CYCLoPs is a comprehensive Web database of protein abundance and localization changes for budding yeast Saccharomyces cerevisiae. CYCLoPs contains high resolution images covering about 75% of the yeast proteome (~4144 proteins) under multiple chemical and genetic perturbations. These images and quantitative measurements of changes in protein levels and sub-cellular localization following treatments were generated from high-content screening of yeast cells conducted in Moffat Lab and Andrews Lab at Terrence Donnelly Centre for Cellular and Biochemical Research (CCBR), University of Toronto.
The current version of CYCLoPs is 2.0-beta. Version 1.0 has retired on April 24, 2019. It was developed by Judice Koh, Yolanda Chong and Jason Moffat.
For details on the CYCLoPs project and data, contact Brenda Andrews.
For technical questions on the CYCLoPs website, contact Myra Paz Masinas.
The data stored in CYCLoPs correspond to studies described in Judice L.Y. Koh, Yolanda Chong, Brenda Andrews, Jason Moffat. CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces cerevisiae.
24 screens were conducted in the study, comprising of 2 chemical treatments and 1 genetic mutation. They include:
|AF100||alpha factor treatment||100 min||WT1|
|AF140||alpha factor treatment||140 min||WT1|
|AF180||alpha factor treatment||180 min||WT1|
|HU120||hydroxyurea treatment||120 min||WT3|
|HU160||hydroxyurea treatment||160 min||WT3|
|HU80||hydroxyurea treatment||80 min||WT3|
|RAP140||rapamycin treatment||140 min||WT3|
|RAP220||rapamycin treatment||220 min||WT3|
|RAP300||rapamycin treatment||300 min||WT3|
|RAP380||rapamycin treatment||380 min||WT3|
|RAP460||rapamycin treatment||460 min||WT3|
|RAP540||rapamycin treatment||540 min||WT3|
|RAP60||rapamycin treatment||60 min||WT3|
|RAP620||rapamycin treatment||620 min||WT3|
|RAP700||rapamycin treatment||700 min||WT3|