Standard name
Human Ortholog
Description Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.98 0.99 0.98 0.94 0.91 0.88 0.81 0.74 0.76 0.79 0.98 0.98 1.0 0.96 0.98 0.97 0.97 0.94 0.93 0.92 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.08 0.09 0.34 0.32 0.32 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 1 0 2 0
Bud 0 0 1 0 0 0 3 5 5 7 10 1 2 0 0 0 0 0 0 0 3 0 1 5
Bud Neck 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 4 5 6 5 5 6 1 1 4 4 2 1 1 9 8 5 0 0 0 0 0 0
Cytoplasm 129 252 415 372 363 364 392 466 253 237 279 267 222 243 293 410 309 334 126 250 399 163 297 250
Endoplasmic Reticulum 1 6 2 0 0 1 4 2 2 1 1 3 1 4 0 8 11 12 1 0 1 0 4 5
Endosome 0 0 0 0 0 0 0 1 1 0 1 0 0 0 1 0 2 2 0 1 3 1 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 3 0 1 1 2 2 1
Mitochondria 0 3 0 5 6 29 33 50 105 103 119 70 1 0 0 3 2 5 1 3 4 1 1 2
Nucleus 0 2 0 0 3 1 12 5 2 4 5 8 1 0 0 10 4 2 0 0 2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 2 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 3 0 1 2
Vac/Vac Membrane 0 1 4 8 5 11 4 24 5 8 9 4 2 1 1 12 5 14 0 2 2 3 5 3
Unique Cell Count 130 263 422 377 370 389 431 530 313 320 367 336 227 247 294 428 315 344 131 266 429 177 326 277
Labelled Cell Count 131 268 428 390 386 411 454 563 374 364 428 357 231 249 296 455 341 378 131 266 429 177 326 277


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 5.8 6.4 5.8 6.0 5.0 5.1 5.2 4.5 4.4 4.2 4.7 6.1 6.1 6.1 8.4 8.5 8.0 5.7 5.8 6.3
Std Deviation (1e-4) 0.7 0.8 1.0 1.0 1.0 0.9 1.0 0.9 0.8 0.7 0.8 0.6 1.3 1.1 1.4 1.2 1.2 1.2 1.2 1.5 1.5
Intensity Change (Log2) – – – -0.14 -0.07 -0.35 -0.31 -0.29 -0.49 -0.54 -0.59 -0.42 -0.07 -0.07 -0.05 0.4 0.41 0.33 -0.17 -0.12 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1.4 1.7 0
Cytoplasm 0.4 -0.2 -3.5 -4.8 -6.1 -8.2 -10.0 -9.6 -8.6 -0.5 0 1.7 -2.2 -0.3 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1 3.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.7 5.8 6.5 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 3.5 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.4 0 2.0 0 3.2 0 1.7 1.7 0 0 0 0 2.0 0 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0819 1.758 1.351 1.2182 0.4878 1.1881 1.9484 2.5547 2.167 1.7706 2.1685 2.2136 1.7848 2.7246 2.1814 1.7334 1.4223 2.3331
Actin 0.0225 0.0053 0.0028 0.0033 0.0007 0.004 0.0348 0.0021 0.0167 0.0005 0.0112 0.0076 0.0081 0.0031 0.0003 0.0002 0.0002 0.0051
Bud 0.0007 0.001 0.0006 0.0006 0.0001 0.0004 0.004 0.0074 0.001 0.0002 0.0027 0.0002 0.0005 0.0008 0.0001 0.0009 0.0001 0.0001
Bud Neck 0.0059 0.0003 0.0011 0.0004 0.0061 0.0038 0.0067 0.0005 0.0017 0.0017 0.0014 0.0012 0.0009 0.0003 0.0002 0.0005 0.0002 0.0007
Bud Periphery 0.0012 0.0011 0.0017 0.0004 0 0.0006 0.0055 0.0038 0.0008 0.0001 0.003 0.0004 0.0009 0.0004 0 0.0007 0 0.0001
Bud Site 0.0013 0.0072 0.0037 0.0015 0.0004 0.0019 0.0096 0.01 0.0094 0.0018 0.0067 0.0003 0.0011 0.002 0.0002 0.0002 0.0002 0.0001
Cell Periphery 0.0003 0.0002 0.0019 0.0001 0 0.0001 0.0004 0.0003 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.579 0.7586 0.7069 0.6397 0.4982 0.734 0.66 0.8714 0.7709 0.8634 0.7805 0.8826 0.7455 0.768 0.8087 0.9009 0.8766 0.8162
Cytoplasmic Foci 0.022 0.0181 0.0111 0.0229 0.0294 0.0165 0.0274 0.0112 0.0331 0.0353 0.0352 0.0098 0.0125 0.0092 0.0087 0.0043 0.0039 0.0118
Eisosomes 0.0003 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0089 0.0035 0.0068 0.0057 0.0015 0.0049 0.0037 0.0017 0.0028 0.0025 0.0022 0.0026 0.0034 0.0015 0.0025 0.0044 0.0021 0.0013
Endosome 0.0279 0.0033 0.0114 0.0157 0.0103 0.0096 0.0222 0.0015 0.0148 0.0051 0.0091 0.0093 0.0065 0.0024 0.0017 0.0023 0.0014 0.0021
Golgi 0.0056 0.0005 0.0012 0.0064 0.0006 0.0007 0.0107 0.0004 0.0059 0.0012 0.0063 0.0042 0.0015 0.0003 0.0001 0.0001 0.0001 0.0002
Lipid Particles 0.0048 0.0003 0.0019 0.0423 0.0018 0.0006 0.0137 0.0001 0.0058 0.0008 0.0034 0.0061 0.0029 0.0001 0.0001 0 0 0
Mitochondria 0.0075 0.0004 0.0063 0.0066 0.0003 0.0005 0.0077 0.0004 0.0038 0.0005 0.0018 0.0073 0.0008 0.0003 0.0002 0.0004 0.0001 0.0002
None 0.2604 0.1805 0.2237 0.2005 0.4337 0.1968 0.16 0.0796 0.1027 0.0769 0.0836 0.0524 0.1984 0.2051 0.1712 0.0604 0.1106 0.1534
Nuclear Periphery 0.0157 0.0014 0.0051 0.0047 0.0009 0.0097 0.0054 0.0008 0.0027 0.0008 0.0018 0.0043 0.0029 0.0006 0.0009 0.0019 0.0006 0.0006
Nucleolus 0.0006 0.0002 0.0004 0.0008 0.0013 0.0003 0.0007 0.0002 0.0054 0.0002 0.0002 0.0002 0.0006 0.0001 0 0.0001 0 0
Nucleus 0.0208 0.005 0.0065 0.0076 0.0041 0.0067 0.0095 0.0039 0.0053 0.0028 0.0105 0.0034 0.0048 0.0031 0.0032 0.0207 0.0029 0.0057
Peroxisomes 0.0021 0.0002 0.0005 0.0174 0.0006 0.0008 0.0111 0.0009 0.011 0.0034 0.0356 0.0045 0.0036 0.0005 0.0001 0.0001 0 0.001
Punctate Nuclear 0.0079 0.0108 0.0029 0.0208 0.0087 0.0061 0.002 0.0028 0.0043 0.0019 0.0033 0.0011 0.0024 0.001 0.0011 0.0006 0.0004 0.001
Vacuole 0.0033 0.0018 0.0026 0.002 0.0011 0.0017 0.0035 0.0008 0.0012 0.0007 0.0011 0.0015 0.002 0.0011 0.0005 0.0011 0.0004 0.0004
Vacuole Periphery 0.0014 0.0002 0.0007 0.0006 0.0002 0.0004 0.001 0.0001 0.0003 0.0001 0.0002 0.0008 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.447 13.4889 13.1373 18.8519 11.9218 11.3107 16.3024 19.5609 17.268 20.8327
Translational Efficiency 0.8892 0.8984 0.914 0.6831 0.8894 1.0943 0.9258 0.656 0.8577 0.679

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1768 1273 447 1052 1691 1901 335 2209 3459 3174 782 3261

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 719.34 843.50 1044.89 1026.26 733.27 825.66 1013.73 1003.66 726.15 832.82 1031.54 1010.95
Standard Deviation 75.57 112.70 122.81 134.62 81.51 102.50 109.69 134.87 78.84 107.07 118.38 135.20
Intensity Change Log 2 — 0.229714 0.538605 0.512651 — 0.171203 0.467257 0.452854 — 0.200475 0.503030 0.482775

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000050 0.000234 0.000484 0.000623 0.000342 0.000631 0.000369 0.000203 0.000193 0.000472 0.000435 0.000339
Bud Neck 0.007568 0.005226 0.002586 0.006351 0.007452 0.011268 0.002359 0.002559 0.007511 0.008845 0.002488 0.003782
Bud Site 0.002778 0.008431 0.007091 0.034805 0.004645 0.011647 0.007341 0.008910 0.003691 0.010357 0.007198 0.017264
Cell Periphery 0.000081 0.000142 0.000133 0.000134 0.000081 0.000065 0.000208 0.000056 0.000081 0.000096 0.000165 0.000081
Cytoplasm 0.665447* 0.650129* 0.453383* 0.592997* 0.617549* 0.661786* 0.479710* 0.721266* 0.642031* 0.657111* 0.464661* 0.679886*
Cytoplasmic Foci 0.102024 0.137909 0.009257 0.020973 0.156466 0.135976 0.017783 0.007435 0.128639 0.136751 0.012909 0.011802
Eisosomes 0.000058 0.000055 0.000031 0.000024 0.000075 0.000047 0.000082 0.000009 0.000066 0.000050 0.000053 0.000014
Endoplasmic Reticulum 0.000437 0.000969 0.003922 0.001585 0.001076 0.000409 0.004170 0.000587 0.000749 0.000634 0.004028 0.000909
Endosome 0.003859 0.011604 0.002235 0.006727 0.008084 0.009800 0.006087 0.000939 0.005924 0.010524 0.003885 0.002806
Golgi 0.000667 0.001438 0.000129 0.008745 0.001150 0.002378 0.000141 0.000398 0.000903 0.002001 0.000134 0.003091
Lipid Particles 0.002033 0.002532 0.001190 0.001154 0.002966 0.001529 0.002741 0.000394 0.002489 0.001932 0.001854 0.000639
Mitochondria 0.001609 0.001974 0.000388 0.000868 0.001632 0.002679 0.000425 0.000442 0.001620 0.002396 0.000404 0.000579
Mitotic Spindle 0.001891 0.002763 0.003272 0.054625 0.002117 0.011908 0.002677 0.022997 0.002001 0.008240 0.003017 0.033200
None 0.006721 0.005835 0.005846 0.005522 0.002902 0.002045 0.005280 0.009972 0.004854 0.003565 0.005603 0.008536
Nuclear Periphery 0.000209 0.000301 0.001116 0.001203 0.000280 0.000172 0.001504 0.000449 0.000244 0.000223 0.001282 0.000692
Nuclear Periphery Foci 0.000293 0.001477 0.003904 0.004553 0.000813 0.000433 0.005110 0.001318 0.000547 0.000852 0.004421 0.002362
Nucleolus 0.000987 0.000952 0.000184 0.000152 0.001089 0.000782 0.000564 0.000600 0.001037 0.000850 0.000347 0.000455
Nucleus 0.158236* 0.085897 0.407868* 0.134591* 0.118648 0.073766 0.257260* 0.179034* 0.138882* 0.078631 0.343349* 0.164697*
Peroxisomes 0.000951 0.003095 0.000169 0.001037 0.002251 0.004359 0.000293 0.000117 0.001587 0.003852 0.000222 0.000414
Vacuole 0.043704 0.078237 0.096663 0.122405 0.069731 0.067533 0.205537* 0.042128 0.056428 0.071826 0.143304 0.068026
Vacuole Periphery 0.000397 0.000798 0.000150 0.000928 0.000649 0.000786 0.000359 0.000186 0.000520 0.000791 0.000240 0.000425

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.21 -9.40 -9.69 -4.79 3.32 -0.73 -0.53 0.75 1.21 5.06 -1.24 -4.49 -1.18 0.83 6.45
Bud Neck 2.33 3.78 3.96 2.13 0.07 -3.31 6.09 6.60 8.48 -0.30 -1.57 6.46 7.41 8.70 0.42
Bud Site -5.03 -2.45 -5.97 -2.80 -2.72 -6.16 -1.40 -1.96 4.23 0.54 -8.21 -2.70 -6.15 1.30 -1.00
Cell Periphery -1.61 -7.97 -4.13 0.93 5.44 2.32 -7.59 3.67 2.18 10.67 -0.93 -10.85 2.08 1.51 12.26
Cytoplasm 2.51 24.38 21.35 17.20 -6.89 -5.09 15.71 1.24 6.74 -15.41 -1.90 28.21 13.82 14.79 -19.21
Cytoplasmic Foci -6.44 27.78 26.08 26.88 -3.51 3.48 32.23 34.05 32.52 3.59 -2.04 42.02 42.87 42.37 0.50
Eisosomes 0.44 2.31 6.94 6.78 3.96 2.25 -0.99 4.85 15.89 13.47 2.43 0.17 7.15 16.76 14.30
Endoplasmic Reticulum -3.77 -12.38 -11.57 -4.78 9.14 3.54 -9.47 2.29 -2.74 11.19 0.97 -15.36 -3.06 -4.47 14.88
Endosome -7.43 2.40 3.00 8.54 1.26 -1.68 4.06 12.71 12.67 9.56 -6.75 5.11 13.36 15.37 9.35
Golgi -1.91 2.27 -1.03 -0.20 -1.63 -2.41 7.60 6.56 4.50 -0.79 -3.14 5.79 0.87 3.17 -1.70
Lipid Particles -1.46 0.68 2.53 3.41 2.07 4.54 1.43 11.73 4.05 8.98 2.38 1.77 11.34 5.70 9.70
Mitochondria -0.28 2.74 2.84 1.61 0.68 -1.36 2.69 2.59 3.88 -0.75 -1.21 3.82 3.84 3.84 0.12
Mitotic Spindle -0.53 -1.91 -6.95 -6.56 -4.46 -4.53 -0.43 -8.99 -3.47 -8.81 -4.36 -1.95 -11.41 -6.89 -7.71
None 0.85 2.60 2.22 0.53 -0.58 2.19 -1.84 -13.79 -17.81 -8.57 2.33 0.81 -7.21 -8.94 -8.85
Nuclear Periphery -2.90 -15.98 -13.32 -12.01 1.64 4.17 -15.74 -18.13 -24.65 8.66 0.75 -21.95 -21.93 -22.01 8.22
Nuclear Periphery Foci -8.49 -10.88 -9.72 -5.76 2.80 4.91 -6.07 -3.95 -7.05 5.24 -4.62 -11.54 -10.72 -8.02 7.31
Nucleolus 0.11 3.17 3.41 4.76 1.56 1.16 2.32 1.17 0.42 -0.56 1.05 3.76 1.83 1.30 -0.67
Nucleus 15.60 -19.58 -1.83 -13.88 17.92 11.98 -12.33 -21.64 -33.44 3.89 20.41 -22.66 -17.65 -35.11 14.79
Peroxisomes -3.39 3.83 2.78 4.47 -1.57 -3.39 7.49 7.58 7.32 0.94 -5.07 8.29 7.97 8.60 -0.31
Vacuole -11.07 -12.44 -28.94 -23.01 -8.18 0.60 -14.60 -8.47 -9.27 11.96 -7.38 -18.49 -26.23 -21.19 6.01
Vacuole Periphery -4.33 4.23 2.15 5.94 -1.00 -0.78 3.40 6.46 4.61 2.09 -2.51 5.21 7.10 6.70 1.78
Standard name
Human Ortholog
Description Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (78%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Acf2

Acf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Acf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available