Standard name
Human Ortholog
Description Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0.08 – 0.07 0.06 0.05 0.06 0.06 0.1 0.16 0.21 0.13 0.19 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0.5 0.54 0.22 0.46 – 0.51 0.65 0.59 0.68 0.66 0 0 0 0 0.12 0.06 0 0 0 0 0 0
Nucleus 0.91 0.89 0.87 0.81 0.77 0.66 – 0.62 0.58 0.61 0.54 0.55 0.87 0.8 0.75 0.75 0.66 0.67 0.83 0.81 0.78 0.69 0.66 0.55
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0.11 0 0 0 0.05 – 0.08 0.07 0.07 0.07 0.05 0 0.05 0 0 0 0.05 0.07 0.05 0.1 0.14 0.16 0.17
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0.05 0.07 – 0 0 0 0.05 0.05 0 0 0 0.08 0.07 0.05 0 0 0.06 0.05 0.06 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 1
Bud 0 0 3 1 3 2 – 3 5 15 4 10 0 0 0 1 0 2 3 4 2 6 7 14
Bud Neck 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 1 1
Bud Site 0 0 1 0 5 6 – 8 15 16 14 41 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cytoplasm 4 4 15 14 22 39 – 50 28 27 46 48 41 87 126 24 17 26 0 0 3 3 0 10
Endoplasmic Reticulum 1 0 4 1 1 0 – 2 0 0 1 0 0 0 1 9 2 2 0 1 2 0 1 2
Endosome 1 1 0 1 14 4 – 2 0 4 1 2 2 2 10 1 3 0 8 3 3 3 9 9
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 1 0 2 3 0 1 2 1 1 1 2 2
Mitochondria 63 6 221 162 102 233 – 380 324 335 533 579 5 7 0 8 11 8 8 2 4 2 3 4
Nucleus 398 133 383 240 363 334 – 462 290 349 418 477 354 431 459 134 59 87 382 132 351 161 245 235
Nuclear Periphery 0 0 0 3 1 0 – 1 0 4 1 3 1 2 3 0 0 0 0 0 0 0 1 1
Nucleolus 21 17 19 12 16 25 – 61 35 40 55 45 15 26 12 6 2 7 34 8 47 33 58 71
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 5 0 5 1 3 1 – 4 0 4 0 6 2 2 4 1 3 2 8 1 2 6 14 19
Vac/Vac Membrane 7 5 3 2 22 34 – 18 20 17 38 41 4 12 19 14 6 7 8 4 25 12 22 47
Unique Cell Count 435 150 439 298 470 508 745 498 570 779 872 407 540 612 178 90 130 460 164 452 236 373 429
Labelled Cell Count 500 166 654 437 553 678 991 717 811 1111 1252 426 569 639 202 103 145 460 164 452 236 373 429


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.0 4.1 4.3 5.1 4.5 – 4.3 3.9 4.2 3.9 3.9 6.4 6.4 6.3 7.6 7.6 7.2 6.5 7.4 7.3
Std Deviation (1e-4) 0.8 1.2 0.9 1.2 1.1 1.4 – 1.2 1.3 1.1 1.0 1.0 1.3 1.4 1.4 1.8 2.0 1.9 1.2 1.5 1.4
Intensity Change (Log2) – – – 0.07 0.32 0.13 – 0.07 -0.07 0.04 -0.07 -0.08 0.65 0.64 0.64 0.9 0.9 0.83 0.67 0.86 0.85

WT3RAP60RAP140RAP220RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 1.0 2.8 – 2.4 1.6 1.0 1.9 1.7 3.9 6.5 8.1 4.7 5.6 6.4
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.6 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -9.0 0 – 0 0 0 0 0 -16.1 -18.1 -19.8 -10.7 -6.6 -9.0
Nucleus -2.5 -3.9 -7.7 – -9.3 -9.9 -9.2 -11.9 -11.7 -0.1 -3.1 -4.9 -3.7 -5.1 -5.4
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 -0.7 0 – 0 0 0 0 0 -0.5 0.4 -2.2 -0.5 0 0.5
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.7 4.8 – 2.2 3.3 2.6 3.9 3.8 0 1.9 2.7 0 0 0