Cell. 2015 Jun 4;161(6):1413-24. doi: 10.1016/j.cell.2015.04.051. Mol Syst Biol. 2017 Apr; 13(4): 924. doi: 10.15252/msb.20177551
Collection of Yeast Cell and Localization Patterns

CYCLoPs is a comprehensive Web database of protein abundance and localization changes for budding yeast Saccharomyces cerevisiae. CYCLoPs contains high resolution images covering about 75% of the yeast proteome (~4144 proteins) under multiple chemical and genetic perturbations. These images and quantitative measurements of changes in protein levels and sub-cellular localization following treatments were generated from high-content screening of yeast cells conducted in Moffat Lab and Andrews Lab at Terrence Donnelly Centre for Cellular and Biochemical Research (CCBR), University of Toronto.

The current version of CYCLoPs is 2.0-beta. Version 1.0 has retired on April 24, 2019. It was developed by Judice Koh, Yolanda Chong and Jason Moffat.

For details on the CYCLoPs project and data, contact Brenda Andrews.

For technical questions on the CYCLoPs website, contact Myra Paz Masinas.

Database statistics
  • Proteins screened: 4,144
  • Total micrographs: 330,248
  • Total cells: > 2.7 million
  • Total screens: 24
  • Chong YT, Koh JLY, Friesen H, Duffy SK, Cox MJ, Moses A, Moffat J, Boone C, and Andrews BJ. Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis. Cell 2015 June 4; 161(6).
  • Koh JLY, Chong YT, Friesen H, Moses A, Boone C, Andrews BJ, Moffat J. CYCLoPs: a comprehensive database constructed from automated analysis of protein abundance and sub-cellular localization patterns in Saccharomyces cerevisiae. G3 2015 June 1; 5(6).

The data stored in CYCLoPs correspond to studies described in Judice L.Y. Koh, Yolanda Chong, Brenda Andrews, Jason Moffat. CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces cerevisiae.

24 screens were conducted in the study, comprising of 2 chemical treatments and 1 genetic mutation. They include:

Screen Condition Time course Wild-type
AF100 alpha factor treatment 100 min WT1
AF140 alpha factor treatment 140 min WT1
AF180 alpha factor treatment 180 min WT1
HU120 hydroxyurea treatment 120 min WT3
HU160 hydroxyurea treatment 160 min WT3
HU80 hydroxyurea treatment 80 min WT3
RAP140 rapamycin treatment 140 min WT3
RAP220 rapamycin treatment 220 min WT3
RAP300 rapamycin treatment 300 min WT3
RAP380 rapamycin treatment 380 min WT3
RAP460 rapamycin treatment 460 min WT3
RAP540 rapamycin treatment 540 min WT3
RAP60 rapamycin treatment 60 min WT3
RAP620 rapamycin treatment 620 min WT3
RAP700 rapamycin treatment 700 min WT3
rpd3Δ_1 rpd3 knockout WT3
rpd3Δ_2 rpd3 knockout WT3
rpd3Δ_3 rpd3 knockout WT3
WT1 wild-type
WT2 wild-type
WT3 wild-type