Standard name | |
---|---|
Human Ortholog | |
Description | R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 29 | 28 | 14 | 16 | 5 | 17 | 10 | 20 | – | 16 | 9 | 14 | 26 | 24 | 17 | 28 | 16 | 17 | 40 | 36 | 18 | 9 | 33 | 39 |
Bud | 18 | 6 | 9 | 6 | 20 | 18 | 32 | 48 | – | 25 | 33 | 49 | 3 | 4 | 6 | 7 | 6 | 4 | 4 | 7 | 2 | 1 | 6 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 3 | 1 | 0 | 3 | 2 |
Bud Site | 2 | 0 | 0 | 0 | 1 | 8 | 4 | 5 | – | 8 | 3 | 8 | 0 | 0 | 0 | 0 | 2 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 0 | 2 | 2 | 0 | 1 | 3 | – | 4 | 1 | 8 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 54 | 108 | 61 | 92 | 108 | 128 | 121 | 125 | – | 99 | 68 | 105 | 49 | 74 | 81 | 36 | 25 | 23 | 7 | 41 | 8 | 2 | 6 | 12 |
Endoplasmic Reticulum | 23 | 51 | 6 | 30 | 20 | 34 | 50 | 33 | – | 39 | 13 | 23 | 29 | 19 | 12 | 3 | 3 | 0 | 20 | 30 | 21 | 5 | 20 | 32 |
Endosome | 94 | 15 | 41 | 10 | 48 | 53 | 39 | 69 | – | 16 | 14 | 31 | 28 | 52 | 30 | 9 | 8 | 15 | 112 | 69 | 43 | 25 | 51 | 53 |
Golgi | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 2 | 1 | 5 | 4 | 1 | 72 | 22 | 19 | 18 | 40 | 40 |
Mitochondria | 39 | 16 | 35 | 22 | 41 | 131 | 185 | 306 | – | 232 | 181 | 259 | 4 | 3 | 0 | 9 | 4 | 3 | 44 | 46 | 40 | 29 | 47 | 46 |
Nucleus | 0 | 1 | 0 | 1 | 1 | 2 | 2 | 4 | – | 1 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 5 | 3 | 14 | – | 1 | 1 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 6 | 0 | 8 | 1 | 8 | 6 | 7 | 11 | – | 2 | 1 | 8 | 0 | 4 | 0 | 0 | 1 | 0 | 14 | 4 | 7 | 3 | 9 | 5 |
SpindlePole | 25 | 6 | 6 | 5 | 3 | 16 | 12 | 25 | – | 26 | 13 | 14 | 11 | 17 | 20 | 9 | 11 | 7 | 13 | 5 | 7 | 3 | 5 | 4 |
Vac/Vac Membrane | 14 | 3 | 3 | 0 | 7 | 5 | 10 | 13 | – | 16 | 5 | 5 | 2 | 7 | 2 | 2 | 3 | 4 | 9 | 19 | 6 | 6 | 18 | 13 |
Unique Cell Count | 246 | 204 | 149 | 162 | 221 | 329 | 384 | 543 | 393 | 278 | 416 | 125 | 180 | 155 | 86 | 60 | 65 | 346 | 290 | 179 | 109 | 251 | 261 | |
Labelled Cell Count | 313 | 237 | 183 | 185 | 264 | 423 | 476 | 677 | 487 | 342 | 533 | 155 | 209 | 175 | 109 | 86 | 75 | 346 | 290 | 179 | 109 | 251 | 261 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 6.5 | 5.5 | 5.6 | 5.7 | 5.4 | 5.1 | 5.2 | – | 4.7 | 5.0 | 4.9 | 7.1 | 7.1 | 6.7 | 7.3 | 7.1 | 7.7 | 8.1 | 8.2 | 8.6 |
Std Deviation (1e-4) | 0.9 | 0.7 | 0.9 | 1.2 | 1.4 | 1.3 | 1.3 | 1.1 | – | 0.9 | 0.9 | 0.9 | 1.1 | 1.1 | 0.9 | 0.9 | 1.1 | 1.1 | 1.4 | 1.2 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.05 | -0.02 | -0.11 | -0.07 | – | -0.22 | -0.14 | -0.16 | 0.38 | 0.37 | 0.29 | 0.42 | 0.39 | 0.5 | 0.56 | 0.59 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.1 | -3.0 | -1.7 | -3.4 | -2.9 | – | 0 | -2.7 | 0 | 2.7 | 1.1 | 0.5 | 4.5 | 3.2 | 3.2 |
Bud | -1.0 | 1.1 | -0.2 | 0.9 | 1.1 | – | 0 | 1.9 | 0 | -1.5 | -1.8 | -0.9 | 0.6 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.8 | 1.5 | -0.4 | -2.1 | -4.4 | – | -3.6 | -3.5 | -3.6 | -0.3 | 0 | 2.0 | 0.1 | 0.1 | -0.8 |
Endoplasmic Reticulum | 4.0 | 1.9 | 2.3 | 3.0 | 1.0 | – | 0 | 0.3 | 0 | 4.7 | 2.2 | 1.4 | 0 | 0 | 0 |
Endosome | -5.1 | -1.3 | -2.9 | -5.0 | -4.4 | – | 0 | -6.6 | 0 | -1.0 | 0.3 | -1.7 | -3.1 | -2.2 | -0.7 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.3 | -1.2 | 3.5 | 5.2 | 7.1 | – | 0 | 8.2 | 0 | -4.8 | -6.2 | -6.4 | -2.5 | -2.8 | -3.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | -0.8 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | -1.5 | 0 | 0 | 0 | 0 |
SpindlePole | -0.4 | 0 | 0.4 | -0.5 | 0.3 | – | 0 | 0.3 | 0 | 1.6 | 1.9 | 2.8 | 1.9 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5588 | 3.831 | 3.4785 | 3.5056 | 3.4352 | 3.6796 | 4.1831 | 4.5768 | 5.2154 | 4.3782 | 4.2646 | 4.6812 | 2.5278 | 3.0388 | 2.9268 | 3.208 | 2.7465 | 3.0962 |
Actin | 0.0331 | 0.0113 | 0.0267 | 0.0114 | 0.0204 | 0.032 | 0.0427 | 0.0285 | 0.0357 | 0.01 | 0.0072 | 0.0543 | 0.0276 | 0.0143 | 0.0134 | 0.0031 | 0.0259 | 0.0157 |
Bud | 0.0006 | 0.0008 | 0.0014 | 0.0005 | 0.0006 | 0.0004 | 0.0005 | 0.0013 | 0.0011 | 0.0002 | 0.0002 | 0.0007 | 0.0004 | 0.0007 | 0.0013 | 0.0004 | 0.0021 | 0.0003 |
Bud Neck | 0.0052 | 0.001 | 0.0011 | 0.0012 | 0.0006 | 0.0034 | 0.0037 | 0.0009 | 0.0015 | 0.0004 | 0.0008 | 0.0034 | 0.0012 | 0.0007 | 0.001 | 0.0009 | 0.0017 | 0.0082 |
Bud Periphery | 0.0008 | 0.0006 | 0.0011 | 0.0003 | 0.0006 | 0.0003 | 0.0007 | 0.0009 | 0.0013 | 0.0001 | 0.0001 | 0.0007 | 0.0004 | 0.0003 | 0.0011 | 0.0005 | 0.0028 | 0.0002 |
Bud Site | 0.004 | 0.0085 | 0.0033 | 0.0014 | 0.006 | 0.0007 | 0.0025 | 0.007 | 0.0053 | 0.0004 | 0.0006 | 0.001 | 0.0032 | 0.0073 | 0.0042 | 0.0012 | 0.011 | 0.0005 |
Cell Periphery | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0006 | 0.0001 |
Cytoplasm | 0.065 | 0.1856 | 0.0937 | 0.0811 | 0.0507 | 0.0624 | 0.0792 | 0.1108 | 0.1057 | 0.0531 | 0.0326 | 0.0889 | 0.0527 | 0.1408 | 0.1031 | 0.0777 | 0.0859 | 0.0841 |
Cytoplasmic Foci | 0.1851 | 0.2253 | 0.2178 | 0.2742 | 0.1719 | 0.2417 | 0.2394 | 0.2411 | 0.2065 | 0.2538 | 0.2176 | 0.2068 | 0.2385 | 0.2626 | 0.2328 | 0.2226 | 0.2566 | 0.2793 |
Eisosomes | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0307 | 0.0159 | 0.0129 | 0.0057 | 0.0159 | 0.0169 | 0.0137 | 0.0268 | 0.0263 | 0.0053 | 0.0581 | 0.0367 | 0.0287 | 0.0228 | 0.0243 | 0.0067 | 0.0029 | 0.0112 |
Endosome | 0.3645 | 0.3215 | 0.3619 | 0.3132 | 0.4437 | 0.336 | 0.2588 | 0.2586 | 0.2699 | 0.2782 | 0.3832 | 0.2567 | 0.36 | 0.3356 | 0.3568 | 0.3652 | 0.3496 | 0.3169 |
Golgi | 0.1562 | 0.1481 | 0.2152 | 0.2427 | 0.1889 | 0.2058 | 0.2205 | 0.21 | 0.2343 | 0.3316 | 0.204 | 0.2092 | 0.1761 | 0.1389 | 0.1722 | 0.1333 | 0.176 | 0.1868 |
Lipid Particles | 0.0393 | 0.0243 | 0.0178 | 0.024 | 0.0382 | 0.036 | 0.0366 | 0.0127 | 0.0106 | 0.0109 | 0.0186 | 0.0186 | 0.0362 | 0.0139 | 0.0175 | 0.0147 | 0.0146 | 0.0181 |
Mitochondria | 0.0217 | 0.011 | 0.0128 | 0.0072 | 0.0278 | 0.01 | 0.0048 | 0.0072 | 0.0133 | 0.0061 | 0.0063 | 0.0277 | 0.0211 | 0.0082 | 0.0158 | 0.0511 | 0.0055 | 0.0144 |
None | 0.0237 | 0.0136 | 0.0028 | 0.0014 | 0.0037 | 0.008 | 0.0407 | 0.0377 | 0.0587 | 0.0005 | 0.0007 | 0.0347 | 0.0105 | 0.0208 | 0.0109 | 0.0425 | 0.0256 | 0.0163 |
Nuclear Periphery | 0.0124 | 0.0022 | 0.0018 | 0.0006 | 0.0032 | 0.003 | 0.0026 | 0.0022 | 0.0014 | 0.0005 | 0.0013 | 0.0021 | 0.0041 | 0.0021 | 0.0023 | 0.0252 | 0.0008 | 0.001 |
Nucleolus | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0001 |
Nucleus | 0.0107 | 0.0009 | 0.0005 | 0.0002 | 0.001 | 0.0005 | 0.0004 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0.0018 | 0.0007 | 0.0032 | 0.0005 | 0.0005 |
Peroxisomes | 0.0228 | 0.0208 | 0.0216 | 0.0276 | 0.0144 | 0.0359 | 0.0473 | 0.0471 | 0.0196 | 0.0461 | 0.0637 | 0.0522 | 0.0272 | 0.0126 | 0.0308 | 0.0388 | 0.0309 | 0.0398 |
Punctate Nuclear | 0.0116 | 0.0013 | 0.0009 | 0.0042 | 0.004 | 0.0019 | 0.0014 | 0.001 | 0.002 | 0.0005 | 0.0006 | 0.0011 | 0.0041 | 0.0095 | 0.0032 | 0.0028 | 0.0019 | 0.0011 |
Vacuole | 0.0066 | 0.005 | 0.0044 | 0.0017 | 0.0052 | 0.0029 | 0.0022 | 0.0035 | 0.0041 | 0.0011 | 0.0025 | 0.0025 | 0.0044 | 0.0047 | 0.0054 | 0.0063 | 0.0032 | 0.0033 |
Vacuole Periphery | 0.0036 | 0.0016 | 0.0021 | 0.0012 | 0.0027 | 0.0016 | 0.0012 | 0.0017 | 0.0014 | 0.001 | 0.0015 | 0.0018 | 0.0019 | 0.0018 | 0.0024 | 0.0033 | 0.0014 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 56.4533 | 44.3487 | 29.5043 | 50.9488 | 52.1231 | 41.0342 | 39.8156 | 46.9623 | 37.8604 | 48.1123 |
Translational Efficiency | 1.4464 | 2.2012 | 2.2691 | 1.302 | 1.3085 | 2.1654 | 1.9578 | 1.7534 | 2.0082 | 1.466 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
325 | 1115 | 2105 | 1053 | 1828 | 2082 | 2642 | 1488 | 2153 | 3197 | 4747 | 2541 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 664.67 | 889.42 | 1067.27 | 1046.98 | 798.59 | 876.08 | 1114.13 | 1087.42 | 778.37 | 880.73 | 1093.35 | 1070.66 |
Standard Deviation | 73.71 | 118.24 | 130.93 | 155.71 | 158.42 | 138.96 | 133.21 | 161.82 | 156.29 | 132.26 | 134.24 | 160.55 |
Intensity Change Log 2 | — | 0.420227 | 0.683215 | 0.655524 | — | 0.133608 | 0.480391 | 0.445382 | — | 0.270891 | 0.576068 | 0.544644 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001510 | 0.015365 | 0.008931 | 0.054051 | 0.004590 | 0.004333 | 0.058037 | 0.065378 | 0.004125 | 0.008181 | 0.036261 | 0.060684 |
Bud Neck | 0.025027 | 0.003302 | 0.001957 | 0.005854 | 0.003687 | 0.002892 | 0.001747 | 0.003832 | 0.006909 | 0.003035 | 0.001840 | 0.004670 |
Bud Site | 0.038951 | 0.012614 | 0.013146 | 0.060899 | 0.007434 | 0.010191 | 0.016103 | 0.036275 | 0.012191 | 0.011036 | 0.014792 | 0.046479 |
Cell Periphery | 0.000922 | 0.000178 | 0.000092 | 0.000400 | 0.000298 | 0.000082 | 0.000207 | 0.000635 | 0.000392 | 0.000115 | 0.000156 | 0.000537 |
Cytoplasm | 0.116478* | 0.019795 | 0.030069 | 0.053024 | 0.026454 | 0.018965 | 0.021717 | 0.043389 | 0.040043 | 0.019255 | 0.025421 | 0.047382 |
Cytoplasmic Foci | 0.363940* | 0.322067* | 0.397292* | 0.308950* | 0.288159* | 0.298510* | 0.287524* | 0.310554* | 0.299598* | 0.306726* | 0.336199* | 0.309889* |
Eisosomes | 0.000912 | 0.000439 | 0.000075 | 0.000286 | 0.000515 | 0.000195 | 0.000428 | 0.000512 | 0.000575 | 0.000280 | 0.000271 | 0.000418 |
Endoplasmic Reticulum | 0.002164 | 0.004570 | 0.020072 | 0.018136 | 0.004188 | 0.001291 | 0.021519 | 0.014151 | 0.003883 | 0.002434 | 0.020877 | 0.015802 |
Endosome | 0.145330 | 0.167064 | 0.162171 | 0.176605 | 0.128742 | 0.246575* | 0.167250 | 0.189423 | 0.131246 | 0.218845* | 0.164998 | 0.184112 |
Golgi | 0.134042* | 0.360785* | 0.154044* | 0.110051* | 0.398045* | 0.303786* | 0.256558* | 0.195482* | 0.358193* | 0.323666* | 0.211099* | 0.160079* |
Lipid Particles | 0.032468 | 0.026354 | 0.072726 | 0.066895 | 0.067518 | 0.023812 | 0.114151* | 0.059344 | 0.062227 | 0.024699 | 0.095782 | 0.062473 |
Mitochondria | 0.036027 | 0.010245 | 0.002104 | 0.001873 | 0.017318 | 0.021677 | 0.003072 | 0.001559 | 0.020142 | 0.017690 | 0.002643 | 0.001689 |
Mitotic Spindle | 0.000635 | 0.005063 | 0.064873 | 0.034180 | 0.003175 | 0.008012 | 0.013068 | 0.008594 | 0.002792 | 0.006984 | 0.036040 | 0.019197 |
None | 0.000408 | 0.000240 | 0.000083 | 0.000063 | 0.007239 | 0.000648 | 0.000030 | 0.000033 | 0.006208 | 0.000506 | 0.000053 | 0.000046 |
Nuclear Periphery | 0.000144 | 0.000062 | 0.000770 | 0.000146 | 0.000318 | 0.000069 | 0.000104 | 0.000046 | 0.000292 | 0.000066 | 0.000399 | 0.000088 |
Nuclear Periphery Foci | 0.000241 | 0.000688 | 0.017890 | 0.004886 | 0.004320 | 0.000342 | 0.003122 | 0.001483 | 0.003704 | 0.000462 | 0.009671 | 0.002893 |
Nucleolus | 0.000339 | 0.000361 | 0.000100 | 0.000078 | 0.002156 | 0.000193 | 0.000019 | 0.000037 | 0.001881 | 0.000252 | 0.000055 | 0.000054 |
Nucleus | 0.004988 | 0.000806 | 0.001060 | 0.001164 | 0.004203 | 0.001781 | 0.000357 | 0.000915 | 0.004322 | 0.001441 | 0.000668 | 0.001018 |
Peroxisomes | 0.044599 | 0.031430 | 0.006566 | 0.033428 | 0.022176 | 0.024852 | 0.014368 | 0.031987 | 0.025561 | 0.027146 | 0.010908 | 0.032584 |
Vacuole | 0.040170 | 0.013681 | 0.042919 | 0.067195 | 0.006395 | 0.024375 | 0.018877 | 0.034603 | 0.011494 | 0.020645 | 0.029538 | 0.048109 |
Vacuole Periphery | 0.010706 | 0.004891 | 0.003061 | 0.001836 | 0.003069 | 0.007417 | 0.001742 | 0.001768 | 0.004222 | 0.006536 | 0.002327 | 0.001796 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.38 | -7.74 | -11.91 | -7.86 | -9.92 | 0.33 | -19.24 | -15.26 | -15.30 | -1.30 | -4.89 | -19.29 | -18.97 | -17.17 | -6.87 |
Bud Neck | 6.64 | 7.12 | 5.74 | -4.25 | -8.98 | 1.68 | 4.38 | -0.50 | -2.69 | -6.63 | 5.79 | 7.95 | 2.93 | -5.23 | -11.00 |
Bud Site | 3.87 | 3.82 | -3.03 | -12.23 | -12.70 | -1.99 | -7.24 | -12.60 | -11.26 | -8.72 | 0.77 | -2.12 | -14.77 | -16.65 | -15.26 |
Cell Periphery | 2.93 | 3.28 | 2.09 | -6.22 | -10.65 | 5.16 | 2.02 | -2.74 | -5.04 | -3.79 | 5.29 | 4.44 | -1.53 | -6.39 | -5.68 |
Cytoplasm | 11.25 | 9.78 | 5.75 | -9.93 | -7.02 | 3.37 | 1.58 | -6.35 | -9.01 | -7.90 | 8.77 | 5.42 | -4.68 | -13.39 | -10.42 |
Cytoplasmic Foci | 3.44 | -2.40 | 5.27 | 3.19 | 12.91 | -1.63 | 0.63 | -2.59 | -1.20 | -3.40 | -1.20 | -6.80 | -0.26 | 0.95 | 6.75 |
Eisosomes | 3.59 | 6.80 | 5.09 | 3.16 | -10.62 | 4.33 | 1.18 | 0.16 | -7.29 | -1.75 | 4.40 | 4.70 | 2.43 | -4.13 | -5.27 |
Endoplasmic Reticulum | -2.16 | -10.73 | -6.75 | -5.15 | 1.10 | 4.10 | -10.46 | -5.56 | -7.69 | 3.51 | 2.06 | -13.62 | -7.95 | -9.32 | 3.36 |
Endosome | -2.15 | -1.63 | -2.04 | 0.11 | -0.98 | -20.92 | -9.04 | -10.62 | 9.97 | -3.64 | -18.99 | -9.04 | -10.40 | 8.90 | -3.49 |
Golgi | -18.88 | -1.63 | 3.22 | 30.73* | 8.94 | 10.99 | 19.09 | 25.77 | 15.69 | 10.24 | 4.42 | 22.62 | 29.64 | 30.56 | 12.40 |
Lipid Particles | 1.36 | -8.41 | -6.07 | -9.03 | 1.65 | 11.14 | -9.50 | 2.02 | -10.46 | 12.70 | 11.20 | -8.66 | 0.34 | -13.87 | 10.82 |
Mitochondria | 5.67 | 7.64 | 7.59 | 6.00 | 0.31 | -2.29 | 10.27 | 11.33 | 13.37 | 2.45 | 1.44 | 13.02 | 13.32 | 14.42 | 1.81 |
Mitotic Spindle | -4.31 | -18.93 | -9.03 | -7.64 | 5.72 | -4.03 | -8.33 | -4.48 | -0.76 | 2.43 | -4.74 | -19.55 | -9.54 | -6.72 | 6.59 |
None | 0.85 | 2.22 | 2.33 | 1.21 | 0.63 | 5.07 | 5.76 | 5.75 | 1.53 | -0.87 | 5.24 | 5.78 | 5.78 | 1.72 | 0.29 |
Nuclear Periphery | 1.73 | -7.21 | -0.92 | -5.26 | 7.45 | 3.31 | 2.56 | 3.41 | 0.73 | 2.93 | 3.50 | -1.75 | 2.76 | -4.17 | 8.03 |
Nuclear Periphery Foci | -1.45 | -11.51 | -5.97 | -3.80 | 7.22 | 5.49 | 1.71 | 3.73 | -5.88 | 3.80 | 4.75 | -5.76 | 1.17 | -5.71 | 8.16 |
Nucleolus | -0.38 | 3.49 | 3.47 | 1.52 | 0.17 | 4.87 | 5.33 | 5.27 | 1.65 | -3.72 | 4.68 | 5.34 | 5.31 | 2.18 | -0.82 |
Nucleus | 4.02 | 3.78 | 2.91 | -2.87 | -2.55 | 2.43 | 4.99 | 3.40 | 0.53 | -2.60 | 3.65 | 5.40 | 3.88 | -0.37 | -3.45 |
Peroxisomes | 2.37 | 7.39 | 2.09 | -0.44 | -10.92 | -1.39 | 4.91 | -4.19 | -2.95 | -7.97 | -0.88 | 10.02 | -3.39 | -2.73 | -13.15 |
Vacuole | 4.45 | -1.03 | -6.63 | -14.85 | -8.19 | -10.38 | -12.03 | -12.94 | -5.82 | -7.67 | -5.95 | -13.62 | -18.01 | -14.41 | -10.71 |
Vacuole Periphery | 3.56 | 4.94 | 5.82 | 5.41 | 4.52 | -8.29 | 4.82 | 4.40 | 11.36 | 0.08 | -4.85 | 5.56 | 7.32 | 12.54 | 3.32 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|