ORF
Human Ortholog
Description Protein of unknown function; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.05 0.05 0.08 0.1 0.06 0.09 0 0 0 0.07 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.99 1.0 0.98 0.98 0.94 0.87 0.89 0.88 0.89 0.83 0.99 0.99 1.0 0.99 0.98 0.99 0.95 0.98 0.96 0.94 0.95 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.1 0.07 0.07 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 4 3 4 8 6 9 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 2 1 0 0 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 4 2 11 14 19 17 28 38 19 26 1 2 4 19 7 4 0 0 0 0 0 0
Cytoplasm 229 334 241 256 382 291 338 284 322 324 279 231 215 336 358 264 335 282 215 321 230 152 294 273
Endoplasmic Reticulum 1 0 2 0 6 3 12 32 24 25 24 29 0 1 1 6 12 9 2 0 1 1 2 2
Endosome 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 1 0 0 0 0 0 1 0 1 1 0 0 0 1 0 2 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 3 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 0 0 0 0 2 0 4 1 0 2 2 0 2 6 4 1 1 1 1 3 2
Unique Cell Count 230 335 243 256 390 297 360 328 363 370 315 278 217 338 359 268 342 286 227 329 240 162 309 289
Labelled Cell Count 234 337 249 258 400 309 373 339 379 402 336 298 218 341 363 292 360 301 227 329 240 162 309 289


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 57.0 92.2 60.0 53.1 52.1 48.1 47.6 47.6 44.1 42.8 38.4 41.4 47.8 48.0 47.3 76.9 85.1 83.1 60.6 60.9 66.0
Std Deviation (1e-4) 11.4 15.1 11.1 10.8 9.4 10.5 11.5 10.2 10.3 10.8 9.8 10.6 10.3 10.0 9.1 25.1 26.3 23.9 16.2 13.9 16.5
Intensity Change (Log2) – – – -0.18 -0.2 -0.32 -0.33 -0.33 -0.44 -0.49 -0.65 -0.54 -0.33 -0.32 -0.34 0.36 0.5 0.47 0.01 0.02 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.9 2.0 2.3 2.2 3.3 4.1 2.6 3.8 0 0 0 3.0 0 0
Cytoplasm 1.5 -1.2 -1.1 -3.3 -5.5 -4.9 -5.2 -4.9 -6.3 -0.1 0.3 0.9 -0.7 -1.2 -0.6
Endoplasmic Reticulum 0 0 0 2.0 4.5 3.4 3.5 3.8 4.6 0 0 0 0 2.1 1.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.8758 15.6343 13.8793 14.6639 13.6884 14.1711 14.7778 17.0148 14.8497 14.5489 14.9484 15.3649 14.8519 18.2295 15.8645 13.4876 16.3417 17.1302
Actin 0.0006 0.0007 0.0008 0 0.0001 0.0001 0.0011 0.0019 0.001 0.0011 0.0004 0.0008 0.0181 0.0054 0.0047 0.0022 0.0046 0.0016
Bud 0.0001 0.0001 0.0014 0.0001 0.0001 0.0001 0.0001 0.0035 0.0017 0.0032 0.0001 0.0005 0.005 0.0079 0.0015 0.0002 0.0005 0.0002
Bud Neck 0.0003 0.0002 0.0004 0.0003 0.0004 0.0007 0.0003 0.0002 0.0003 0.0008 0.0006 0.0008 0.0024 0.0002 0.0007 0.0006 0.0008 0.0037
Bud Periphery 0 0 0.0004 0 0 0 0.0001 0.0008 0.0008 0.0059 0 0.0002 0.0013 0.0015 0.0008 0.0001 0.0005 0.0001
Bud Site 0.0001 0.0002 0.0006 0.0001 0 0 0.0002 0.0014 0.0003 0.0002 0.0001 0.0001 0.0028 0.0016 0.008 0.0006 0.0006 0.0001
Cell Periphery 0 0 0.0001 0 0 0 0 0 0 0.0002 0 0 0.0003 0.0001 0.0003 0.0001 0.0001 0
Cytoplasm 0.9746 0.9893 0.9669 0.9708 0.9861 0.9727 0.9408 0.9847 0.9742 0.975 0.9792 0.9683 0.8208 0.9674 0.9318 0.8304 0.9235 0.9637
Cytoplasmic Foci 0.0017 0.0012 0.0018 0.001 0.0014 0.0028 0.0145 0.0011 0.0021 0.0011 0.0018 0.0057 0.0339 0.0035 0.0055 0.0164 0.0081 0.0041
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0026 0.001 0.0035 0.0011 0.0005 0.002 0.0042 0.0007 0.0019 0.001 0.0018 0.0018 0.0094 0.0004 0.0043 0.0085 0.0099 0.0052
Endosome 0.0017 0.0007 0.0026 0.0017 0.0005 0.0041 0.0058 0.0003 0.0025 0.0005 0.0013 0.0035 0.0224 0.0002 0.0084 0.0557 0.0276 0.0048
Golgi 0.0001 0.0001 0.0004 0 0 0.0001 0.0004 0 0.0006 0.0001 0 0.0007 0.007 0 0.0018 0.0181 0.0028 0.0007
Lipid Particles 0 0 0.0002 0 0 0 0.0025 0 0 0 0 0.0002 0.0095 0.0001 0.0022 0.0249 0.0017 0.0001
Mitochondria 0.0002 0.0001 0.002 0.0001 0.0001 0.0001 0.0004 0.0002 0.0003 0.0006 0.0001 0.0014 0.012 0.0005 0.0063 0.0072 0.0013 0.0011
None 0.0021 0.0006 0.0025 0.0006 0.0006 0.0009 0.0041 0.0006 0.0014 0.0005 0.0008 0.0013 0.005 0.0014 0.0026 0.0061 0.0011 0.001
Nuclear Periphery 0.0039 0.0009 0.0028 0.0026 0.0013 0.0026 0.0046 0.0007 0.0018 0.0012 0.0026 0.002 0.0133 0.0007 0.0052 0.0062 0.0039 0.0026
Nucleolus 0 0 0.0007 0 0 0 0.0008 0 0 0 0 0 0.002 0.0002 0.0008 0.0001 0.0001 0
Nucleus 0.0102 0.0042 0.0081 0.017 0.0083 0.0117 0.0107 0.0031 0.0094 0.0076 0.0098 0.0099 0.0166 0.0076 0.0087 0.0059 0.0043 0.0089
Peroxisomes 0 0 0.0001 0 0 0 0.0006 0.0001 0.0001 0 0 0.0004 0.0066 0.0001 0.0014 0.0088 0.0003 0.0001
Punctate Nuclear 0.0005 0.0002 0.0033 0.0002 0.0002 0.0005 0.0065 0.0003 0.0008 0.0003 0.0005 0.0013 0.0029 0.0004 0.0023 0.0008 0.0006 0.0008
Vacuole 0.0008 0.0005 0.0011 0.0037 0.0004 0.0012 0.0017 0.0003 0.0005 0.0006 0.0006 0.0007 0.0068 0.0007 0.0017 0.0053 0.0066 0.0008
Vacuole Periphery 0.0002 0.0001 0.0005 0.0005 0.0001 0.0003 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002 0.0018 0.0001 0.001 0.0017 0.0012 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.2564 60.2445 115.1697 109.2392 110.3319 90.1871 73.3023 80.4825 126.7611 94.4308
Translational Efficiency 1.971 2.8413 1.4058 2.4239 2.0071 1.4685 1.7501 2.1508 1.4954 1.8198

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
70 1053 1806 1114 1515 2515 538 1036 1585 3568 2344 2150

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3588.77 4087.07 3517.42 2208.28 3820.03 4184.82 4664.36 2366.39 3809.82 4155.97 3780.67 2284.47
Standard Deviation 611.65 883.33 733.38 470.19 770.90 793.14 786.21 524.33 766.04 822.00 888.20 503.25
Intensity Change Log 2 — 0.187577 -0.028972 -0.700566 — 0.131582 0.288095 -0.690896 — 0.158977 0.143175 -0.695572

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000175 0.000076 0.000058 0.000669 0.000058 0.000025 0.000188 0.000308 0.000063 0.000040 0.000088 0.000495
Bud Neck 0.000858 0.000550 0.000547 0.001462 0.000471 0.000111 0.003103 0.001275 0.000489 0.000240 0.001133 0.001372
Bud Site 0.001548 0.001771 0.000818 0.009096 0.000559 0.001725 0.001403 0.004346 0.000603 0.001738 0.000953 0.006807
Cell Periphery 0.001089 0.002448 0.000399 0.000686 0.000763 0.000378 0.002001 0.000397 0.000777 0.000989 0.000767 0.000547
Cytoplasm 0.811666* 0.894696* 0.824822* 0.867734* 0.855149* 0.960550* 0.679879* 0.868334* 0.853228* 0.941115* 0.791554* 0.868023*
Cytoplasmic Foci 0.014068 0.009987 0.003034 0.008451 0.003305 0.003724 0.018311 0.010191 0.003780 0.005573 0.006541 0.009289
Eisosomes 0.000001 0.000001 0.000001 0.000003 0.000001 0.000000 0.000003 0.000002 0.000001 0.000000 0.000001 0.000002
Endoplasmic Reticulum 0.000053 0.000087 0.000021 0.000216 0.000037 0.000062 0.000038 0.000069 0.000038 0.000070 0.000025 0.000145
Endosome 0.000130 0.000310 0.000052 0.001916 0.000066 0.000148 0.000188 0.001351 0.000069 0.000196 0.000084 0.001643
Golgi 0.002164 0.019527 0.000551 0.003814 0.002166 0.004917 0.001779 0.003405 0.002166 0.009229 0.000833 0.003617
Lipid Particles 0.001950 0.003979 0.000612 0.003534 0.000860 0.000651 0.002671 0.003171 0.000908 0.001633 0.001084 0.003359
Mitochondria 0.000053 0.000106 0.000099 0.000419 0.000096 0.000098 0.000129 0.000091 0.000095 0.000100 0.000105 0.000261
Mitotic Spindle 0.000972 0.000873 0.001015 0.002418 0.000203 0.000826 0.000497 0.007120 0.000237 0.000840 0.000896 0.004684
None 0.003947 0.006138 0.006933 0.003731 0.012058 0.003440 0.008022 0.003357 0.011699 0.004236 0.007183 0.003551
Nuclear Periphery 0.000129 0.000165 0.000113 0.000083 0.000098 0.000055 0.000492 0.000212 0.000099 0.000087 0.000200 0.000145
Nuclear Periphery Foci 0.004060 0.007062 0.000908 0.003146 0.002258 0.001570 0.002956 0.004745 0.002337 0.003191 0.001378 0.003916
Nucleolus 0.000055 0.000187 0.000079 0.000120 0.000054 0.000060 0.000115 0.000101 0.000054 0.000098 0.000087 0.000111
Nucleus 0.147666* 0.023709 0.150500* 0.057661 0.115999 0.010867 0.249344* 0.061333 0.117398 0.014657 0.173187* 0.059431
Peroxisomes 0.000007 0.000009 0.000009 0.000122 0.000003 0.000017 0.000010 0.000053 0.000003 0.000015 0.000009 0.000089
Vacuole 0.009256 0.021471 0.009179 0.031172 0.004714 0.007281 0.028567 0.028353 0.004914 0.011469 0.013629 0.029814
Vacuole Periphery 0.000151 0.006846 0.000249 0.003547 0.001084 0.003496 0.000302 0.001789 0.001043 0.004485 0.000261 0.002700

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.75 2.45 -1.47 -2.72 -2.58 5.80 -8.47 -2.64 -3.12 0.29 4.08 -7.04 -3.61 -3.99 -2.75
Bud Neck 1.34 0.38 -0.59 -6.97 -3.91 2.09 -9.56 -6.96 -13.54 7.05 1.58 -7.78 -7.11 -16.63 2.34
Bud Site 0.23 1.33 -5.39 -6.08 -6.95 -3.54 -7.17 -10.25 -7.99 -5.12 -4.08 -4.75 -10.78 -9.01 -9.35
Cell Periphery -1.91 2.09 1.86 8.65 -1.51 3.35 -9.06 2.64 -1.71 12.45 -1.13 -1.68 2.23 5.42 7.20
Cytoplasm -4.94 0.31 -1.75 9.13 -6.57 -27.15 26.05 7.09 26.98 -19.83 -22.30 20.07 6.34 29.28 -13.81
Cytoplasmic Foci 1.82 2.62 1.52 -1.33 -6.38 0.63 -12.20 -11.65 -13.24 7.34 -1.55 -9.58 -11.93 -12.81 -0.51
Eisosomes 1.76 1.41 -0.60 -8.03 -7.79 4.52 -11.97 -6.39 -7.65 6.21 2.82 -10.43 -10.31 -12.25 -2.38
Endoplasmic Reticulum -1.34 2.75 -1.38 -0.84 -2.13 -2.14 -3.49 -1.61 0.63 1.25 -3.24 0.40 -2.12 -0.94 -2.22
Endosome -2.14 1.36 -2.70 -2.30 -2.97 -3.06 -6.24 -3.02 -2.69 -1.84 -5.38 -3.87 -4.17 -3.57 -3.80
Golgi -11.35 3.76 -0.15 11.18 -3.50 -4.71 0.77 0.71 4.46 0.25 -10.85 3.62 0.37 10.96 -2.94
Lipid Particles -0.39 1.51 -0.68 -0.64 -5.15 1.85 -8.07 -6.91 -7.70 0.06 -2.70 -4.48 -8.13 -6.93 -5.88
Mitochondria -2.56 -6.14 -1.83 -1.21 -1.19 0.29 -5.53 2.74 2.34 6.45 -0.27 -3.71 -0.96 -0.89 -0.30
Mitotic Spindle -0.39 -0.44 -0.87 -0.56 -0.49 -2.05 -0.93 -2.76 -2.35 -2.52 -2.53 -1.98 -3.47 -2.53 -2.12
None -1.66 -4.82 2.33 2.59 9.02 8.11 5.76 9.60 1.97 7.23 7.06 5.76 9.52 3.10 12.78
Nuclear Periphery 0.43 -0.82 1.35 3.24 7.27 3.08 -13.38 -2.88 -4.06 9.87 1.54 -13.56 -2.80 -3.72 8.55
Nuclear Periphery Foci -1.49 3.73 1.35 3.65 -8.71 2.19 -5.15 -4.54 -6.21 -1.01 -1.73 0.73 -4.87 -3.17 -7.06
Nucleolus -1.11 -6.95 -5.06 0.63 -1.20 -0.21 -9.41 -5.60 -2.60 5.45 -1.12 -7.13 -5.90 -0.45 0.68
Nucleus 7.90 -1.24 5.29 -14.84 25.73 34.06* -19.77 12.16 -23.64 26.82 33.68 -17.06 14.82 -29.63 35.02
Peroxisomes -0.46 -0.24 -3.43 -3.22 -3.42 -6.83 -7.15 -7.34 -5.48 -4.37 -5.22 -8.55 -5.62 -4.64 -4.79
Vacuole -1.46 -1.28 -9.51 -11.12 -12.75 -1.72 -12.88 -19.01 -18.48 -1.37 -5.65 -15.98 -25.75 -22.99 -13.42
Vacuole Periphery -9.15 -1.27 -2.46 5.02 -2.33 -3.64 1.28 0.41 4.93 -1.10 -5.55 1.44 -0.48 5.93 -2.65
ORF
Human Ortholog
Description Protein of unknown function; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

YLR257W

YLR257W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ylr257w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available