Standard name | |
---|---|
Human Ortholog | |
Description | N-glycosylated protein; involved in the maintenance of bud site selection during bipolar budding; localization requires Rax1p; RAX2 mRNA stability is regulated by Mpt5p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 141 | 63 | 172 | 129 | 164 | 189 | 245 | 244 | 229 | 206 | 144 | 118 | 123 | 159 | 179 | 187 | 135 | 177 | 100 | 33 | 57 | 43 | 86 | 75 |
Bud | 61 | 17 | 69 | 46 | 90 | 96 | 99 | 148 | 119 | 107 | 74 | 74 | 43 | 70 | 95 | 77 | 52 | 79 | 33 | 9 | 21 | 21 | 32 | 28 |
Bud Neck | 1 | 0 | 2 | 1 | 6 | 3 | 2 | 3 | 7 | 4 | 4 | 4 | 0 | 0 | 1 | 2 | 6 | 1 | 87 | 46 | 81 | 43 | 58 | 54 |
Bud Site | 57 | 25 | 58 | 72 | 50 | 77 | 95 | 76 | 102 | 88 | 62 | 67 | 75 | 79 | 84 | 45 | 13 | 41 | – | – | – | – | – | – |
Cell Periphery | 47 | 16 | 33 | 18 | 19 | 21 | 40 | 36 | 41 | 43 | 30 | 32 | 12 | 33 | 39 | 43 | 47 | 63 | 75 | 18 | 34 | 9 | 23 | 21 |
Cytoplasm | 17 | 11 | 22 | 24 | 38 | 46 | 78 | 81 | 60 | 53 | 51 | 50 | 13 | 20 | 27 | 94 | 40 | 44 | 3 | 1 | 1 | 0 | 1 | 2 |
Endoplasmic Reticulum | 18 | 8 | 6 | 6 | 5 | 6 | 13 | 12 | 5 | 9 | 4 | 3 | 5 | 11 | 15 | 15 | 11 | 16 | 67 | 14 | 24 | 19 | 34 | 51 |
Endosome | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 9 | 4 | 0 | 2 | 3 | 3 | 1 | 2 | 0 | 3 | 3 |
Golgi | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 16 | 10 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 10 | 18 | 26 | 15 | 31 | 52 | 122 | 135 | 157 | 97 | 142 | 22 | 18 | 41 | 34 | 18 | 30 | 3 | 12 | 11 | 0 | 2 | 3 |
Nucleus | 17 | 0 | 2 | 8 | 1 | 6 | 12 | 6 | 10 | 4 | 6 | 9 | 4 | 6 | 12 | 22 | 9 | 11 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 |
Nucleolus | 1 | 0 | 0 | 3 | 0 | 0 | 3 | 5 | 2 | 1 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 9 | 7 | 16 | 10 | 5 | 23 | 45 | 26 | 44 | 32 | 28 | 38 | 22 | 43 | 57 | 3 | 10 | 22 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 8 | 0 | 8 | 27 | 4 | 10 | 20 | 12 | 20 | 20 | 12 | 6 | 6 | 14 | 20 | 32 | 16 | 28 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 4 | 5 | 7 | 27 | 14 | 26 | 15 | 17 | 33 |
Unique Cell Count | 268 | 102 | 229 | 209 | 238 | 286 | 394 | 471 | 421 | 413 | 283 | 308 | 192 | 271 | 309 | 381 | 270 | 366 | 410 | 159 | 264 | 158 | 263 | 278 |
Labelled Cell Count | 387 | 159 | 406 | 370 | 399 | 509 | 705 | 775 | 775 | 727 | 514 | 548 | 325 | 463 | 577 | 569 | 380 | 535 | 410 | 159 | 264 | 158 | 263 | 278 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.0 | 10.0 | 7.6 | 6.9 | 7.0 | 6.6 | 6.2 | 5.8 | 5.6 | 5.3 | 5.2 | 4.9 | 8.7 | 8.1 | 6.9 | 11.3 | 13.8 | 13.7 | 11.0 | 10.1 | 10.3 |
Std Deviation (1e-4) | 2.4 | 2.2 | 1.4 | 1.2 | 1.2 | 1.3 | 1.3 | 1.1 | 1.4 | 1.2 | 1.2 | 0.9 | 1.5 | 1.3 | 1.1 | 2.3 | 2.8 | 2.7 | 2.0 | 1.9 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.13 | -0.2 | -0.28 | -0.39 | -0.44 | -0.51 | -0.54 | -0.64 | 0.19 | 0.09 | -0.14 | 0.57 | 0.86 | 0.85 | 0.53 | 0.4 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.0 | -1.5 | -2.2 | -3.3 | -5.9 | -5.2 | -6.2 | -5.6 | -8.5 | -2.5 | -3.9 | -4.1 | -6.3 | -5.7 | -6.4 |
Bud | -1.9 | 1.8 | 0.8 | -1.4 | 0.3 | -0.5 | -1.1 | -1.0 | -1.6 | -1.8 | -1.1 | 0.2 | -2.8 | -2.8 | -2.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2.1 | -1.1 | 0.4 | -0.3 | -2.9 | -0.3 | -1.2 | -0.9 | -1.0 | 3.0 | 1.0 | 0.5 | -4.3 | -6.5 | -4.5 |
Cell Periphery | -1.9 | -2.2 | -2.6 | -1.6 | -2.8 | -1.8 | -1.5 | -1.3 | -1.4 | -2.7 | -0.7 | -0.6 | -1.1 | 0.9 | 0.9 |
Cytoplasm | 0.6 | 2.1 | 2.2 | 3.3 | 2.7 | 1.7 | 1.2 | 2.7 | 2.2 | -1.0 | -0.9 | -0.3 | 4.6 | 1.8 | 0.9 |
Endoplasmic Reticulum | 0.2 | -0.4 | -0.4 | 0.5 | -0.1 | 0 | -0.4 | 0 | 0 | 0 | 0.9 | 1.3 | 0.9 | 0.9 | 1.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 |
Mitochondria | 1.6 | -0.7 | 1.1 | 2.0 | 5.6 | 6.9 | 8.2 | 7.1 | 9.6 | 1.3 | -0.5 | 2.0 | 0.5 | -0.5 | 0.1 |
Nucleus | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 3.0 | 1.9 | 1.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -1.0 | -2.5 | 0.4 | 1.8 | -0.8 | 1.5 | 0.4 | 1.2 | 2.0 | 1.6 | 3.1 | 3.8 | -4.3 | -1.6 | -0.5 |
SpindlePole | 3.6 | -1.2 | 0 | 0.9 | -0.7 | 0.8 | 0.8 | 0.4 | -1.1 | -0.2 | 0.9 | 1.5 | 2.4 | 1.3 | 2.1 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.4019 | 10.307 | 9.3645 | 8.8891 | 9.5292 | 9.9045 | 7.8305 | 11.0317 | 10.1176 | 10.7077 | 10.0438 | 11.5222 | 6.9696 | 9.4382 | 8.1129 | 8.0872 | 8.8638 | 9.2197 |
Actin | 0.0885 | 0.0568 | 0.1306 | 0.0899 | 0.1714 | 0.0826 | 0.0759 | 0.0481 | 0.0824 | 0.0235 | 0.1124 | 0.0421 | 0.1271 | 0.105 | 0.1023 | 0.1048 | 0.1731 | 0.0674 |
Bud | 0.0063 | 0.0046 | 0.0159 | 0.0082 | 0.0085 | 0.0042 | 0.0065 | 0.0059 | 0.0154 | 0.0102 | 0.0124 | 0.0043 | 0.0048 | 0.0095 | 0.0193 | 0.0096 | 0.0052 | 0.0038 |
Bud Neck | 0.2275 | 0.0241 | 0.2485 | 0.408 | 0.4637 | 0.7903 | 0.1387 | 0.0464 | 0.1865 | 0.2688 | 0.1609 | 0.6532 | 0.2099 | 0.0719 | 0.2432 | 0.3397 | 0.2821 | 0.6952 |
Bud Periphery | 0.021 | 0.0079 | 0.0566 | 0.0721 | 0.0482 | 0.0224 | 0.0203 | 0.0151 | 0.061 | 0.0293 | 0.0625 | 0.0243 | 0.0135 | 0.0182 | 0.0784 | 0.0992 | 0.0427 | 0.0264 |
Bud Site | 0.4574 | 0.7693 | 0.4172 | 0.1136 | 0.0896 | 0.0171 | 0.2009 | 0.5414 | 0.1808 | 0.0245 | 0.046 | 0.0141 | 0.3592 | 0.6055 | 0.2854 | 0.0296 | 0.0656 | 0.0216 |
Cell Periphery | 0.119 | 0.0359 | 0.0623 | 0.1087 | 0.0627 | 0.0315 | 0.0963 | 0.0788 | 0.1084 | 0.0447 | 0.1074 | 0.0471 | 0.1147 | 0.0493 | 0.0967 | 0.1587 | 0.1273 | 0.0606 |
Cytoplasm | 0.033 | 0.065 | 0.0305 | 0.0892 | 0.0357 | 0.0093 | 0.0442 | 0.0499 | 0.0418 | 0.0467 | 0.0968 | 0.0151 | 0.0251 | 0.0631 | 0.0554 | 0.0566 | 0.0342 | 0.0166 |
Cytoplasmic Foci | 0.0118 | 0.0034 | 0.0052 | 0.012 | 0.0056 | 0.006 | 0.0803 | 0.0068 | 0.018 | 0.026 | 0.0262 | 0.0063 | 0.0167 | 0.0103 | 0.0127 | 0.0077 | 0.0138 | 0.0077 |
Eisosomes | 0.0018 | 0.0005 | 0.0015 | 0.0058 | 0.0019 | 0.0017 | 0.0013 | 0.0031 | 0.0012 | 0.0002 | 0.0015 | 0.0006 | 0.0026 | 0.001 | 0.001 | 0.0031 | 0.0051 | 0.0014 |
Endoplasmic Reticulum | 0.0016 | 0.0033 | 0.0036 | 0.0025 | 0.058 | 0.0023 | 0.0114 | 0.0195 | 0.02 | 0.0252 | 0.0293 | 0.0268 | 0.0076 | 0.0048 | 0.0079 | 0.0105 | 0.0217 | 0.012 |
Endosome | 0.0047 | 0.0034 | 0.0048 | 0.0167 | 0.0085 | 0.0058 | 0.0799 | 0.018 | 0.0569 | 0.1255 | 0.0618 | 0.0293 | 0.0107 | 0.0076 | 0.0267 | 0.0377 | 0.0415 | 0.012 |
Golgi | 0.0022 | 0.0052 | 0.0071 | 0.0336 | 0.0154 | 0.0118 | 0.032 | 0.0067 | 0.0318 | 0.0642 | 0.0538 | 0.0277 | 0.0144 | 0.0169 | 0.0176 | 0.0188 | 0.0735 | 0.0284 |
Lipid Particles | 0.0063 | 0.0021 | 0.003 | 0.011 | 0.0145 | 0.004 | 0.0379 | 0.0183 | 0.0158 | 0.0318 | 0.0363 | 0.0136 | 0.0143 | 0.0082 | 0.0142 | 0.0457 | 0.0141 | 0.0108 |
Mitochondria | 0.0019 | 0.0005 | 0.0015 | 0.0019 | 0.0012 | 0.0008 | 0.0155 | 0.0011 | 0.0163 | 0.0054 | 0.0062 | 0.0027 | 0.0128 | 0.0058 | 0.0017 | 0.0016 | 0.004 | 0.004 |
None | 0.0012 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0081 | 0.0007 | 0.0006 | 0.0003 | 0.0004 | 0.0004 | 0.0017 | 0.0003 | 0.0005 | 0.0212 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.0005 | 0.0003 | 0.0004 | 0.0004 | 0.001 | 0.0002 | 0.016 | 0.0024 | 0.0023 | 0.0043 | 0.0029 | 0.0022 | 0.0017 | 0.0007 | 0.0012 | 0.0009 | 0.0013 | 0.0006 |
Nucleolus | 0.001 | 0.0044 | 0.0009 | 0.002 | 0.0007 | 0.0005 | 0.0034 | 0.002 | 0.0025 | 0.0028 | 0.0011 | 0.0011 | 0.0014 | 0.0007 | 0.0015 | 0.0018 | 0.0011 | 0.0008 |
Nucleus | 0.001 | 0.0023 | 0.0011 | 0.0021 | 0.0011 | 0.0006 | 0.0205 | 0.0031 | 0.0033 | 0.0035 | 0.0022 | 0.0021 | 0.0024 | 0.0011 | 0.0018 | 0.0019 | 0.0013 | 0.0012 |
Peroxisomes | 0.0036 | 0.0003 | 0.0009 | 0.0011 | 0.0007 | 0.0013 | 0.0177 | 0.0005 | 0.001 | 0.0013 | 0.0022 | 0.0005 | 0.0092 | 0.001 | 0.0017 | 0.0006 | 0.0018 | 0.0007 |
Punctate Nuclear | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.018 | 0.0005 | 0.0006 | 0.0009 | 0.0005 | 0.0004 | 0.0012 | 0.0004 | 0.0004 | 0.0005 | 0.0003 | 0.0002 |
Vacuole | 0.0088 | 0.0101 | 0.0074 | 0.0198 | 0.0101 | 0.0067 | 0.0675 | 0.1293 | 0.148 | 0.2466 | 0.1707 | 0.0818 | 0.0478 | 0.0179 | 0.0293 | 0.0477 | 0.0848 | 0.0262 |
Vacuole Periphery | 0.0002 | 0.0002 | 0.0004 | 0.0009 | 0.0007 | 0.0004 | 0.0077 | 0.0023 | 0.0056 | 0.0142 | 0.0065 | 0.0044 | 0.0011 | 0.0005 | 0.0012 | 0.002 | 0.0053 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.1527 | 25.851 | 31.7711 | 43.2193 | 20.309 | 19.5691 | 39.411 | 45.2978 | 30.5515 | 35.3128 |
Translational Efficiency | 0.6565 | 0.7365 | 0.7554 | 0.4358 | 0.7519 | 0.8983 | 0.5531 | 0.4975 | 0.6021 | 0.5004 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2034 | 28 | 211 | 1510 | 510 | 2099 | 530 | 264 | 2544 | 2127 | 741 | 1774 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 871.72 | 322.34 | 1290.88 | 1288.13 | 836.10 | 1058.29 | 1195.62 | 1363.80 | 864.58 | 1048.60 | 1222.75 | 1299.39 |
Standard Deviation | 102.08 | 5.15 | 161.65 | 199.09 | 159.37 | 152.84 | 137.10 | 179.26 | 116.73 | 173.47 | 150.77 | 198.10 |
Intensity Change Log 2 | — | -1.435282 | 0.566418 | 0.563341 | — | 0.339988 | 0.516012 | 0.705885 | — | -0.306829 | 0.541961 | 0.634887 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.013150 | 0.000033 | 0.083854 | 0.042633 | 0.043291 | 0.028424 | 0.043360 | 0.026687 | 0.019192 | 0.028050 | 0.054890 | 0.040260 |
Bud Neck | 0.359567* | 0.000008 | 0.225140* | 0.149892 | 0.236177* | 0.289440* | 0.225126* | 0.211413* | 0.334831* | 0.285630* | 0.225130* | 0.159048 |
Bud Site | 0.174595* | 0.000016 | 0.473495* | 0.391688* | 0.426405* | 0.318229* | 0.528107* | 0.320793* | 0.225076* | 0.314040* | 0.512557* | 0.381137* |
Cell Periphery | 0.402953* | 0.000049 | 0.118654* | 0.335061* | 0.206907* | 0.290396* | 0.116048* | 0.381751* | 0.363652* | 0.286574* | 0.116790* | 0.342009* |
Cytoplasm | 0.025284 | 0.000027 | 0.024770 | 0.027231 | 0.026668 | 0.028420 | 0.033794 | 0.013114 | 0.025561 | 0.028046 | 0.031224 | 0.025130 |
Cytoplasmic Foci | 0.002226 | 0.000014 | 0.006622 | 0.003231 | 0.007871 | 0.003591 | 0.007992 | 0.000492 | 0.003358 | 0.003544 | 0.007602 | 0.002824 |
Eisosomes | 0.005915 | 0.000043 | 0.012349 | 0.012488 | 0.003953 | 0.004832 | 0.007239 | 0.014959 | 0.005521 | 0.004769 | 0.008694 | 0.012856 |
Endoplasmic Reticulum | 0.004586 | 0.000001 | 0.015094 | 0.005241 | 0.002633 | 0.011512 | 0.005083 | 0.002955 | 0.004194 | 0.011361 | 0.007933 | 0.004901 |
Endosome | 0.001231 | 0.000001 | 0.003213 | 0.004451 | 0.011582 | 0.004020 | 0.005768 | 0.002045 | 0.003306 | 0.003967 | 0.005040 | 0.004093 |
Golgi | 0.000901 | 0.000005 | 0.002834 | 0.003495 | 0.005000 | 0.002256 | 0.001644 | 0.000655 | 0.001723 | 0.002226 | 0.001983 | 0.003072 |
Lipid Particles | 0.000861 | 0.000031 | 0.003944 | 0.005898 | 0.002157 | 0.002495 | 0.003050 | 0.002800 | 0.001121 | 0.002463 | 0.003305 | 0.005437 |
Mitochondria | 0.002876 | 0.000010 | 0.000263 | 0.002351 | 0.014988 | 0.003645 | 0.000410 | 0.000634 | 0.005304 | 0.003597 | 0.000368 | 0.002096 |
Mitotic Spindle | 0.000206 | 0.000316 | 0.000273 | 0.000767 | 0.000383 | 0.000261 | 0.000585 | 0.001070 | 0.000242 | 0.000261 | 0.000496 | 0.000812 |
None | 0.000211 | 0.999298* | 0.000017 | 0.000037 | 0.000190 | 0.000038 | 0.000020 | 0.000015 | 0.000207 | 0.013192 | 0.000019 | 0.000034 |
Nuclear Periphery | 0.000028 | 0.000000 | 0.000032 | 0.000073 | 0.000294 | 0.000041 | 0.000058 | 0.000056 | 0.000081 | 0.000040 | 0.000051 | 0.000070 |
Nuclear Periphery Foci | 0.000156 | 0.000025 | 0.001665 | 0.000233 | 0.000221 | 0.000412 | 0.001607 | 0.000294 | 0.000169 | 0.000407 | 0.001624 | 0.000242 |
Nucleolus | 0.000031 | 0.000027 | 0.000010 | 0.000022 | 0.000438 | 0.000063 | 0.000017 | 0.000014 | 0.000113 | 0.000062 | 0.000015 | 0.000021 |
Nucleus | 0.001207 | 0.000011 | 0.002340 | 0.000454 | 0.001040 | 0.000587 | 0.001460 | 0.000303 | 0.001173 | 0.000580 | 0.001711 | 0.000432 |
Peroxisomes | 0.001632 | 0.000084 | 0.000787 | 0.001354 | 0.004592 | 0.003393 | 0.001336 | 0.000394 | 0.002225 | 0.003349 | 0.001179 | 0.001211 |
Vacuole | 0.002205 | 0.000001 | 0.024519 | 0.011885 | 0.004219 | 0.006746 | 0.016949 | 0.018070 | 0.002609 | 0.006657 | 0.019105 | 0.012805 |
Vacuole Periphery | 0.000181 | 0.000000 | 0.000124 | 0.001514 | 0.000990 | 0.001199 | 0.000348 | 0.001487 | 0.000343 | 0.001183 | 0.000284 | 0.001510 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 12.42 | -7.02 | -11.14 | -17.53 | 4.03 | 3.06 | -0.01 | 2.73 | 0.41 | 2.98 | -4.44 | -8.41 | -8.44 | -4.63 | 3.20 |
Bud Neck | 56.47* | 7.59 | 26.37 | -31.28 | 4.18 | -4.49 | 0.78 | 1.50 | 5.54 | 0.79 | 6.10 | 10.48 | 24.50 | 17.19 | 6.50 |
Bud Site | 40.23* | -14.63 | -22.86 | -46.37* | 3.70 | 7.60 | -5.29 | 4.71 | -0.14 | 8.92 | -11.63 | -22.79 | -17.23 | -6.87 | 9.34 |
Cell Periphery | 59.56* | 22.53 | 6.35 | -39.70* | -15.91 | -6.60 | 6.59 | -8.05 | -4.62 | -12.97 | 8.89 | 27.39 | 2.27 | -5.54 | -22.04 |
Cytoplasm | 12.78 | -0.13 | -0.73 | -12.84 | -0.26 | -0.43 | -1.36 | 3.10 | 4.85 | 4.13 | -0.98 | -1.65 | 0.11 | 1.06 | 1.70 |
Cytoplasmic Foci | 4.74 | -2.71 | -1.58 | -6.20 | 2.06 | 2.46 | -0.11 | 4.40 | 4.87 | 4.54 | -0.21 | -3.24 | 0.64 | 0.82 | 3.59 |
Eisosomes | 17.08 | -2.66 | -8.26 | -17.38 | -0.08 | -1.26 | -3.15 | -5.77 | -5.56 | -3.90 | 1.81 | -3.32 | -10.01 | -11.15 | -3.74 |
Endoplasmic Reticulum | 6.66 | -2.77 | -0.72 | -8.80 | 2.61 | -8.04 | -2.63 | -0.39 | 6.87 | 1.95 | -6.22 | -2.79 | -0.93 | 5.69 | 2.30 |
Endosome | 6.90 | -2.55 | -4.46 | -6.31 | -1.18 | 3.95 | 2.46 | 4.60 | 2.03 | 2.22 | -1.16 | -1.56 | -1.13 | -0.26 | 0.75 |
Golgi | 4.76 | -2.94 | -2.99 | -4.10 | -0.65 | 2.71 | 3.60 | 4.75 | 2.79 | 2.36 | -0.91 | -0.70 | -1.79 | -0.98 | -1.41 |
Lipid Particles | 4.31 | -3.61 | -4.39 | -5.16 | -1.31 | -0.27 | -0.68 | -0.38 | -0.24 | 0.20 | -2.69 | -3.32 | -4.26 | -2.82 | -1.83 |
Mitochondria | 6.29 | 5.68 | 0.83 | -5.29 | -4.68 | 3.68 | 4.78 | 4.69 | 6.47 | -0.99 | 2.06 | 6.85 | 3.95 | 2.69 | -4.50 |
Mitotic Spindle | -1.94 | -1.52 | -1.98 | -1.59 | -1.71 | 1.37 | -0.95 | -0.76 | -0.89 | -0.41 | -0.32 | -1.47 | -2.08 | -1.93 | -0.77 |
None | -7600.55* | 2.80 | 2.52 | 9093.89* | -3.92 | 1.87 | 2.09 | 2.15 | 6.22 | 1.03 | -5.25 | 3.24 | 2.99 | 5.33 | -3.21 |
Nuclear Periphery | 9.36 | -1.03 | -4.43 | -7.49 | -3.78 | 0.94 | 0.86 | 0.88 | -1.56 | 0.11 | 0.76 | 0.49 | 0.20 | -3.43 | -0.72 |
Nuclear Periphery Foci | 7.33 | -4.48 | -1.85 | -4.21 | 4.13 | -1.05 | -1.24 | -1.42 | 0.66 | 1.17 | -1.35 | -1.82 | -1.97 | 0.76 | 1.69 |
Nucleolus | 0.61 | 6.45 | 2.57 | 0.69 | -4.43 | 1.10 | 1.23 | 1.24 | 2.61 | 0.92 | 0.72 | 1.41 | 1.33 | 2.24 | -1.70 |
Nucleus | 4.30 | -1.99 | 2.72 | -15.78 | 3.48 | 1.98 | -1.26 | 3.23 | 5.46 | 4.30 | 2.63 | -1.72 | 3.26 | 2.98 | 5.18 |
Peroxisomes | 6.30 | 3.05 | 0.78 | -5.16 | -2.04 | 0.96 | 2.71 | 3.54 | 5.78 | 3.01 | -1.95 | 2.87 | 2.72 | 4.03 | -0.11 |
Vacuole | 9.71 | -4.99 | -8.25 | -10.24 | 2.84 | -2.26 | -4.81 | -3.85 | -3.13 | -0.16 | -4.80 | -7.39 | -8.98 | -4.59 | 2.64 |
Vacuole Periphery | 6.80 | 1.06 | -3.02 | -3.43 | -3.14 | -0.54 | 2.02 | -0.44 | -0.26 | -1.02 | -2.84 | 0.39 | -2.83 | -0.66 | -2.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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