Standard name
Human Ortholog
Description Rab family GTPase; required for endosome-to-Golgi, intra-Golgi retrograde, and retrograde Golgi-to-ER transport; temporarily at the Golgi, dissociating into the cytosol on arrival of the late Golgi GTPase Ypt32p; Golgi-localized form is GTP bound, while cytosolic form is GDP-bound; required for delivery of Atg9p to the phagophore assembly site during autophagy under heat stress, with Ypt6p for starvation induced autophagy and for the CVT pathway; homolog of mammalian Rab6
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.96 0.97 0.94 0.88 0.81 0.83 – 0.65 0.7 0.7 0.97 0.99 0.98 0.95 – – 0.82 0.83 0.76 0.75 0.74 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0.05 0 0.06 0.11 0.13 0.27 0.36 0.38 – 0.48 0.55 0.53 0 0 0 0.07 – – 0.07 0.07 0.12 0.08 0.12 0.09
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – 3 3 1 1 1 2
Bud 0 3 0 1 1 3 6 7 – 9 7 9 1 0 0 0 – – 1 0 1 1 1 6
Bud Neck 1 2 3 1 1 6 4 1 – 3 4 1 2 1 3 3 – – 1 1 0 3 4 1
Bud Site 0 0 0 0 0 0 0 0 – 0 3 2 0 0 0 0 – – – – – – – –
Cell Periphery 0 4 2 2 1 1 2 1 – 2 2 1 3 7 0 1 – – 1 4 2 0 2 1
Cytoplasm 173 276 183 148 183 240 244 318 – 186 192 251 184 190 238 87 – – 150 233 145 92 147 165
Endoplasmic Reticulum 2 2 1 0 0 0 0 0 – 0 1 0 2 0 0 5 – – 0 0 1 0 1 2
Endosome 0 0 0 0 1 0 0 0 – 0 0 0 0 0 1 3 – – 2 1 2 1 1 2
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 1 1 0 – – 1 0 0 0 0 1
Mitochondria 9 5 12 16 25 73 109 147 – 139 152 191 0 0 1 6 – – 13 20 23 10 24 19
Nucleus 0 0 0 0 1 2 4 4 – 1 2 0 1 0 1 1 – – 0 0 0 0 0 1
Nuclear Periphery 0 1 1 0 0 0 1 0 – 0 0 0 0 1 0 2 – – 0 0 0 0 0 0
Nucleolus 0 1 0 1 0 0 0 0 – 0 1 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 – 0 0 0 0 0 0 0 – – 0 0 1 0 0 0
SpindlePole 1 0 0 2 1 1 9 4 – 5 12 12 0 0 0 0 – – 1 1 1 0 2 2
Vac/Vac Membrane 0 1 0 0 0 0 1 1 – 1 2 2 0 1 0 0 – – 1 3 2 6 6 8
Unique Cell Count 181 283 190 152 195 272 302 385 287 276 358 189 192 242 92 184 280 190 123 198 221
Labelled Cell Count 186 295 202 171 216 326 380 483 346 378 469 193 201 245 108 184 280 190 123 198 221


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.5 3.2 3.4 3.4 3.2 2.9 2.8 – 2.5 2.5 2.3 4.6 4.6 4.6 4.9 4.7 4.8 4.6 5.1 5.1
Std Deviation (1e-4) 1.0 0.5 1.1 1.9 2.0 1.8 2.2 1.5 – 1.3 1.4 0.9 0.9 1.2 1.2 1.2 1.3 1.2 0.7 1.5 1.3
Intensity Change (Log2) – – – 0.09 0.09 -0.0 -0.13 -0.2 – -0.37 -0.34 -0.47 0.52 0.53 0.53 0.62 0.54 0.59 0.53 0.67 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.1733 -0.3467 -0.3587 -0.6395 -0.6535 -0.3375 0.7503 0.9986 1.3134 0.939 0.6048 1.1891 -1.8471 -1.3355 -1.7411 -1.3297 -1.8527 -1.5763
Actin 0.018 0.0002 0.0062 0.039 0.0232 0.006 0.0222 0.0002 0.0179 0.0344 0.0029 0.007 0.0011 0.0001 0.0001 0.0006 0.0001 0.0002
Bud 0.0003 0.0018 0.0005 0.0005 0.0001 0.0012 0.0004 0.0001 0.0002 0.0002 0 0.0009 0.0005 0 0.0002 0.0004 0 0
Bud Neck 0.0051 0.0003 0.0002 0.0001 0.0001 0.0068 0.0043 0 0.0012 0.0014 0.0001 0.0001 0.0003 0.0001 0.0001 0 0 0.0001
Bud Periphery 0.0005 0.0011 0.0014 0.0008 0.0001 0.0015 0.0008 0.0002 0.0003 0.0005 0.0001 0.0038 0.0005 0 0.0002 0.0006 0.0001 0
Bud Site 0.0081 0.0113 0.0019 0.0003 0.0001 0.0004 0.0026 0.0014 0.0007 0.0016 0.0001 0.0001 0.0011 0.0002 0.0002 0.0002 0 0
Cell Periphery 0.0002 0.0003 0.0001 0.0001 0 0.0001 0.0006 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0001 0 0 0 0
Cytoplasm 0.0135 0.0243 0.0123 0.0124 0.0294 0.0166 0.0273 0.0083 0.0237 0.0112 0.0115 0.014 0.0168 0.0076 0.0176 0.0086 0.0134 0.0186
Cytoplasmic Foci 0.0045 0.0027 0.0043 0.0016 0.0077 0.0051 0.0117 0.0018 0.0041 0.0066 0.0154 0.0026 0.0045 0.0013 0.0059 0.003 0.0028 0.0021
Eisosomes 0.0005 0.0001 0.0002 0.0001 0.0002 0.0002 0.0005 0.0002 0.0003 0.0008 0 0.0002 0.0005 0.0005 0.0005 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0022 0.0003 0.0009 0.0004 0.0005 0.0005 0.0026 0.0001 0.0005 0.0005 0.0001 0.003 0.0003 0.0001 0.0003 0.0003 0.0001 0.0001
Endosome 0.0046 0.0003 0.0029 0.0128 0.0055 0.0024 0.0115 0 0.002 0.0019 0.0012 0.004 0.0011 0.0001 0.0006 0.0001 0.0003 0.0001
Golgi 0.0019 0 0.0013 0.0055 0.0019 0.0019 0.0033 0 0.0013 0.0024 0.0012 0.0004 0.0003 0 0.0001 0 0 0
Lipid Particles 0.0016 0.0001 0.0029 0.0005 0.0025 0.0008 0.0037 0.0001 0.0008 0.0076 0.0063 0.0003 0.0005 0.0003 0.0002 0.0001 0.0003 0.0001
Mitochondria 0.0012 0.0002 0.001 0.0158 0.0006 0.0009 0.001 0.0001 0.0005 0.0009 0.0001 0.0004 0.0003 0.0004 0.0003 0.0005 0.0001 0.0001
None 0.8974 0.9511 0.9496 0.9055 0.9102 0.9492 0.8809 0.9868 0.9331 0.9184 0.9202 0.9606 0.9657 0.9883 0.9638 0.9779 0.9819 0.9773
Nuclear Periphery 0.0166 0.0002 0.004 0.0003 0.0026 0.0005 0.0081 0 0.002 0.0002 0.0004 0.0014 0.0006 0 0.0003 0.0013 0.0001 0.0001
Nucleolus 0.0005 0.0004 0.0003 0.0001 0.0002 0.0004 0.0003 0.0001 0.0005 0.0003 0.0002 0 0.0005 0.0002 0.0005 0.0006 0.0001 0.0001
Nucleus 0.014 0.0009 0.0026 0.0002 0.0005 0.0013 0.005 0.0001 0.0059 0.0002 0.0001 0.0002 0.0009 0.0001 0.0023 0.0017 0.0001 0.0002
Peroxisomes 0.004 0.0001 0.0035 0.0006 0.0048 0.0013 0.0075 0.0001 0.0023 0.0094 0.0124 0.0004 0.0013 0.0003 0.0002 0.0002 0.0001 0.0001
Punctate Nuclear 0.0043 0.0038 0.0036 0.0002 0.0095 0.0026 0.0021 0.0001 0.0016 0.0007 0.0277 0.0005 0.0026 0.0002 0.0061 0.0034 0.0003 0.0007
Vacuole 0.0006 0.0003 0.0003 0.0015 0.0002 0.0002 0.003 0.0001 0.0006 0.0004 0 0.0001 0.0002 0.0001 0.0003 0.0002 0.0001 0
Vacuole Periphery 0.0004 0 0.0002 0.0017 0.0002 0.0001 0.0005 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.2043 28.9215 32.7563 46.8119 17.6619 42.546 40.4009 44.148 39.583 43.8158
Translational Efficiency 1.7957 2.588 1.802 1.2148 3.2874 1.5417 2.1127 1.6076 1.8915 1.7804

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1195 1201 1207 1444 1688 2167 1568 1953 2883 3368 2775 3397

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 525.60 653.23 780.25 786.95 637.68 660.77 781.00 816.69 591.22 658.08 780.67 804.05
Standard Deviation 59.07 80.74 83.32 108.43 117.30 76.17 84.04 101.99 112.03 77.91 83.73 105.80
Intensity Change Log 2 — 0.313626 0.569971 0.582307 — 0.051316 0.292490 0.356956 — 0.175767 0.424503 0.463152

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000207 0.000395 0.000078 0.002060 0.000058 0.000460 0.000090 0.000555 0.000120 0.000437 0.000085 0.001195
Bud Neck 0.036993 0.045604 0.001591 0.012120 0.025532 0.064669 0.003633 0.008299 0.030282 0.057871 0.002745 0.009924
Bud Site 0.008105 0.013537 0.003416 0.034037 0.005510 0.020825 0.007893 0.030399 0.006586 0.018226 0.005946 0.031945
Cell Periphery 0.000935 0.000166 0.000087 0.000200 0.001484 0.000313 0.000102 0.000127 0.001256 0.000261 0.000095 0.000158
Cytoplasm 0.332696* 0.314726* 0.447077* 0.467156* 0.410951* 0.291690* 0.412816* 0.516056* 0.378514* 0.299905* 0.427718* 0.495269*
Cytoplasmic Foci 0.325298* 0.329816* 0.015283 0.022590 0.121822 0.330614* 0.012860 0.020491 0.206162* 0.330330* 0.013914 0.021383
Eisosomes 0.000977 0.000217 0.000028 0.000051 0.002537 0.000300 0.000028 0.000039 0.001890 0.000271 0.000028 0.000044
Endoplasmic Reticulum 0.002027 0.000726 0.001251 0.002358 0.004823 0.000487 0.001006 0.000810 0.003664 0.000572 0.001113 0.001468
Endosome 0.008901 0.038751 0.001770 0.006720 0.009751 0.032314 0.001293 0.004944 0.009399 0.034609 0.001501 0.005699
Golgi 0.011824 0.011510 0.000056 0.004512 0.003656 0.015268 0.000052 0.002479 0.007042 0.013928 0.000054 0.003343
Lipid Particles 0.039179 0.015463 0.000995 0.001437 0.023571 0.014970 0.000813 0.000944 0.030041 0.015146 0.000892 0.001153
Mitochondria 0.064455 0.026448 0.000281 0.003218 0.005966 0.050546 0.000785 0.003077 0.030210 0.041953 0.000566 0.003137
Mitotic Spindle 0.000734 0.002378 0.001161 0.023436 0.000712 0.005479 0.002565 0.027656 0.000721 0.004373 0.001954 0.025862
None 0.008144 0.004157 0.005488 0.006321 0.035214 0.001732 0.004852 0.005117 0.023993 0.002597 0.005128 0.005629
Nuclear Periphery 0.000449 0.000516 0.001013 0.001972 0.001053 0.000253 0.001042 0.001218 0.000803 0.000347 0.001030 0.001538
Nuclear Periphery Foci 0.000454 0.000344 0.000694 0.001344 0.001415 0.000206 0.000301 0.000311 0.001017 0.000255 0.000472 0.000750
Nucleolus 0.001351 0.003138 0.000448 0.000718 0.002308 0.001466 0.000424 0.000524 0.001911 0.002062 0.000434 0.000606
Nucleus 0.117935 0.094888 0.487724* 0.315416* 0.276705* 0.078546 0.520896* 0.308340* 0.210895* 0.084374 0.506468* 0.311348*
Peroxisomes 0.024037 0.036200 0.000201 0.000665 0.005239 0.033425 0.000241 0.000803 0.013031 0.034415 0.000224 0.000744
Vacuole 0.013179 0.057123 0.031209 0.092002 0.060617 0.053474 0.028044 0.066768 0.040954 0.054775 0.029421 0.077495
Vacuole Periphery 0.002123 0.003897 0.000148 0.001668 0.001074 0.002961 0.000261 0.001045 0.001509 0.003295 0.000212 0.001309

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.42 3.58 -1.42 -1.09 -1.61 -5.96 -3.38 -2.50 0.58 -2.03 -6.85 0.57 -2.10 -0.82 -2.13
Bud Neck -2.19 14.50 10.63 11.83 -6.18 -12.65 12.32 9.93 21.81 -4.80 -11.34 18.93 14.49 24.52 -7.81
Bud Site -3.06 2.90 -4.07 -2.23 -6.26 -8.53 -0.95 -6.85 0.15 -5.70 -8.77 1.09 -7.81 -0.95 -8.41
Cell Periphery 3.93 4.30 3.99 1.05 -5.83 6.63 10.86 10.86 1.67 0.02 7.35 10.51 10.28 1.68 -3.91
Cytoplasm 1.79 -8.62 -3.69 -5.24 5.02 14.76 3.14 -3.69 -17.68 -6.38 12.20 -3.25 -5.03 -16.77 -1.54
Cytoplasmic Foci -0.51 48.10 47.78 46.76 -0.84 -33.05* 29.19 27.92 60.13 -3.18 -22.07 49.00 48.13 76.14 -2.94
Eisosomes 10.46 13.06 12.67 17.03 -9.44 7.13 7.98 7.94 16.55 -4.28 8.67 9.94 9.85 20.86 -9.52
Endoplasmic Reticulum 3.35 0.83 -3.08 -13.95 -8.43 9.29 7.08 7.42 -10.53 0.87 9.72 6.50 4.60 -17.54 -5.68
Endosome -13.25 11.10 6.46 15.60 -3.59 -14.27 12.41 8.02 19.33 -3.83 -19.54 16.40 10.25 24.83 -5.22
Golgi 0.39 12.69 8.52 7.17 -2.35 -10.32 8.75 3.86 12.26 -2.69 -7.48 15.20 9.05 14.04 -3.53
Lipid Particles 9.49 16.70 16.53 13.20 -3.13 5.21 17.18 17.14 14.33 -0.69 10.43 23.63 23.47 19.02 -2.77
Mitochondria 8.09 15.19 14.74 11.00 -3.21 -15.16 6.58 4.44 16.88 -2.40 -4.17 15.71 14.79 19.78 -3.78
Mitotic Spindle -2.91 -0.46 -6.76 -5.95 -6.54 -4.61 -2.19 -8.56 -6.12 -7.42 -5.31 -2.13 -10.90 -8.19 -9.84
None 2.87 2.31 2.14 -1.45 -0.40 9.74 8.96 8.93 -6.35 -0.08 10.17 9.18 9.11 -5.74 -0.34
Nuclear Periphery -0.75 -11.14 -20.21 -18.04 -14.90 11.14 -4.31 -9.79 -21.35 -7.36 8.14 -9.37 -20.76 -27.98 -15.81
Nuclear Periphery Foci 0.33 -1.75 -3.32 -6.68 -2.90 5.53 4.68 4.82 -3.03 0.74 4.64 2.47 1.29 -7.30 -2.42
Nucleolus -3.68 7.00 4.05 4.83 -2.36 3.64 8.72 8.30 6.34 -1.11 -0.67 10.64 9.19 7.03 -2.51
Nucleus 3.74 -40.61 -29.17 -32.36 12.12 31.62 -28.25 -9.64 -40.84 18.58 27.18 -45.41 -24.06 -52.51 21.94
Peroxisomes -4.37 15.02 14.89 15.75 -2.44 -16.31 7.51 7.05 20.65 -2.92 -14.03 16.34 16.00 25.98 -3.78
Vacuole -15.86 -11.12 -26.95 -18.28 -20.10 2.02 4.26 -12.01 -14.17 -15.20 -6.50 -1.83 -26.72 -22.96 -24.81
Vacuole Periphery -3.13 9.54 5.16 5.82 -3.60 -8.78 7.49 2.72 9.80 -2.40 -7.31 12.14 5.63 10.68 -4.14
Standard name
Human Ortholog
Description Rab family GTPase; required for endosome-to-Golgi, intra-Golgi retrograde, and retrograde Golgi-to-ER transport; temporarily at the Golgi, dissociating into the cytosol on arrival of the late Golgi GTPase Ypt32p; Golgi-localized form is GTP bound, while cytosolic form is GDP-bound; required for delivery of Atg9p to the phagophore assembly site during autophagy under heat stress, with Ypt6p for starvation induced autophagy and for the CVT pathway; homolog of mammalian Rab6
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Ypt6

Ypt6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypt6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available