Standard name
Human Ortholog
Description Pho85p cyclin of the Pho80p subfamily; forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated; PCL7 has a paralog, PCL6, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 1.0 0.96 0.93 0.92 0.93 0.87 0.8 0.81 0.76 0.81 0.95 0.99 1.0 0.95 0.95 0.94 0.98 0.96 0.93 0.87 0.86 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.08 0.08 0.18 0.16 0.38 0.24 0.34 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 1 0 1 2
Bud 0 1 0 0 4 1 4 2 1 7 10 5 0 0 0 0 0 0 0 0 3 4 5 12
Bud Neck 0 0 1 0 0 0 1 2 0 4 1 2 0 0 0 0 0 0 0 0 1 0 0 2
Bud Site 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 10 3 0 3 0 2 2 0 3 2 2 1 1 18 14 10 0 0 1 0 0 1
Cytoplasm 128 200 399 198 230 230 154 228 117 193 229 255 124 291 285 361 247 261 128 208 367 214 219 253
Endoplasmic Reticulum 1 1 0 0 2 0 1 0 0 0 2 0 0 0 0 14 12 10 0 0 0 4 4 2
Endosome 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 6 6 5 1 0 3 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 1 3
Mitochondria 1 6 0 7 19 21 30 41 56 57 103 86 3 2 0 14 4 3 0 4 2 2 4 7
Nucleus 1 1 0 3 5 1 3 2 1 2 1 1 0 0 0 0 2 1 0 0 0 3 2 1
Nuclear Periphery 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 2 0 0 1 1 0 1 2 1 4 2 0 0 0 0 0 0 0 7 3 9
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 2
SpindlePole 0 0 0 0 0 0 0 3 0 1 0 3 0 0 0 1 1 0 0 0 0 1 1 4
Vac/Vac Membrane 0 0 0 3 4 12 1 5 1 2 4 2 0 0 0 6 3 9 0 0 1 3 4 0
Unique Cell Count 129 207 400 206 246 250 166 261 146 237 303 316 130 293 285 379 259 278 132 217 394 246 256 311
Labelled Cell Count 131 211 411 216 264 268 195 289 180 267 357 359 134 296 286 422 290 303 132 217 394 246 256 311


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.4 6.2 5.7 5.0 4.9 3.7 4.9 4.1 4.2 4.0 4.1 8.0 6.7 5.4 6.6 8.4 8.2 9.6 9.0 11.4
Std Deviation (1e-4) 0.7 0.6 1.4 1.4 1.6 1.1 1.2 1.4 1.2 1.1 0.8 0.8 10.7 5.1 1.3 1.5 2.0 1.7 14.5 14.5 22.4
Intensity Change (Log2) – – – -0.12 -0.31 -0.36 -0.73 -0.34 -0.61 -0.58 -0.65 -0.59 0.37 0.1 -0.2 0.09 0.43 0.4 0.62 0.54 0.88

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000102030WT3HU80HU120HU1600102030WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30102030WT1AF100AF140AF1800102030
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –