Standard name | |
---|---|
Human Ortholog | |
Description | Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 4 | 16 | 5 | 1 | 0 | 0 | – | 1 | 0 | 1 | 1 | 5 | 2 | 0 | 13 | 12 | 4 | 2 | 2 | 2 | 2 | 8 | 3 |
Bud | 1 | 3 | 3 | 6 | 2 | 4 | 7 | – | 8 | 16 | 17 | 20 | 1 | 6 | 2 | 1 | 3 | 1 | 1 | 1 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 3 | – | 0 | 4 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 20 | 35 | 48 | 23 | 101 | 182 | 387 | – | 228 | 303 | 153 | 226 | 23 | 85 | 148 | 119 | 88 | 63 | 3 | 6 | 12 | 7 | 15 | 4 |
Endoplasmic Reticulum | 2 | 4 | 2 | 2 | 0 | 1 | 0 | – | 1 | 1 | 1 | 1 | 7 | 7 | 1 | 17 | 9 | 7 | 0 | 0 | 0 | 1 | 5 | 6 |
Endosome | 144 | 101 | 113 | 168 | 112 | 5 | 21 | – | 4 | 10 | 4 | 4 | 162 | 202 | 185 | 124 | 112 | 98 | 169 | 148 | 247 | 237 | 331 | 260 |
Golgi | 1 | 2 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 3 | 4 | 3 | 0 | 5 | 11 | 10 |
Mitochondria | 1 | 5 | 0 | 5 | 3 | 39 | 12 | – | 39 | 43 | 40 | 70 | 3 | 3 | 0 | 3 | 1 | 5 | 2 | 4 | 10 | 5 | 10 | 21 |
Nucleus | 0 | 0 | 0 | 1 | 2 | 2 | 3 | – | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 |
Nucleolus | 7 | 2 | 1 | 8 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 3 | 0 | 0 | 15 | 1 | 0 | 1 | – | 0 | 0 | 1 | 0 | 2 | 39 | 36 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 2 |
SpindlePole | 25 | 16 | 97 | 111 | 27 | 3 | 17 | – | 10 | 20 | 16 | 25 | 44 | 58 | 55 | 50 | 30 | 24 | 7 | 8 | 21 | 15 | 12 | 4 |
Vac/Vac Membrane | 50 | 42 | 41 | 15 | 145 | 17 | 41 | – | 3 | 10 | 7 | 7 | 20 | 23 | 28 | 90 | 70 | 50 | 0 | 5 | 13 | 6 | 14 | 22 |
Unique Cell Count | 178 | 164 | 245 | 247 | 302 | 226 | 441 | 267 | 366 | 210 | 307 | 217 | 309 | 349 | 305 | 247 | 197 | 194 | 184 | 314 | 290 | 425 | 349 | |
Labelled Cell Count | 257 | 215 | 321 | 361 | 395 | 255 | 493 | 298 | 408 | 246 | 361 | 270 | 426 | 458 | 422 | 325 | 256 | 194 | 184 | 314 | 290 | 425 | 349 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 7.2 | 6.6 | 6.1 | 6.3 | 3.9 | 4.8 | – | 4.0 | 4.1 | 3.8 | 4.0 | 6.1 | 6.2 | 6.2 | 8.0 | 8.5 | 7.8 | 6.6 | 6.3 | 6.6 |
Std Deviation (1e-4) | 1.3 | 1.1 | 1.1 | 1.2 | 1.6 | 1.0 | 1.5 | – | 1.2 | 1.5 | 1.0 | 1.3 | 1.2 | 1.4 | 1.7 | 1.1 | 1.0 | 1.1 | 1.7 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.06 | -0.77 | -0.45 | – | -0.73 | -0.68 | -0.81 | -0.71 | -0.13 | -0.1 | -0.1 | 0.27 | 0.35 | 0.23 | -0.01 | -0.07 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.5 | -4.2 | 0 | 0 | – | 0 | 0 | 0 | 0 | -2.2 | -3.9 | -4.8 | -1.2 | -0.8 | -2.3 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.2 | 3.6 | 13.2 | 17.8 | – | 14.9 | 15.5 | 11.4 | 12.6 | -2.7 | 2.2 | 5.8 | 4.9 | 4.0 | 3.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 2.1 | 0 |
Endosome | 4.9 | -2.1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 6.2 | 4.5 | 1.7 | -1.3 | -0.2 | 0.8 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 3.9 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 5.8 | 5.2 | 0 | 0 | 0 |
SpindlePole | 1.2 | -8.5 | 0 | 0 | – | 0 | 0 | 0 | 0 | -4.5 | -5.4 | -6.6 | -6.1 | -7.0 | -6.4 |
Vacuole | -3.7 | 7.7 | -3.0 | -2.9 | – | -6.3 | -6.1 | -4.6 | -6.0 | -2.4 | -3.4 | -3.3 | 3.5 | 3.1 | 2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9641 | 1.1512 | 0.9574 | 0.3125 | 1.2288 | 0.9299 | 1.9405 | 2.8125 | 2.9354 | 3.043 | 2.8468 | 2.9566 | 3.8174 | 5.329 | 4.6308 | 5.7314 | 3.6372 | 4.5675 |
Actin | 0.019 | 0.0043 | 0.0184 | 0 | 0.0579 | 0.0001 | 0.0044 | 0.0027 | 0.0061 | 0.0019 | 0.0198 | 0.0157 | 0.0039 | 0.0006 | 0.0023 | 0 | 0.0024 | 0.0004 |
Bud | 0.0025 | 0.0006 | 0.0004 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0023 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0835 | 0 |
Bud Neck | 0.0016 | 0.0003 | 0.0004 | 0.0001 | 0.0025 | 0.0003 | 0.0005 | 0.0007 | 0.0003 | 0.0005 | 0.0007 | 0.0006 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0006 | 0.0003 |
Bud Periphery | 0.0034 | 0.0001 | 0.0004 | 0 | 0.0009 | 0 | 0.0003 | 0 | 0.0004 | 0 | 0.0015 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0469 | 0 |
Bud Site | 0.0122 | 0.0036 | 0.0025 | 0.0002 | 0.0017 | 0.0001 | 0.0015 | 0.001 | 0.003 | 0.0002 | 0.0167 | 0.0002 | 0.0003 | 0.0005 | 0.001 | 0 | 0.0038 | 0.0001 |
Cell Periphery | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0012 | 0 |
Cytoplasm | 0.0175 | 0.0915 | 0.0671 | 0.1624 | 0.0078 | 0.1425 | 0.0599 | 0.1071 | 0.1295 | 0.0997 | 0.0121 | 0.0383 | 0.1137 | 0.1023 | 0.0569 | 0.0018 | 0.0217 | 0.0937 |
Cytoplasmic Foci | 0.2631 | 0.3325 | 0.3337 | 0.1146 | 0.2776 | 0.3273 | 0.3185 | 0.3293 | 0.3045 | 0.3012 | 0.2746 | 0.2576 | 0.333 | 0.2655 | 0.3426 | 0.7958 | 0.4176 | 0.3362 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0025 | 0.0057 | 0.0056 | 0.0029 | 0.0012 | 0.0066 | 0.003 | 0.0049 | 0.0044 | 0.0019 | 0.0027 | 0.001 | 0.0072 | 0.0043 | 0.0052 | 0 | 0.0006 | 0.0059 |
Endosome | 0.316 | 0.4553 | 0.4003 | 0.5441 | 0.3449 | 0.3539 | 0.3666 | 0.3787 | 0.3701 | 0.3669 | 0.4523 | 0.3886 | 0.3204 | 0.3482 | 0.318 | 0.069 | 0.3345 | 0.399 |
Golgi | 0.0563 | 0.023 | 0.0326 | 0.0734 | 0.1483 | 0.0378 | 0.0364 | 0.0332 | 0.0415 | 0.0127 | 0.1341 | 0.0444 | 0.0349 | 0.0465 | 0.0411 | 0.0108 | 0.0185 | 0.0372 |
Lipid Particles | 0.1371 | 0.0315 | 0.0587 | 0.0024 | 0.0735 | 0.077 | 0.0674 | 0.0255 | 0.0276 | 0.0096 | 0.0214 | 0.0419 | 0.0447 | 0.0523 | 0.0526 | 0.008 | 0.0131 | 0.0555 |
Mitochondria | 0.0452 | 0.0022 | 0.0022 | 0.0007 | 0.0386 | 0.0009 | 0.0048 | 0.0007 | 0.0029 | 0.0004 | 0.0176 | 0.0016 | 0.001 | 0.0008 | 0.0016 | 0.0001 | 0.0162 | 0.0008 |
None | 0.041 | 0.0188 | 0.0337 | 0.0043 | 0.0002 | 0.0161 | 0.0308 | 0.0237 | 0.0441 | 0.009 | 0.0014 | 0.0098 | 0.0406 | 0.1166 | 0.0691 | 0.0001 | 0.0118 | 0.0091 |
Nuclear Periphery | 0.0025 | 0.0026 | 0.002 | 0.0011 | 0.0005 | 0.001 | 0.0015 | 0.0023 | 0.0039 | 0.008 | 0.0015 | 0.0101 | 0.006 | 0.0009 | 0.0009 | 0 | 0.0008 | 0.0009 |
Nucleolus | 0.0006 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0004 | 0.0004 | 0.0001 | 0.0005 | 0 | 0.0021 | 0.0001 |
Nucleus | 0.0012 | 0.0006 | 0.0004 | 0.0007 | 0.0002 | 0.0006 | 0.0239 | 0.0355 | 0.0026 | 0.1408 | 0.0005 | 0.1074 | 0.0017 | 0.0003 | 0.0005 | 0 | 0.0048 | 0.0006 |
Peroxisomes | 0.0501 | 0.0131 | 0.0334 | 0.0018 | 0.0363 | 0.029 | 0.067 | 0.035 | 0.0398 | 0.0327 | 0.0291 | 0.0619 | 0.051 | 0.0442 | 0.0768 | 0.113 | 0.0115 | 0.0515 |
Punctate Nuclear | 0.0055 | 0.0014 | 0.0022 | 0.0003 | 0.001 | 0.0016 | 0.0038 | 0.0029 | 0.0027 | 0.0076 | 0.0017 | 0.0023 | 0.0371 | 0.0008 | 0.0098 | 0.0012 | 0.0016 | 0.0015 |
Vacuole | 0.019 | 0.01 | 0.0042 | 0.0719 | 0.0033 | 0.0039 | 0.0074 | 0.0146 | 0.0128 | 0.0047 | 0.007 | 0.0075 | 0.003 | 0.014 | 0.0185 | 0.0001 | 0.0052 | 0.0056 |
Vacuole Periphery | 0.0029 | 0.0026 | 0.0011 | 0.0189 | 0.0022 | 0.0012 | 0.0013 | 0.0017 | 0.0026 | 0.0018 | 0.0023 | 0.0098 | 0.0007 | 0.0019 | 0.0016 | 0 | 0.0017 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.1076 | 29.4441 | 26.704 | 34.7699 | 48.6361 | 29.2615 | 26.8765 | 31.2563 | 19.5204 | 34.8278 |
Translational Efficiency | 0.9517 | 1.0568 | 1.0574 | 0.5738 | 0.5603 | 0.6002 | 0.5757 | 0.5047 | 0.6402 | 0.5938 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1775 | 463 | 1559 | 1279 | 1322 | 1938 | 2489 | 1071 | 3097 | 2401 | 4048 | 2350 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 802.36 | 856.60 | 988.84 | 904.89 | 726.72 | 892.01 | 969.20 | 893.33 | 770.07 | 885.18 | 976.76 | 899.62 |
Standard Deviation | 95.74 | 189.80 | 117.12 | 124.09 | 93.88 | 114.85 | 117.20 | 132.01 | 102.05 | 133.37 | 117.56 | 127.89 |
Intensity Change Log 2 | — | 0.094372 | 0.301487 | 0.173493 | — | 0.295660 | 0.415395 | 0.297794 | — | 0.193545 | 0.356746 | 0.233906 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000334 | 0.001595 | 0.003344 | 0.002919 | 0.000330 | 0.000318 | 0.002761 | 0.001406 | 0.000332 | 0.000564 | 0.002985 | 0.002230 |
Bud Neck | 0.004110 | 0.006805 | 0.017793 | 0.005584 | 0.005750 | 0.005490 | 0.004008 | 0.012256 | 0.004810 | 0.005743 | 0.009317 | 0.008625 |
Bud Site | 0.003065 | 0.014397 | 0.027212 | 0.017925 | 0.002466 | 0.003329 | 0.013565 | 0.024332 | 0.002810 | 0.005463 | 0.018821 | 0.020845 |
Cell Periphery | 0.000023 | 0.000073 | 0.000072 | 0.000040 | 0.000027 | 0.000054 | 0.000028 | 0.000130 | 0.000025 | 0.000058 | 0.000045 | 0.000081 |
Cytoplasm | 0.007064 | 0.019222 | 0.029777 | 0.010606 | 0.010669 | 0.007293 | 0.009550 | 0.029877 | 0.008603 | 0.009593 | 0.017340 | 0.019388 |
Cytoplasmic Foci | 0.165758 | 0.250509* | 0.189804* | 0.226787* | 0.226057* | 0.158031 | 0.292354* | 0.177432 | 0.191498* | 0.175864 | 0.252859* | 0.204294* |
Eisosomes | 0.000024 | 0.000050 | 0.000039 | 0.000020 | 0.000044 | 0.000028 | 0.000017 | 0.000029 | 0.000032 | 0.000032 | 0.000025 | 0.000024 |
Endoplasmic Reticulum | 0.000125 | 0.000349 | 0.002675 | 0.000589 | 0.000193 | 0.000412 | 0.000428 | 0.000858 | 0.000154 | 0.000399 | 0.001293 | 0.000712 |
Endosome | 0.684678* | 0.520771* | 0.262498* | 0.478143* | 0.567676* | 0.665002* | 0.442462* | 0.456303* | 0.634734* | 0.637189* | 0.373153* | 0.468189* |
Golgi | 0.034584 | 0.047984 | 0.008263 | 0.022192 | 0.064105 | 0.047298 | 0.024045 | 0.017005 | 0.047185 | 0.047430 | 0.017967 | 0.019828 |
Lipid Particles | 0.015048 | 0.019138 | 0.021529 | 0.012104 | 0.029664 | 0.022797 | 0.020267 | 0.011678 | 0.021287 | 0.022091 | 0.020753 | 0.011910 |
Mitochondria | 0.005257 | 0.002632 | 0.002716 | 0.002086 | 0.006216 | 0.005022 | 0.001143 | 0.005319 | 0.005667 | 0.004561 | 0.001749 | 0.003560 |
Mitotic Spindle | 0.006549 | 0.010483 | 0.209515* | 0.067388 | 0.004078 | 0.006947 | 0.051579 | 0.073843 | 0.005494 | 0.007629 | 0.112405* | 0.070330 |
None | 0.000072 | 0.000411 | 0.000436 | 0.000165 | 0.000342 | 0.000490 | 0.000014 | 0.002159 | 0.000187 | 0.000475 | 0.000177 | 0.001074 |
Nuclear Periphery | 0.000079 | 0.000125 | 0.003267 | 0.000518 | 0.000062 | 0.000137 | 0.000187 | 0.000901 | 0.000072 | 0.000135 | 0.001374 | 0.000693 |
Nuclear Periphery Foci | 0.001549 | 0.001835 | 0.021571 | 0.005488 | 0.001260 | 0.002110 | 0.003833 | 0.006031 | 0.001425 | 0.002057 | 0.010664 | 0.005735 |
Nucleolus | 0.000423 | 0.002079 | 0.004315 | 0.000504 | 0.000567 | 0.001735 | 0.000138 | 0.002705 | 0.000485 | 0.001801 | 0.001747 | 0.001507 |
Nucleus | 0.001097 | 0.005325 | 0.013065 | 0.000667 | 0.000823 | 0.001571 | 0.000166 | 0.004673 | 0.000980 | 0.002295 | 0.005134 | 0.002493 |
Peroxisomes | 0.049326 | 0.072921 | 0.053469 | 0.080662 | 0.068398 | 0.046932 | 0.102574* | 0.070652 | 0.057467 | 0.051943 | 0.083662 | 0.076100 |
Vacuole | 0.019516 | 0.021547 | 0.127098 | 0.062168 | 0.010204 | 0.022765 | 0.030243 | 0.094788 | 0.015541 | 0.022530 | 0.067545 | 0.077035 |
Vacuole Periphery | 0.001318 | 0.001750 | 0.001543 | 0.003445 | 0.001067 | 0.002241 | 0.000637 | 0.007622 | 0.001211 | 0.002146 | 0.000986 | 0.005349 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.53 | -4.74 | -3.06 | -1.26 | 0.34 | 0.13 | -6.75 | -2.47 | -2.51 | 2.88 | -1.85 | -8.11 | -3.79 | -3.26 | 1.58 |
Bud Neck | -1.72 | -15.49 | -3.33 | 0.02 | 13.45 | 0.20 | 1.02 | -5.04 | -5.87 | -6.95 | -1.26 | -10.39 | -6.13 | -4.81 | 3.12 |
Bud Site | -4.43 | -14.65 | -8.38 | -1.53 | 3.90 | -1.88 | -13.39 | -10.21 | -9.80 | -4.14 | -4.20 | -19.41 | -13.09 | -10.69 | -0.77 |
Cell Periphery | -3.12 | -11.45 | -4.02 | 2.01 | 7.33 | -2.60 | -1.32 | -2.06 | -1.39 | -1.97 | -3.71 | -9.44 | -2.56 | -0.80 | -1.22 |
Cytoplasm | -3.18 | -13.33 | -4.59 | 0.84 | 8.90 | 1.81 | -1.03 | -7.44 | -8.74 | -7.23 | -0.78 | -10.71 | -8.76 | -7.79 | -0.81 |
Cytoplasmic Foci | -9.28 | 1.64 | -8.29 | 3.65 | -9.41 | 11.10 | -9.26 | 9.15 | -1.04 | 20.51 | 3.51 | -10.42 | -0.04 | -3.38 | 9.80 |
Eisosomes | -2.99 | -7.62 | 0.79 | 3.36 | 12.52 | 3.71 | 6.87 | 2.25 | -2.21 | -7.80 | -0.02 | 1.36 | 2.01 | 1.94 | 1.26 |
Endoplasmic Reticulum | -2.73 | -12.94 | -2.61 | -1.33 | 9.44 | -2.03 | -1.14 | -3.80 | -2.50 | -1.48 | -2.98 | -8.48 | -4.49 | -2.37 | 4.37 |
Endosome | 11.23 | 56.57* | 23.30 | 4.63 | -24.14* | -9.36 | 14.28 | 13.40 | 23.46 | 2.41 | -0.28 | 43.47* | 26.16 | 24.96 | -10.98 |
Golgi | -3.19 | 17.77 | 7.12 | 6.81 | -9.55 | 4.94 | 14.37 | 16.70 | 13.98 | 5.67 | -0.08 | 19.35 | 17.08 | 14.83 | -0.97 |
Lipid Particles | -1.25 | -3.69 | 1.61 | 2.20 | 6.54 | 2.34 | 3.34 | 7.21 | 5.59 | 6.20 | -0.43 | 0.05 | 6.47 | 6.13 | 8.72 |
Mitochondria | 2.41 | 2.49 | 3.22 | 0.80 | 1.65 | 1.02 | 5.11 | 1.29 | 0.48 | -3.52 | 1.28 | 5.54 | 3.08 | 2.03 | -2.61 |
Mitotic Spindle | -1.52 | -26.47* | -13.17 | -11.45 | 13.56 | -2.23 | -16.91 | -13.38 | -12.86 | -4.18 | -1.83 | -29.67 | -18.73 | -18.01 | 5.78 |
None | -1.80 | -3.76 | -1.00 | 1.27 | 2.58 | -0.61 | 2.09 | -1.81 | -1.62 | -2.21 | -1.58 | -0.08 | -1.93 | -1.16 | -1.94 |
Nuclear Periphery | -1.04 | -13.11 | -7.39 | -6.37 | 11.45 | -2.50 | -8.35 | -4.87 | -4.54 | -4.00 | -2.42 | -13.41 | -7.26 | -6.66 | 6.65 |
Nuclear Periphery Foci | -0.11 | -13.92 | -4.00 | -4.13 | 9.86 | -1.69 | -4.94 | -5.85 | -4.76 | -2.89 | -0.89 | -13.50 | -6.62 | -6.02 | 5.16 |
Nucleolus | -1.94 | -8.02 | -1.95 | 1.41 | 7.28 | -2.74 | 2.76 | -5.30 | -2.34 | -6.26 | -3.49 | -6.93 | -5.61 | -0.31 | 1.43 |
Nucleus | -2.20 | -11.26 | -0.74 | 2.02 | 11.10 | -1.82 | 1.49 | -4.98 | -4.11 | -5.26 | -2.50 | -10.33 | -4.63 | -1.87 | 5.75 |
Peroxisomes | -3.48 | 1.81 | -5.59 | -0.45 | -7.18 | 4.81 | -6.27 | 1.10 | -3.37 | 7.25 | 1.76 | -6.32 | -3.45 | -4.95 | 2.20 |
Vacuole | -0.79 | -28.86 | -13.47 | -11.24 | 12.65 | -6.38 | -13.27 | -18.87 | -16.85 | -13.90 | -3.97 | -27.94 | -22.80 | -20.54 | -3.91 |
Vacuole Periphery | -1.67 | -0.76 | -3.21 | -2.32 | -3.06 | -4.83 | 2.87 | -4.16 | -3.22 | -4.52 | -4.96 | 2.77 | -5.18 | -3.68 | -5.62 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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