Standard name
Human Ortholog
Description Translation elongation factor 3; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.17 0.11 0 0 0 0 0 0 0 – 0 0.11 0.11 0.05 0 0.25 0.28 0.35 0 0 0 0 0 0
Cytoplasm 0.95 0.91 0.97 1.0 1.0 1.0 0.96 0.9 0.94 – 0.88 0.83 0.97 0.97 0.99 0.87 0.84 0.79 0.96 0.95 0.94 0.89 0.92 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.07 0 – 0.06 0.1 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 1 1 – 1 2 0 0 1 1 0 0 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 19 16 1 2 2 1 5 11 3 – 5 18 15 13 5 117 57 139 2 0 0 0 0 2
Cytoplasm 106 132 76 121 217 162 243 234 121 – 103 134 135 267 291 402 172 316 109 147 88 105 260 320
Endoplasmic Reticulum 0 0 1 0 0 0 6 19 4 – 7 16 0 0 1 11 9 30 0 1 0 1 4 11
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleus 0 3 0 0 1 0 0 0 0 – 0 0 0 0 1 0 0 1 0 0 0 1 1 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 0 0 0 0 2 3 2
Unique Cell Count 112 145 78 121 217 162 252 260 129 117 162 139 274 294 464 204 402 114 155 95 119 283 352
Labelled Cell Count 125 153 78 123 220 164 254 265 129 119 171 150 280 299 535 238 486 114 155 95 119 283 352


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 491.5 448.9 333.0 348.8 313.8 245.6 220.8 197.9 177.5 – 166.0 159.4 477.6 455.0 375.2 488.2 484.5 543.3 425.5 433.9 458.5
Std Deviation (1e-4) 84.1 100.5 88.7 85.5 62.7 60.0 52.1 50.7 53.0 – 54.2 45.5 106.4 87.1 91.9 121.8 154.6 117.6 144.1 130.2 126.7
Intensity Change (Log2) – – – 0.07 -0.09 -0.44 -0.59 -0.75 -0.91 – -1.0 -1.06 0.52 0.45 0.17 0.55 0.54 0.71 0.35 0.38 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 2.6 2.6 0 0 4.7 5.0 5.9
Cytoplasm 1.8 2.4 2.0 -0.4 -2.1 -1.2 – -2.3 -3.2 -0.1 0 1.1 -2.7 -3.0 -3.9
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 2.4 0 0 0 0 0 2.0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 284.5818 530.0769 390.9711 383.4574 338.6926 293.5772 380.7081 698.9622 589.9822 409.236 735.6133 598.8029 434.5529 540.0223 351.9235 375.8712 260.6334 432.4719
Actin 0.0145 0.0007 0.0011 0.0011 0.0036 0.0065 0.0092 0.0007 0.0022 0.0012 0.0006 0.0025 0.003 0.0007 0.0025 0.0634 0.0256 0.0043
Bud 0.0121 0.0044 0.0082 0.0059 0.0085 0.0071 0.0027 0.0037 0.0117 0.0061 0.0044 0.0077 0.0067 0.0052 0.0156 0.0082 0.0091 0.0107
Bud Neck 0.0016 0.001 0.0013 0.0028 0.0032 0.0043 0.0012 0.0008 0.0013 0.0012 0.0011 0.0018 0.0012 0.0007 0.0014 0.0029 0.0025 0.0026
Bud Periphery 0.017 0.0019 0.0049 0.0034 0.0095 0.0066 0.0029 0.0013 0.0056 0.0045 0.001 0.0015 0.0049 0.0018 0.0156 0.0093 0.0054 0.0093
Bud Site 0.0051 0.0025 0.0038 0.0083 0.0036 0.0026 0.0028 0.0024 0.0043 0.0009 0.0002 0.0018 0.0025 0.0026 0.0037 0.0026 0.0046 0.0014
Cell Periphery 0.059 0.0613 0.088 0.0973 0.0433 0.0989 0.0746 0.0306 0.0258 0.0178 0.0064 0.0057 0.0677 0.0146 0.0322 0.0206 0.0068 0.0235
Cytoplasm 0.3107 0.7346 0.4591 0.4886 0.1624 0.3045 0.3388 0.8216 0.6961 0.4427 0.648 0.5915 0.4984 0.8582 0.5095 0.3229 0.4127 0.4627
Cytoplasmic Foci 0.0271 0.0008 0.006 0.0037 0.0061 0.0219 0.0174 0.001 0.0044 0.001 0.0004 0.0036 0.001 0.0008 0.1303 0.0007 0.0144 0.0008
Eisosomes 0.0064 0.0002 0.0014 0.0003 0.0005 0.001 0.0171 0.0046 0.0009 0 0 0.0003 0.0083 0 0.0019 0.0057 0.0012 0.0001
Endoplasmic Reticulum 0.0251 0.0014 0.0029 0.0016 0.0007 0.0206 0.0052 0.0006 0.0011 0.0011 0.0003 0.0003 0.0119 0.0007 0.0023 0.0024 0.056 0.0021
Endosome 0.0052 0.0004 0.0039 0.0088 0.0075 0.107 0.0054 0.0001 0.0011 0.0068 0.0004 0.0008 0.0019 0.0005 0.0059 0.0074 0.1446 0.0019
Golgi 0.0013 0.0001 0.0003 0.0009 0.0019 0.0055 0.0022 0.0001 0.0003 0.0002 0 0.0002 0.0004 0.0001 0.0008 0.0155 0.081 0.0003
Lipid Particles 0.0041 0.0008 0.0026 0.0059 0.0091 0.0137 0.0134 0.0005 0.0018 0.0007 0 0.0005 0.0015 0.0002 0.0067 0.0024 0.0191 0.0006
Mitochondria 0.0702 0.0132 0.0388 0.0057 0.2995 0.0383 0.0547 0.0112 0.037 0.0081 0.0016 0.0944 0.0947 0.0058 0.0507 0.1345 0.0406 0.12
None 0.0356 0.0025 0.0264 0.0036 0.0027 0.0425 0.0405 0.0038 0.0064 0.0016 0.0003 0.0051 0.0063 0.0015 0.04 0.0011 0.0017 0.0013
Nuclear Periphery 0.0886 0.0104 0.0171 0.0328 0.0101 0.0789 0.1166 0.0103 0.0163 0.0352 0.0026 0.0183 0.0556 0.0083 0.0105 0.0099 0.0181 0.0157
Nucleolus 0.037 0.0108 0.0406 0.0321 0.0481 0.0302 0.0542 0.0113 0.0204 0.0154 0.0009 0.0387 0.0105 0.0018 0.0468 0.0039 0.0027 0.0092
Nucleus 0.0797 0.056 0.0656 0.0613 0.0552 0.0465 0.1431 0.0603 0.0767 0.1186 0.0268 0.1746 0.0717 0.0374 0.0332 0.0906 0.0384 0.0431
Peroxisomes 0.0022 0.0005 0.0013 0.0005 0.0042 0.0027 0.0024 0.0004 0.0017 0.0004 0.0001 0.003 0.0009 0.0003 0.0039 0.0007 0.0039 0.0013
Punctate Nuclear 0.0013 0.0001 0.0005 0.0003 0.0004 0.0042 0.003 0.0001 0.0003 0.0002 0 0.0005 0.0002 0.0001 0.0015 0.0001 0.0007 0.0002
Vacuole 0.1277 0.0786 0.1663 0.1849 0.1567 0.0978 0.0524 0.028 0.0608 0.1844 0.2384 0.0212 0.0898 0.0449 0.0666 0.2051 0.0638 0.1617
Vacuole Periphery 0.0684 0.0181 0.0599 0.0502 0.1632 0.0588 0.0402 0.0066 0.0236 0.1518 0.0666 0.0261 0.0608 0.0137 0.0184 0.0902 0.047 0.1273

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3181.7468 3635.276 4088.0889 3215.4698 3591.133 2994.2517 2981.1555 2659.0105 2728.8345 2567.3959
Translational Efficiency 1.0346 1.0452 0.9818 1.2487 1.0707 0.923 1.0164 1.0613 0.9376 1.1284

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1122 776 245 882 1218 1140 1472 237 2340 1916 1717 1119

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 23653.18 24739.94 39112.92 29712.71 23297.32 22536.16 38677.23 33920.55 23467.95 23428.71 38739.40 30603.91
Standard Deviation 4855.99 6325.27 6151.58 7723.48 5754.10 5770.34 6444.73 7537.24 5345.30 6098.00 6405.54 7874.39
Intensity Change Log 2 — 0.064808 0.725611 0.329046 — -0.047922 0.731320 0.541996 — 0.009971 0.728447 0.438641

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000056 0.000107 0.000062 0.000252 0.000041 0.000123 0.000125 0.000149 0.000048 0.000116 0.000116 0.000230
Bud Neck 0.000369 0.000747 0.001344 0.001877 0.000216 0.001026 0.000802 0.000837 0.000289 0.000913 0.000879 0.001657
Bud Site 0.001064 0.003715 0.000338 0.005756 0.001736 0.003230 0.001663 0.002115 0.001414 0.003426 0.001474 0.004985
Cell Periphery 0.031566 0.040495 0.204022* 0.064956 0.021569 0.018173 0.153245* 0.163836* 0.026362 0.027214 0.160490* 0.085899
Cytoplasm 0.698132* 0.640591* 0.130207* 0.313737* 0.737518* 0.701706* 0.212559* 0.197547* 0.718633* 0.676954* 0.200808* 0.289129*
Cytoplasmic Foci 0.009447 0.009851 0.004351 0.004332 0.010027 0.017291 0.001003 0.003430 0.009749 0.014278 0.001480 0.004141
Eisosomes 0.000003 0.000003 0.000023 0.000006 0.000002 0.000005 0.000012 0.000007 0.000002 0.000004 0.000013 0.000006
Endoplasmic Reticulum 0.000519 0.000469 0.001853 0.000331 0.000605 0.000247 0.002244 0.000234 0.000564 0.000337 0.002188 0.000311
Endosome 0.000200 0.000422 0.000161 0.000786 0.000312 0.000434 0.000237 0.000256 0.000258 0.000429 0.000226 0.000674
Golgi 0.052031 0.058127 0.031406 0.016122 0.041744 0.043685 0.009480 0.010337 0.046677 0.049534 0.012609 0.014897
Lipid Particles 0.009769 0.022221 0.030113 0.022808 0.008617 0.011626 0.023470 0.034226 0.009170 0.015917 0.024418 0.025227
Mitochondria 0.000142 0.000193 0.000081 0.001584 0.000294 0.000439 0.000107 0.001720 0.000221 0.000340 0.000104 0.001613
Mitotic Spindle 0.000459 0.000994 0.000295 0.000436 0.000460 0.000749 0.000198 0.000238 0.000459 0.000848 0.000212 0.000394
None 0.003154 0.003844 0.000923 0.002062 0.002799 0.003046 0.000316 0.000980 0.002969 0.003369 0.000403 0.001833
Nuclear Periphery 0.001321 0.001247 0.004984 0.000750 0.000757 0.001052 0.002200 0.001014 0.001027 0.001131 0.002597 0.000806
Nuclear Periphery Foci 0.008725 0.013677 0.008316 0.005249 0.007859 0.010159 0.005460 0.004139 0.008274 0.011584 0.005867 0.005014
Nucleolus 0.000093 0.000182 0.000072 0.000241 0.000101 0.000739 0.000077 0.000109 0.000097 0.000513 0.000077 0.000213
Nucleus 0.016837 0.017190 0.010513 0.015523 0.008393 0.021882 0.004706 0.013268 0.012442 0.019982 0.005534 0.015046
Peroxisomes 0.000007 0.000036 0.000016 0.000075 0.000051 0.000045 0.000082 0.000037 0.000030 0.000042 0.000073 0.000067
Vacuole 0.064052 0.116796 0.466326* 0.492630* 0.070469 0.105488 0.545726* 0.532155* 0.067392 0.110068 0.534396* 0.501001*
Vacuole Periphery 0.102053* 0.069091 0.104594* 0.050485 0.086431 0.058855 0.036288 0.033364 0.093922 0.063001 0.046035 0.046859

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.48 -0.43 -2.75 -2.06 -2.70 -3.79 -3.59 -3.21 -1.05 -0.67 -4.46 -3.44 -3.27 -2.07 -1.78
Bud Neck -2.56 -4.35 -8.87 -6.56 -3.92 -5.97 -6.52 -4.79 0.28 -1.00 -6.28 -6.99 -9.95 -5.68 -6.09
Bud Site -3.27 4.15 -4.79 -1.77 -5.50 -2.46 -0.20 -1.61 0.20 -1.39 -4.09 -0.48 -4.77 -1.95 -4.15
Cell Periphery -2.63 -15.58 -5.79 -3.33 13.13 1.61 -31.09* -13.16 -13.54 -0.35 -0.54 -33.76 -12.92 -12.67 15.96
Cytoplasm 3.63 32.53 24.73 18.27 -10.94 3.49 43.85 24.71 22.11 -0.00 4.71 53.38 35.00 29.18 -8.57
Cytoplasmic Foci -0.42 4.15 3.11 3.50 -1.32 -5.13 9.94 4.22 7.97 -3.07 -4.90 12.90 4.71 8.34 -6.36
Eisosomes -0.93 -7.07 -3.97 -3.60 5.50 -3.03 -11.99 -8.09 -1.14 4.85 -3.07 -13.58 -5.84 -2.00 6.41
Endoplasmic Reticulum 0.14 -1.30 0.67 1.81 1.62 3.20 -3.96 3.29 0.47 5.05 1.30 -3.99 1.65 1.15 5.18
Endosome -5.56 1.69 -2.32 -1.29 -2.49 -2.87 1.46 0.60 1.78 -0.07 -5.64 0.77 -2.02 -0.99 -2.16
Golgi -1.55 6.22 15.15 13.56 6.21 -0.50 15.01 12.62 13.63 -0.36 -1.07 20.65 18.75 18.51 -0.85
Lipid Particles -6.11 -6.67 -7.19 0.44 2.86 -2.87 -10.62 -6.02 -5.22 -2.37 -6.40 -12.42 -9.96 -4.85 0.41
Mitochondria -1.49 3.21 -1.43 -1.37 -1.50 -1.26 4.86 -0.93 -0.81 -1.06 -1.68 4.79 -1.61 -1.44 -1.75
Mitotic Spindle -3.39 3.37 0.59 3.32 -1.08 -2.86 3.82 2.60 5.34 -0.68 -4.50 5.93 1.12 4.52 -2.01
None -1.00 6.48 1.65 1.89 -1.67 -0.40 7.22 2.85 2.61 -1.17 -0.85 13.07 2.06 2.26 -2.82
Nuclear Periphery 0.21 -7.69 1.81 5.24 11.94 -3.65 -12.48 -1.82 0.42 6.57 -0.84 -8.36 1.33 4.88 16.16
Nuclear Periphery Foci -4.48 0.31 4.76 8.51 3.50 -2.85 3.56 5.25 8.02 2.76 -5.01 4.52 6.19 10.72 2.33
Nucleolus -1.31 1.20 -5.35 -0.42 -6.86 -1.27 0.98 -0.21 1.26 -3.35 -1.45 1.34 -4.88 0.97 -7.00
Nucleus -0.30 2.68 -3.21 -2.67 -4.99 -7.57 2.17 -2.04 2.13 -2.75 -5.77 5.75 -5.15 -0.77 -8.14
Peroxisomes -3.14 -1.59 -4.50 -1.85 -3.53 0.21 -1.30 0.76 0.53 3.35 -0.83 -2.89 -2.13 -1.21 0.69
Vacuole -7.27 -24.63 -37.88 -29.54 -0.90 -6.10 -57.93* -24.58 -22.03 1.17 -9.46 -66.24* -44.59 -37.81 3.85
Vacuole Periphery 4.95 -0.17 10.02 5.10 6.20 5.47 11.21 9.80 5.34 1.07 7.57 12.37 13.38 6.33 1.43
Standard name
Human Ortholog
Description Translation elongation factor 3; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Yef3

Yef3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yef3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available