ORF
Human Ortholog
Description Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.98 0.97 – 0.93 0.92 0.9 0.78 0.82 0.67 0.8 1.0 0.99 0.98 0.93 0.91 0.92 0.92 0.92 0.74 0.99 0.88 0.89
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0.08 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 – 0.08 0.07 0.09 0.25 0.15 0.42 0.22 0 0 0 0 0 0 0 0 0.16 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 – 0 0 0 0 0.06 0 0.06 0 0 0 0 0.05 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 – 0 1 3 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 2
Bud 1 0 0 0 – 3 2 2 7 4 3 5 0 0 0 0 0 0 0 2 0 0 0 2
Bud Neck 0 0 0 2 – 4 8 2 0 0 0 1 0 0 0 0 0 0 1 0 0 0 1 0
Bud Site 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 6 0 1 – 4 4 2 2 5 3 6 0 0 0 4 6 3 0 1 1 0 0 0
Cytoplasm 128 408 59 149 – 299 411 485 256 304 226 265 21 89 135 164 173 162 128 386 70 34 85 145
Endoplasmic Reticulum 1 3 0 0 – 1 3 1 2 4 1 0 0 0 2 16 15 15 0 0 0 0 0 0
Endosome 0 1 0 0 – 1 1 6 7 3 10 5 0 0 0 4 5 4 0 4 1 0 1 1
Golgi 1 1 0 0 – 0 0 0 1 1 0 0 0 0 1 3 0 0 0 1 0 0 0 0
Mitochondria 7 2 1 5 – 27 32 50 81 54 142 74 0 0 0 7 6 5 4 9 15 0 0 2
Nucleus 0 1 1 1 – 2 0 2 1 1 2 2 0 0 0 0 0 0 0 1 1 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 – 0 0 2 0 0 0 0 0 1 0 0 2 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 1 3 0 1 1 5 3 0 0 0 0 1 1 0 1 0 0 1 1
Vac/Vac Membrane 0 0 0 0 – 5 11 16 7 24 10 19 0 1 0 2 9 10 0 3 2 0 0 1
Unique Cell Count 133 416 60 154 321 445 540 330 372 339 333 21 90 138 177 191 177 139 420 96 35 97 163
Labelled Cell Count 139 422 61 159 347 477 571 365 401 403 380 21 91 138 200 218 202 139 420 96 35 97 163


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 5.0 4.6 5.1 – 5.2 5.3 5.7 4.7 4.9 4.5 4.8 4.8 5.5 6.1 6.4 6.6 6.4 – 4.8 5.8
Std Deviation (1e-4) 0.5 0.9 0.4 1.7 – 1.6 1.4 1.6 1.2 1.1 1.3 1.3 0.7 0.9 1.3 1.7 2.1 2.1 – 1.2 1.8
Intensity Change (Log2) – – – 0.17 – 0.18 0.22 0.31 0.04 0.1 -0.03 0.06 0.07 0.26 0.41 0.49 0.53 0.48 – 0.07 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 – -1.5 -1.7 -2.2 -3.8 -3.3 -5.0 -3.5 0.6 0.3 -0.2 -1.6 -2.0 -1.8
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 2.0 0 2.8 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0871 0.0023 -0.1827 -0.4814 0.4922 -0.1771 -0.0192 0.4623 0.1861 0.3883 0.3533 0.2484 -0.5347 -0.2975 -0.6069 -0.5544 -0.5969 -0.6312
Actin 0.0193 0.0088 0.0136 0.002 0.0036 0.0094 0.0103 0.0001 0.0081 0.0073 0.017 0.0112 0.03 0.0006 0.0193 0.0308 0.0187 0.0023
Bud 0.0089 0.002 0.0065 0.0017 0.0013 0.0008 0.0021 0.0001 0.0008 0.0009 0.0044 0.0003 0.0006 0.0017 0.0014 0.0047 0.0082 0.0013
Bud Neck 0.0211 0.0003 0.0128 0.0002 0.0015 0.0003 0.0047 0.0001 0.0027 0.0036 0.0011 0.0007 0.0139 0.0002 0.0007 0.0002 0.0018 0.0016
Bud Periphery 0.0101 0.0026 0.0024 0.007 0.001 0.0033 0.0052 0.0001 0.0013 0.0008 0.0095 0.0005 0.0012 0.0025 0.0015 0.0047 0.0154 0.0018
Bud Site 0.021 0.0121 0.0206 0.0006 0.0293 0.0002 0.004 0.0018 0.0037 0.0044 0.0124 0.0003 0.0063 0.0045 0.0057 0.0004 0.0085 0.0006
Cell Periphery 0.0005 0.0002 0.0007 0.0002 0.0005 0.0001 0.0004 0.0002 0.0005 0.0001 0.0003 0.0001 0.0006 0.0001 0.0002 0.0001 0.0002 0.0001
Cytoplasm 0.1364 0.257 0.1861 0.2251 0.1401 0.1898 0.3637 0.4685 0.3526 0.3354 0.383 0.4212 0.2391 0.2912 0.2436 0.305 0.1539 0.3289
Cytoplasmic Foci 0.0629 0.0071 0.0242 0.0227 0.0257 0.0152 0.0197 0.0045 0.0166 0.0352 0.0202 0.0193 0.0249 0.0139 0.0102 0.0073 0.0396 0.0188
Eisosomes 0.0004 0.0002 0.0004 0.0002 0.0017 0.0002 0.0005 0.0001 0.0004 0.0002 0.0002 0.0001 0.0007 0 0.0002 0.0001 0.0002 0
Endoplasmic Reticulum 0.0063 0.0031 0.0029 0.0029 0.0066 0.0009 0.0034 0.0022 0.0026 0.0017 0.0027 0.0023 0.004 0.001 0.0037 0.002 0.002 0.0014
Endosome 0.0464 0.0016 0.0123 0.014 0.0102 0.0032 0.0082 0.0017 0.014 0.0098 0.0161 0.009 0.0126 0.0013 0.006 0.0034 0.0322 0.0075
Golgi 0.0147 0.0003 0.0065 0.0009 0.0051 0.0035 0.0019 0.0001 0.0041 0.0025 0.0037 0.0026 0.0035 0.0001 0.0013 0.0005 0.0107 0.0011
Lipid Particles 0.0187 0.0005 0.0047 0.0009 0.0161 0.0073 0.0064 0.0002 0.0041 0.0177 0.0441 0.0015 0.0084 0.0002 0.0014 0.0002 0.0228 0.0019
Mitochondria 0.0204 0.0004 0.0088 0.007 0.0054 0.0122 0.0012 0.0002 0.0104 0.0013 0.0027 0.0021 0.0054 0.0002 0.0012 0.0006 0.0058 0.0013
None 0.5542 0.6988 0.6567 0.691 0.6186 0.744 0.5397 0.5171 0.5588 0.5248 0.4709 0.5192 0.6092 0.6733 0.6839 0.6303 0.6199 0.6086
Nuclear Periphery 0.0047 0.0007 0.0026 0.0022 0.0329 0.0009 0.0058 0.0004 0.001 0.0008 0.0014 0.0023 0.0056 0.0006 0.0052 0.0014 0.0071 0.0016
Nucleolus 0.0033 0.0001 0.0019 0.0005 0.0085 0.0001 0.0005 0.0001 0.0004 0.0016 0.0003 0.0003 0.0011 0.0002 0.0004 0.0006 0.0015 0.0028
Nucleus 0.0049 0.0009 0.0035 0.0022 0.008 0.0005 0.0027 0.0009 0.0012 0.0027 0.0012 0.0018 0.0109 0.0025 0.0027 0.0029 0.0037 0.0035
Peroxisomes 0.0206 0.0018 0.0122 0.0111 0.0624 0.0051 0.0141 0 0.012 0.0346 0.002 0.0017 0.0098 0.0008 0.0058 0.0006 0.0271 0.0012
Punctate Nuclear 0.0186 0.0008 0.007 0.0048 0.0169 0.002 0.0033 0.0003 0.0009 0.0134 0.001 0.0011 0.0093 0.0037 0.0037 0.0026 0.018 0.0106
Vacuole 0.005 0.0008 0.0112 0.0023 0.0025 0.0004 0.0018 0.0012 0.0031 0.001 0.0048 0.0016 0.002 0.0012 0.0013 0.0014 0.0018 0.0023
Vacuole Periphery 0.0018 0.0001 0.0022 0.0005 0.0022 0.0003 0.0003 0.0001 0.0006 0.0002 0.0012 0.0006 0.0008 0.0001 0.0004 0.0003 0.0007 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.3093 6.2727 12.4122 11.0321 14.7837 12.8572 16.12 20.3329 19.7376 16.6941
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
78 1558 361 107 1347 2112 157 1502 1425 3670 518 1609

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 628.71 695.11 994.68 954.49 645.56 700.67 1040.53 696.01 644.64 698.31 1008.58 713.20
Standard Deviation 58.94 102.93 120.66 129.35 75.02 88.51 117.44 78.47 74.33 94.94 121.53 104.92
Intensity Change Log 2 — 0.144847 0.661838 0.602335 — 0.118184 0.688695 0.108557 — 0.131401 0.675507 0.373232

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000102 0.000190 0.000935 0.000388 0.000075 0.000293 0.000619 0.000064 0.000076 0.000249 0.000839 0.000086
Bud Neck 0.008480 0.015419 0.005169 0.001497 0.011207 0.026517 0.002351 0.004717 0.011058 0.021805 0.004315 0.004503
Bud Site 0.000957 0.008621 0.011176 0.006934 0.001948 0.013177 0.005532 0.003531 0.001894 0.011243 0.009466 0.003758
Cell Periphery 0.000310 0.000088 0.000292 0.000257 0.000137 0.000136 0.000334 0.000448 0.000147 0.000115 0.000305 0.000435
Cytoplasm 0.460866* 0.546234* 0.439439* 0.404448* 0.485419* 0.491744* 0.446068* 0.509693* 0.484075* 0.514876* 0.441448* 0.502694*
Cytoplasmic Foci 0.301822* 0.166751 0.026934 0.043893 0.255819* 0.203928* 0.020204 0.047021 0.258337* 0.188145* 0.024894 0.046813
Eisosomes 0.000330 0.000076 0.000086 0.000109 0.000179 0.000110 0.000101 0.000170 0.000187 0.000095 0.000091 0.000166
Endoplasmic Reticulum 0.001384 0.000751 0.008478 0.004908 0.000686 0.000554 0.008377 0.000369 0.000724 0.000637 0.008447 0.000670
Endosome 0.012213 0.015191 0.009305 0.011316 0.012043 0.019048 0.008361 0.003310 0.012053 0.017411 0.009019 0.003843
Golgi 0.001686 0.001941 0.000230 0.000155 0.002037 0.003131 0.000192 0.000626 0.002018 0.002626 0.000218 0.000594
Lipid Particles 0.018755 0.005520 0.003689 0.004352 0.011588 0.005621 0.004261 0.002053 0.011981 0.005578 0.003863 0.002206
Mitochondria 0.004613 0.004249 0.001427 0.000153 0.005793 0.006542 0.000263 0.001694 0.005728 0.005569 0.001074 0.001591
Mitotic Spindle 0.000026 0.002444 0.009688 0.000401 0.000368 0.006431 0.007339 0.002384 0.000349 0.004739 0.008976 0.002252
None 0.002357 0.004051 0.002792 0.002154 0.002875 0.004158 0.003808 0.007567 0.002847 0.004112 0.003100 0.007207
Nuclear Periphery 0.000894 0.000534 0.002002 0.001272 0.000638 0.000487 0.002055 0.000631 0.000652 0.000507 0.002018 0.000674
Nuclear Periphery Foci 0.000260 0.000657 0.007716 0.010261 0.000303 0.000268 0.009747 0.000237 0.000301 0.000433 0.008332 0.000904
Nucleolus 0.009518 0.002053 0.000465 0.000374 0.000979 0.001870 0.000369 0.000948 0.001446 0.001948 0.000436 0.000910
Nucleus 0.136451* 0.143034* 0.260096* 0.271142* 0.160232* 0.135425* 0.231759* 0.373468* 0.158930* 0.138655* 0.251508* 0.366663*
Peroxisomes 0.004223 0.005869 0.000662 0.000456 0.006126 0.007316 0.000367 0.001040 0.006022 0.006702 0.000572 0.001001
Vacuole 0.033586 0.074597 0.208812* 0.235298* 0.040619 0.071261 0.247635* 0.039643 0.040234 0.072677 0.220579* 0.052654
Vacuole Periphery 0.001168 0.001729 0.000606 0.000232 0.000930 0.001984 0.000259 0.000387 0.000943 0.001876 0.000501 0.000377

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.55 -8.21 -4.25 -4.25 5.72 -2.74 -9.01 0.51 2.81 8.91 -3.72 -11.71 -0.66 3.29 11.31
Bud Neck -3.33 1.57 4.43 10.44 3.68 -8.35 6.85 4.88 13.43 -1.91 -7.30 4.78 5.20 14.22 0.38
Bud Site -6.63 -4.77 -2.63 0.87 1.53 -8.78 -6.80 -2.14 6.69 2.88 -10.47 -5.15 -2.61 6.83 3.60
Cell Periphery 2.52 0.14 1.07 -4.67 2.72 0.20 -5.97 -6.79 -6.68 -2.89 3.05 -8.99 -6.62 -7.45 -2.82
Cytoplasm -2.78 3.23 3.25 8.02 0.65 -0.60 8.55 -1.98 -1.51 -9.45 -3.42 10.48 -1.07 2.18 -11.25
Cytoplasmic Foci 6.11 12.95 12.38 23.06 -2.58 7.29 40.40 34.90 32.32 -12.56 10.99 41.85 36.72 38.19 -11.56
Eisosomes 5.21 4.71 4.46 -2.96 -0.76 8.00 5.68 0.91 -3.45 -2.82 11.01 9.12 1.69 -4.46 -3.72
Endoplasmic Reticulum 1.02 -9.25 -4.02 -6.64 5.57 0.95 -9.95 2.07 2.23 10.31 0.59 -15.83 0.08 -0.95 16.12
Endosome -1.05 1.10 1.21 3.73 0.39 -4.68 3.70 7.61 14.32 5.42 -4.13 2.93 7.65 16.22 7.84
Golgi -0.34 2.67 2.86 5.94 4.19 -2.42 7.82 4.34 5.69 -1.77 -1.73 7.93 4.63 6.13 -1.56
Lipid Particles 3.39 3.78 3.84 2.56 0.58 6.37 7.93 10.80 9.11 4.57 7.16 9.06 11.38 10.49 7.32
Mitochondria 0.32 2.61 3.45 6.30 2.22 -0.52 4.41 3.18 5.96 -3.12 0.16 4.02 3.39 6.38 -1.39
Mitotic Spindle -4.02 -2.49 -2.26 2.08 2.22 -5.47 -1.68 -2.22 3.04 0.93 -6.25 -2.80 -2.28 2.47 2.00
None -1.91 0.09 0.71 3.82 1.12 -1.77 0.53 -4.57 -3.04 -4.69 -2.14 1.01 -4.50 -3.24 -5.53
Nuclear Periphery 0.90 -3.91 -1.49 -7.78 6.07 1.39 -9.75 -0.53 -3.66 10.47 1.41 -13.93 -0.93 -5.13 17.42
Nuclear Periphery Foci -4.83 -13.28 -4.82 -4.55 0.04 0.81 -8.26 0.81 -0.00 8.30 -3.10 -15.55 -3.92 -2.72 14.23
Nucleolus 1.46 1.78 1.83 7.61 3.26 -3.45 6.59 0.16 3.17 -3.70 -1.68 3.50 1.59 4.44 -2.85
Nucleus -0.48 -6.68 -3.40 -3.84 1.83 4.27 -4.76 -25.85 -30.91 -6.58 3.84 -9.60 -25.73 -31.54 -8.46
Peroxisomes -1.50 3.68 3.98 8.57 1.99 -1.30 8.62 7.23 10.58 -3.14 -0.85 8.51 7.54 12.29 -1.94
Vacuole -4.85 -15.41 -11.01 -9.84 -2.51 -11.28 -14.99 -2.47 7.48 14.64 -14.17 -22.98 -7.31 1.84 19.94
Vacuole Periphery -1.47 2.40 3.84 6.23 2.72 -3.81 5.04 3.61 6.49 -2.07 -4.23 3.40 3.98 8.20 0.58
ORF
Human Ortholog
Description Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YLR177W

YLR177W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr177w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available