and seven WD-40 repeats |
| YHR188C |
GPI16 |
GPI-anchor transamidase subunit GPI16 |
PIGT |
Subunit of the glycosylphosphatidylinositol transamidase complex; involved in the addition of GPIs to newly synthesized proteins; luminally-oriented, type I integral membrane glycoprotein of the ER; human PIG-T homolog |
| YIL004C |
BET1 |
SLY12 |
BET1L| BET1 |
Type II membrane protein required for vesicular transport; required for vesicular transport between the endoplasmic reticulum and Golgi complex; v-SNARE with similarity to synaptobrevins |
| YIL021W |
RPB3 |
B44 | DNA-directed RNA polymerase II core subunit RPB3 |
POLR2C |
RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit |
| YIL046W |
MET30 |
ubiquitin-binding SDF ubiquitin ligase complex subunit MET30 | ZRG11 |
FBXW7 |
Nuclear F-box protein and subunit of SCF-Met30 Ub-ligase complex; controls the cell cycle, sulfur metabolism, and methionine biosynthesis as part of SCF-Met30 Ub-ligase complex; sulfide-sensor that represses activity of Met4p in response to sulfur abundance; involved in turnover of Cdk-inhibitory kinase Swe1p; Cdc48p catalyzes dissociation of Met30p from SCF complex in response to cadmium stress; contains WD40 repeat region with cysteines that sense sulfur availability |
| YIL048W |
NEO1 |
aminophospholipid-translocating P4-type ATPase NEO1 |
ATP9A| ATP9B |
Phospholipid translocase (flippase); involved in generating phospholipid asymmetry in plasma membrane; involved in endocytosis, vacuolar biogenesis and Golgi-to-ER vesicle-mediated transport; localizes to endosomes and Golgi apparatus; targeted to vacuole via AP-3 pathway |
| YIL062C |
ARC15 |
— |
ARPC5L| ARPC5 |
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; has mRNA binding activity |
| YIL068C |
SEC6 |
SNARE-binding exocyst subunit SEC6 |
EXOC3| EXOC3L1| TNFAIP2 |
Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; binds to SNARE complexes binteracting with Sec9p |
| YIL106W |
MOB1 |
— |
MOB1A| MOB3B| MOB1B| MOB3A| MOB3C |
Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress |
| YIL109C |
SEC24 |
ANU1 | COPII subunit SEC24 |
SEC24A| SEC24B |
Component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SEC24 has a paralog, SFB2, that arose from the whole genome duplication |
| YIL115C |
NUP159 |
FG-nucleoporin NUP159 | NUP158 | RAT7 |
— |
FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) |
| YIR010W |
DSN1 |
MIND complex subunit DSN1 |
— |
Essential component of the outer kinetochore MIND complex; joins kinetochore subunits contacting DNA to those contacting microtubules; phosphorylation promotes interaction between outer and inner kinetochore proteins; kinetochore receptor for monopolin, via interaction with Csm1p; essential for both meiotic and mitotic chromosome segregation; MIND complex consists of Mtw1p, Nnf1p, Nsl1p and Dsn1p; phosphorylated by monopolin subunit, Hrr25p and Aurora kinase, Ipl1p; modified by sumoylation |
| YIR011C |
STS1 |
DBF8 | SSM5 |
— |
Karyopherin adaptor required for nuclear translocation of the proteasome; mediates interaction between nuclear import factor Srp1p and the proteasome; nuclear import of the 26S proteasome is coupled to RanGTP-initiated, ubiquitin-independent proteasomal degradation of Sts1p; Sts1p and Srp1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation; role in ubiquitin-mediated protein degradation; contains a bipartite nuclear localization sequence |
| YIR012W |
SQT1 |
— |
AAMP |
Specific assembly chaperone for ribosomal protein Rpl10p; co-translationally associates with nascent Rpl10p, preventing aggregation; involved in biogenesis of the ribosomal large subunit; contains multiple WD repeats; interacts genetically and physically with Qsr1p; protein abundance increases in response to DNA replication stress |
| YIR022W |
SEC11 |
signal peptidase complex catalytic subunit SEC11 |
SEC11C| SEC11A| SEC11B |
18kDa catalytic subunit of the Signal Peptidase Complex (SPC); the Signal Peptidase Complex cleaves the signal sequence of proteins targeted to the endoplasmic reticulum; other members are Spc1p, Spc2p, Spc3p, and Sec11p |
| YJL002C |
OST1 |
dolichyl-diphosphooligosaccharide--protein glycotransferase subunit OST1 | NLT1 |
RPN1 |
Alpha subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins |
| YJL005W |
CYR1 |
adenylate cyclase | CDC35 | FIL1 | HSR1 | SRA4 | TSM0185 |
PHLPP2| PHLPP1 |
Adenylate cyclase; required for cAMP production and cAMP-dependent protein kinase signaling; scaffold protein for Ras2-Ira interaction; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation |
| YJL019W |
MPS3 |
NEP98 | YJL018W |
— |
Nuclear envelope protein; required for SPB insertion, SPB duplication, Kar5p localization near the SPB and nuclear fusion; interacts with Mps2p to tether half-bridge to core SPB; N-terminal acetylation by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; member of the SUN protein family (Sad1-UNC-84 homology) |
| YJL034W |
KAR2 |
BiP | GRP78 | Hsp70 family ATPase KAR2 |
HSPA5 |
ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p |
| YJL035C |
TAD2 |
tRNA(adenine34) deaminase |
ADAT2 |
Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs |
| YJL039C |
NUP192 |
— |
NUP205 |
Essential subunit of inner ring of nuclear pore complex (NPC); plays a role in modulating transport through the NPC; homologous to human NUP205 |
| YJL072C |
PSF2 |
CDC102 | DNA replication protein PSF2 |
GINS2 |
Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery |
| YJL085W |
EXO70 |
GTP-Rho binding exocyst subunit EXO70 |
EXOC7 |
Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress |
| YJL097W |
PHS1 |
enoyl-CoA hydratase PHS1 |
HACD2| HACD1 |
Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking |
| YJL104W |
PAM16 |
import motor complex subunit PAM16 | MIA1 | TIM16 |
PAM16 |
Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); forms a 1:1 subcomplex with Pam18p and inhibits its cochaperone activity; contains a J-like domain |
| YJL125C |
GCD14 |
TRM61 | tRNA 1-methyladenosine methyltransferase subunit GCD14 |
TRMT61A |
Subunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression |
| YJL173C |
RFA3 |
RPA14 | RPA3 |
— |
Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein complex involved in DNA replication, repair, recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication stress |
| YJL194W |
CDC6 |
AAA family ATPase CDC6 |
CDC6 |
Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; dual role as a mitotic cyclin-dependent kinase inhibitor, that binds to and inhibits Cdc28p/Clb2p along with Sic1p, contributing to mitotic exit; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress; degraded in response to plasma membrane stress |
| YJL203W |
PRP21 |
SPP91 |
SF3A1 |
Subunit of the SF3a splicing factor complex; required for spliceosome assembly |
| YJR042W |
NUP85 |
RAT9 |
NUP85 |
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP85 aka NUP75 |
| YJR045C |
SSC1 |
ENS1 | Hsp70 family ATPase SSC1 | mtHSP70 |
HSPA9 |
Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication |
| YJR046W |
TAH11 |
CDT1 | SID2 |
— |
DNA replication licensing factor; required for pre-replication complex assembly |
| YJR057W |
CDC8 |
bifunctional thymidylate/uridylate kinase |
DTYMK |
Nucleoside monophosphate and nucleoside diphosphate kinase; functions in the de novo biosynthesis of pyrimidine deoxyribonucleotides; thymidylate/uridylate kinase that converts nucleoside monophosphates, dTMP and dUMP to nucleoside diphosphates, dTDP and dUDP; nucleoside diphosphate kinase that converts nucleoside diphosphates, dTDP and dUDP to dTTP and dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe tmp1; human homolog DTYMK can complement the cdc8 null mutant |
| YJR065C |
ARP3 |
ACT4 | actin-related protein 3 |
ACTR3B| ACTR3C| ACTR3 |
Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity |
| YJR076C |
CDC11 |
PSL9 | septin CDC11 |
SEPTIN5 |
Component of the septin ring that is required for cytokinesis; present at the ends of rod-like septin hetero-oligomers; C-terminal extension is important for recruitment of Bni5p to the mother-bud neck, which in turn is required for Myo1p recruitment and cytokinesis; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells |
| YJR112W |
NNF1 |
MIND complex subunit NNF1 |
PMF1| PMF1-BGLAP |
Essential component of the outer kinetochore MIND complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for kinetochore bi-orientation and accurate chromosome segregation; complex consists of Mtw1p, Nnf1p, Nsl1p and Dsn1p; homologous to metazoan CENP-H proteins |
| YKL006C-A |
SFT1 |
— |
— |
Intra-Golgi v-SNARE; required for transport of proteins between an early and a later Golgi compartment |
| YKL013C |
ARC19 |
— |
ARPC4 |
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; mutation is functionally complemented by human ARPC4 |
| YKL019W |
RAM2 |
bifunctional protein farnesyltransferase/protein geranylgeranyltransferase |
FNTA |
Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I; farnesyltransferase (Ram2p-Ram1p heterodimer) catalyzes the addition of 15-carbon isoprenoid farnesyl to substrate proteins containing a CAAX consensus motif; type I geranylgeranyltransferase (Ram2p-Cdc43p heterodimer) catalyzes the addition of the 20-carbon isoprenoid geranylgeranyl to substrate proteins containing a CaaL consensus motif; required for membrane localization of Ras proteins and a-factor |
| YKL021C |
MAK11 |
— |
PAK1IP1 |
Protein involved in an early step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats |
| YKL022C |
CDC16 |
anaphase promoting complex subunit CDC16 |
CDC16 |
Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia |
| YKL024C |
URA6 |
bifunctional uridylate/adenylate kinase | SOC8 |
CMPK1 |
Uridylate kinase; catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) |
| YKL035W |
UGP1 |
UTP glucose-1-phosphate uridylyltransferase |
UGP2 |
UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; involved in PKA-mediated oxidative stress resistance and long-term survival in stationary phase; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication |
| YKL052C |
ASK1 |
— |
— |
Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; sumoylated in an Mms21p-dependent manner; protein abundance increases in response to DNA replication stress |
| YKL060C |
FBA1 |
fructose-bisphosphate aldolase FBA1 | LOT1 |
— |
Fructose 1,6-bisphosphate aldolase; required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress; N-terminally propionylated in vivo |
| YKL088W |
CAB3 |
phosphopantothenoylcysteine decarboxylase complex subunit CAB3 |
PPCDC |
Subunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesis; subunits of the phosphopantothenoylcysteine decarboxylase (PPCDC) complex are: Cab3p, Sis2p, Vhs3p, while the subunits of the CoA-synthesizing protein complex (CoA-SPC) are: Cab2p, Cab3p, Cab4p, and Cab5p as well as Sis2p and Vhs3p; null mutant lethality is complemented by E. coli coaBC |
| YKL089W |
MIF2 |
— |
CENPC |
Centromeric CDEIII region binding protein; nucleates kinetochore assembly; required for the structural integrity of elongating spindles; copurifies with subunits of the MIND complex and centromeric nucleosome components (Cse4p and histones H2A, H2B, and H4); phosphorylated by Ipl1p; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-C and fission yeast cnp3; localizes to the kinetochore |
| YKL145W |
RPT1 |
CIM5 | proteasome regulatory particle base subunit RPT1 | YTA3 |
PSMC2 |
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance |
| YKL154W |
SRP102 |
Signal recognition particle receptor subunit beta |
SRPRB |
Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane |
| YKL165C |
MCD4 |
FSR2 | mannose-ethanolamine phosphotransferase MCD4 | SSU21 | ZRG16 |
PIGN |
Protein involved in GPI anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes; GPI stands for glycosylphosphatidylinositol |
| YKL172W |
EBP2 |
— |
EBNA1BP2 |
Protein required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering |
| YKL193C |
SDS22 |
EGP1 | type 1 protein phosphatase-activating protein SDS22 |
PPP1R7 |
Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function |
| YKL196C |
YKT6 |
palmitoyltransferase YKT6 |
YKT6 |
Vesicle membrane protein and R class v-SNARE; contains SNAP receptor and acyltransferase activities; subunit of multiple SNARE complexes involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and autophagosome-vacuolar fusion; C-terminal farnesylation and geranylgeranylation are essential for membrane localization and for function in cell wall integrity and autophagy; targeted to the vacuole via the AP-3 pathway; human homolog YKT6 complements a yeast ykt6 mutant |
| YKL203C |
TOR2 |
DRR2 | phosphatidylinositol kinase-related protein kinase TOR2 |
MTOR |
PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates homeostasis of plasma membrane (PM) tension and cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; PM retention is dependent on Avo3p; malonyl-CoA is a direct ATP-competitive inhibitor of TORC1; TOR2 has a paralog, TOR1, that arose from the whole genome duplication |
| YKL210W |
UBA1 |
E1 ubiquitin-activating protein UBA1 |
UBA7| UBA1 |
Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress |
| YKR008W |
RSC4 |
— |
— |
Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p |
| YKR022C |
NTR2 |
— |
— |
Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly |
| YKR037C |
SPC34 |
— |
— |
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body |
| YKR068C |
BET3 |
TRAPP complex core subunit BET3 |
TRAPPC3| TRAPPC3L |
Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); hydrophilic homodimeric protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles |
| YKR083C |
DAD2 |
HSK1 |
— |
Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis |
| YLL003W |
SFI1 |
— |
— |
Centrin (Cdc31p)-binding protein required for SPB duplication; localizes to the half-bridge of the spindle pole body (SPB); required for progression through G(2)-M transition; phosphorylated by Cdc28p-Clb2p and by Cdc5p; dephosphorylated by Cdc14p; has similarity to Xenopus laevis XCAP-C |
| YLL004W |
ORC3 |
origin recognition complex subunit 3 |
ORC3 |
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing |
| YLL011W |
SOF1 |
rRNA-processing protein SOF1 |
DCAF13 |
Protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p; essential gene |
| YLL050C |
COF1 |
cofilin |
CFL1| DSTN| CFL2 |
Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes |
| YLR002C |
NOC3 |
— |
NOC3L |
Subunit of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing |
| YLR005W |
SSL1 |
TFIIH/NER complex subunit SSL1 |
GTF2H2C| GTF2H2C_2| GTF2H2 |
Subunit of the core form of RNA polymerase transcription factor TFIIH; has both protein kinase and DNA-dependent ATPase/helicase activities; essential for transcription and nucleotide excision repair; interacts with Tfb4p |
| YLR008C |
PAM18 |
TIM14 |
DNAJC19| DNAJC15 |
Subunit of the import motor (PAM complex); the PAM complex is a component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p |
| YLR022C |
SDO1 |
guanine nucleotide exchange factor SDO1 |
SBDS |
Guanine nucleotide exchange factor (GEF) for Ria1p; essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes |
| YLR026C |
SED5 |
t-SNARE syntaxin |
STX5 |
cis-Golgi t-SNARE syntaxin; required for vesicular transport between the ER and the Golgi complex; binds at least 9 SNARE proteins; plays a role in autophagy by regulating formation of autophagic vesicles in Golgi |
| YLR045C |
STU2 |
— |
CKAP5 |
Microtubule polymerase and outer kinetochore component; required for anaphase spindle elongation, spindle orientation, and metaphase chromosome alignment; drives plus-end-directed kinetochore movement, enabling chromosome biorientation and congression; (de)stabilizes kinetochore–microtubule attachments in a tension-dependent manner; Cdc5p-mediated phosphorylation causes dissociation from the kinetochore Ndc80 complex; kinetochore function is essential; metazoan CKAP5/XMAP215 family member. |
| YLR060W |
FRS1 |
phenylalanine--tRNA ligase subunit beta |
FARSB |
Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control |
| YLR066W |
SPC3 |
signal peptidase complex subunit SPC3 |
SPCS3 |
Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23; other members of the complex are Spc1p, Spc2p, and Sec11p |
| YLR078C |
BOS1 |
SEC32 |
GOSR2 |
v-SNARE (vesicle specific SNAP receptor); localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi; required for efficient nuclear fusion during mating |
| YLR116W |
MSL5 |
BBP | mRNA splicing protein MSL5 |
SF1 |
Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia |
| YLR127C |
APC2 |
anaphase promoting complex subunit 2 | RSI1 | TID2 |
ANAPC2 |
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p |
| YLR163C |
MAS1 |
MIF1 | mitochondrial processing peptidase |
UQCRC1| PMPCB |
Beta subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins |
| YLR166C |
SEC10 |
exocyst subunit SEC10 |
EXOC5 |
Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion |
| YLR195C |
NMT1 |
CDC72 | glycylpeptide N-tetradecanoyltransferase NMT1 |
NMT2| NMT1 |
N-myristoyl transferase; catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction |
| YLR208W |
SEC13 |
ANU3 | GTPase-activating protein SEC13 |
SEC13 |
Structural component of 3 complexes; subunit of the Nup84p nuclear pore subcomplex that contributes to nucleocytoplasmic transport and NPC biogenesis; subunit of the COPII vesicle coat required for ER-to-Golgi transport; subunit of SEACAT, a subcomplex of the coatomer-related, vacuolar-associated SEA complex, that inhibits the TORC1 inhibitory role of SEACIT (Iml1p-Npr2p-Npr3p), a GAP for Gtr1p, thereby resulting in activation of TORC1 signaling; human SEC13 homolog |
| YLR212C |
TUB4 |
gamma-tubulin |
TUBG1| TUBG2 |
Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress; null mutation is complemented by human TUBG1 |
| YLR229C |
CDC42 |
Rho family GTPase CDC42 |
CDC42 |
Small rho-like GTPase; essential for establishment and maintenance of cell polarity; mediates a late stage of cell fusion during conjugation, focusing and anchoring Fus2p at the zone of cell fusion to facilitate the concentrated release of cell wall remodeling enzymes; acts with ESCRT proteins at sites of nuclear envelope and ER fission; mutants are defective in the organization of both actin and septins; human homolog CDC42 can complement yeast cdc42 mutants |
| YLR249W |
YEF3 |
eEF3 | EF-3 | TEF3 | translation elongation factor EF-3 |
— |
Translation elongation factor 3; contains two ABC cassettes; binds and hydrolyzes ATP; interacts with mRNA independently of translation; involved in frameshifting at stalled ribosomes; YEF3 has a paralog, HEF3, that arose from the whole genome duplication |
| YLR268W |
SEC22 |
SLY2 | SNAP receptor SEC22 | TS26 | TSL26 |
SEC22B |
R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog |
| YLR274W |
MCM5 |
BOB1 | CDC46 | MCM DNA helicase complex subunit MCM5 |
MCM5 |
Component of the Mcm2-7 hexameric helicase complex; MCM complex is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase |
| YLR293C |
GSP1 |
CNR1 | CST17 | Ran GTPase GSP1 |
RAN |
Ran GTPase; GTP binding protein (mammalian Ranp homolog) involved in the maintenance of nuclear organization, RNA processing and transport; regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p, Cse1p and Kap95p; GSP1 has a paralog, GSP2, that arose from the whole genome duplication |
| YLR310C |
CDC25 |
CDC25' | CTN1 | Ras family guanine nucleotide exchange factor CDC25 |
SOS1| RGL3| SOS2 |
Membrane bound guanine nucleotide exchange factor; also known as a GEF or GDP-release factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; thermosensitivity of the cdc25-5 mutant is functionally complemented by human RASGRF1 or by a fragment of human SOS1 comprising the CDC25-related catalytic domain |
| YLR314C |
CDC3 |
septin CDC3 |
SEPTIN5| SEPTIN2| SEPTIN1| SEPTIN4| SEPTIN7 |
Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells |
| YLR316C |
TAD3 |
— |
ADAT3 |
Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs; localizes to cytosol, nucleus and peroxisomes |
| YLR347C |
KAP95 |
karyopherin beta | RSL1 |
KPNB1 |
Karyopherin beta, forms importin complex with karyopherin alpha; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p |
| YLR378C |
SEC61 |
translocon subunit SEC61 |
SEC61A2| SEC61A1 |
Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex is required for SRP-independent protein translocation into the ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER |
| YLR430W |
SEN1 |
CIK3 | DNA/RNA helicase SEN1 | NRD2 |
SETX |
ATP-dependent 5' to 3' RNA/DNA and DNA helicase; subunit of the exosome-associated Nrd1p complex that mediates 3' end formation of snRNAs, snoRNAs, CUTs and some mRNAs; helicase-independent role in transcription-coupled repair; coordinates replication with transcription, associating with moving forks and preventing errors when forks encounter transcribed regions; S-phase-specific R-loop resolvase; homolog of Senataxin, implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of juvenile ALS |
| YLR440C |
SEC39 |
DSL3 |
— |
Component of the Dsl1p tethering complex; this complex interacts with ER SNAREs Sec20p and Use1p; mediates Sey1p-independent homotypic ER fusion; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope |
| YLR457C |
NBP1 |
— |
— |
Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication |
| YML015C |
TAF11 |
TAF40 | TafII40 | TATA-binding protein-associated factor TAF11 |
TAF11L9| TAF11L8| TAF11L10| TAF11L4| TAF11| TAF11L2| TAF11L13| TAF11L6| TAF11L14| TAF11L11| TAF11L7| TAF11L3| LINC02218| TAF11L5| TAF11L12 |
TFIID subunit (40 kDa); involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors |
| YML023C |
NSE5 |
Smc5-Smc6 complex subunit NSE5 |
— |
Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair |
| YML031W |
NDC1 |
— |
NDC1 |
Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 |
| YML046W |
PRP39 |
— |
PRPF39 |
U1 snRNP protein involved in splicing; contains multiple tetriatricopeptide repeats |
| YML065W |
ORC1 |
origin recognition complex subunit 1 |
ORC1 |
Largest subunit of the origin recognition complex; involved in directing DNA replication by binding to replication origins; also involved in transcriptional silencing; exhibits ATPase activity; role in chromatin organization at replication origins, along with chromatin remodellers; ORC1 has a paralog, SIR3, that arose from the whole genome duplication |
| YML069W |
POB3 |
FACT complex subunit POB3 |
SSRP1 |
Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress |
| YML077W |
BET5 |
TRAPP subunit BET5 |
TRAPPC1 |
Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); human homology TRAPPC1 complements yeast null mutant |
| YML098W |
TAF13 |
FUN81 | TAF19 | TafII19 |
TAF13 |
TFIID subunit (19 kDa); involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors |
| YML127W |
RSC9 |
— |
— |
Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway |
| YML130C |
ERO1 |
ER oxidoreductin |
ERO1B| ERO1A |
Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining ER redox balance; feedback regulated via reduction and oxidation of regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds; ero1-1 mutation complemented by human ERO1L |
| YMR013C |
SEC59 |
dolichol kinase |
DOLK |
Dolichol kinase; catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation |
| YMR033W |
ARP9 |
RSC12 | SWP59 |
— |
Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation |
| YMR061W |
RNA14 |
cleavage polyadenylation factor subunit RNA14 |
CSTF3 |
Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia |
| YMR076C |
PDS5 |
sister chromatid cohesion factor PDS5 |
PDS5B| PDS5A |
Cohesion maintenance factor; involved in sister chromatid condensation and cohesion; colocalizes with cohesin on chromosomes; performs its cohesin maintenance function in pre-anaphase cells by protecting the integrity of the cohesion complex; regulates homolog pairing and facilitates synaptonemal complex formation, axis formation, interhomolog recombination and synapsis during meiosis; relocalizes to the cytosol in response to hypoxia; scaffolding protein contains 26 HEAT repeats |
| YMR079W |
SEC14 |
phosphatidylinositol/phosphatidylcholine transfer protein SEC14 | PIT1 |
SEC14L4| SEC14L2| SEC14L6| SEC14L3| SEC14L5| SEC14L1 |
Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication |
| YMR094W |
CTF13 |
CBF3C |
— |
Subunit of the CBF3 complex; F-box protein of the leucine-rich-repeat family; required for assembly of the complex; CBF3 binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis |
| YMR117C |
SPC24 |
kinetochore-associated Ndc80 complex subunit SPC24 |
— |
Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p |
| YMR168C |
CEP3 |
CBF3 | CBF3B | CSL1 |
— |
Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain |
| YMR197C |
VTI1 |
v-SNARE protein VTI1 |
VTI1A |
Golgi membrane protein and Qb class v-SNARE; involved in cis-Golgi membrane traffic; interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways; human homolog VTI1A can complement the yeast null mutant |
| YMR213W |
CEF1 |
NTC85 |
CDC5L |
Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p |
| YMR235C |
RNA1 |
GTPase-activating protein RNA1 |
RANGAP1 |
GTPase activating protein (GAP) for Gsp1p; involved in nuclear transport |
| YMR268C |
PRP24 |
U6 snRNP complex subunit PRP24 |
— |
Splicing factor that reanneals snRNPs during spliceosome recycling; reanneals U4 and U6 snRNPs |
| YMR281W |
GPI12 |
N-acetylglucosaminylphosphatidylinositol deacetylase |
PIGL |
ER membrane protein involved in the second step of GPI anchor assembly; the second step is the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp; GPI stands for glycosylphosphatidylinositol |
| YMR308C |
PSE1 |
importin PSE1 | KAP121 |
IPO5| RANBP6 |
Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p; required for stability of the Dam1p-Duo1p complex |
| YNL002C |
RLP7 |
RPL7 |
— |
Nucleolar protein similar to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs; binds junction of ITS2 and ITS2-proximal stem between the 3' end of 5.8S rRNA and the 5' end of 25S rRNA |
| YNL006W |
LST8 |
TOR complex subunit LST8 |
MLST8 |
Subunit of TORC1 and TORC2 involved in TOR signaling; TORC1 regulates nutrient responsive growth and TORC2 regulates plasma membrane (PM) homeostasis and cell-cycle dependent actin cytoskeleton polarization; negative regulator of Rtg1p/3p and Gln3p transcription factors and amino acid biosynthesis; binds to both Tor1p and Tor2p, regulating the integrity and kinase activity of TORC2; PM retention is dependent on Avo3p; WD-repeat protein |
| YNL038W |
GPI15 |
phosphatidylinositol N-acetylglucosaminyltransferase GPI15 |
PIGH |
Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol |
| YNL102W |
POL1 |
CDC17 | CRT5 | DNA-directed DNA polymerase alpha catalytic subunit POL1 | HPR3 |
POLA1 |
Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis; H3-H4 histone chaperone, downstream of Pol32p, involved in the transfer of parental histones to the lagging strand during DNA replication |
| YNL118C |
DCP2 |
decapping enzyme complex catalytic subunit | PSU1 |
DCP2 |
Catalytic subunit of Dcp1p-Dcp2p decapping enzyme complex; removes 5' cap structure from mRNAs prior to their degradation; also enters nucleus and positively regulates transcription initiation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress; human homolog DCP2 complements yeast dcp2 thermosensitive mutant |
| YNL132W |
KRE33 |
ribosome biosynthesis protein KRE33 | RRA1 |
NAT10 |
Acetyltransferase required for biogenesis of small ribosomal subunit; conserved 18S rRNA cytosine acetyltransferase that modifies tRNAs assisted by adaptor Tan1p; responsible for incorporation of N4-acetylcytidine into mRNAs; human homolog NAT10 implicated in several types of cancer and premature aging |
| YNL158W |
PGA1 |
— |
— |
Essential component of GPI-mannosyltransferase II; complex is responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interactions with secretory pathway genes |
| YNL181W |
PBR1 |
putative oxidoreductase |
RDH14 |
Putative oxidoreductase; chaperone-like ER quality-control factor required for the folding and maturation of Fks1p; cytosol-facing ER membrane protein; member of a highly interconnected network of proteins including Fks1p, Ilm1p, and EMC complex components; required for cell viability |
| YNL207W |
RIO2 |
protein kinase RIO2 |
RIOK2 |
Essential serine kinase involved in the processing of 20S pre-rRNA; involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p |
| YNL216W |
RAP1 |
DNA-binding transcription factor RAP1 | GRC4 | GRF1 | TBA1 | TUF1 |
TERF2IP |
DNA-binding transcription regulator; involved in transcription activation, repression, chromatin silencing, telomere length maintenance; represses divergent noncoding transcription at highly expressed genes, prevents initiation at cryptic promoters; recruits Sir complex to telomeric DNA; forms telomere hyperclusters in quiescent cells; arrays of telomere-bound Rap1p stall condensin-induced DNA loop extrusion causing local chromatin decompaction in anaphase; relocalizes to cytosol under hypoxia |
| YNL232W |
CSL4 |
exosome non-catalytic core subunit CSL4 | SKI4 |
EXOSC1 |
Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; human homolog EXOSC1 partially complements yeast csl4 null mutant, and can complement inviability of strain in which expression of CSL4 is repressed |
| YNL258C |
DSL1 |
RNS1 |
— |
Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; mediates Sey1p-independent homotypic ER fusion; forms Dsl1 tethering complex with Sec39p and Tip20p that forms a stable complex with ER SNAREs Sec20p, Ufe1p and Use1p and is functionally conserved from yeast to mammalian cells; component of the ER target site that interacts with coatomer; interacts with different subunits of COPI vesicle coat; interacts with Cin5p; homolog of fly and human ZW10 gene |
| YNL261W |
ORC5 |
origin recognition complex subunit 5 |
ORC5 |
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing |
| YNL262W |
POL2 |
DNA polymerase epsilon catalytic subunit | DUN2 | Pol ε |
POLE |
Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p |
| YNL263C |
YIF1 |
protein transporter YIF1 |
YIF1B| YIF1A |
Integral membrane protein; required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3 |
| YNL272C |
SEC2 |
guanine nucleotide exchange factor SEC2 |
RAB3IP| RAB3IL1 |
Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles |
| YNR017W |
TIM23 |
MAS6 | MIM23 | MPI3 | protein transporter TIM23 |
TIMM23| TIMM23B |
Essential core component of the TIM23 complex; subunit of the translocase of the inner mitochondrial membrane involved in protein import into the mitochondrial matrix and inner membrane; provides a structural role, contributing to the architecture of the import channel; functions as a voltage sensor and pre-sequence receptor |
| YNR026C |
SEC12 |
Sar family guanine nucleotide exchange factor SEC12 | SED2 |
PREB |
Guanine nucleotide exchange factor (GEF); activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER; SEC12 has a paralog, SED4, that arose from the whole genome duplication |
| YNR035C |
ARC35 |
END9 |
ARPC2 |
Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin |
| YNR053C |
NOG2 |
NUG2 | putative GTPase NOG2 |
GNL2 |
Putative GTPase; associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2 |
| YOL038W |
PRE6 |
proteasome core particle subunit alpha 4 |
PSMA7| PSMA8 |
Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress |
| YOL094C |
RFC4 |
replication factor C subunit 4 |
RFC2 |
Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia |
| YOL123W |
HRP1 |
CFIB | NAB4 | NAB5 |
HNRNPA1| HNRNPD| HNRNPA3| MSI1| HNRNPAB| HNRNPDL |
Subunit of cleavage factor I and mRNA guard protein; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability; plays a role in copper homeostasis |
| YOL139C |
CDC33 |
eIF4E | TIF45 | translation initiation factor eIF4E |
EIF4E1B| EIF4E |
Translation initiation factor eIF4E; mRNA cap binding protein and subunit of the mRNA cap-binding complex (eIF4F) along with eIF4A and eIF4G, that mediates cap-dependent translation; required with eIF4G for Ded1p-mediated stimulation of mRNA recruitment during 48S preinitiation complex (PIC) assembly; connects protein synthesis to metabolic gene regulation; protein abundance increases in response to DNA replication stress; human homolog EIF4E can complement cdc33 mutants |
| YOR020C |
HSP10 |
CPN10 |
HSPE1 |
Mitochondrial matrix co-chaperonin; inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES |
| YOR075W |
UFE1 |
YOR29-26 |
STX18 |
t-SNARE protein required for retrograde vesicular traffic; involved in Sey1p-independent homotypic ER fusion; required for efficient nuclear fusion during mating; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER |
| YOR103C |
OST2 |
dolichyl-diphosphooligosaccharide-protein glycotransferase |
DAD1 |
Non-catalytic epsilon subunit of the oligosaccharyltransferase (OST) complex; the OST complex catalyzes asparagine-linked glycosylation of newly synthesized proteins in the ER; forms a pocket with Wbp1p involved in binding terminal glucose units of the donor glycan; located in the ER lumen |
| YOR122C |
PFY1 |
CLS5 | PRF1 | profilin |
PFN4 |
Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress; highly conserved protein; human PFN1 (profilin 1) complements temperature sensitive pfy1 mutants, PFN1 mutations are a rare cause of ALS |
| YOR143C |
THI80 |
thiamine diphosphokinase |
TPK1 |
Thiamine pyrophosphokinase; phosphorylates thiamine to produce the coenzyme thiamine pyrophosphate (thiamine diphosphate) |
| YOR151C |
RPB2 |
B150 | DNA-directed RNA polymerase II core subunit RPB2 | RPB150 | RPO22 | SIT2 | SOH2 |
POLR2B |
RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit |
| YOR174W |
MED4 |
— |
— |
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation |
| YOR181W |
LAS17 |
actin-binding protein LAS17 | BEE1 |
WAS| WASL |
Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments, polyproline domain able to nucleate actin filaments in absence of Arp2/3; contains 3 binding sites for monomeric actin which overlap with SH3 binding sites; binding between SH3 and actin is competitive; mutants are defective in endocytosis, bud site selection, cytokinesis; human homolog WAS (Wiskott-Aldrich Syndrome) implicated in severe immunodeficiency |
| YOR202W |
HIS3 |
HIS10 | HIS8 | imidazoleglycerol-phosphate dehydratase HIS3 |
— |
Imidazoleglycerol-phosphate dehydratase; catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p |
| YOR204W |
DED1 |
DEAD-box ATP-dependent RNA helicase DED1 | SPP81 |
DDX3Y| DDX3X |
ATP-dependent DEAD-box RNA helicase with strand-annealing activity; promotes eIF4F-dependent 48S translation preinitiation complex (PIC) assembly, stimulating recruitment of mRNAs with long, structured 5'-UTRs; cooperates with Dbp1p in PIC attachment and scanning; ATPase activity stimulated by mRNA cap-associated factor binding; directly binds eIF4G; role in spliceosomal complex disassembly; mutation in human homolog DBY associated with male infertility; human homolog DDX3X complements the null |
| YOR224C |
RPB8 |
ABC14.5 | DNA-directed RNA polymerase core subunit RPB8 |
POLR2H |
RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III |
| YOR232W |
MGE1 |
GRPE | mitochondrial nucleotide exchange factor MGE1 | YGE1 |
GRPEL1| GRPEL2 |
Mitochondrial matrix cochaperone; nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; acts as oxidative sensor to regulate mitochondrial Ssc1p; in presence of oxidative stress, dimeric Mge1p becomes a monomer and unable to regulate Ssc1p function; homolog of E. coli GrpE and human Mge1 (GRPEL1), which also responds to oxidative stress |
| YOR244W |
ESA1 |
KAT5 | NuA4 histone acetyltransferase complex catalytic subunit ESA1 | TAS1 |
KAT5 |
Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases, functionally complements lethality of the esa1 null mutation |
| YOR249C |
APC5 |
anaphase promoting complex subunit 5 | RMC1 |
ANAPC5 |
Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress |
| YOR254C |
SEC63 |
protein-transporting protein SEC63 | PTL1 |
SEC63 |
Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec62p, Sec66p, and Sec72p |
| YOR257W |
CDC31 |
centrin | DSK1 |
CETN3 |
Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation |
| YOR259C |
RPT4 |
CRL13 | PCS1 | proteasome regulatory particle base subunit RPT4 | SUG2 |
PSMC6 |
ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization |
| YOR260W |
GCD1 |
TRA3 | translation initiation factor eIF2B subunit gamma |
EIF2B3 |
Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression |
| YOR262W |
GPN2 |
GTPase GPN2 |
GPN2 |
Putative GTPase with a specific role in RNA pol II and polIII biogenesis; involved in the assembly and subsequent nuclear import of RNA polymerases II and III; required for establishment of sister chromatid cohesion; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B |
| YOR341W |
RPA190 |
A190 | DNA-directed RNA polymerase I core subunit RPA190 | RRN1 |
POLR1A |
RNA polymerase I largest subunit A190 |
| YOR362C |
PRE10 |
proteasome core particle subunit alpha 7 |
PSMA3 |
Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress |
| YOR370C |
MRS6 |
GTPase-activating protein MRS6 | MSI4 |
CHML| CHM |
Rab escort protein; type II geranylgeranyltransferase complex (Bet2p-Bet4p) chaperone that complexes with newly synthesized Rab GTPases, like Ypt1p and Sec4p, leading to their prenylation and membrane delivery; modulates the TOR pathway through interactions with Sfp1p; alters the kinetics of MAPK pathway activation and polarity reorganization during filamentous growth; sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress |
| YPL010W |
RET3 |
coatomer subunit zeta |
COPZ2| COPZ1 |
Zeta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER |
| YPL020C |
ULP1 |
NIB1 | SUMO protease ULP1 |
SENP3| SENP2| SENP1| SENP5 |
Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; contributes to regulation of telomeric silencing; localizes to the nuclear pore channel; sequestered to the nucleolus under stress conditions |
| YPL063W |
TIM50 |
protein translocase subunit TIM50 |
TIMM50 |
Essential component of the TIM23 complex; acts as receptor for the translocase of the inner mitochondrial membrane (TIM23) complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel |
| YPL076W |
GPI2 |
GCR4 | phosphatidylinositol N-acetylglucosaminyltransferase |
PIGC |
Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol |
| YPL082C |
MOT1 |
BTAF1 | BUR3 | DNA-binding ATPase | END10 | LPF4 |
BTAF1 |
DNA-binding ATPase and transcriptional regulator; forms a high affinity, TATA DNA binding complex with Spt15p (TBP) that has altered specificity, redistributing TBP from TATA-containing to TATA-less promoters; role in displacement of TBP from DNA that involves unbending, followed by ATP-driven, DNA translocation-mediated complex dissociation; coregulates transcription with Spt16p through PIC assembly and nucleosome organization; prevents R-loop accumulation and associated genomic instability |
| YPL094C |
SEC62 |
LPG14 | Sec63 complex subunit SEC62 |
SEC62 |
Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; cotranslationally N-acetylated by NatA; other members are Sec63p, Sec66p, and Sec72p |
| YPL128C |
TBF1 |
LPI16 |
— |
Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation; involved in nucleosome displacement during double-strand break repair |
| YPL160W |
CDC60 |
leucine--tRNA ligase CDC60 | LeuRS |
LARS1 |
Cytosolic leucyl tRNA synthetase; ligates leucine to the appropriate tRNA; human homolog LARS can complement yeast temperature-sensitive mutant at restrictive temperature |
| YPL169C |
MEX67 |
— |
NXF5| NXF2B| NXF2| NXF1| NXF3 |
Poly(A) RNA binding protein involved in nuclear mRNA export; component of the nuclear pore and nuclear RNA export factor; ubiquitin-associated (UBA) domain interacts with Mip6p; ortholog of human TAP |
| YPL209C |
IPL1 |
aurora kinase | PAC15 |
AURKB| AURKA| AURKC |
Aurora kinase of the chromosomal passenger complex; mediates release of mono-oriented kinetochores from microtubules in MI, and kinetochore release from SPB clusters during meiotic exit; maintains meiotic spindle checkpoint delay in a mek1 null; maintains condensed chromosomes in anaphase; required for SPB cohesion and preventing multipolar spindle formation; promotes telomerase release at G2/M; Iocalizes to nuclear foci that diffuse upon DNA replication stress; inhibits Pse1p upon SAC arrest |
| YPL252C |
YAH1 |
adrenodoxin |
FDX2 |
Ferredoxin of the mitochondrial matrix; required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; human homolog FDX1L can complement yeast by allowing growth during down-regulation of yeast YAH1 |
| YPR034W |
ARP7 |
RSC11 | SWP61 |
ACTL6A| ACTL6B |
Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation |
| YPR055W |
SEC8 |
exocyst subunit SEC8 |
EXOC4 |
Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress |
| YPR108W |
RPN7 |
proteasome regulatory particle lid subunit RPN7 |
PSMD6 |
Essential non-ATPase regulatory subunit of the 26S proteasome; similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits |
| YPR162C |
ORC4 |
origin recognition complex subunit 4 |
ORC4 |
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; ORC4 has a paralog, RIF2, that arose from the whole genome duplication |
| YPR178W |
PRP4 |
RNA4 | U4/U6-U5 snRNP complex subunit PRP4 |
PRPF4 |
Splicing factor; component of the U4/U6-U5 snRNP complex |
| YPR181C |
SEC23 |
GTPase-activating protein SEC23 |
SEC23A| SEC23B |
GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p |