Standard name | |
---|---|
Human Ortholog | |
Description | Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; localizes to vacuole membrane in response to H2O2; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 8 | 3 | 4 | 3 | 4 | 4 | 3 | 1 | 7 | 6 | 10 | 3 | 2 | 10 | 15 | 13 | 15 | 2 | 7 | 18 | 4 | 11 | 14 |
Bud | 1 | 4 | 5 | 4 | 5 | 7 | 8 | 12 | 9 | 14 | 7 | 14 | 1 | 1 | 1 | 6 | 5 | 5 | 1 | 1 | 2 | 2 | 5 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Bud Site | 1 | 1 | 1 | 0 | 0 | 3 | 4 | 0 | 1 | 1 | 4 | 5 | 0 | 0 | 0 | 1 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 11 | 12 | 9 | 4 | 2 | 6 | 5 | 5 | 8 | 6 | 10 | 10 | 29 | 6 | 21 | 7 | 15 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 2 | 0 | 6 | 11 | 9 | 24 | 33 | 13 | 13 | 11 | 13 | 5 | 5 | 11 | 15 | 23 | 10 | 13 | 0 | 0 | 0 | 3 | 1 | 3 |
Endoplasmic Reticulum | 0 | 3 | 6 | 3 | 10 | 32 | 37 | 28 | 20 | 42 | 34 | 32 | 2 | 4 | 5 | 8 | 0 | 4 | 0 | 0 | 0 | 1 | 1 | 1 |
Endosome | 1 | 1 | 6 | 4 | 10 | 3 | 3 | 10 | 9 | 4 | 3 | 0 | 7 | 4 | 5 | 3 | 2 | 3 | 5 | 6 | 8 | 4 | 12 | 6 |
Golgi | 0 | 12 | 26 | 21 | 23 | 8 | 8 | 22 | 28 | 21 | 9 | 16 | 112 | 332 | 275 | 132 | 39 | 72 | 6 | 35 | 29 | 46 | 63 | 49 |
Mitochondria | 184 | 465 | 417 | 393 | 466 | 650 | 759 | 613 | 680 | 847 | 748 | 804 | 183 | 324 | 318 | 23 | 5 | 10 | 172 | 411 | 453 | 267 | 381 | 445 |
Nucleus | 0 | 0 | 2 | 0 | 1 | 2 | 4 | 4 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 3 | 1 | 3 | 0 | 0 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 10 | 17 | 37 | 20 | 54 | 98 | 103 | 97 | 63 | 95 | 72 | 85 | 6 | 19 | 7 | 10 | 7 | 1 | 2 | 3 | 3 | 2 | 6 | 7 |
Nucleolus | 20 | 31 | 27 | 21 | 63 | 113 | 118 | 98 | 80 | 83 | 70 | 61 | 11 | 8 | 5 | 9 | 8 | 9 | 2 | 4 | 3 | 3 | 5 | 4 |
Peroxisomes | 4 | 7 | 51 | 19 | 14 | 12 | 11 | 36 | 20 | 17 | 10 | 17 | 4 | 2 | 9 | 17 | 8 | 17 | 6 | 9 | 40 | 4 | 15 | 11 |
SpindlePole | 0 | 1 | 3 | 2 | 6 | 7 | 6 | 11 | 3 | 3 | 8 | 3 | 2 | 1 | 1 | 1 | 1 | 2 | 2 | 0 | 2 | 2 | 3 | 2 |
Vac/Vac Membrane | 23 | 39 | 12 | 11 | 24 | 19 | 14 | 31 | 19 | 25 | 21 | 17 | 35 | 111 | 60 | 40 | 13 | 30 | 2 | 20 | 0 | 19 | 21 | 21 |
Unique Cell Count | 199 | 486 | 494 | 443 | 574 | 776 | 931 | 814 | 802 | 989 | 846 | 906 | 253 | 547 | 472 | 257 | 101 | 168 | 206 | 503 | 568 | 368 | 536 | 577 |
Labelled Cell Count | 248 | 600 | 614 | 522 | 692 | 985 | 1118 | 983 | 951 | 1181 | 1013 | 1080 | 381 | 849 | 717 | 312 | 120 | 199 | 206 | 503 | 568 | 368 | 536 | 577 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 14.5 | 14.8 | 14.2 | 14.8 | 14.2 | 14.5 | 15.0 | 15.2 | 15.3 | 14.4 | 14.6 | 14.0 | 14.0 | 13.4 | 18.5 | 16.3 | 17.9 | 15.1 | 15.0 | 16.6 |
Std Deviation (1e-4) | 2.1 | 3.2 | 4.6 | 4.3 | 5.5 | 5.5 | 5.5 | 5.2 | 5.7 | 5.8 | 5.4 | 5.5 | 2.9 | 2.6 | 2.4 | 5.5 | 4.4 | 5.7 | 3.5 | 3.4 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.0 | -0.06 | -0.03 | 0.02 | 0.04 | 0.05 | -0.04 | -0.02 | -0.08 | -0.08 | -0.14 | 0.32 | 0.14 | 0.27 | 0.03 | 0.02 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 4.4 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0.7 | 0.2 | 0.7 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.4 | -2.3 | -3.6 | -2.9 | -2.8 | -2.8 | -2.5 | -2.6 | -1.9 | 1.2 | 2.4 | -1.3 | 3.6 | 0 | 3.7 |
Cytoplasm | 1.5 | 0.5 | 2.1 | 2.6 | 0.6 | 0.6 | -0.2 | 0.5 | 0 | 0 | 1.0 | 2.1 | 5.2 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0.7 | 3.0 | 2.9 | 2.5 | 1.6 | 3.1 | 2.9 | 2.5 | 0 | 0 | -0.2 | 0 | 0 | 0 |
Endosome | 0 | 0.7 | 0 | 0 | 0 | -0.2 | 0 | 0 | 0 | 0 | 0 | -0.2 | 0 | 0 | 0 |
Golgi | -0.4 | -1.0 | -4.6 | -5.2 | -2.4 | -1.6 | -3.3 | -4.6 | -3.7 | 13.0 | 18.8 | 17.8 | 14.7 | 9.8 | 11.9 |
Mitochondria | 1.9 | -1.4 | -0.3 | -1.4 | -3.9 | 0.2 | 0.6 | 2.1 | 2.3 | -3.9 | -9.0 | -6.2 | -19.9 | -16.0 | -18.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.9 | 1.1 | 2.9 | 2.2 | 2.6 | 0.2 | 1.3 | 0.7 | 1.2 | -2.8 | -2.9 | -4.5 | -1.9 | -0.2 | -3.3 |
Nucleolus | -0.5 | 3.2 | 5.0 | 4.3 | 3.9 | 2.9 | 2.0 | 1.9 | 0.9 | -0.7 | -3.6 | -3.8 | -1.2 | 1.0 | -0.1 |
Peroxisomes | -3.5 | -5.4 | -7.0 | -8.1 | -4.2 | -6.0 | -7.5 | -7.7 | -7.0 | -4.3 | -7.3 | -5.4 | -1.7 | -0.7 | -0.1 |
SpindlePole | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 1.6 | 0 | -1.2 | 1.4 | -0.1 | 0.1 | 0.1 | -0.7 | 6.1 | 8.9 | 6.1 | 6.7 | 0 | 7.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.0998 | 15.3386 | 14.5408 | 14.1707 | 12.5247 | 14.341 | 12.5244 | 17.0624 | 15.251 | 14.018 | 12.5504 | 14.9216 | 16.0512 | 15.2822 | 15.5591 | 14.116 | 14.9155 | 15.4596 |
Actin | 0.0327 | 0.002 | 0.0103 | 0.0001 | 0.0013 | 0.0003 | 0.0591 | 0.0076 | 0.0114 | 0.0338 | 0.0425 | 0.0068 | 0.0039 | 0.0041 | 0.0027 | 0.0002 | 0.0001 | 0.0001 |
Bud | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0003 | 0 | 0.0005 | 0.0003 | 0.0001 | 0.0006 | 0.0011 | 0 | 0.0003 | 0.0014 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0.0061 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Periphery | 0.0004 | 0.0002 | 0.002 | 0 | 0.0008 | 0 | 0.0006 | 0.0011 | 0.0003 | 0.0031 | 0.0023 | 0.0001 | 0.0004 | 0.0013 | 0.0003 | 0.0001 | 0 | 0 |
Bud Site | 0.0007 | 0.0002 | 0.0006 | 0 | 0.0004 | 0.0001 | 0.0014 | 0.0004 | 0.0003 | 0.0024 | 0.0017 | 0 | 0.0022 | 0.0019 | 0.0004 | 0 | 0 | 0 |
Cell Periphery | 0.0002 | 0.0003 | 0.0009 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0014 | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0022 | 0 | 0 | 0.0003 | 0.0002 | 0 | 0.0018 | 0 | 0 | 0.0002 | 0 | 0 |
Cytoplasmic Foci | 0.0039 | 0.0019 | 0.0021 | 0 | 0.0012 | 0.0003 | 0.012 | 0.0007 | 0.0003 | 0.0037 | 0.0095 | 0.0003 | 0.0073 | 0.002 | 0.0007 | 0.0007 | 0 | 0 |
Eisosomes | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0.0003 | 0.0003 | 0.0004 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0005 | 0.0002 | 0.0033 | 0 | 0.0001 | 0.0004 | 0.0013 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endosome | 0.0058 | 0.0087 | 0.01 | 0.0003 | 0.0111 | 0.0008 | 0.0166 | 0.0043 | 0.0024 | 0.0042 | 0.0178 | 0.0031 | 0.0039 | 0.0035 | 0.0011 | 0.008 | 0.0002 | 0.0002 |
Golgi | 0.0075 | 0.0195 | 0.0127 | 0.0038 | 0.0045 | 0.0028 | 0.0089 | 0.0163 | 0.0028 | 0.0075 | 0.0196 | 0.006 | 0.0072 | 0.0112 | 0.0022 | 0.013 | 0.001 | 0.001 |
Lipid Particles | 0.0203 | 0.0025 | 0.0028 | 0.0007 | 0.007 | 0.0068 | 0.0118 | 0.0042 | 0.0031 | 0.0055 | 0.028 | 0.0042 | 0.0153 | 0.003 | 0.0024 | 0.0001 | 0 | 0.0012 |
Mitochondria | 0.905 | 0.9247 | 0.929 | 0.9931 | 0.9634 | 0.9799 | 0.8113 | 0.9463 | 0.9656 | 0.9081 | 0.8454 | 0.9691 | 0.9235 | 0.9574 | 0.9812 | 0.9763 | 0.9982 | 0.9919 |
None | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0022 | 0 | 0 | 0.0003 | 0.0007 | 0.0001 | 0.0012 | 0 | 0.0002 | 0 | 0 | 0 |
Nuclear Periphery | 0.0002 | 0.0045 | 0.0013 | 0 | 0.0015 | 0.0007 | 0.0307 | 0 | 0.0001 | 0.0002 | 0.0075 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 |
Nucleolus | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.001 | 0.0023 | 0.002 | 0.0001 | 0.0049 | 0.001 | 0.0006 | 0.0045 | 0.0004 | 0.0015 | 0 | 0 | 0 |
Nucleus | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0023 | 0.0038 | 0.0002 | 0.0001 | 0.0008 | 0.001 | 0.0003 | 0.0011 | 0.0001 | 0.0004 | 0 | 0 | 0 |
Peroxisomes | 0.0168 | 0.0023 | 0.0018 | 0.0002 | 0.0011 | 0.0002 | 0.0078 | 0.0021 | 0.0009 | 0.0044 | 0.0082 | 0.0006 | 0.0032 | 0.0072 | 0.0014 | 0.0004 | 0 | 0.0003 |
Punctate Nuclear | 0.0002 | 0 | 0.0011 | 0 | 0.0002 | 0.0012 | 0.0171 | 0 | 0 | 0.0003 | 0.0009 | 0.0001 | 0.0007 | 0 | 0.0004 | 0 | 0 | 0 |
Vacuole | 0.0014 | 0.0086 | 0.0112 | 0.0002 | 0.0032 | 0.0004 | 0.0015 | 0.0024 | 0.0022 | 0.0041 | 0.0022 | 0.0015 | 0.0044 | 0.0014 | 0.0007 | 0.0002 | 0 | 0.0002 |
Vacuole Periphery | 0.0033 | 0.0239 | 0.0107 | 0.0014 | 0.0025 | 0.0022 | 0.005 | 0.0114 | 0.0098 | 0.0076 | 0.0077 | 0.0061 | 0.0178 | 0.0044 | 0.0034 | 0.0004 | 0.0003 | 0.0047 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.8158 | 30.8478 | 38.5228 | 51.8718 | 37.1021 | 39.5357 | 50.2085 | 59.0462 | 58.9631 | 56.5147 |
Translational Efficiency | 0.8102 | 1.0049 | 0.8724 | 0.7182 | 0.9044 | 1.1607 | 0.8271 | 0.7609 | 0.9116 | 0.7867 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1744 | 701 | 2292 | 1965 | 1694 | 1940 | 495 | 2992 | 3438 | 2641 | 2787 | 4957 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1106.00 | 1265.88 | 1480.79 | 1666.97 | 1138.94 | 1189.35 | 1587.31 | 1673.04 | 1122.23 | 1209.66 | 1499.71 | 1670.63 |
Standard Deviation | 218.83 | 286.18 | 310.92 | 305.47 | 226.66 | 230.76 | 304.45 | 315.95 | 223.33 | 248.99 | 312.44 | 311.85 |
Intensity Change Log 2 | — | 0.194789 | 0.421016 | 0.591877 | — | 0.062482 | 0.478892 | 0.554780 | — | 0.129181 | 0.450669 | 0.573176 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001769 | 0.006281 | 0.001191 | 0.005527 | 0.000778 | 0.003064 | 0.006019 | 0.001534 | 0.001281 | 0.003918 | 0.002048 | 0.003117 |
Bud Neck | 0.000719 | 0.001801 | 0.000988 | 0.000694 | 0.000705 | 0.001883 | 0.005222 | 0.000252 | 0.000712 | 0.001861 | 0.001740 | 0.000427 |
Bud Site | 0.000874 | 0.002024 | 0.000966 | 0.001126 | 0.001049 | 0.002311 | 0.004160 | 0.000596 | 0.000960 | 0.002235 | 0.001533 | 0.000806 |
Cell Periphery | 0.000193 | 0.000185 | 0.000099 | 0.000193 | 0.000157 | 0.000317 | 0.000118 | 0.000316 | 0.000175 | 0.000282 | 0.000102 | 0.000267 |
Cytoplasm | 0.000107 | 0.000404 | 0.000276 | 0.000271 | 0.000144 | 0.000269 | 0.000849 | 0.000062 | 0.000126 | 0.000305 | 0.000378 | 0.000145 |
Cytoplasmic Foci | 0.004547 | 0.022446 | 0.006948 | 0.004604 | 0.004453 | 0.011658 | 0.029126 | 0.001043 | 0.004501 | 0.014522 | 0.010887 | 0.002455 |
Eisosomes | 0.000190 | 0.000570 | 0.000182 | 0.000351 | 0.000154 | 0.000218 | 0.000293 | 0.000179 | 0.000172 | 0.000312 | 0.000201 | 0.000247 |
Endoplasmic Reticulum | 0.000120 | 0.000244 | 0.000047 | 0.000185 | 0.000127 | 0.000207 | 0.000177 | 0.000129 | 0.000124 | 0.000217 | 0.000070 | 0.000151 |
Endosome | 0.001466 | 0.005484 | 0.002048 | 0.001529 | 0.002198 | 0.007222 | 0.008539 | 0.000718 | 0.001827 | 0.006761 | 0.003201 | 0.001040 |
Golgi | 0.006059 | 0.030524 | 0.009110 | 0.018087 | 0.009474 | 0.024087 | 0.027601 | 0.007627 | 0.007742 | 0.025795 | 0.012394 | 0.011774 |
Lipid Particles | 0.001798 | 0.008305 | 0.002204 | 0.001325 | 0.002513 | 0.007884 | 0.004289 | 0.000303 | 0.002150 | 0.007996 | 0.002574 | 0.000708 |
Mitochondria | 0.969910* | 0.882177* | 0.955224* | 0.948660* | 0.962409* | 0.917198* | 0.861712* | 0.978071* | 0.966214* | 0.907902* | 0.938615* | 0.966412* |
Mitotic Spindle | 0.001174 | 0.002194 | 0.001744 | 0.001125 | 0.001148 | 0.002230 | 0.011940 | 0.000326 | 0.001161 | 0.002221 | 0.003555 | 0.000643 |
None | 0.001002 | 0.001176 | 0.001248 | 0.001371 | 0.001287 | 0.000902 | 0.001329 | 0.000797 | 0.001142 | 0.000975 | 0.001262 | 0.001025 |
Nuclear Periphery | 0.000098 | 0.000174 | 0.000372 | 0.000203 | 0.000388 | 0.000394 | 0.000755 | 0.000105 | 0.000241 | 0.000336 | 0.000440 | 0.000144 |
Nuclear Periphery Foci | 0.000067 | 0.000449 | 0.000168 | 0.000174 | 0.000420 | 0.000396 | 0.000406 | 0.000081 | 0.000241 | 0.000410 | 0.000210 | 0.000118 |
Nucleolus | 0.000074 | 0.000751 | 0.000435 | 0.000145 | 0.000233 | 0.000457 | 0.000597 | 0.000132 | 0.000152 | 0.000535 | 0.000464 | 0.000138 |
Nucleus | 0.000024 | 0.000138 | 0.000089 | 0.000089 | 0.000078 | 0.000144 | 0.000107 | 0.000011 | 0.000051 | 0.000142 | 0.000092 | 0.000042 |
Peroxisomes | 0.004774 | 0.027372 | 0.004270 | 0.003202 | 0.004851 | 0.006688 | 0.020841 | 0.000677 | 0.004812 | 0.012178 | 0.007213 | 0.001678 |
Vacuole | 0.001395 | 0.004204 | 0.005336 | 0.002743 | 0.003182 | 0.006790 | 0.008229 | 0.001942 | 0.002275 | 0.006104 | 0.005850 | 0.002260 |
Vacuole Periphery | 0.003639 | 0.003096 | 0.007055 | 0.008396 | 0.004251 | 0.005681 | 0.007692 | 0.005098 | 0.003940 | 0.004995 | 0.007169 | 0.006406 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.78 | 1.21 | -4.06 | 0.43 | -5.21 | -3.18 | -4.11 | -1.44 | 2.34 | 3.62 | -3.94 | -1.94 | -3.81 | 1.14 | -2.34 |
Bud Neck | -2.25 | -1.00 | 0.09 | 2.58 | 1.74 | -2.54 | -5.93 | 1.94 | 3.92 | 6.78 | -3.21 | -4.29 | 1.65 | 4.40 | 6.96 |
Bud Site | -1.52 | -0.37 | -0.72 | 1.17 | -0.42 | -2.01 | -2.97 | 1.00 | 3.13 | 3.56 | -2.73 | -1.75 | 0.53 | 3.22 | 2.44 |
Cell Periphery | 0.09 | 1.30 | -0.01 | -0.16 | -2.59 | -1.19 | 0.72 | -1.96 | 0.01 | -3.04 | -1.05 | 1.56 | -1.58 | 0.14 | -3.79 |
Cytoplasm | -5.46 | -2.08 | -2.34 | 1.40 | 0.09 | -2.29 | -2.19 | 2.16 | 4.90 | 2.46 | -4.63 | -2.83 | -0.63 | 3.60 | 2.53 |
Cytoplasmic Foci | -7.10 | -2.70 | -0.09 | 7.10 | 2.72 | -6.83 | -7.50 | 6.37 | 11.37 | 8.64 | -9.82 | -7.04 | 4.52 | 12.42 | 9.88 |
Eisosomes | -4.82 | 0.40 | -4.42 | 2.64 | -5.04 | -1.30 | -4.23 | -0.96 | 0.82 | 3.83 | -3.38 | -1.66 | -3.57 | 1.54 | -2.40 |
Endoplasmic Reticulum | -2.00 | 3.05 | -1.00 | 0.72 | -2.31 | -1.86 | -1.29 | -0.03 | 1.61 | 1.08 | -2.80 | 2.73 | -0.72 | 1.55 | -2.44 |
Endosome | -4.55 | -1.83 | -0.22 | 4.50 | 1.72 | -5.73 | -3.95 | 4.13 | 7.99 | 4.98 | -7.40 | -3.48 | 3.46 | 8.90 | 6.11 |
Golgi | -6.71 | -2.58 | -6.65 | 3.15 | -4.64 | -6.48 | -4.83 | 1.24 | 7.84 | 5.45 | -9.88 | -3.77 | -3.69 | 7.39 | 0.47 |
Lipid Particles | -3.32 | -0.85 | 0.89 | 3.53 | 1.60 | -4.39 | -2.29 | 4.77 | 6.68 | 6.34 | -5.75 | -1.02 | 4.41 | 7.34 | 5.26 |
Mitochondria | 9.29 | 4.06 | 5.32 | -6.88 | 1.55 | 8.34 | 8.50 | -4.52 | -12.66 | -10.05 | 12.84 | 7.59 | -0.07 | -13.23 | -7.97 |
Mitotic Spindle | -0.85 | -0.71 | 0.06 | 1.05 | 1.16 | -1.18 | -3.56 | 1.39 | 2.70 | 3.90 | -1.45 | -2.99 | 1.11 | 2.68 | 4.32 |
None | -1.82 | -3.22 | -4.70 | -2.04 | -1.61 | 4.02 | -0.35 | 5.52 | 1.83 | 5.58 | 2.61 | -1.77 | 2.03 | -0.99 | 4.31 |
Nuclear Periphery | -2.61 | -4.82 | -3.37 | -0.82 | 2.81 | -0.03 | -1.65 | 1.61 | 2.45 | 4.66 | -0.78 | -1.98 | 1.10 | 2.20 | 5.52 |
Nuclear Periphery Foci | -2.88 | -5.29 | -1.04 | 1.63 | -0.07 | 0.10 | 0.06 | 1.53 | 3.91 | 4.49 | -1.35 | 0.28 | 1.04 | 3.71 | 1.85 |
Nucleolus | -2.27 | -4.48 | -1.67 | 2.01 | 3.22 | -1.62 | -1.90 | 0.87 | 2.30 | 2.41 | -3.17 | -3.76 | 0.22 | 3.15 | 3.59 |
Nucleus | -2.33 | -1.75 | -1.04 | 0.45 | -0.06 | -1.37 | -0.87 | 2.82 | 3.19 | 4.16 | -2.60 | -1.30 | 0.16 | 2.21 | 1.16 |
Peroxisomes | -5.61 | 0.50 | 1.55 | 6.01 | 1.14 | -1.48 | -4.69 | 4.43 | 7.33 | 6.14 | -5.42 | -2.43 | 4.76 | 8.38 | 6.58 |
Vacuole | -3.00 | -6.39 | -3.29 | 1.49 | 3.82 | -3.92 | -2.70 | 1.96 | 5.56 | 3.40 | -5.66 | -5.65 | 0.03 | 5.65 | 5.63 |
Vacuole Periphery | 0.81 | -4.20 | -4.82 | -5.80 | -1.31 | -1.82 | -2.23 | -1.06 | 0.70 | 1.66 | -1.83 | -4.75 | -3.99 | -2.18 | 1.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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