Standard name
Human Ortholog
Description Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0.07 0.1 0.08 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.25 0 0 0 0.12 0.16 0.38 0.33 0.45 0.17 0.08 0 0 0.07 0 0.11 0 0 0 0 0 0
Nucleus 0.91 0.89 0.92 0.93 0.95 0.9 0.87 0.82 0.83 0.82 0.9 0.91 0.91 0.88 0.81 0.87 0.76 0.8 0.84 0.84 0.87 0.83 0.78
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.09 0 0 0 0 0 0.07 0.06 0.07 0 0.13 0.05 0.06 0.05 0.05 0.06 0.11 0 0.05 0.07 0.07 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 1
Bud 0 0 0 0 0 0 1 1 4 3 2 0 0 0 1 0 0 3 5 3 0 3 4
Bud Neck 0 0 0 0 2 0 2 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 1 7 6 9 14 6 0 1 0 1 0 0
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10 9 13 16 22 11 21 15 12 2 10 15 37 51 25 13 15 1 1 0 0 0 0
Endoplasmic Reticulum 0 0 1 0 1 0 0 1 0 0 0 2 0 1 7 5 9 0 0 1 0 1 2
Endosome 0 1 0 1 1 2 0 0 0 0 0 1 2 0 6 0 3 4 7 0 1 2 4
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 3 0 1 2
Mitochondria 9 77 4 12 4 35 97 143 165 159 80 32 18 14 21 5 22 1 3 1 0 3 3
Nucleus 184 274 240 307 500 253 525 311 416 290 429 358 469 436 258 201 149 169 278 222 236 327 338
Nuclear Periphery 1 1 2 3 2 0 5 2 2 1 3 2 1 2 1 1 0 0 0 0 0 0 0
Nucleolus 33 28 5 4 3 3 22 26 30 25 20 50 27 31 16 12 12 23 10 12 19 27 35
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
SpindlePole 1 1 3 3 8 2 6 5 15 2 18 3 6 1 6 1 6 0 3 1 0 4 2
Vac/Vac Membrane 1 7 4 1 3 2 1 4 5 2 4 1 2 3 12 7 8 2 17 15 8 15 30
Unique Cell Count 203 309 260 329 529 280 602 378 501 353 479 393 516 497 317 232 195 212 331 266 272 396 432
Labelled Cell Count 239 398 274 347 547 309 687 514 658 499 574 464 564 539 356 245 226 212 331 266 272 396 432


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 5.5 6.7 6.3 6.7 5.2 5.1 4.7 4.6 4.3 5.1 6.1 6.4 6.3 8.9 9.7 8.4 5.9 6.0 6.3
Std Deviation (1e-4) 1.1 1.2 1.4 1.4 1.5 1.3 1.2 1.2 1.1 0.9 1.0 1.2 1.5 1.5 2.6 2.7 3.1 1.1 1.3 1.6
Intensity Change (Log2) -0.1 -0.0 -0.36 -0.39 -0.52 -0.56 -0.66 -0.4 -0.15 -0.08 -0.11 0.4 0.52 0.32 -0.19 -0.17 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -0.5 -0.6 -1.0 -0.6 -1.9 -3.5 -2.2 -0.7 1.2 2.5 1.4 0.3 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.6 0 4.9 6.1 0 0 0 6.2 3.6 1.5 1.1 3.0 0 4.4
Nucleus 0.5 1.2 -0.8 -2.2 -3.6 -3.5 -3.6 -1.2 -0.5 -0.7 -1.9 -3.8 -2.1 -4.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1.3 0 0 0 1.6 4.9 2.2 2.6 2.0 2.0 2.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2.0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 1.6 1.1 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9054 4.1043 3.5248 3.6047 3.9167 4.196 1.5499 2.2859 2.1924 1.6227 1.7881 1.9037 1.9862 2.5146 1.8569 2.4737 1.8871 1.9867
Actin 0.0452 0 0.004 0 0.1471 0.0157 0.0188 0 0.0056 0 0.0633 0.0086 0.0449 0.0001 0.0089 0.0016 0 0.0058
Bud 0.0006 0 0.0002 0 0.0008 0.0003 0.0008 0 0.0004 0 0.0002 0.0001 0.0013 0.0001 0.0002 0.0002 0 0.0001
Bud Neck 0.0095 0.0001 0.0007 0.0001 0.0078 0.0022 0.0045 0 0.0003 0 0.0023 0.001 0.0009 0.0001 0.0003 0.0005 0.0004 0.0008
Bud Periphery 0.0012 0 0.0003 0 0.0014 0.0007 0.0018 0 0.0006 0 0.0003 0.0002 0.0023 0.0001 0.0002 0.0007 0 0.0006
Bud Site 0.0148 0.0001 0.0127 0 0.0069 0.002 0.0145 0 0.0024 0 0.0015 0.0001 0.0025 0.0001 0.0007 0.0004 0.0001 0.0002
Cell Periphery 0.0007 0 0.0006 0 0.0007 0.0001 0.0003 0 0.0001 0 0.0002 0 0.0003 0 0.0001 0.0003 0 0.0001
Cytoplasm 0.0012 0 0.0041 0.0002 0.0041 0.0002 0.0014 0.0001 0.0011 0 0.0007 0.0001 0.0054 0.0001 0.002 0.0002 0 0.0002
Cytoplasmic Foci 0.0193 0 0.0021 0 0.122 0.0012 0.017 0 0.002 0 0.0432 0.0005 0.0333 0.0002 0.0112 0.0002 0 0.0008
Eisosomes 0.0005 0 0.0001 0 0.0062 0.0002 0.001 0 0.0001 0 0.0003 0 0.0004 0 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0043 0 0.0008 0 0.0018 0.0006 0.0036 0 0.0005 0 0.0003 0.0003 0.0065 0.0001 0.0012 0.0014 0 0.0014
Endosome 0.0158 0 0.0029 0 0.0513 0.0033 0.0159 0 0.0067 0 0.0472 0.0046 0.0303 0.0034 0.0097 0.0021 0 0.0047
Golgi 0.0079 0 0.0019 0 0.0375 0.0038 0.0068 0 0.0024 0 0.0289 0.0011 0.0152 0.0055 0.0105 0.0006 0 0.0052
Lipid Particles 0.0245 0 0.003 0 0.0926 0.0039 0.0159 0 0.0061 0 0.0487 0.0004 0.031 0.0001 0.0117 0.0031 0 0.0066
Mitochondria 0.0158 0.0001 0.0017 0.0002 0.0222 0.0041 0.0451 0.0002 0.0165 0.0001 0.0046 0.0044 0.024 0.0019 0.0062 0.0059 0.0002 0.0148
None 0.0011 0 0.0056 0.0006 0.0123 0.0002 0.0098 0 0.0004 0 0.0003 0 0.0064 0 0.0017 0.0004 0 0.0002
Nuclear Periphery 0.0111 0.0003 0.0023 0.0015 0.002 0.0009 0.0345 0.0015 0.0077 0.0002 0.0021 0.0008 0.0101 0.0004 0.0026 0.0299 0.0004 0.0102
Nucleolus 0.0344 0.0188 0.0194 0.02 0.0483 0.1049 0.0232 0.0192 0.0236 0.0089 0.0942 0.0536 0.0153 0.0233 0.0084 0.0219 0.0976 0.0424
Nucleus 0.7611 0.9803 0.9201 0.9726 0.3689 0.8512 0.7512 0.9768 0.9164 0.9907 0.6341 0.92 0.7255 0.964 0.9135 0.9223 0.8976 0.8999
Peroxisomes 0.0212 0 0.0052 0 0.0445 0.0013 0.0079 0 0.0019 0 0.0165 0.002 0.0144 0 0.0059 0.0002 0 0.0008
Punctate Nuclear 0.0042 0.0002 0.0117 0.0046 0.019 0.0026 0.0108 0.0017 0.0026 0.0001 0.0076 0.0003 0.0234 0.0003 0.0036 0.0032 0.0036 0.0031
Vacuole 0.0033 0 0.0004 0.0001 0.0013 0.0004 0.0034 0.0001 0.0017 0 0.0029 0.0007 0.0038 0.0001 0.0008 0.002 0.0001 0.0008
Vacuole Periphery 0.0024 0 0.0001 0 0.0013 0.0002 0.0117 0.0002 0.0011 0 0.0007 0.001 0.0025 0.0002 0.0006 0.0028 0 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.8932 14.9103 17.0217 19.0578 16.0805 16.2427 23.0003 19.5676 18.794 23.1239
Translational Efficiency 0.4877 0.657 0.5708 0.4888 0.4988 0.4837 0.5176 0.5221 0.5568 0.4468

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1789 297 1083 641 1918 850 869 5 3707 1147 1952 646

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 762.86 1006.26 1040.00 965.79 840.74 890.50 1000.75 955.89 803.16 920.47 1022.53 965.71
Standard Deviation 100.39 141.39 143.04 141.94 108.30 129.30 132.62 298.50 111.57 141.90 139.87 143.81
Intensity Change Log 2 0.399513 0.447093 0.340291 0.082956 0.251350 0.185185 0.242223 0.347785 0.261054

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000303 0.001115 0.001177 0.003826 0.000269 0.000722 0.001131 0.004996 0.000285 0.000823 0.001157 0.003835
Bud Neck 0.003849 0.024817 0.028956 0.046260 0.004533 0.010998 0.019888 0.004066 0.004203 0.014576 0.024919 0.045933
Bud Site 0.001886 0.002857 0.002236 0.049703 0.000455 0.001952 0.002009 0.003617 0.001146 0.002186 0.002135 0.049346
Cell Periphery 0.000253 0.000127 0.000057 0.000393 0.000067 0.000126 0.000059 0.000436 0.000157 0.000126 0.000058 0.000394
Cytoplasm 0.018575 0.001578 0.002886 0.021867 0.001365 0.001460 0.001224 0.004014 0.009670 0.001491 0.002146 0.021728
Cytoplasmic Foci 0.002052 0.002391 0.000063 0.015346 0.000274 0.001898 0.000688 0.019832 0.001132 0.002026 0.000341 0.015381
Eisosomes 0.000022 0.000038 0.000048 0.000057 0.000021 0.000037 0.000044 0.000036 0.000022 0.000037 0.000047 0.000057
Endoplasmic Reticulum 0.002566 0.006917 0.003215 0.006651 0.003071 0.006666 0.004284 0.001873 0.002827 0.006731 0.003691 0.006614
Endosome 0.000617 0.001004 0.000169 0.030415 0.000204 0.001699 0.000465 0.012973 0.000403 0.001519 0.000301 0.030280
Golgi 0.000172 0.002292 0.000167 0.029137 0.000166 0.000885 0.000477 0.182820* 0.000169 0.001249 0.000305 0.030327
Lipid Particles 0.000405 0.000325 0.000083 0.002740 0.000127 0.002236 0.000130 0.001752 0.000261 0.001741 0.000104 0.002733
Mitochondria 0.001152 0.002217 0.001609 0.017659 0.000665 0.007679 0.001517 0.002364 0.000900 0.006265 0.001568 0.017540
Mitotic Spindle 0.000986 0.012900 0.002752 0.089629 0.000553 0.007129 0.001890 0.129588* 0.000762 0.008623 0.002368 0.089938
None 0.018403 0.005121 0.001851 0.006572 0.002797 0.002307 0.002787 0.025628 0.010329 0.003036 0.002268 0.006719
Nuclear Periphery 0.000322 0.001041 0.000268 0.003498 0.000489 0.001150 0.000321 0.000458 0.000408 0.001122 0.000291 0.003475
Nuclear Periphery Foci 0.001049 0.000431 0.000189 0.003203 0.000386 0.001644 0.000139 0.000979 0.000706 0.001330 0.000167 0.003185
Nucleolus 0.031143 0.028476 0.042939 0.011124 0.036153 0.044254 0.022720 0.016494 0.033735 0.040168 0.033938 0.011165
Nucleus 0.913094* 0.902900* 0.909255* 0.625504* 0.947333* 0.900386* 0.937981* 0.582381* 0.930809* 0.901037* 0.922043* 0.625171*
Peroxisomes 0.000803 0.000973 0.000846 0.003261 0.000521 0.001747 0.000494 0.001011 0.000657 0.001547 0.000690 0.003244
Vacuole 0.001946 0.001823 0.001028 0.022682 0.000344 0.003453 0.001571 0.002837 0.001117 0.003031 0.001270 0.022528
Vacuole Periphery 0.000403 0.000657 0.000205 0.010472 0.000209 0.001573 0.000180 0.001844 0.000303 0.001336 0.000194 0.010406

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.21 -18.20 -7.92 -4.97 -4.53 NaN NaN NaN NaN NaN -9.05 -25.80 -8.08 -6.23 -4.64
Bud Neck -7.45 -19.72 -13.27 -3.50 -2.32 NaN NaN NaN NaN NaN -9.34 -22.59 -13.17 -8.44 -3.95
Bud Site -1.55 -2.09 -5.90 -5.51 -5.59 NaN NaN NaN NaN NaN -2.83 -5.35 -6.20 -5.83 -5.68
Cell Periphery 1.69 3.46 0.26 -2.07 -8.34 NaN NaN NaN NaN NaN 0.75 3.45 -2.42 -3.33 -8.55
Cytoplasm 8.69 7.77 1.52 -5.71 -5.13 NaN NaN NaN NaN NaN 8.03 7.41 -1.92 -5.79 -5.67
Cytoplasmic Foci 0.21 3.97 -1.38 -1.06 -4.13 NaN NaN NaN NaN NaN -0.94 3.49 -2.78 -1.90 -4.05
Eisosomes -5.42 -15.41 -11.93 -5.71 -3.75 NaN NaN NaN NaN NaN -7.91 -21.76 -12.41 -6.98 -4.10
Endoplasmic Reticulum -8.09 -3.86 -12.12 -1.77 -9.69 NaN NaN NaN NaN NaN -9.42 -7.50 -11.84 -2.65 -8.78
Endosome -0.85 3.03 -4.50 -4.26 -4.78 NaN NaN NaN NaN NaN -3.24 1.53 -4.70 -4.01 -4.79
Golgi -1.09 -0.97 -3.24 -1.97 -3.22 NaN NaN NaN NaN NaN -2.05 -1.08 -3.34 -2.90 -3.30
Lipid Particles 0.66 3.63 -2.35 -2.60 -3.59 NaN NaN NaN NaN NaN -1.80 3.25 -3.00 0.66 -3.56
Mitochondria -2.50 -2.86 -4.79 -3.88 -4.28 NaN NaN NaN NaN NaN -3.55 -6.30 -5.06 -0.63 -4.31
Mitotic Spindle -2.12 -2.03 -8.13 -5.04 -7.68 NaN NaN NaN NaN NaN -3.95 -2.74 -8.22 -6.75 -7.89
None 5.21 9.57 6.94 -0.51 -6.47 NaN NaN NaN NaN NaN 7.07 9.30 4.12 -3.65 -6.26
Nuclear Periphery -1.65 -0.62 -8.18 -3.05 -8.13 NaN NaN NaN NaN NaN -3.29 0.11 -7.60 -4.06 -8.02
Nuclear Periphery Foci 2.01 1.88 -0.61 -2.74 -2.55 NaN NaN NaN NaN NaN -1.01 2.30 -1.67 -0.18 -3.16
Nucleolus 1.12 -6.75 7.40 3.86 11.71 NaN NaN NaN NaN NaN -2.59 -2.95 9.48 9.64 10.26
Nucleus 0.86 3.33 11.13 8.62 9.68 NaN NaN NaN NaN NaN 5.30 5.79 13.30 10.03 11.54
Peroxisomes -0.27 -0.48 -0.85 -0.82 -0.79 NaN NaN NaN NaN NaN -2.20 -0.59 -1.64 0.88 -1.66
Vacuole -0.30 1.22 -7.10 -7.00 -7.43 NaN NaN NaN NaN NaN -3.27 -1.83 -7.49 -6.65 -7.30
Vacuole Periphery -0.75 0.94 -3.56 -3.16 -3.89 NaN NaN NaN NaN NaN -2.75 1.02 -3.79 -2.10 -3.94