Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; plays a role in determining prion variants; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.96 0.88 0.92 0.87 0.71 0.72 0.66 0.7 0.67 0.66 0.98 0.98 1.0 0.95 0.96 0.98 0.95 0.92 0.91 0.97 0.95 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.08 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.1 0 0.05 0.13 0.15 0.18 0.2 0.19 0.25 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 12 6 6 7 5 5 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 3 0 1 2 3 0 0 0 0 0 0 0 0 2 0 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 2 7 6 9 15 15 8 13 3 8 0 6 0 8 4 2 0 0 0 0 0 0
Cytoplasm 173 119 174 156 208 167 167 205 162 158 107 121 183 279 241 327 196 212 155 115 172 181 256 258
Endoplasmic Reticulum 0 2 3 4 7 8 15 24 11 13 5 5 2 0 1 13 7 7 1 0 3 1 1 2
Endosome 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 2 0 0 0 1 0 0 1
Golgi 0 0 0 1 4 1 1 4 3 2 2 0 0 0 1 1 1 1 0 0 0 0 0 0
Mitochondria 2 1 0 0 2 2 8 5 17 7 14 4 0 0 0 1 1 2 1 0 2 0 2 0
Nucleus 1 0 0 0 0 1 2 3 7 1 1 2 0 0 0 0 0 0 0 0 2 0 1 1
Nuclear Periphery 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 5 18 2 10 31 44 45 44 30 46 2 4 1 12 6 3 0 2 2 0 0 3
Unique Cell Count 176 122 182 178 227 192 234 285 244 225 160 182 186 284 242 343 205 217 163 125 189 187 270 277
Labelled Cell Count 178 126 184 186 231 202 254 308 260 248 170 192 187 289 244 362 217 227 163 125 189 187 270 277


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.4 35.6 29.3 27.3 27.3 25.5 23.2 24.6 23.1 22.8 21.1 22.9 26.4 34.9 36.8 56.9 58.5 52.2 30.5 34.3 42.8
Std Deviation (1e-4) 5.9 9.8 13.1 11.6 8.3 7.9 8.1 7.6 9.3 8.2 9.2 9.6 6.9 9.5 10.1 25.9 35.3 24.2 6.6 10.0 12.7
Intensity Change (Log2) – – – -0.1 -0.1 -0.2 -0.34 -0.25 -0.34 -0.36 -0.48 -0.36 -0.15 0.25 0.33 0.96 1.0 0.83 0.06 0.23 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.1 2.7 2.3 0 2.5 0 1.9 0 0 0 0 0 0
Cytoplasm -2.7 -1.6 -2.9 -6.4 -6.4 -7.3 -6.6 -6.9 -7.1 1.6 1.7 2.8 -0.1 0 1.2
Endoplasmic Reticulum 0 0 1.4 2.4 3.1 1.6 2.1 0 0 0 0 0 1.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.6 0 4.1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.9 0 1.2 3.8 4.4 5.0 5.2 4.9 6.2 0 0 0 0.5 0.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0261 0.0022 0.0102 0.0218 0.0053 0.003 0.0253 0.0005 0.0095 0.0066 0 0.0034 0.2791 0.0006 0.001 0.0015 0 0.004
Bud 0.0007 0.0039 0.0015 0.0021 0.0005 0.0003 0.002 0.0014 0.0069 0.0002 0 0.0004 0.0006 0.0027 0.0005 0.0006 0 0.0003
Bud Neck 0.0006 0.0002 0.0005 0.0026 0.0006 0.0009 0.0048 0.0002 0.0065 0.0007 0.0002 0.0009 0.0193 0.0001 0.0001 0.0004 0.0002 0.0005
Bud Periphery 0.0004 0.0011 0.0005 0.0014 0.0003 0.0002 0.0017 0.0002 0.0014 0.0002 0 0.0002 0.0012 0.0006 0.0002 0.0005 0 0.0004
Bud Site 0.0031 0.0019 0.0018 0.0099 0.0004 0.0002 0.0127 0.0012 0.0171 0.0003 0 0.0002 0.0015 0.0015 0.0003 0.0016 0 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0.0013 0.0001 0 0.0006 0.0001 0.0002 0 0 0 0.0008 0.0003 0.0003 0.0005 0 0
Cytoplasm 0.766 0.9671 0.9233 0.8321 0.7689 0.9193 0.6442 0.9709 0.8984 0.898 0.9898 0.9225 0.6604 0.9566 0.8341 0.7268 0.9753 0.8112
Cytoplasmic Foci 0.0561 0.0028 0.0062 0.0417 0.0166 0.0057 0.0996 0.0024 0.0096 0.0087 0.0003 0.0089 0.0014 0.0026 0.018 0.0839 0.0001 0.0086
Eisosomes 0.0001 0 0.0001 0.0003 0.0002 0 0.0002 0 0.0001 0 0 0 0.0035 0 0.0008 0 0 0
Endoplasmic Reticulum 0.0034 0.0005 0.0031 0.0018 0.0011 0.004 0.0109 0.0007 0.0011 0.0011 0.0003 0.001 0.0032 0.0003 0.0021 0.0229 0.0003 0.0061
Endosome 0.0349 0.0042 0.0143 0.0193 0.05 0.0232 0.0409 0.0037 0.0144 0.007 0.0003 0.018 0.0052 0.0084 0.0472 0.0568 0 0.0478
Golgi 0.0034 0.0025 0.0071 0.0203 0.0183 0.0043 0.0083 0.0011 0.0077 0.0224 0 0.0023 0.0088 0.0003 0.0485 0.0112 0 0.0088
Lipid Particles 0.0082 0.0008 0.0012 0.0047 0.0099 0.0056 0.0188 0.0003 0.0047 0.0025 0 0.0028 0.0016 0.0001 0.002 0.031 0 0.0008
Mitochondria 0.0064 0.0023 0.0051 0.0199 0.0224 0.0075 0.021 0.0048 0.0027 0.0117 0 0.0025 0.0014 0.0197 0.0203 0.0027 0 0.09
None 0.0094 0.0008 0.0104 0.0012 0.0019 0.0012 0.0524 0.0009 0.0008 0.0004 0.0003 0.0009 0.001 0.0008 0.0113 0.0008 0.0001 0.0005
Nuclear Periphery 0.0103 0.0012 0.0022 0.0016 0.0018 0.0066 0.0131 0.001 0.0018 0.005 0.0007 0.0063 0.0037 0.0005 0.0009 0.0101 0.002 0.0106
Nucleolus 0.0002 0.0001 0.0001 0.0002 0.0031 0.0002 0.0004 0.0007 0.0002 0 0 0.0002 0.0002 0 0.0042 0.0005 0 0
Nucleus 0.0326 0.0069 0.008 0.005 0.0476 0.0126 0.0078 0.0084 0.0109 0.0258 0.0074 0.0101 0.0051 0.0025 0.0042 0.0101 0.0217 0.0046
Peroxisomes 0.0128 0.0004 0.0005 0.0044 0.0429 0.001 0.009 0.0003 0.0034 0.0066 0 0.0025 0.0005 0.0001 0.0016 0.0132 0 0.0008
Punctate Nuclear 0.0093 0.0005 0.0008 0.0024 0.002 0.0018 0.0079 0.0006 0.0007 0.0016 0 0.0116 0.0008 0.0001 0.0002 0.0015 0.0001 0.0004
Vacuole 0.0144 0.0004 0.0022 0.0045 0.0049 0.0016 0.0157 0.0005 0.0017 0.0006 0.0005 0.0038 0.0004 0.0018 0.0009 0.0181 0.0001 0.0015
Vacuole Periphery 0.0013 0.0002 0.0008 0.0014 0.0013 0.001 0.0027 0.0001 0.0004 0.0003 0.0001 0.0012 0.0005 0.0004 0.0011 0.0053 0 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 203.675 227.1494 255.1274 268.3914 210.8743 226.2901 245.9798 297.6594 292.3332 217.2509
Translational Efficiency 2.0712 2.2431 1.9915 2.2032 2.3166 1.8309 2.0674 2.2351 1.933 2.1101

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
30 236 1811 1695 1568 412 2088 1407 1598 648 3899 3102

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2335.59 2565.27 2955.57 6213.22 1937.64 2216.38 3069.49 8889.91 1945.11 2343.44 3016.58 7427.31
Standard Deviation 1372.30 949.79 706.19 2766.98 389.40 582.64 1100.69 4216.64 432.50 756.68 940.03 3744.85
Intensity Change Log 2 — 0.135324 0.339649 1.411554 — 0.193905 0.663698 2.197868 — 0.162182 0.495649 1.821449

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003147 0.000464 0.000091 0.000255 0.000719 0.000342 0.000260 0.000208 0.000765 0.000386 0.000181 0.000234
Bud Neck 0.018978 0.001708 0.001062 0.003435 0.000584 0.001480 0.002692 0.002447 0.000929 0.001563 0.001935 0.002987
Bud Site 0.028731 0.011235 0.001639 0.007029 0.003019 0.003659 0.004029 0.005170 0.003502 0.006418 0.002919 0.006186
Cell Periphery 0.001411 0.000687 0.000248 0.000817 0.000173 0.000339 0.000217 0.000623 0.000196 0.000466 0.000231 0.000729
Cytoplasm 0.112767* 0.803609* 0.819698* 0.621297* 0.893707* 0.775960* 0.590704* 0.668189* 0.879046* 0.786030* 0.697067* 0.642566*
Cytoplasmic Foci 0.027394 0.033843 0.006125 0.009677 0.014020 0.015801 0.003532 0.012888 0.014271 0.022371 0.004736 0.011133
Eisosomes 0.000065 0.000003 0.000001 0.000003 0.000003 0.000002 0.000003 0.000004 0.000005 0.000002 0.000002 0.000004
Endoplasmic Reticulum 0.017399 0.000351 0.000060 0.000066 0.000412 0.000283 0.000435 0.000334 0.000731 0.000308 0.000261 0.000188
Endosome 0.025045 0.001779 0.000227 0.001091 0.000641 0.000907 0.000431 0.003416 0.001099 0.001225 0.000336 0.002146
Golgi 0.070671 0.008558 0.001133 0.003056 0.002529 0.002681 0.000342 0.004240 0.003809 0.004821 0.000709 0.003593
Lipid Particles 0.004396 0.002518 0.001016 0.000958 0.001018 0.001427 0.000428 0.001120 0.001081 0.001824 0.000701 0.001031
Mitochondria 0.009019 0.000389 0.000169 0.000492 0.000105 0.000151 0.000498 0.000782 0.000272 0.000238 0.000345 0.000624
Mitotic Spindle 0.002638 0.011430 0.007008 0.006585 0.003987 0.015596 0.010575 0.009920 0.003961 0.014079 0.008918 0.008098
None 0.005436 0.007740 0.006410 0.015212 0.008570 0.014182 0.018700 0.023974 0.008511 0.011836 0.012992 0.019186
Nuclear Periphery 0.002578 0.000126 0.000382 0.000345 0.000065 0.000208 0.000519 0.000475 0.000113 0.000178 0.000455 0.000404
Nuclear Periphery Foci 0.014295 0.004706 0.002050 0.000840 0.003605 0.006117 0.001279 0.002049 0.003806 0.005603 0.001637 0.001388
Nucleolus 0.041985 0.000217 0.000157 0.000603 0.000079 0.000205 0.000540 0.000315 0.000866 0.000209 0.000362 0.000472
Nucleus 0.429968* 0.091045 0.139702* 0.312773* 0.062244 0.147812* 0.339443* 0.244358* 0.069147 0.127137 0.246668* 0.281741*
Peroxisomes 0.002343 0.000234 0.000015 0.000087 0.000066 0.000056 0.000049 0.000658 0.000109 0.000121 0.000033 0.000346
Vacuole 0.138415 0.011678 0.012421 0.014862 0.004023 0.012419 0.025029 0.016297 0.006546 0.012149 0.019173 0.015513
Vacuole Periphery 0.043319 0.007678 0.000387 0.000517 0.000429 0.000373 0.000295 0.002532 0.001235 0.003034 0.000338 0.001431

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.52 3.08 2.96 2.56 -3.25 0.80 1.09 1.60 5.80 8.25 0.77 1.44 1.57 4.98 2.65
Bud Neck 2.99 3.02 2.86 -1.99 -3.25 -5.55 -14.92 -13.15 -4.92 2.27 -4.55 -11.32 -10.05 -4.69 -0.09
Bud Site 1.50 2.35 2.08 2.37 -5.07 -2.02 -2.76 -2.98 0.18 -0.28 -3.32 -0.06 -2.91 1.95 -3.61
Cell Periphery 1.95 3.10 2.06 0.10 -7.27 -4.19 -5.02 -11.28 -5.49 -9.13 -5.37 -4.30 -12.72 -3.35 -11.26
Cytoplasm -22.03 -24.42 -17.01 12.77 24.69 13.17 60.23 43.77 13.68 -7.48 12.77 42.30 49.59 18.92 9.40
Cytoplasmic Foci -0.71 2.55 2.15 6.33 -4.00 -2.44 7.34 -3.50 0.47 -10.98 -5.17 6.93 -0.74 5.06 -11.16
Eisosomes 4.21 4.42 4.19 -0.38 -10.04 0.22 -1.00 -0.65 -3.56 1.53 0.53 0.42 -0.07 -2.73 -3.59
Endoplasmic Reticulum 1.90 1.94 1.94 1.57 3.41 0.58 0.22 2.08 2.29 3.52 1.64 2.22 3.10 3.04 4.43
Endosome 2.21 2.34 2.34 2.19 -0.11 -1.43 0.87 -0.50 0.49 -1.00 -0.48 2.49 1.61 2.26 -0.86
Golgi 1.45 1.66 1.67 2.07 0.18 0.03 7.08 2.67 1.90 -1.89 -0.62 4.18 3.21 2.65 -1.09
Lipid Particles 0.22 1.88 1.94 2.29 0.24 -1.86 -0.01 -3.36 -0.78 -4.40 -3.15 -1.11 -3.02 1.49 -2.98
Mitochondria 1.83 1.87 1.82 -0.29 -4.27 -2.60 -17.12 -4.09 -3.32 1.92 0.06 -2.45 -2.18 -2.22 -0.00
Mitotic Spindle -1.85 -5.07 -3.86 0.56 0.78 -2.86 -5.86 -3.76 1.27 2.49 -3.09 -6.14 -3.80 1.48 2.61
None -1.17 -0.60 -3.09 -3.36 -6.22 -1.50 -4.44 -3.27 -0.29 0.77 -1.10 -1.81 -3.39 -0.99 -2.08
Nuclear Periphery 2.81 2.19 2.26 -6.59 0.32 -6.72 -21.67 -17.71 -10.24 2.69 -5.31 -13.59 -16.45 -11.02 2.05
Nuclear Periphery Foci 1.82 3.01 3.51 4.36 5.82 -2.09 5.04 4.26 3.76 -0.43 -2.51 4.30 6.10 5.56 3.87
Nucleolus 2.23 2.23 2.23 -1.25 -1.47 -3.30 -5.31 -7.37 -1.55 2.84 1.69 1.10 1.35 -1.92 1.26
Nucleus 5.85 4.77 1.92 -21.96 -23.56 -11.65 -55.14 -36.43 -13.00 11.89 -10.30 -44.75 -47.85 -23.94 -6.34
Peroxisomes 2.62 2.99 2.96 1.55 -1.74 0.10 0.75 -0.77 -0.79 -0.91 -0.42 2.65 0.06 0.34 -1.06
Vacuole 3.16 2.90 2.61 -5.20 -3.75 -5.86 -19.55 -19.11 -9.83 -3.61 -6.38 -20.24 -22.65 -10.29 -4.23
Vacuole Periphery 2.13 2.77 2.78 1.40 1.60 0.62 1.52 -0.61 -0.84 -1.11 -0.92 2.70 1.88 1.31 -0.72
Standard name
Human Ortholog
Description Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; plays a role in determining prion variants; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Cpr6

Cpr6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cpr6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available