Standard name
Description Epsin-like protein required for endocytosis and actin patch assembly; functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus; ENT2 has a paralog, ENT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.22 0.22 0.28 0.2 0.19 0.22 0.22 0.23 0.21 0.21 0.21 0.21 – 0.18 0 0.33 0.27 0.24 0.54 0.55 0.5 0.55 0.54 0.5
Bud 0.07 0.05 0 0.05 0.06 0.07 0.08 0.07 0.1 0.07 0.1 0.06 – 0 0 0.07 0.07 0.07 0 0 0 0 0.06 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.07 0.08 0 0.07 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0.08 0.22 0.14 0.16 0.09 0.11 0.11 0.11 0.05 0.08 0.08 0.14 – 0.11 0.14 0.09 0.07 0.05 0.06 0.1 0.06 0.11 0 0
Cytoplasm 0.6 0.49 0.5 0.53 0.59 0.43 0.39 0.4 0.29 0.38 0.32 0.37 – 0.64 0.74 0.3 0.29 0.3 0.15 0.12 0.14 0.09 0.06 0.08
Endoplasmic Reticulum 0.15 0.3 0.25 0.28 0.16 0.23 0.21 0.22 0.2 0.2 0.23 0.17 – 0.21 0.25 0 0 0.05 0.06 0.08 0.06 0.07 0 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0 0.14 0 0 0.05 0.05 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.11 0.14 0.07 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 0 0.16 0.11 0.25 0.2 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0.05
SpindlePole 0 0 0 0 0.05 0.05 0.07 0.1 0.07 0 0 0 – 0 0 0.22 0.26 0.27 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0.09 0.07 0.13 0.15 0.09 0.12 – 0 0 0.07 0.1 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 75 108 59 30 39 55 64 68 67 49 57 51 – 5 3 55 43 37 229 315 129 17 33 43
Bud 25 26 6 7 13 16 25 22 32 15 26 16 – 1 1 11 11 11 8 9 6 0 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 25 42 21 0 4 4
Bud Site 4 2 0 0 0 1 3 2 2 3 0 1 – 0 0 0 2 1 – – – – – –
Cell Periphery 29 109 30 23 19 26 31 32 16 18 23 34 – 3 10 15 11 7 27 55 14 3 2 3
Cytoplasm 207 243 104 78 122 105 115 118 94 87 88 91 – 18 53 50 46 46 65 72 34 2 3 6
Endoplasmic Reticulum 52 149 53 41 34 56 61 66 64 46 62 42 – 6 18 6 3 7 25 44 15 2 2 6
Endosome 14 0 0 0 1 7 13 10 13 5 5 3 – 0 3 10 6 21 10 8 13 1 3 3
Golgi 1 1 2 0 3 3 4 9 7 5 9 11 – 0 0 19 22 11 4 6 1 0 0 1
Mitochondria 4 1 0 2 0 12 11 5 51 25 69 50 – 0 0 3 2 4 8 6 8 0 1 2
Nucleus 3 1 1 1 0 0 1 2 3 2 1 1 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 2 0 0 0 0 2 2 0 1 1 0 3 – 0 0 1 4 2 0 0 0 0 0 0
Peroxisomes 1 1 0 0 0 3 0 3 2 4 1 2 – 0 0 1 6 8 1 0 3 0 0 4
SpindlePole 2 4 4 0 11 13 20 29 23 7 3 7 – 1 0 37 42 41 3 0 1 0 1 0
Vac/Vac Membrane 5 8 3 0 7 17 27 21 42 34 24 30 – 0 0 12 16 12 11 9 3 0 1 0
Unique Cell Count 345 498 208 148 207 245 295 294 319 229 271 248 28 72 169 159 152 427 577 256 32 63 87
Labelled Cell Count 424 653 262 182 249 316 377 387 417 302 368 342 34 88 220 215 208 427 577 256 32 63 87


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 11.1 9.6 9.2 10.8 10.0 10.0 10.4 8.8 9.4 8.8 9.6 – 11.6 11.3 16.1 16.3 14.9 – – 10.5
Std Deviation (1e-4) 1.3 1.7 1.2 1.3 1.6 1.8 1.7 1.9 1.9 1.8 2.0 2.4 – 1.9 1.5 3.9 3.8 3.3 – – 1.8
Intensity Change (Log2) – – – -0.05 0.17 0.06 0.06 0.12 -0.13 -0.03 -0.12 0.01 – 0.28 0.24 0.75 0.76 0.64 – – 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.7 -2.3 -1.4 -1.7 -1.3 -1.9 -1.7 -1.9 -1.9 – -1.2 -4.3 0.9 -0.3 -0.9
Bud 0.9 1.7 1.8 2.6 2.2 3.1 1.8 2.9 1.8 – 0 0 1.7 1.8 1.9
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0.3 -1.7 -1.2 -1.3 -1.2 -3.7 -2.2 -2.1 -0.2 – 0 -0.1 -1.7 -2.3 -3.0
Cytoplasm 0.5 1.8 -1.5 -2.5 -2.2 -4.8 -2.5 -3.9 -2.9 – 1.4 3.5 -4.0 -4.1 -3.8
Endoplasmic Reticulum 0.5 -2.3 -0.7 -1.3 -0.8 -1.5 -1.3 -0.7 -2.2 – -0.5 -0.1 -5.8 -6.2 -5.2
Endosome 0 0 0 3.1 0 2.9 0 0 0 – 0 0 0 0 5.5
Golgi 0 0 0 0 0 0 0 0 2.2 – 0 0 4.3 4.9 3.2
Mitochondria 0 0 3.2 0 0 6.1 4.9 7.9 6.9 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 1.9 1.9 2.5 3.5 2.7 0.8 0 0.6 – 0 0 6.2 7.0 7.1
Vacuole 0 0 2.8 3.6 2.9 4.7 5.0 3.5 4.4 – 0 0 2.8 3.7 3.0