Standard name
Human Ortholog
Description Minor isoform of pyruvate decarboxylase; key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.81 0.81 0.88 0.89 0.88 0.87 0.86 – 0.81 0.79 0.7 0.82 0.77 0.9 0.9 0.43 0.54 0.58 0.65 0.68 0.62 0.57 0.79 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.1 0.09 – 0.28 0.19 0.41 0.22 0.06 0 0 0 0 0 0.07 0 0.07 0 0 0
Nucleus 0.06 0.09 0.07 0.08 0.05 0 0 – 0 0 0 0 0.09 0.1 0.09 0.17 0.17 0.09 0.12 0.14 0.11 0.22 0.05 0.06
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.15 0.05 0 0.09 0 0 – 0 0 0 0 0.11 0 0.05 0.49 0.39 0.43 0.06 0.08 0.1 0.06 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 1 1 3 0 0 0
Bud 0 0 0 0 0 2 4 – 6 2 7 6 0 0 0 0 0 0 6 4 1 0 0 0
Bud Neck 0 1 2 5 7 4 12 – 7 9 4 14 0 0 0 0 0 0 3 1 2 0 0 0
Bud Site 0 0 0 0 0 1 0 – 1 1 4 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 2 0 1 4 – 0 3 3 2 0 0 0 2 3 1 0 0 2 0 0 0
Cytoplasm 201 180 170 304 280 347 470 – 263 262 354 478 27 27 95 62 90 118 169 166 125 13 35 41
Endoplasmic Reticulum 1 0 0 0 1 0 0 – 0 3 4 0 0 0 0 5 4 3 1 0 0 0 0 0
Endosome 0 1 2 2 1 3 5 – 0 0 0 1 0 0 1 4 5 8 2 2 1 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Mitochondria 20 2 1 5 1 40 48 – 90 64 206 126 2 0 0 4 1 1 17 7 14 0 0 1
Nucleus 15 19 14 26 17 16 14 – 6 7 9 3 3 3 9 24 29 19 30 35 22 5 2 3
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 – 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 1 1 1 5 12 – 8 6 8 15 0 0 0 0 2 0 6 1 1 0 1 0
Vac/Vac Membrane 29 34 9 9 28 16 12 – 7 10 5 12 4 0 5 70 64 88 16 18 20 1 3 4
Unique Cell Count 248 223 194 342 319 399 544 325 331 504 585 35 30 105 144 166 205 262 244 201 24 45 56
Labelled Cell Count 267 240 199 354 336 436 582 388 370 605 658 37 30 110 172 199 238 262 244 201 24 45 56


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.9 5.5 4.9 5.3 5.1 4.4 – 3.9 3.9 3.4 3.6 4.5 5.3 6.8 8.7 8.8 8.4 – – –
Std Deviation (1e-4) 2.0 1.5 3.8 1.5 1.4 13.1 1.3 – 5.7 1.3 1.0 1.0 1.1 1.8 10.6 3.0 3.6 2.8 – – –
Intensity Change (Log2) – – – -0.15 -0.05 -0.11 -0.32 – -0.49 -0.49 -0.7 -0.61 -0.29 -0.04 0.31 0.66 0.68 0.61 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0 -0.2 -0.4 – -2.0 -2.5 -4.8 -1.9 -1.6 0.4 0.7 -8.7 -7.1 -6.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.3 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0.2 -0.9 -1.7 -2.9 – -3.1 -2.9 -3.6 0 0 0 0.4 2.7 3.0 0.7
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 1.8 -0.4 -1.8 – -1.6 -1.0 0 -1.9 0 0 0 9.4 8.0 8.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6658 -0.0589 -0.2176 -0.0511 -0.8824 -0.4051 -0.2904 -0.3157 -0.3881 -0.7067 -1.1147 -0.8973 -1.1379 -1.0205 -1.2034 -1.3497 -0.6651 -1.1315
Actin 0.0012 0.0004 0.0002 0.0001 0.0009 0.0007 0.0284 0.0001 0.0115 0.0003 0.0106 0.0016 0.0343 0.0001 0.0146 0.0495 0.0412 0.0074
Bud 0.0021 0.0007 0.0003 0.0012 0.0075 0.0007 0.0008 0.0007 0.0009 0.0002 0.0015 0.0032 0.0007 0.0005 0.0022 0.0002 0.0025 0.0003
Bud Neck 0.0006 0.0005 0.0002 0.0008 0.0016 0.0021 0.0051 0.0002 0.0008 0.0001 0.0018 0.0041 0.0039 0.0001 0.003 0.0003 0.0019 0.0092
Bud Periphery 0.0014 0.0011 0.0002 0.0016 0.0038 0.001 0.0016 0.0003 0.0013 0.0002 0.0018 0.0015 0.0017 0.0002 0.0015 0.0002 0.0054 0.0002
Bud Site 0.0029 0.0015 0.0021 0.0002 0.0038 0.0002 0.0045 0.0042 0.005 0.0008 0.0007 0.0003 0.0047 0.0038 0.0096 0.0004 0.0021 0.0002
Cell Periphery 0.0002 0.0001 0 0 0.0001 0 0.0002 0.0001 0.0003 0 0.0002 0.0002 0.0002 0.0001 0.0003 0.0001 0.0002 0.0001
Cytoplasm 0.0886 0.1502 0.0782 0.054 0.0982 0.1071 0.0488 0.0761 0.0805 0.0391 0.0821 0.1021 0.0513 0.1599 0.1218 0.203 0.1417 0.1784
Cytoplasmic Foci 0.0129 0.0119 0.0047 0.0033 0.0044 0.0108 0.0336 0.0013 0.0258 0.0076 0.0052 0.0164 0.0335 0.0051 0.0079 0.0064 0.0146 0.0188
Eisosomes 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0007 0.0001 0.0005 0.0001 0.0003 0.0001 0.0004 0.0006 0.0004 0.0001
Endoplasmic Reticulum 0.0015 0.0021 0.0011 0.0006 0.0012 0.0008 0.0031 0.0024 0.0009 0.0006 0.0033 0.001 0.0027 0.0012 0.0018 0.0008 0.004 0.0021
Endosome 0.0063 0.0017 0.0033 0.0007 0.0007 0.0014 0.0418 0.001 0.0186 0.0408 0.0243 0.0032 0.0119 0.0012 0.0051 0.001 0.0159 0.0029
Golgi 0.0009 0.0001 0.0001 0 0.0001 0.0001 0.0097 0 0.004 0.0084 0.0055 0.0004 0.0051 0 0.0015 0.0009 0.0107 0.001
Lipid Particles 0.0027 0.0003 0.0005 0.0001 0.0001 0.0004 0.0153 0.0001 0.0053 0.0014 0.0104 0.0011 0.0228 0.0001 0.002 0.0007 0.0094 0.0022
Mitochondria 0.0033 0.0005 0.0007 0.001 0.0004 0.0005 0.0082 0.0002 0.0079 0.0053 0.0787 0.0011 0.0022 0.0001 0.0014 0.001 0.0047 0.0004
None 0.7182 0.7015 0.7604 0.6903 0.7384 0.7223 0.6543 0.8397 0.737 0.7291 0.6892 0.7796 0.7423 0.7652 0.7219 0.7212 0.6762 0.6703
Nuclear Periphery 0.0056 0.0051 0.0039 0.0024 0.0016 0.0022 0.0201 0.0048 0.003 0.0026 0.0138 0.0026 0.0065 0.0013 0.0054 0.0008 0.0029 0.0035
Nucleolus 0.0024 0.0012 0.0014 0.0019 0.002 0.0019 0.0051 0.0007 0.0019 0.0012 0.0022 0.0025 0.0015 0.0003 0.0027 0.0002 0.0005 0.0011
Nucleus 0.1267 0.1051 0.1223 0.1847 0.1256 0.1247 0.0715 0.0658 0.0721 0.1485 0.0513 0.0489 0.034 0.0559 0.0771 0.0032 0.0479 0.0826
Peroxisomes 0.0056 0.0004 0.0002 0.0002 0.0002 0.0005 0.0167 0 0.0079 0.001 0.0023 0.0021 0.0278 0 0.0013 0.0008 0.0081 0.0007
Punctate Nuclear 0.0144 0.0128 0.0184 0.0558 0.0089 0.0219 0.0247 0.0015 0.0125 0.0094 0.0073 0.0265 0.0108 0.0028 0.0172 0.0083 0.0082 0.0177
Vacuole 0.0018 0.0022 0.0013 0.0009 0.0005 0.0005 0.0043 0.0007 0.0016 0.0023 0.004 0.0012 0.0012 0.0015 0.001 0.0003 0.0011 0.0007
Vacuole Periphery 0.0005 0.0004 0.0002 0.0003 0.0001 0.0001 0.0021 0.0001 0.0003 0.001 0.0034 0.0002 0.0005 0.0003 0.0003 0.0001 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 462.3604 568.3905 805.9057 315.6437 532.252 579.4229 449.2968 441.59 608.1423 300.9746
Translational Efficiency 0.7196 0.6469 0.4844 1.3435 0.7045 0.6551 1.1218 1.5042 0.9541 1.4827

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Minor isoform of pyruvate decarboxylase; key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism
Localization
Cell Percentages cytoplasm (30%), nucleus (20%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pdc5

Pdc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available