Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0.05 0 0 – 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.31 0.31 0.21 0.18 0.11 0.09 – 0.09 0.06 0 0.05 0 0.46 0.47 0.44 0.54 0.65 0.7 0.06 0.06 0.05 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.09 0.05 0 0 0 0 0 0
Endosome 0.05 0.05 0 0 0.06 0 – 0 0 0 0 0 0.05 0.12 0.2 0.22 0.09 0.08 0.07 0.06 0.09 0.14 0.09 0.06
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.61 0.59 0.84 0.89 0.85 0.91 – 0.87 0.94 0.92 0.93 0.96 0.48 0.39 0.23 0.08 0.1 0.06 0.67 0.66 0.68 0.59 0.65 0.7
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0.13 0 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0.08 0 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.11 0 0 0.05 0.06 – 0 0 0.05 0 0 0.11 0.13 0.11 0.15 0.12 0.11 0.1 0.11 0.05 0.06 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 0 1 0 1 0 – 0 0 0 0 0 1 3 0 0 0 0 4 0 1 3 4 5
Bud 5 0 5 8 5 11 – 27 13 15 13 14 3 6 3 2 6 1 2 0 0 1 4 0
Bud Neck 1 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 2 0 4 1 0 1 3 2
Bud Site 0 0 1 0 0 6 – 10 7 7 7 17 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 0 3 2 1 – 0 0 1 2 2 0 1 8 9 2 4 0 0 0 0 0 0
Cytoplasm 127 27 19 27 16 26 – 40 19 14 15 12 63 77 116 97 124 110 30 7 5 5 6 5
Endoplasmic Reticulum 6 5 4 2 6 5 – 1 0 1 0 2 6 2 0 9 18 8 6 1 0 0 1 0
Endosome 22 4 0 0 9 2 – 3 1 1 0 0 7 19 52 39 18 12 35 6 8 19 16 8
Golgi 0 1 0 0 0 0 – 0 0 0 0 0 0 0 2 1 1 0 2 2 1 4 1 1
Mitochondria 254 52 76 133 120 256 – 392 300 298 280 326 65 64 60 14 19 10 347 79 66 86 130 107
Nucleus 5 0 0 0 0 4 – 9 8 5 2 4 0 0 0 2 1 0 3 0 0 0 1 2
Nuclear Periphery 1 1 0 0 1 1 – 5 2 2 6 5 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 1 0 0 1 5 – 10 4 1 6 6 0 1 1 1 0 1 4 0 1 1 1 0
Peroxisomes 1 0 0 1 7 6 – 8 3 4 2 4 0 6 35 8 4 8 2 0 2 3 4 2
SpindlePole 9 2 2 3 4 12 – 37 10 14 16 11 0 2 11 6 5 10 12 1 1 4 4 2
Vac/Vac Membrane 60 10 0 3 7 16 – 14 13 17 8 9 15 22 28 27 23 17 49 13 5 8 14 10
Unique Cell Count 414 88 91 150 141 281 451 320 325 301 341 136 164 265 180 192 157 517 120 98 146 199 155
Labelled Cell Count 499 104 108 180 179 351 557 380 380 357 412 160 203 316 215 223 181 517 120 98 146 199 155


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.6 3.2 3.3 4.0 3.3 – 3.6 3.0 3.6 3.2 3.4 4.5 4.8 4.8 6.1 5.5 6.1 4.5 4.7 5.0
Std Deviation (1e-4) 0.6 0.7 0.6 1.2 1.3 1.3 – 1.5 1.5 1.5 0.8 1.3 0.7 1.0 1.2 2.1 1.7 2.6 1.3 1.1 1.0
Intensity Change (Log2) – – – 0.04 0.33 0.04 – 0.16 -0.08 0.17 -0.02 0.1 0.5 0.59 0.6 0.95 0.77 0.94 0.5 0.55 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.9 3.4 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0581 -0.661 -0.6149 -1.2936 -0.3192 -0.7978 -0.5491 -0.4305 -0.5191 -0.6332 -0.635 -0.6883 0.8678 0.8808 0.5035 0.7484 1.0998 0.8442
Actin 0.0054 0.0035 0.0111 0.0019 0.0025 0.005 0.0268 0.0033 0.0194 0.025 0.0077 0.0233 0.0411 0.0027 0.0153 0.0017 0.0243 0.0155
Bud 0.0008 0.0034 0.0011 0.0013 0.0002 0.0008 0.0031 0.0012 0.012 0.0037 0.0035 0.005 0.0008 0.0023 0.0024 0.0005 0.0027 0.0013
Bud Neck 0.0026 0.0034 0.0036 0.0015 0.0005 0.0266 0.0026 0.001 0.0033 0.0232 0.0032 0.0113 0.0044 0.001 0.0009 0.0007 0.002 0.0098
Bud Periphery 0.0021 0.0066 0.0024 0.002 0.0006 0.0024 0.0049 0.002 0.0093 0.0058 0.0051 0.0077 0.0018 0.0046 0.0101 0.001 0.0059 0.0034
Bud Site 0.0069 0.0295 0.0173 0.0016 0.0006 0.002 0.0165 0.0208 0.0137 0.011 0.0032 0.0032 0.0053 0.0165 0.0143 0.0009 0.0027 0.001
Cell Periphery 0.0016 0.0015 0.0011 0.0005 0.0004 0.0009 0.002 0.0013 0.0021 0.0006 0.0009 0.0012 0.0016 0.0017 0.0028 0.0008 0.0003 0.0006
Cytoplasm 0.113 0.1274 0.1159 0.2113 0.0715 0.2479 0.0891 0.1461 0.1548 0.086 0.1304 0.1734 0.1215 0.1956 0.117 0.1245 0.1304 0.1994
Cytoplasmic Foci 0.1588 0.1309 0.1131 0.1365 0.2855 0.149 0.1048 0.0782 0.1215 0.1249 0.1502 0.1392 0.1183 0.1295 0.1113 0.1491 0.1414 0.0963
Eisosomes 0.002 0.0007 0.0004 0.0003 0.0001 0.0003 0.001 0.0009 0.0011 0.0005 0.0003 0.0005 0.0022 0.0007 0.0017 0.0004 0.0004 0.0004
Endoplasmic Reticulum 0.0199 0.0069 0.004 0.0033 0.0568 0.0047 0.0096 0.0046 0.0076 0.0058 0.002 0.0048 0.0076 0.0082 0.0059 0.0038 0.0048 0.0097
Endosome 0.1619 0.0905 0.1032 0.1221 0.1505 0.1005 0.1114 0.081 0.0808 0.0869 0.1052 0.1126 0.0771 0.0943 0.0941 0.1076 0.123 0.0867
Golgi 0.0216 0.0217 0.0197 0.022 0.0369 0.0275 0.0194 0.013 0.0223 0.0287 0.0255 0.0337 0.0221 0.0184 0.0236 0.0262 0.0374 0.0202
Lipid Particles 0.0634 0.0179 0.0321 0.0099 0.0176 0.0094 0.0237 0.0107 0.0157 0.0147 0.0088 0.0149 0.0221 0.0113 0.0141 0.0087 0.0148 0.0199
Mitochondria 0.101 0.1132 0.1075 0.1564 0.0236 0.0757 0.0893 0.0611 0.118 0.1445 0.1493 0.1549 0.0385 0.0782 0.1098 0.0738 0.237 0.0774
None 0.2905 0.3996 0.3999 0.2939 0.2135 0.3233 0.416 0.5539 0.3647 0.3706 0.3627 0.2679 0.4783 0.3912 0.429 0.4301 0.1693 0.4204
Nuclear Periphery 0.0055 0.0015 0.0019 0.0012 0.0035 0.0007 0.0155 0.0012 0.003 0.0015 0.0012 0.0016 0.0081 0.0042 0.0024 0.0008 0.0173 0.0052
Nucleolus 0.0016 0.0016 0.0011 0.0003 0.0002 0.0004 0.0026 0.0012 0.0017 0.0018 0.0012 0.001 0.0016 0.0012 0.0012 0.0005 0.0019 0.0007
Nucleus 0.0021 0.0017 0.0019 0.0011 0.0007 0.0015 0.0166 0.0016 0.0037 0.0019 0.0023 0.0032 0.0071 0.0022 0.0022 0.0011 0.0058 0.0018
Peroxisomes 0.0136 0.0188 0.0289 0.0116 0.1223 0.0124 0.0252 0.005 0.0246 0.0505 0.0165 0.0253 0.0217 0.0187 0.0282 0.0565 0.0318 0.0172
Punctate Nuclear 0.0028 0.0026 0.0025 0.0008 0.0007 0.0026 0.0057 0.0022 0.0064 0.0024 0.0025 0.005 0.0093 0.0014 0.0018 0.0016 0.0364 0.006
Vacuole 0.0184 0.0148 0.0197 0.0151 0.0085 0.0044 0.0108 0.0078 0.0112 0.0071 0.0135 0.0068 0.0079 0.0124 0.009 0.0067 0.0065 0.0052
Vacuole Periphery 0.0045 0.0023 0.0115 0.0054 0.0031 0.0018 0.0033 0.0017 0.0034 0.0029 0.0048 0.0034 0.0017 0.0036 0.0029 0.003 0.0038 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.8943 4.2153 4.6919 5.4014 9.1706 9.4256 7.9914 2.8766 8.634 6.7687
Translational Efficiency 2.2193 2.3327 1.6872 2.4968 1.2653 1.8681 0.7299 6.9625 1.408 1.5523

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1906 1425 119 1381 1580 1783 184 1519 3486 3208 303 2900

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 664.54 692.77 973.86 832.66 672.89 755.52 904.11 808.72 668.32 727.65 931.50 820.12
Standard Deviation 72.82 102.14 107.77 109.03 79.55 97.47 89.04 116.40 76.06 104.34 102.64 113.58
Intensity Change Log 2 — 0.060020 0.551358 0.325371 — 0.167099 0.426128 0.265270 — 0.114887 0.489711 0.295446

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000391 0.002072 0.005585 0.016389 0.000569 0.001187 0.001702 0.011765 0.000471 0.001580 0.003227 0.013967
Bud Neck 0.044138 0.029554 0.010225 0.039421 0.031395 0.040633 0.014680 0.044504 0.038362 0.035711 0.012931 0.042083
Bud Site 0.013895 0.030480 0.026306 0.116902* 0.011048 0.019842 0.014209 0.089880 0.012605 0.024568 0.018960 0.102748*
Cell Periphery 0.000216 0.000261 0.000327 0.000314 0.000224 0.000339 0.000330 0.000296 0.000219 0.000304 0.000329 0.000305
Cytoplasm 0.122555* 0.043889 0.113030* 0.194451* 0.067189 0.051178 0.176755* 0.204050* 0.097461 0.047940 0.151728* 0.199479*
Cytoplasmic Foci 0.340440* 0.183598* 0.188843* 0.142802 0.229537* 0.208828* 0.172451 0.149813 0.290174* 0.197621* 0.178889 0.146474
Eisosomes 0.000305 0.000215 0.000213 0.000230 0.000256 0.000256 0.000239 0.000234 0.000283 0.000238 0.000229 0.000232
Endoplasmic Reticulum 0.002300 0.000664 0.009016 0.005007 0.001710 0.000990 0.006421 0.003531 0.002033 0.000846 0.007440 0.004234
Endosome 0.072922 0.084152 0.098327 0.023212 0.068111 0.092775 0.048464 0.037482 0.070741 0.088944 0.068047 0.030687
Golgi 0.045401 0.092042 0.037193 0.011969 0.070983 0.099209 0.009187 0.014010 0.056996 0.096026 0.020186 0.013038
Lipid Particles 0.035009 0.011173 0.025343 0.005953 0.022931 0.014213 0.025506 0.006528 0.029535 0.012863 0.025442 0.006254
Mitochondria 0.183008* 0.353350* 0.016950 0.031592 0.370324* 0.326312* 0.007523 0.044200 0.267907* 0.338323* 0.011225 0.038196
Mitotic Spindle 0.003087 0.015944 0.061364 0.071808 0.005444 0.013082 0.011740 0.047062 0.004155 0.014353 0.031229 0.058846
None 0.001530 0.001440 0.001347 0.000781 0.002217 0.000848 0.001557 0.001133 0.001842 0.001111 0.001474 0.000965
Nuclear Periphery 0.000554 0.000242 0.003157 0.000895 0.000421 0.000326 0.003632 0.001404 0.000493 0.000288 0.003445 0.001162
Nuclear Periphery Foci 0.000320 0.000349 0.006624 0.001063 0.000239 0.000253 0.004692 0.001507 0.000283 0.000296 0.005451 0.001296
Nucleolus 0.001890 0.003238 0.018574 0.001148 0.001685 0.001520 0.011287 0.001953 0.001797 0.002283 0.014149 0.001570
Nucleus 0.031126 0.012846 0.168528* 0.064277 0.021989 0.010318 0.304725* 0.074624 0.026985 0.011441 0.251235* 0.069696
Peroxisomes 0.042062 0.089710 0.082285 0.111895* 0.042396 0.071610 0.051046 0.093003 0.042213 0.079650 0.063315 0.101999*
Vacuole 0.047969 0.034228 0.110585 0.156413 0.039526 0.035554 0.129645 0.165978 0.044143 0.034965 0.122159 0.161423
Vacuole Periphery 0.010882 0.010552 0.016176 0.003477 0.011808 0.010725 0.004213 0.007044 0.011301 0.010648 0.008911 0.005345

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.90 -3.17 -9.31 -7.95 -3.81 -2.88 -3.32 -7.53 -7.00 -6.40 -5.77 -4.08 -11.92 -10.62 -7.42
Bud Neck 4.81 11.49 1.91 -2.98 -9.83 -2.69 4.80 -3.17 -0.19 -8.09 1.05 10.83 -0.75 -1.80 -11.90
Bud Site -6.54 -2.69 -19.44 -13.91 -11.65 -5.49 -1.51 -17.49 -14.74 -14.26 -8.34 -2.83 -26.02 -20.38 -18.78
Cell Periphery -1.83 -2.73 -2.11 -0.39 1.37 -2.67 -2.45 -1.30 1.82 1.57 -3.35 -3.77 -2.40 1.37 2.08
Cytoplasm 15.90 0.67 -10.55 -23.46 -5.38 3.45 -6.49 -19.58 -23.05 -2.15 14.34 -4.36 -20.60 -32.73 -4.58
Cytoplasmic Foci 21.58 10.26 32.09 8.88 3.49 2.85 5.24 13.11 10.78 1.44 17.79 12.07 32.23 14.08 3.41
Eisosomes 3.57 2.63 3.76 0.20 0.71 0.00 0.08 1.37 1.33 1.48 2.31 2.02 3.83 1.23 1.66
Endoplasmic Reticulum 3.70 -6.10 -4.70 -10.41 3.74 2.34 -6.46 -5.32 -7.16 4.18 4.12 -8.56 -6.41 -12.32 5.38
Endosome -2.56 -1.97 18.71 17.74 7.01 -5.66 3.77 10.59 15.78 2.00 -5.90 1.11 20.07 23.56 6.67
Golgi -10.66 1.65 16.13 21.38 3.54 -5.98 17.41 18.51 23.28 -1.79 -12.21 11.10 24.15 31.64 2.30
Lipid Particles 10.22 1.91 14.30 4.16 4.46 4.62 -0.01 10.36 6.52 4.08 10.90 1.68 17.58 7.65 5.95
Mitochondria -16.04 24.41 26.28 35.83* -2.18 3.96 42.81* 38.08* 35.57* -9.66 -8.93 47.32* 43.76* 50.51* -8.47
Mitotic Spindle -8.55 -4.46 -14.38 -10.90 -0.43 -4.23 -1.53 -11.33 -8.47 -6.80 -8.60 -4.63 -18.34 -13.74 -3.92
None 0.12 0.30 3.46 1.99 2.31 1.96 0.85 1.55 -1.17 1.55 1.91 0.80 2.53 0.79 2.70
Nuclear Periphery 3.68 -2.41 -4.08 -6.41 1.80 1.01 -2.99 -5.18 -5.37 1.80 3.05 -3.80 -6.47 -7.82 2.56
Nuclear Periphery Foci -0.22 -5.35 -8.69 -5.61 4.59 -0.25 -4.40 -3.55 -3.49 2.67 -0.20 -6.77 -5.43 -5.20 5.01
Nucleolus -1.86 -3.22 2.48 2.64 3.32 0.02 -3.14 -1.17 -1.30 2.96 -1.53 -4.47 -0.01 1.51 4.47
Nucleus 7.63 -6.28 -12.03 -17.05 4.27 5.61 -12.95 -15.15 -18.92 9.83 9.90 -13.83 -19.11 -25.53 10.35
Peroxisomes -10.35 -2.68 -10.05 -0.40 -0.85 -7.70 -0.44 -8.48 -2.01 -3.72 -12.84 -2.22 -13.14 -1.79 -3.33
Vacuole 4.34 -5.58 -24.85 -26.70 -6.88 1.27 -8.91 -25.82 -26.64 -2.79 4.11 -10.32 -35.71 -37.69 -6.08
Vacuole Periphery 0.41 -0.97 12.43 9.32 2.98 1.14 7.53 5.97 5.28 -2.20 1.04 1.94 11.60 10.01 2.06
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene
Localization
Cell Percentages mitochondrion (70%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Rso55

Rso55


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rso55-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available