Standard name
Description Minor isoform of pyruvate decarboxylase; key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.81 0.81 0.88 0.89 0.88 0.87 0.86 0.81 0.79 0.70 0.82 0.77 0.90 0.90 0.43 0.54 0.58 0.65 0.68 0.62 0.57 0.79 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.10 0.09 0.28 0.19 0.41 0.22 0.06 0 0 0 0 0 0.07 0 0.07 0 0 0
Nucleus 0.06 0.09 0.07 0.08 0.05 0 0 0 0 0 0 0.09 0.10 0.09 0.17 0.17 0.09 0.12 0.14 0.11 0.22 0.05 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.15 0.05 0 0.09 0 0 0 0 0 0 0.11 0 0.05 0.49 0.39 0.43 0.06 0.08 0.10 0.06 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 1 3 0 0 0
Bud 0 0 0 0 0 2 4 6 2 7 6 0 0 0 0 0 0 6 4 1 0 0 0
Bud Neck 0 1 2 5 7 4 12 7 9 4 14 0 0 0 0 0 0 3 1 2 0 0 0
Bud Site 0 0 0 0 0 1 0 1 1 4 1 0 0 0 0 0 0
Cell Periphery 0 1 0 2 0 1 4 0 3 3 2 0 0 0 2 3 1 0 0 2 0 0 0
Cytoplasm 201 180 170 304 280 347 470 263 262 354 478 27 27 95 62 90 118 169 166 125 13 35 41
Endoplasmic Reticulum 1 0 0 0 1 0 0 0 3 4 0 0 0 0 5 4 3 1 0 0 0 0 0
Endosome 0 1 2 2 1 3 5 0 0 0 1 0 0 1 4 5 8 2 2 1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Mitochondria 20 2 1 5 1 40 48 90 64 206 126 2 0 0 4 1 1 17 7 14 0 0 1
Nucleus 15 19 14 26 17 16 14 6 7 9 3 3 3 9 24 29 19 30 35 22 5 2 3
Nuclear Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 1 1 1 5 12 8 6 8 15 0 0 0 0 2 0 6 1 1 0 1 0
Vac/Vac Membrane 29 34 9 9 28 16 12 7 10 5 12 4 0 5 70 64 88 16 18 20 1 3 4
Unique Cell Count 248 223 194 342 319 399 544 325 331 504 585 35 30 105 144 166 205 262 244 201 24 45 56
Labelled Cell Count 267 240 199 354 336 436 582 388 370 605 658 37 30 110 172 199 238 262 244 201 24 45 56

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.9 5.5 4.9 5.3 5.1 4.4 3.9 3.9 3.4 3.6 4.5 5.3 6.8 8.7 8.8 8.4
Std Deviation (1e-4) 2.0 1.5 3.8 1.5 1.4 13.1 1.3 5.7 1.3 1.0 1.0 1.1 1.8 10.6 3.0 3.6 2.8
Intensity Change (Log2) -0.15 -0.05 -0.11 -0.32 -0.49 -0.49 -0.7 -0.61 -0.29 -0.04 0.31 0.66 0.68 0.61

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3.4 0 0 0 0 0 0 0 0 0 6.4 6.0 5.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.3 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.9 2.0 -0.5 0 0 0 0 0 0 0 0 9.4 7.4 8.5