Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 123 | 48 | 106 | 117 | 138 | 113 | 142 | 98 | 56 | 56 | 46 | 72 | 97 | 79 | 83 | 7 | 9 | 5 | 53 | 34 | 62 | 18 | 24 | 46 |
Bud | 27 | 22 | 44 | 31 | 44 | 43 | 56 | 17 | 19 | 17 | 21 | 16 | 19 | 11 | 23 | 1 | 4 | 0 | 9 | 2 | 2 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 22 | 25 | 23 | 4 | 4 | 6 |
Bud Site | 11 | 2 | 2 | 3 | 4 | 2 | 3 | 4 | 0 | 3 | 0 | 1 | 3 | 3 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 104 | 39 | 145 | 109 | 82 | 104 | 113 | 80 | 41 | 62 | 38 | 60 | 125 | 130 | 144 | 11 | 18 | 10 | 131 | 56 | 203 | 89 | 133 | 150 |
Cytoplasm | 32 | 29 | 45 | 41 | 48 | 51 | 59 | 47 | 33 | 26 | 18 | 42 | 37 | 36 | 30 | 3 | 3 | 3 | 4 | 5 | 3 | 2 | 5 | 4 |
Endoplasmic Reticulum | 8 | 6 | 3 | 1 | 11 | 12 | 21 | 21 | 8 | 4 | 13 | 10 | 1 | 1 | 12 | 1 | 0 | 1 | 7 | 11 | 8 | 0 | 4 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 0 | 0 | 0 | 0 | 2 | 4 | 4 | 0 | 1 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 1 |
Mitochondria | 27 | 16 | 6 | 28 | 12 | 93 | 109 | 51 | 99 | 119 | 109 | 96 | 14 | 14 | 15 | 0 | 4 | 4 | 1 | 8 | 17 | 3 | 3 | 2 |
Nucleus | 2 | 2 | 6 | 0 | 0 | 0 | 2 | 4 | 2 | 2 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 1 | 1 | 0 | 1 | 1 | 21 | 1 | 5 | 1 | 1 | 0 | 3 | 13 | 31 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
SpindlePole | 5 | 0 | 11 | 25 | 21 | 6 | 3 | 12 | 0 | 1 | 0 | 0 | 2 | 8 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 1 | 1 | 1 | 2 | 0 | 11 | 0 | 15 | 0 | 5 | 0 | 4 | 1 | 3 | 0 | 0 | 0 | 1 | 3 | 8 | 7 | 1 | 0 | 1 |
Unique Cell Count | 205 | 114 | 254 | 217 | 236 | 280 | 308 | 233 | 165 | 175 | 162 | 182 | 195 | 199 | 213 | 17 | 25 | 18 | 242 | 168 | 340 | 127 | 181 | 225 |
Labelled Cell Count | 341 | 167 | 371 | 358 | 361 | 460 | 510 | 355 | 259 | 297 | 249 | 306 | 314 | 321 | 321 | 25 | 38 | 25 | 242 | 168 | 340 | 127 | 181 | 225 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 6.5 | 6.8 | 6.1 | 6.9 | 6.0 | 6.1 | 6.9 | 5.4 | 5.5 | 5.3 | 6.0 | 7.1 | 7.3 | 7.3 | 13.0 | 12.2 | 13.2 | 6.5 | 6.7 | 7.2 |
Std Deviation (1e-4) | 1.3 | 1.1 | 0.9 | 0.8 | 0.9 | 1.1 | 0.9 | 1.3 | 0.7 | 0.8 | 0.9 | 0.9 | 2.2 | 1.6 | 1.4 | 3.6 | 2.0 | 3.4 | 1.9 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.17 | 0.01 | -0.19 | -0.15 | 0.01 | -0.33 | -0.3 | -0.36 | -0.19 | 0.06 | 0.1 | 0.1 | 0.93 | 0.84 | 0.95 | -0.07 | -0.02 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.6 | 3.7 | -0.3 | 1.0 | 0.1 | -1.6 | -2.0 | -2.8 | -0.5 | 1.7 | -0.4 | -0.6 | 0 | -0.6 | -1.2 |
Bud | -0.9 | 0.4 | -0.6 | 0.3 | -3.3 | -1.6 | -2.2 | -1.2 | -2.6 | -2.3 | -3.8 | -2.0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.5 | -5.0 | -4.6 | -4.8 | -5.0 | -6.5 | -4.4 | -6.7 | -5.0 | 1.5 | 1.8 | 2.3 | 0.6 | 1.4 | -0.1 |
Cytoplasm | 0.3 | 0.7 | 0.1 | 0.4 | 0.7 | 0.6 | -0.8 | -1.8 | 1.4 | 0.3 | 0.1 | -1.1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 2.3 | 2.2 | 3.3 | 4.0 | 0 | 0 | 3.5 | 2.6 | 0 | 0 | 2.7 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.4 | 1.6 | 9.2 | 9.7 | 6.7 | 13.3 | 14.7 | 14.4 | 12.3 | 2.5 | 2.4 | 2.4 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 6.5 | 3.7 | 0 | 0 | 0 |
SpindlePole | 2.9 | 2.0 | -1.4 | -2.5 | 0.4 | 0 | 0 | 0 | 0 | -2.1 | -0.2 | -2.6 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 2.8 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.873 | 2.8318 | 3.2151 | 2.6253 | 2.6256 | 3.2159 | 5.182 | 3.9954 | 3.9374 | 3.7819 | 4.0197 | 4.2342 | 4.3173 | 2.8388 | 3.2491 | 3.7214 | 2.7519 | 3.5042 |
Actin | 0.0453 | 0.0346 | 0.0373 | 0.0427 | 0.0641 | 0.0271 | 0.0239 | 0.0023 | 0.0496 | 0.0294 | 0.0237 | 0.0095 | 0.05 | 0.0075 | 0.0259 | 0.0399 | 0.0463 | 0.0143 |
Bud | 0.0048 | 0.0125 | 0.0431 | 0.0478 | 0.0319 | 0.0056 | 0.003 | 0.0034 | 0.0249 | 0.0127 | 0.0171 | 0.0018 | 0.0025 | 0.0056 | 0.0338 | 0.0184 | 0.0213 | 0.0026 |
Bud Neck | 0.0119 | 0.0093 | 0.0265 | 0.008 | 0.0132 | 0.1098 | 0.0061 | 0.0067 | 0.0183 | 0.0091 | 0.039 | 0.0507 | 0.027 | 0.0109 | 0.0227 | 0.0101 | 0.0024 | 0.0639 |
Bud Periphery | 0.0435 | 0.0615 | 0.3139 | 0.6195 | 0.3704 | 0.1087 | 0.025 | 0.0154 | 0.2088 | 0.2605 | 0.3131 | 0.0441 | 0.017 | 0.0354 | 0.3216 | 0.3353 | 0.3836 | 0.0506 |
Bud Site | 0.0456 | 0.4218 | 0.1711 | 0.0205 | 0.0458 | 0.0123 | 0.0211 | 0.3346 | 0.1212 | 0.0115 | 0.0109 | 0.0033 | 0.0567 | 0.4266 | 0.1374 | 0.0335 | 0.0062 | 0.0044 |
Cell Periphery | 0.6272 | 0.3228 | 0.2978 | 0.13 | 0.3293 | 0.6363 | 0.7627 | 0.5348 | 0.4907 | 0.4974 | 0.5513 | 0.8198 | 0.6236 | 0.3846 | 0.3434 | 0.3656 | 0.3126 | 0.745 |
Cytoplasm | 0.0659 | 0.1033 | 0.0522 | 0.0352 | 0.0132 | 0.0577 | 0.0539 | 0.0914 | 0.033 | 0.0333 | 0.0351 | 0.0402 | 0.0645 | 0.1171 | 0.049 | 0.0687 | 0.0567 | 0.0664 |
Cytoplasmic Foci | 0.0148 | 0.0018 | 0.004 | 0.0064 | 0.0283 | 0.002 | 0.0084 | 0.0002 | 0.0046 | 0.0046 | 0.0002 | 0.0003 | 0.0253 | 0.001 | 0.0045 | 0.0008 | 0.0311 | 0.0015 |
Eisosomes | 0.0185 | 0.0097 | 0.0052 | 0.0048 | 0.0022 | 0.0154 | 0.0324 | 0.0056 | 0.0077 | 0.017 | 0.0028 | 0.0154 | 0.0041 | 0.0022 | 0.0053 | 0.0077 | 0.001 | 0.0014 |
Endoplasmic Reticulum | 0.0137 | 0.0041 | 0.0036 | 0.0015 | 0.0032 | 0.0052 | 0.0086 | 0.0014 | 0.0061 | 0.0175 | 0.0025 | 0.0043 | 0.0068 | 0.0021 | 0.0092 | 0.0037 | 0.0018 | 0.0124 |
Endosome | 0.0244 | 0.0006 | 0.0067 | 0.0033 | 0.0317 | 0.0034 | 0.0096 | 0 | 0.0057 | 0.009 | 0.0001 | 0.001 | 0.0376 | 0.0002 | 0.0164 | 0.002 | 0.0458 | 0.006 |
Golgi | 0.0137 | 0.003 | 0.0118 | 0.0086 | 0.0174 | 0.0017 | 0.0048 | 0.0001 | 0.0041 | 0.0129 | 0.0003 | 0.0016 | 0.0125 | 0.0005 | 0.0046 | 0.0081 | 0.0228 | 0.0053 |
Lipid Particles | 0.0121 | 0.0033 | 0.0023 | 0.002 | 0.0096 | 0.001 | 0.0052 | 0.0002 | 0.0024 | 0.01 | 0.0002 | 0.0005 | 0.0176 | 0.0007 | 0.0014 | 0.0226 | 0.0023 | 0.0007 |
Mitochondria | 0.0131 | 0.003 | 0.0074 | 0.0576 | 0.0055 | 0.002 | 0.0061 | 0 | 0.009 | 0.0503 | 0.0002 | 0.0014 | 0.0118 | 0.0001 | 0.0062 | 0.0237 | 0.012 | 0.0055 |
None | 0.0034 | 0.003 | 0.0089 | 0.0009 | 0.0016 | 0.0024 | 0.0025 | 0.0004 | 0.0014 | 0.0063 | 0.0004 | 0.0004 | 0.0142 | 0.0011 | 0.002 | 0.0461 | 0.0005 | 0.0014 |
Nuclear Periphery | 0.0092 | 0.0005 | 0.0004 | 0.0003 | 0.007 | 0.0005 | 0.0017 | 0.0003 | 0.0017 | 0.0038 | 0.0003 | 0.0006 | 0.0013 | 0.0004 | 0.0027 | 0.0009 | 0.0003 | 0.0095 |
Nucleolus | 0.001 | 0.0003 | 0.0004 | 0.0002 | 0.0013 | 0.0005 | 0.001 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0003 |
Nucleus | 0.0033 | 0.0006 | 0.0007 | 0.0004 | 0.0032 | 0.001 | 0.0012 | 0.0004 | 0.0013 | 0.0018 | 0.0002 | 0.0015 | 0.0012 | 0.0005 | 0.0012 | 0.0029 | 0.0003 | 0.002 |
Peroxisomes | 0.0126 | 0.0006 | 0.0012 | 0.0078 | 0.0087 | 0.0009 | 0.0074 | 0 | 0.0033 | 0.0039 | 0 | 0.0001 | 0.0133 | 0.0001 | 0.0002 | 0.0018 | 0.0469 | 0.0002 |
Punctate Nuclear | 0.0056 | 0.0001 | 0.0001 | 0.0002 | 0.0041 | 0.0002 | 0.0073 | 0 | 0.0018 | 0.0015 | 0 | 0.0001 | 0.0023 | 0.0001 | 0.0004 | 0.0004 | 0.002 | 0.0002 |
Vacuole | 0.0085 | 0.0034 | 0.0051 | 0.0019 | 0.0071 | 0.006 | 0.0074 | 0.0026 | 0.0038 | 0.0062 | 0.0026 | 0.0031 | 0.0093 | 0.0031 | 0.0101 | 0.0064 | 0.0027 | 0.0054 |
Vacuole Periphery | 0.002 | 0.0002 | 0.0004 | 0.0004 | 0.0013 | 0.0004 | 0.0006 | 0 | 0.0003 | 0.0008 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0018 | 0.001 | 0.0012 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.6313 | 13.9816 | 13.0523 | 13.5076 | 17.0077 | 12.102 | 20.2931 | 19.0827 | 17.9137 | 17.5961 |
Translational Efficiency | 0.9789 | 0.8968 | 0.762 | 0.7278 | 0.6259 | 1.1962 | 0.7519 | 0.7685 | 0.8728 | 0.9125 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
382 | 955 | 1134 | 1185 | 1482 | 1835 | 234 | 46 | 1864 | 2790 | 1368 | 1231 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 775.77 | 940.85 | 1201.67 | 1046.23 | 792.07 | 838.81 | 1216.46 | 1413.29 | 788.73 | 873.74 | 1204.20 | 1059.95 |
Standard Deviation | 99.69 | 136.06 | 162.10 | 128.88 | 104.16 | 121.43 | 165.19 | 209.68 | 103.47 | 135.57 | 162.73 | 149.93 |
Intensity Change Log 2 | — | 0.278336 | 0.631340 | 0.431499 | — | 0.082716 | 0.618989 | 0.835358 | — | 0.182823 | 0.625113 | 0.649598 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.064032 | 0.102669* | 0.083281 | 0.057366 | 0.069963 | 0.076271 | 0.091574 | 0.145098* | 0.068748 | 0.085307 | 0.084700 | 0.060645 |
Bud Neck | 0.030543 | 0.051663 | 0.074287 | 0.053521 | 0.043119 | 0.057439 | 0.086075 | 0.071874 | 0.040542 | 0.055462 | 0.076304 | 0.054207 |
Bud Site | 0.039528 | 0.137093* | 0.203109* | 0.177207* | 0.057538 | 0.088301 | 0.186728* | 0.194477* | 0.053847 | 0.105002* | 0.200307* | 0.177852* |
Cell Periphery | 0.666167* | 0.519432* | 0.453101* | 0.614307* | 0.445877* | 0.412447* | 0.418939* | 0.255318* | 0.491022* | 0.449068* | 0.447257* | 0.600892* |
Cytoplasm | 0.051486 | 0.089682 | 0.053160 | 0.057885 | 0.158977* | 0.163410* | 0.028108 | 0.234199* | 0.136948* | 0.138173* | 0.048875 | 0.064474 |
Cytoplasmic Foci | 0.004114 | 0.004348 | 0.002361 | 0.001592 | 0.005708 | 0.006565 | 0.005605 | 0.001837 | 0.005381 | 0.005806 | 0.002916 | 0.001601 |
Eisosomes | 0.070852 | 0.055465 | 0.032169 | 0.022054 | 0.129465* | 0.103237* | 0.059200 | 0.052247 | 0.117453* | 0.086885 | 0.036793 | 0.023183 |
Endoplasmic Reticulum | 0.005174 | 0.006237 | 0.045473 | 0.003944 | 0.007694 | 0.008659 | 0.039050 | 0.003702 | 0.007178 | 0.007830 | 0.044374 | 0.003935 |
Endosome | 0.003236 | 0.001387 | 0.001872 | 0.000589 | 0.002100 | 0.003086 | 0.001867 | 0.000239 | 0.002333 | 0.002505 | 0.001871 | 0.000576 |
Golgi | 0.002301 | 0.002575 | 0.001944 | 0.000610 | 0.006396 | 0.006194 | 0.001405 | 0.000150 | 0.005557 | 0.004955 | 0.001852 | 0.000593 |
Lipid Particles | 0.002492 | 0.001919 | 0.007429 | 0.000970 | 0.005517 | 0.005686 | 0.003649 | 0.000482 | 0.004897 | 0.004397 | 0.006782 | 0.000952 |
Mitochondria | 0.015261 | 0.008174 | 0.000580 | 0.000487 | 0.006127 | 0.009521 | 0.000154 | 0.003623 | 0.007999 | 0.009060 | 0.000507 | 0.000605 |
Mitotic Spindle | 0.000273 | 0.000794 | 0.000362 | 0.000195 | 0.000121 | 0.000155 | 0.000174 | 0.000034 | 0.000152 | 0.000374 | 0.000330 | 0.000189 |
None | 0.000102 | 0.000073 | 0.000080 | 0.000042 | 0.000173 | 0.000168 | 0.000024 | 0.000073 | 0.000158 | 0.000135 | 0.000070 | 0.000043 |
Nuclear Periphery | 0.000044 | 0.000058 | 0.000289 | 0.000094 | 0.000090 | 0.000076 | 0.000134 | 0.000035 | 0.000081 | 0.000070 | 0.000263 | 0.000091 |
Nuclear Periphery Foci | 0.000106 | 0.000139 | 0.001117 | 0.000212 | 0.000153 | 0.000151 | 0.002335 | 0.000033 | 0.000143 | 0.000147 | 0.001325 | 0.000205 |
Nucleolus | 0.000028 | 0.000030 | 0.000022 | 0.000013 | 0.000028 | 0.000037 | 0.000013 | 0.000032 | 0.000028 | 0.000034 | 0.000021 | 0.000014 |
Nucleus | 0.000923 | 0.001969 | 0.006857 | 0.000977 | 0.003608 | 0.002276 | 0.006163 | 0.014323 | 0.003058 | 0.002171 | 0.006738 | 0.001476 |
Peroxisomes | 0.039988 | 0.010187 | 0.001381 | 0.000622 | 0.053583 | 0.052239 | 0.000431 | 0.001418 | 0.050797 | 0.037845 | 0.001218 | 0.000651 |
Vacuole | 0.001916 | 0.005701 | 0.030826 | 0.007202 | 0.003531 | 0.003366 | 0.068319 | 0.020740 | 0.003200 | 0.004165 | 0.037239 | 0.007708 |
Vacuole Periphery | 0.001434 | 0.000405 | 0.000300 | 0.000109 | 0.000232 | 0.000716 | 0.000051 | 0.000065 | 0.000478 | 0.000609 | 0.000258 | 0.000108 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.04 | -2.20 | 0.78 | 6.74 | 4.77 | -1.29 | -2.13 | -2.43 | -2.23 | -1.71 | -3.78 | -3.17 | 1.66 | 5.30 | 4.59 |
Bud Neck | -4.06 | -8.57 | -4.66 | -0.42 | 4.73 | -4.06 | -5.15 | -1.80 | -0.88 | 0.77 | -5.12 | -9.89 | -3.86 | 0.36 | 5.25 |
Bud Site | -11.36 | -19.31 | -16.18 | -4.20 | 2.66 | -5.96 | -8.61 | -3.85 | -2.98 | -0.21 | -11.28 | -21.83 | -17.23 | -9.75 | 2.42 |
Cell Periphery | 7.29 | 11.44 | 2.72 | -6.52 | -12.87 | 2.99 | 1.74 | 4.15 | 3.45 | 3.21 | 4.22 | 4.38 | -8.96 | -13.44 | -12.88 |
Cytoplasm | -5.89 | -0.50 | -1.05 | 6.29 | -0.78 | -0.70 | 21.82 | -2.11 | -1.99 | -5.67 | -0.25 | 18.14 | 13.62 | 15.72 | -3.35 |
Cytoplasmic Foci | -0.26 | 1.35 | 1.78 | 2.33 | 0.93 | -0.99 | -0.47 | 2.37 | 2.89 | 2.10 | -0.63 | 3.31 | 4.57 | 5.10 | 1.85 |
Eisosomes | 2.08 | 5.63 | 7.08 | 9.62 | 4.69 | 4.93 | 10.11 | 4.42 | 2.95 | 0.34 | 6.98 | 20.49 | 23.99 | 21.75 | 5.92 |
Endoplasmic Reticulum | -0.75 | -13.39 | 1.06 | 2.16 | 14.51 | -0.75 | -6.72 | 1.49 | 1.81 | 6.72 | -0.65 | -14.54 | 3.93 | 4.51 | 16.10 |
Endosome | 2.61 | 1.96 | 3.81 | 3.19 | 6.18 | -1.79 | 0.11 | 5.95 | 5.99 | 5.16 | -0.42 | 1.38 | 5.62 | 5.56 | 6.74 |
Golgi | -0.39 | 0.74 | 3.11 | 3.52 | 4.07 | 0.19 | 7.17 | 9.18 | 7.21 | 7.22 | 0.76 | 6.52 | 8.18 | 6.91 | 4.14 |
Lipid Particles | 0.52 | -4.95 | 1.93 | 1.15 | 9.10 | -0.17 | 1.74 | 7.11 | 7.24 | 5.20 | 0.65 | -2.42 | 6.46 | 5.96 | 9.63 |
Mitochondria | 1.60 | 3.59 | 3.61 | 4.47 | 1.11 | -2.99 | 10.32 | 2.05 | 4.05 | -3.16 | -0.82 | 7.80 | 7.69 | 9.69 | -1.01 |
Mitotic Spindle | -0.89 | -0.52 | 0.44 | 1.06 | 6.94 | -1.53 | -3.38 | 10.33 | 5.40 | 9.13 | -1.14 | -4.58 | -1.11 | 0.94 | 6.65 |
None | 1.25 | 0.81 | 2.89 | 3.32 | 4.02 | 0.23 | 7.19 | 3.76 | 4.22 | -2.68 | 1.20 | 4.58 | 6.83 | 9.02 | 3.46 |
Nuclear Periphery | -2.30 | -13.49 | -4.16 | -2.83 | 9.22 | 2.17 | -3.72 | 4.72 | 3.79 | 6.67 | 2.04 | -11.64 | -0.90 | -1.89 | 9.23 |
Nuclear Periphery Foci | -1.13 | -13.75 | -3.50 | -3.55 | 12.86 | 0.05 | -5.08 | 5.32 | 4.60 | 5.33 | -0.17 | -12.00 | -2.72 | -2.62 | 11.45 |
Nucleolus | -0.09 | 0.56 | 1.42 | 1.56 | 6.53 | -1.76 | 3.84 | -0.58 | 0.61 | -2.48 | -1.15 | 2.30 | 4.95 | 4.29 | 5.38 |
Nucleus | -2.65 | -5.42 | -0.24 | 3.98 | 5.50 | 4.24 | -2.98 | -1.34 | -1.49 | -0.98 | 3.36 | -4.41 | 3.66 | 1.74 | 5.58 |
Peroxisomes | 5.09 | 6.77 | 6.90 | 7.27 | 4.89 | 0.32 | 17.27 | 16.82 | 18.28 | -2.53 | 3.90 | 18.26 | 18.48 | 19.53 | 4.23 |
Vacuole | -3.73 | -14.91 | -5.72 | -1.27 | 11.88 | 0.27 | -7.31 | -2.27 | -2.29 | 4.28 | -1.81 | -15.10 | -5.15 | -4.15 | 12.70 |
Vacuole Periphery | 1.50 | 1.68 | 1.97 | 2.09 | 2.03 | -2.35 | 7.34 | 5.89 | 3.17 | -0.77 | -0.66 | 1.40 | 2.62 | 3.46 | 1.90 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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